Restriction map for
pFN21AB3279
(Linear) (Six-base) MAPSORT of: KIBB3279.seq Check: 8165 from: 1 to: 2850
KIBB3279.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1140 1644 2850
Size: 1140 504 1206
Fragments arranged by size:
1206 1140 504
AccI GT'mk_AC
Cuts at: 0 329 739 2850
Size: 329 410 2111
Fragments arranged by size:
2111 410 329
AclI AA'CG_TT
Cuts at: 0 761 2850
Size: 761 2089
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2298 2850
Size: 2298 552
AfeI AGC'GCT
Cuts at: 0 1504 1973 2850
Size: 1504 469 877
Fragments arranged by size:
1504 877 469
AflII C'TTAA_G
Cuts at: 0 891 2850
Size: 891 1959
AflIII A'CryG_T
Cuts at: 0 1520 1811 1900 2090 2850
Size: 1520 291 89 190 760
Fragments arranged by size:
1520 760 291 190 89
AleI CACnn'nnGTG
Cuts at: 0 130 2850
Size: 130 2720
AlwNI CAG_nnn'CTG
Cuts at: 0 153 2850
Size: 153 2697
ApaI G_GGCC'C
Cuts at: 0 2065 2850
Size: 2065 785
ApaLI G'TGCA_C
Cuts at: 0 1528 2458 2850
Size: 1528 930 392
Fragments arranged by size:
1528 930 392
ApoI r'AATT_y
Cuts at: 0 258 2659 2850
Size: 258 2401 191
Fragments arranged by size:
2401 258 191
AvaI C'yCGr_G
Cuts at: 0 658 1800 2065 2319 2850
Size: 658 1142 265 254 531
Fragments arranged by size:
1142 658 531 265 254
BanI G'GyrC_C
Cuts at: 0 124 177 670 1756 2022 2053 2850
Size: 124 53 493 1086 266 31 797
Fragments arranged by size:
1086 797 493 266 124 53 31
BanII G_rGCy'C
Cuts at: 0 520 1236 1294 2065 2850
Size: 520 716 58 771 785
Fragments arranged by size:
785 771 716 520 58
BbeI G_GCGC'C
Cuts at: 0 1760 2026 2850
Size: 1760 266 824
Fragments arranged by size:
1760 824 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 2302 2850
Size: 2302 548
BciVI GTATCCnnnnn_n'
Cuts at: 0 2535 2850
Size: 2535 315
BclI T'GATC_A
Cuts at: 0 2099 2505 2850
Size: 2099 406 345
Fragments arranged by size:
2099 406 345
Bme1580I G_kGCm'C
Cuts at: 0 1532 1717 1908 2065 2462 2850
Size: 1532 185 191 157 397 388
Fragments arranged by size:
1532 397 388 191 185 157
BmgBI CAC'GTC
Cuts at: 0 360 543 1176 1341 2850
Size: 360 183 633 165 1509
Fragments arranged by size:
1509 633 360 183 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 712 949 1102 2396 2850
Size: 712 237 153 1294 454
Fragments arranged by size:
1294 712 454 237 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 244 337 374 828 842 1603 2552
Size: 181 63 93 37 454 14 761 949
Cuts at: 2552 2850
Size: 298
Fragments arranged by size:
949 761 454 298 181 93 63 37
14
BsaI GGTCTCn'nnnn_
Cuts at: 0 2755 2850
Size: 2755 95
BsaAI yAC'GTr
Cuts at: 0 1521 2850
Size: 1521 1329
BsaHI Gr'CG_yC
Cuts at: 0 1168 1757 1793 2023 2044 2850
Size: 1168 589 36 230 21 806
Fragments arranged by size:
1168 806 589 230 36 21
BsaWI w'CCGG_w
Cuts at: 0 615 2635 2850
Size: 615 2020 215
Fragments arranged by size:
2020 615 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 826 856 1477 1507 2413 2443 2850
Size: 826 30 621 30 906 30 407
Fragments arranged by size:
906 826 621 407 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 32 292 1591 2274 2482 2773 2850
Size: 32 260 1299 683 208 291 77
Fragments arranged by size:
1299 683 291 260 208 77 32
BseYI C'CCAG_C
Cuts at: 0 399 2850
Size: 399 2451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 2850
Size: 324 2526
BsiEI CG_ry'CG
Cuts at: 0 273 1750 2850
Size: 273 1477 1100
Fragments arranged by size:
1477 1100 273
BsiHKAI G_wGCw'C
Cuts at: 0 945 1236 1532 2170 2278 2462 2850
Size: 945 291 296 638 108 184 388
Fragments arranged by size:
945 638 388 296 291 184 108
BsmI GAATG_Cn'
Cuts at: 0 2477 2850
Size: 2477 373
Bsp1286I G_dGCh'C
Cuts at: 0 520 945 1236 1294 1532 1717 1908 2065
Size: 520 425 291 58 238 185 191 157
Cuts at: 2065 2170 2278 2462 2850
Size: 105 108 184 388
Fragments arranged by size:
520 425 388 291 238 191 185 184
157 108 105 58
BspEI T'CCGG_A
Cuts at: 0 615 2850
Size: 615 2235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 552 1140 1165 1644 2850
Size: 333 219 588 25 479 1206
Fragments arranged by size:
1206 588 479 333 219 25
BsrBI CCG'CTC
Cuts at: 0 83 1488 1541 2015 2850
Size: 83 1405 53 474 835
Fragments arranged by size:
1405 835 474 83 53
BsrDI GCAATG_nn'
Cuts at: 0 71 2850
Size: 71 2779
BsrFI r'CCGG_y
Cuts at: 0 1686 1759 2850
Size: 1686 73 1091
Fragments arranged by size:
1686 1091 73
BsrGI T'GTAC_A
Cuts at: 0 1000 1877 2150 2850
Size: 1000 877 273 700
Fragments arranged by size:
1000 877 700 273
BssHII G'CGCG_C
Cuts at: 0 1607 1609 1782 2195 2850
Size: 1607 2 173 413 655
Fragments arranged by size:
1607 655 413 173 2
BssSI C'ACGA_G
Cuts at: 0 1573 2850
Size: 1573 1277
BstEII G'GTnAC_C
Cuts at: 0 1152 1272 2850
Size: 1152 120 1578
Fragments arranged by size:
1578 1152 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1995 2850
Size: 1995 855
BstYI r'GATC_y
Cuts at: 0 21 950 2341 2674 2850
Size: 21 929 1391 333 176
Fragments arranged by size:
1391 929 333 176 21
BstZ17I GTA'TAC
Cuts at: 0 740 2850
Size: 740 2110
BtgI C'CryG_G
Cuts at: 0 210 1310 1959 1982 2057 2850
Size: 210 1100 649 23 75 793
Fragments arranged by size:
1100 793 649 210 75 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 408 2088 2188 2850
Size: 126 282 1680 100 662
Fragments arranged by size:
1680 662 282 126 100
BtsI GCAGTG_nn'
Cuts at: 0 1733 1793 2518 2850
Size: 1733 60 725 332
Fragments arranged by size:
1733 725 332 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 53 88 2850
Size: 53 35 2762
Fragments arranged by size:
2762 53 35
DraI TTT'AAA
Cuts at: 0 2607 2850
Size: 2607 243
EaeI y'GGCC_r
Cuts at: 0 84 638 1313 1334 1844 1979 1985 2016
Size: 84 554 675 21 510 135 6 31
Cuts at: 2016 2515 2850
Size: 499 335
Fragments arranged by size:
675 554 510 499 335 135 84 31
21 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 184 306 1245 2850
Size: 184 122 939 1605
Fragments arranged by size:
1605 939 184 122
EcoICRI GAG'CTC
Cuts at: 0 1234 2850
Size: 1234 1616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 244 337 374 828 842 1603 2298
Size: 181 63 93 37 454 14 761 695
Cuts at: 2298 2552 2850
Size: 254 298
Fragments arranged by size:
761 695 454 298 254 181 93 63
37 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 2467 2850
Size: 223 2244 383
Fragments arranged by size:
2244 383 223
EcoO109I rG'GnC_Cy
Cuts at: 0 150 216 2062 2500 2850
Size: 150 66 1846 438 350
Fragments arranged by size:
1846 438 350 150 66
EcoRI G'AATT_C
Cuts at: 0 258 2850
Size: 258 2592
EcoRV GAT'ATC
Cuts at: 0 483 2850
Size: 483 2367
HaeII r_GCGC'y
Cuts at: 0 1506 1693 1760 1938 1975 2026 2850
Size: 1506 187 67 178 37 51 824
Fragments arranged by size:
1506 824 187 178 67 51 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 853 885 1237 1269 1276 1308
Size: 454 32 367 32 352 32 7 32
Cuts at: 1308 1783 1815 2850
Size: 475 32 1035
Fragments arranged by size:
1035 475 454 367 352 32 32 32
32 32 7
HincII GTy'rAC
Cuts at: 0 1049 2088 2586 2850
Size: 1049 1039 498 264
Fragments arranged by size:
1049 1039 498 264
KasI G'GCGC_C
Cuts at: 0 1756 2022 2850
Size: 1756 266 828
Fragments arranged by size:
1756 828 266
MluI A'CGCG_T
Cuts at: 0 1811 1900 2850
Size: 1811 89 950
Fragments arranged by size:
1811 950 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1547 2850
Size: 176 1371 1303
Fragments arranged by size:
1371 1303 176
MscI TGG'CCA
Cuts at: 0 2517 2850
Size: 2517 333
MslI CAynn'nnrTG
Cuts at: 0 130 791 2850
Size: 130 661 2059
Fragments arranged by size:
2059 661 130
MspA1I CmG'CkG
Cuts at: 0 212 339 2597 2850
Size: 212 127 2258 253
Fragments arranged by size:
2258 253 212 127
NaeI GCC'GGC
Cuts at: 0 1688 1761 2850
Size: 1688 73 1089
Fragments arranged by size:
1688 1089 73
NarI GG'CG_CC
Cuts at: 0 1757 2023 2850
Size: 1757 266 827
Fragments arranged by size:
1757 827 266
NdeI CA'TA_TG
Cuts at: 0 920 2850
Size: 920 1930
NgoMIV G'CCGG_C
Cuts at: 0 1686 1759 2850
Size: 1686 73 1091
Fragments arranged by size:
1686 1091 73
NspI r_CATG'y
Cuts at: 0 2448 2850
Size: 2448 402
PfoI T'CCnGG_A
Cuts at: 0 104 536 2493 2850
Size: 104 432 1957 357
Fragments arranged by size:
1957 432 357 104
PpuMI rG'GwC_Cy
Cuts at: 0 150 216 2850
Size: 150 66 2634
Fragments arranged by size:
2634 150 66
PspOMI G'GGCC_C
Cuts at: 0 2061 2850
Size: 2061 789
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 723 755 1369 1401 2850
Size: 723 32 614 32 1449
Fragments arranged by size:
1449 723 614 32 32
PstI C_TGCA'G
Cuts at: 0 239 344 1562 1655 1835 2850
Size: 239 105 1218 93 180 1015
Fragments arranged by size:
1218 1015 239 180 105 93
PvuII CAG'CTG
Cuts at: 0 2597 2850
Size: 2597 253
SacI G_AGCT'C
Cuts at: 0 1236 2850
Size: 1236 1614
SacII CC_GC'GG
Cuts at: 0 213 2850
Size: 213 2637
SfcI C'TryA_G
Cuts at: 0 235 340 1558 1591 1651 1831 2345 2850
Size: 235 105 1218 33 60 180 514 505
Fragments arranged by size:
1218 514 505 235 180 105 60 33
SfoI GGC'GCC
Cuts at: 0 1758 2024 2850
Size: 1758 266 826
Fragments arranged by size:
1758 826 266
SgrAI Cr'CCGG_yG
Cuts at: 0 1686 1759 2850
Size: 1686 73 1091
Fragments arranged by size:
1686 1091 73
SmaI CCC'GGG
Cuts at: 0 2321 2850
Size: 2321 529
SmlI C'TyrA_G
Cuts at: 0 9 891 2850
Size: 9 882 1959
Fragments arranged by size:
1959 882 9
SphI G_CATG'C
Cuts at: 0 2448 2850
Size: 2448 402
SrfI GCCC'GGGC
Cuts at: 0 2321 2850
Size: 2321 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 60 2041 2314 2850
Size: 60 1981 273 536
Fragments arranged by size:
1981 536 273 60
TatI w'GTAC_w
Cuts at: 0 1000 1376 1877 2150 2850
Size: 1000 376 501 273 700
Fragments arranged by size:
1000 700 501 376 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 764 796 935 967 2850
Size: 764 32 139 32 1883
Fragments arranged by size:
1883 764 139 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 879 1323 2850
Size: 879 444 1527
Fragments arranged by size:
1527 879 444
XmaI C'CCGG_G
Cuts at: 0 2319 2850
Size: 2319 531
XmnI GAAnn'nnTTC
Cuts at: 0 418 2850
Size: 418 2432
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AleI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BciVI BclI Bme1580I BmgBI BmrI BpmI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI EaeI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PfoI PpuMI PspOMI PsrI PstI PvuII
SacI SacII SfcI SfoI SgrAI SmaI SmlI SphI
SrfI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI AhdI AlfI AloI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BfrBI BglI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI BsiWI
BsmBI BspHI BstAPI BstBI Bsu36I ClaI DraIII DrdI
EagI EarI FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SnaBI SpeI SspI StuI StyI
SwaI Tth111I XbaI XhoI ZraI