(Circular) (Six-base) MAPSORT of: pFN21AB3253.seq Check: 6308 from: 1 to: 6568 pFN21AB3253 6568 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3911 1937 Size: 1974 4594 Acc65I G'GTAC_C Cuts at: 3889 3889 Size: 6568 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2212 2831 3257 4631 6015 6283 1632 Size: 580 619 426 1374 1384 268 1917 Fragments arranged by size: 1917 1384 1374 619 580 426 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6371 AflIII A'CryG_T Cuts at: 1203 3495 5467 6444 1203 Size: 2292 1972 977 1327 Fragments arranged by size: 2292 1972 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6568 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6568 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3508 3540 3508 Size: 32 6536 AlwNI CAG_nnn'CTG Cuts at: 3727 3824 4311 5883 3727 Size: 97 487 1572 4412 Fragments arranged by size: 4412 1572 487 97 ApaLI G'TGCA_C Cuts at: 4625 5781 4625 Size: 1156 5412 ApoI r'AATT_y Cuts at: 1493 3137 3251 3877 4103 4255 6364 1493 Size: 1644 114 626 226 152 2109 1697 Fragments arranged by size: 2109 1697 1644 626 226 152 114 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 2917 3893 1870 Size: 73 974 976 4545 Fragments arranged by size: 4545 976 974 73 AvrII C'CTAG_G Cuts at: 3527 3527 Size: 6568 BamHI G'GATC_C Cuts at: 3898 4265 3898 Size: 367 6201 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2643 3889 5344 618 Size: 325 217 627 856 1246 1455 1842 Fragments arranged by size: 1842 1455 1246 856 627 325 217 BanII G_rGCy'C Cuts at: 1391 2918 3348 3887 1391 Size: 1527 430 539 4072 Fragments arranged by size: 4072 1527 539 430 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2784 2953 3368 3399 928 Size: 971 885 169 415 31 4097 Fragments arranged by size: 4097 971 885 415 169 31 BbvCI CC'TCA_GC Cuts at: 2397 2397 Size: 6568 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4759 4793 1903 Size: 34 2822 34 3678 Fragments arranged by size: 3678 2822 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2710 4460 5670 2710 Size: 1750 1210 3608 Fragments arranged by size: 3608 1750 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6568 BfrBI ATG'CAT Cuts at: 2330 2330 Size: 6568 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5179 136 Size: 107 122 71 631 605 3507 1525 Fragments arranged by size: 3507 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2320 6563 2320 Size: 4243 2325 BlpI GC'TnA_GC Cuts at: 3985 3985 Size: 6568 Bme1580I G_kGCm'C Cuts at: 1790 4629 5785 1790 Size: 2839 1156 2573 Fragments arranged by size: 2839 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6568 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2558 4299 5257 Size: 416 53 83 399 24 1141 1741 958 Cuts at: 5257 442 Size: 1753 Fragments arranged by size: 1753 1741 1141 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6568 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3362 3362 Size: 6568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 5228 1239 Size: 1430 2559 2579 Fragments arranged by size: 2579 2559 1430 Bpu10I CC'TnA_GC Cuts at: 2397 2397 Size: 6568 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3332 4693 5558 5856 3332 Size: 1361 865 298 4044 Fragments arranged by size: 4044 1361 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5231 882 Size: 617 3732 2219 Fragments arranged by size: 3732 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4359 493 Size: 711 3155 2702 Fragments arranged by size: 3155 2702 711 BsaBI GATnn'nnATC Cuts at: 6562 6562 Size: 6568 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4375 4757 5392 275 Size: 53 83 186 936 2842 382 635 1451 Fragments arranged by size: 2842 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2016 2544 2903 5001 5399 5673 5820 6231 2016 Size: 528 359 2098 398 274 147 411 2353 Fragments arranged by size: 2353 2098 528 411 398 359 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6343 Fragments arranged by size: 6343 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6568 BseYI C'CCAG_C Cuts at: 5771 6267 5771 Size: 496 6072 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5995 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4018 4779 5807 1540 Size: 275 186 2017 761 1028 2301 Fragments arranged by size: 2301 2017 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2918 3348 4629 4714 5785 2918 Size: 430 1281 85 1071 3701 Fragments arranged by size: 3701 1281 1071 430 85 BsmI GAATG_Cn' Cuts at: 2740 4079 4172 2740 Size: 1339 93 5136 Fragments arranged by size: 5136 1339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2918 3348 3887 4629 4714 5785 1391 Size: 399 1128 430 539 742 85 1071 2174 Fragments arranged by size: 2174 1128 1071 742 539 430 399 85 BspEI T'CCGG_A Cuts at: 2016 2544 6231 2016 Size: 528 3687 2353 Fragments arranged by size: 3687 2353 528 BspHI T'CATG_A Cuts at: 2832 4457 6187 2832 Size: 1625 1730 3213 Fragments arranged by size: 3213 1730 1625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3832 3923 844 Size: 2988 91 3489 Fragments arranged by size: 3489 2988 91 BsrBI CCG'CTC Cuts at: 1394 1823 4455 1394 Size: 429 2632 3507 Fragments arranged by size: 3507 2632 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2745 5063 5237 65 Size: 1183 1497 2318 174 1396 Fragments arranged by size: 2318 1497 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5212 5399 1700 Size: 110 3402 187 2869 Fragments arranged by size: 3402 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6568 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6568 BssSI C'ACGA_G Cuts at: 4628 5640 4628 Size: 1012 5556 BstAPI GCAn_nnn'nTGC Cuts at: 794 3727 794 Size: 2933 3635 BstBI TT'CG_AA Cuts at: 3751 5372 3751 Size: 1621 4947 BstEII G'GTnAC_C Cuts at: 1184 3167 1184 Size: 1983 4585 BstXI CCAn_nnnn'nTGG Cuts at: 2647 3164 2647 Size: 517 6051 BstYI r'GATC_y Cuts at: 1972 2320 3120 3276 3898 4265 4650 4667 6119 Size: 348 800 156 622 367 385 17 1452 Cuts at: 6119 6205 6217 6563 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 800 622 385 367 348 346 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5077 Fragments arranged by size: 5077 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5829 Fragments arranged by size: 5829 683 56 BtsI GCAGTG_nn' Cuts at: 3518 4084 4878 4898 3518 Size: 566 794 20 5188 Fragments arranged by size: 5188 794 566 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 3715 3872 3715 Size: 157 6411 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5575 809 Size: 507 4259 1802 Fragments arranged by size: 4259 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2742 2967 3154 Size: 54 1425 50 275 18 912 225 187 Cuts at: 3154 3377 4015 4904 6533 8 Size: 223 638 889 1629 43 Fragments arranged by size: 1629 1425 912 889 638 275 225 223 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4015 1537 Size: 275 2203 4090 Fragments arranged by size: 4090 2203 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3336 3753 3798 4498 1353 Size: 1983 417 45 700 3423 Fragments arranged by size: 3423 1983 700 417 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5155 5529 5675 387 Size: 1064 138 3566 374 146 1280 Fragments arranged by size: 3566 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2916 3346 2916 Size: 430 6138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2669 2831 3257 4631 5228 6015 Size: 393 580 457 162 426 1374 597 787 Cuts at: 6015 6283 1239 Size: 268 1524 Fragments arranged by size: 1524 1374 787 597 580 457 426 393 268 162 EcoNI CCTnn'n_nnAGG Cuts at: 3076 3653 3076 Size: 577 5991 EcoRI G'AATT_C Cuts at: 3251 3877 4255 3251 Size: 626 378 5564 Fragments arranged by size: 5564 626 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3284 3424 3879 3284 Size: 140 455 5973 Fragments arranged by size: 5973 455 140 FspI TGC'GCA Cuts at: 5074 5074 Size: 6568 HaeII r_GCGC'y Cuts at: 1678 5715 1678 Size: 4037 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2885 2917 3107 3139 3590 Size: 32 135 32 1313 32 190 32 451 Cuts at: 3590 3622 5209 5241 5283 5315 1373 Size: 32 1587 32 42 32 2626 Fragments arranged by size: 2626 1587 1313 451 190 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3912 4158 669 Size: 988 281 1974 246 3079 Fragments arranged by size: 3079 1974 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6568 HpaI GTT'AAC Cuts at: 4158 4158 Size: 6568 KpnI G_GTAC'C Cuts at: 3893 3893 Size: 6568 MfeI C'AATT_G Cuts at: 4167 4167 Size: 6568 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2791 3141 5682 5866 1305 Size: 1486 350 2541 184 2007 Fragments arranged by size: 2541 2007 1486 350 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2744 2969 3156 3379 10 Size: 54 1425 343 912 225 187 223 3199 Fragments arranged by size: 3199 1425 912 343 225 223 187 54 MslI CAynn'nnrTG Cuts at: 518 1166 4526 4885 5044 518 Size: 648 3360 359 159 2042 Fragments arranged by size: 3360 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2756 3984 4661 5809 6054 1606 Size: 1150 1228 677 1148 245 2120 Fragments arranged by size: 2120 1228 1150 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5077 Fragments arranged by size: 5077 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6568 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NsiI A_TGCA'T Cuts at: 2332 2332 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 3499 3926 5471 1132 Size: 97 2270 427 1545 2229 Fragments arranged by size: 2270 2229 1545 427 97 PasI CC'CwG_GG Cuts at: 1348 3049 1348 Size: 1701 4867 PciI A'CATG_T Cuts at: 3495 5467 3495 Size: 1972 4596 PflMI CCAn_nnn'nTGG Cuts at: 1108 2627 1108 Size: 1519 5049 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4637 4669 6150 6182 4637 Size: 32 1481 32 5023 Fragments arranged by size: 5023 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2027 1613 Size: 414 6154 PsiI TTA'TAA Cuts at: 2340 4138 2340 Size: 1798 4770 PstI C_TGCA'G Cuts at: 830 2959 3920 830 Size: 2129 961 3478 Fragments arranged by size: 3478 2129 961 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 2756 2756 Size: 6568 SacI G_AGCT'C Cuts at: 2918 3348 2918 Size: 430 6138 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SapI GCTCTTCn'nnn_ Cuts at: 3336 3753 3336 Size: 417 6151 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 3407 4816 6528 3407 Size: 1409 1712 3447 Fragments arranged by size: 3447 1712 1409 SfcI C'TryA_G Cuts at: 826 1045 2955 3076 3421 3756 3916 5051 5732 Size: 219 1910 121 345 335 160 1135 681 Cuts at: 5732 5923 826 Size: 191 1471 Fragments arranged by size: 1910 1471 1135 681 345 335 219 191 160 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3895 1872 Size: 2023 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3347 4672 5573 5835 820 Size: 197 926 1404 1325 901 262 1553 Fragments arranged by size: 1553 1404 1325 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4359 493 Size: 3866 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 3926 1132 Size: 2794 3774 SspI AAT'ATT Cuts at: 5 52 2794 2824 4492 5 Size: 47 2742 30 1668 2081 Fragments arranged by size: 2742 2081 1668 47 30 StuI AGG'CCT Cuts at: 1517 1517 Size: 6568 StyI C'CwwG_G Cuts at: 513 1065 2004 2025 3316 3527 3654 4007 513 Size: 552 939 21 1291 211 127 353 3074 Fragments arranged by size: 3074 1291 939 552 353 211 127 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2067 3242 4595 4612 4765 4950 5395 Size: 1255 487 1175 1353 17 153 185 445 Cuts at: 5395 325 Size: 1498 Fragments arranged by size: 1498 1353 1255 1175 487 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3405 4814 6526 Size: 274 80 33 51 1443 1428 1409 1712 Cuts at: 6526 96 Size: 138 Fragments arranged by size: 1712 1443 1428 1409 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3200 3232 6528 6560 3200 Size: 32 3296 32 3208 Fragments arranged by size: 3296 3208 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6568 XbaI T'CTAG_A Cuts at: 3904 3904 Size: 6568 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2430 2430 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6568 XmaI C'CCGG_G Cuts at: 1870 3893 1870 Size: 2023 4545 XmnI GAAnn'nnTTC Cuts at: 2823 3234 3264 4697 2823 Size: 411 30 1433 4694 Fragments arranged by size: 4694 1433 411 30 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI