Restriction map for
pFN21AB3253
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BcgI BclI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRV FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 1490 ACATGT A^C[GA][CT]GT
AvaI 912 CTCGGG C^[CT]CG[GA]G
AvaII 833 GGTCC G^G[AT]CC
AvrII 1522 CCTAGG C^CTAGG
BanI 638 GGTGCC G^G[CT][GA]CC
BbvCI 391 GCTGAGG CCTCAGC(-5/-2)
BciVI 711 GGATAC GTATCC(6/5)
BglII 315 AGATCT A^GATCT
BlnI 1522 CCTAGG C^CTAGG
BpmI 643 CTGGAG CTGGAG(16/14)
BsmAI 1177 GTCTC GTCTC(1/5)
BsmFI 452 GGGAC GGGAC(10/14)
BsmI 729 GAATGC GAATGC(1/-1)
BsoBI 912 CTCGGG C^[CT]CG[GA]G
BspHI 827 TCATGA T^CATGA
BspLU11I 1490 ACATGT A^CATGT
BspMI 1818 ACCTGC ACCTGC(4/8)
BsrDI 733 GCAATG GCAATG(2/0)
BstAPI 1716 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1745 TTCGAA TT^CGAA
BstEII 1162 GGTGACC G^GT[ACGT]ACC
BstPI 1162 GGTGACC G^GT[ACGT]ACC
Cfr13I 833 GGTCC G^G[ACGT]CC
DraI 1708 TTTAAA TTT^AAA
EcoO65I 1162 GGTGACC G^GT[ACGT]ACC
EcoRI 1246 GAATTC G^AATTC
EcoT22I 323 ATGCAT ATGCA^T
MaeII 704 ACGT A^CGT
MspA1I 749 CAGCTG C[AC]G^C[GT]G
NsiI 323 ATGCAT ATGCA^T
NspI 1490 ACATGT [GA]CATG^[CT]
NspV 1745 TTCGAA TT^CGAA
PflMI 616 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 18 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 950 CTGCAG CTGCA^G
PvuII 749 CAGCTG CAG^CTG
RcaI 827 TCATGA T^CATGA
Sau96I 833 GGTCC G^G[ACGT]CC
ScaI 1400 AGTACT AGT^ACT
SfuI 1745 TTCGAA TT^CGAA
SmlI 1342 CTCAAG C^T[CT][GA]AG
Tsp45I 1163 GTGAC ^GT[GC]AC
Van91I 616 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 418 CCAAACTCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 11 TCCGGA T^CCGGA
539 TCCGGA
AcsI 1132 AAATTT [GA]^AATT[CT]
1246 GAATTC
AlwI 1272 GATCC GGATC(4/5)
1115 GGATC
AlwNI 1717 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1814 CAGAACCTG
ApoI 1132 AAATTT [GA]^AATT[CT]
1246 GAATTC
AspHI 909 GAGCTC G[AT]GC[AT]^C
1339 GAGCTC
BanII 909 GAGCTC G[GA]GC[CT]^C
1339 GAGCTC
BfaI 1523 CTAG C^TAG
1597 CTAG
BmyI 909 GAGCTC G[AGT]GC[ACT]^C
1339 GAGCTC
BseAI 11 TCCGGA T^CCGGA
539 TCCGGA
BsiHKAI 909 GAGCTC G[AT]GC[AT]^C
1339 GAGCTC
Bsp1286I 909 GAGCTC G[AGT]GC[ACT]^C
1339 GAGCTC
BspEI 11 TCCGGA T^CCGGA
539 TCCGGA
BstXI 635 CCAGGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1152 CCAGTTGGATGG
Cac8I 371 GCTGGC GC[ACGT]^[ACGT]GC
505 GCAAGC
EcoNI 1067 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1644 CCTCACCAAGG
MaeI 1523 CTAG C^TAG
1597 CTAG
MaeIII 1163 GTGAC ^GT[ACGT]AC
1659 GTTAC
MroI 11 TCCGGA T^CCGGA
539 TCCGGA
NlaIV 638 GGTGCC GG[ACGT]^[ACGT]CC
1449 GGAGCC
PleI 118 GACTC GAGTC(4/5)
1719 GACTC
SacI 909 GAGCTC GAGCT^C
1339 GAGCTC
SapI 1336 GAAGAGC GCTCTTC(1/4)
1741 GCTCTTC
SspI 787 AATATT AAT^ATT
817 AATATT
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AfaI AluI Asp700I BalI BbsI
BbvI BcnI BpuAI BsaJI BsaWI
BsiYI BslI BsrI BssKI BstF5I
BstNI BstYI DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MboI MboII MflI
MluNI MnlI MscI MseI MspI
MvaI MwoI NciI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI