Restriction map for
pFN21AB3253
(Linear) (Six-base) MAPSORT of: KIBB3253.seq Check: 1825 from: 1 to: 1863
KIBB3253.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 826 1252 1863
Size: 207 619 426 611
Fragments arranged by size:
619 611 426 207
AflIII A'CryG_T
Cuts at: 0 1490 1863
Size: 1490 373
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1503 1535 1863
Size: 1503 32 328
Fragments arranged by size:
1503 328 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1722 1819 1863
Size: 1722 97 44
Fragments arranged by size:
1722 97 44
ApoI r'AATT_y
Cuts at: 0 1132 1246 1863
Size: 1132 114 617
Fragments arranged by size:
1132 617 114
AvaI C'yCGr_G
Cuts at: 0 912 1863
Size: 912 951
AvrII C'CTAG_G
Cuts at: 0 1522 1863
Size: 1522 341
BanI G'GyrC_C
Cuts at: 0 638 1863
Size: 638 1225
BanII G_rGCy'C
Cuts at: 0 913 1343 1863
Size: 913 430 520
Fragments arranged by size:
913 520 430
BbsI GAAGACnn'nnnn_
Cuts at: 0 779 948 1363 1394 1863
Size: 779 169 415 31 469
Fragments arranged by size:
779 469 415 169 31
BbvCI CC'TCA_GC
Cuts at: 0 392 1863
Size: 392 1471
BciVI GTATCCnnnnn_n'
Cuts at: 0 705 1863
Size: 705 1158
BfrBI ATG'CAT
Cuts at: 0 325 1863
Size: 325 1538
BglII A'GATC_T
Cuts at: 0 315 1863
Size: 315 1548
BmrI ACTGGGnnnn_n'
Cuts at: 0 553 1863
Size: 553 1310
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1357 1863
Size: 1357 506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1863
Size: 664 1199
Bpu10I CC'TnA_GC
Cuts at: 0 392 1863
Size: 392 1471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 1863
Size: 1327 536
BsaWI w'CCGG_w
Cuts at: 0 11 539 898 1863
Size: 11 528 359 965
Fragments arranged by size:
965 528 359 11
BsiHKAI G_wGCw'C
Cuts at: 0 913 1343 1863
Size: 913 430 520
Fragments arranged by size:
913 520 430
BsmI GAATG_Cn'
Cuts at: 0 735 1863
Size: 735 1128
Bsp1286I G_dGCh'C
Cuts at: 0 913 1343 1863
Size: 913 430 520
Fragments arranged by size:
913 520 430
BspEI T'CCGG_A
Cuts at: 0 11 539 1863
Size: 11 528 1324
Fragments arranged by size:
1324 528 11
BspHI T'CATG_A
Cuts at: 0 827 1863
Size: 827 1036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1827 1863
Size: 1827 36
BsrDI GCAATG_nn'
Cuts at: 0 740 1863
Size: 740 1123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1722 1863
Size: 1722 141
BstBI TT'CG_AA
Cuts at: 0 1746 1863
Size: 1746 117
BstEII G'GTnAC_C
Cuts at: 0 1162 1863
Size: 1162 701
BstXI CCAn_nnnn'nTGG
Cuts at: 0 642 1159 1863
Size: 642 517 704
Fragments arranged by size:
704 642 517
BstYI r'GATC_y
Cuts at: 0 315 1115 1271 1863
Size: 315 800 156 592
Fragments arranged by size:
800 592 315 156
BtsI GCAGTG_nn'
Cuts at: 0 1513 1863
Size: 1513 350
DraI TTT'AAA
Cuts at: 0 1710 1863
Size: 1710 153
EaeI y'GGCC_r
Cuts at: 0 737 962 1149 1372 1863
Size: 737 225 187 223 491
Fragments arranged by size:
737 491 225 223 187
EarI CTCTTCn'nnn_
Cuts at: 0 1331 1748 1793 1863
Size: 1331 417 45 70
Fragments arranged by size:
1331 417 70 45
EcoICRI GAG'CTC
Cuts at: 0 911 1341 1863
Size: 911 430 522
Fragments arranged by size:
911 522 430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 664 826 1252 1863
Size: 207 457 162 426 611
Fragments arranged by size:
611 457 426 207 162
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1071 1648 1863
Size: 1071 577 215
Fragments arranged by size:
1071 577 215
EcoRI G'AATT_C
Cuts at: 0 1246 1863
Size: 1246 617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1279 1419 1863
Size: 1279 140 444
Fragments arranged by size:
1279 444 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 880 912 1102 1134 1585 1617 1863
Size: 880 32 190 32 451 32 246
Fragments arranged by size:
880 451 246 190 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1136 1863
Size: 786 350 727
Fragments arranged by size:
786 727 350
MscI TGG'CCA
Cuts at: 0 739 964 1151 1374 1863
Size: 739 225 187 223 489
Fragments arranged by size:
739 489 225 223 187
MspA1I CmG'CkG
Cuts at: 0 751 1863
Size: 751 1112
NsiI A_TGCA'T
Cuts at: 0 327 1863
Size: 327 1536
NspI r_CATG'y
Cuts at: 0 1494 1863
Size: 1494 369
PasI CC'CwG_GG
Cuts at: 0 1044 1863
Size: 1044 819
PciI A'CATG_T
Cuts at: 0 1490 1863
Size: 1490 373
PflMI CCAn_nnn'nTGG
Cuts at: 0 622 1863
Size: 622 1241
PshAI GACnn'nnGTC
Cuts at: 0 22 1863
Size: 22 1841
PsiI TTA'TAA
Cuts at: 0 335 1863
Size: 335 1528
PstI C_TGCA'G
Cuts at: 0 954 1863
Size: 954 909
PvuII CAG'CTG
Cuts at: 0 751 1863
Size: 751 1112
SacI G_AGCT'C
Cuts at: 0 913 1343 1863
Size: 913 430 520
Fragments arranged by size:
913 520 430
SapI GCTCTTCn'nnn_
Cuts at: 0 1331 1748 1863
Size: 1331 417 115
Fragments arranged by size:
1331 417 115
ScaI AGT'ACT
Cuts at: 0 1402 1863
Size: 1402 461
SfcI C'TryA_G
Cuts at: 0 950 1071 1416 1751 1863
Size: 950 121 345 335 112
Fragments arranged by size:
950 345 335 121 112
SmlI C'TyrA_G
Cuts at: 0 1342 1863
Size: 1342 521
SspI AAT'ATT
Cuts at: 0 789 819 1863
Size: 789 30 1044
Fragments arranged by size:
1044 789 30
StyI C'CwwG_G
Cuts at: 0 20 1311 1522 1649 1863
Size: 20 1291 211 127 214
Fragments arranged by size:
1291 214 211 127 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 62 1237 1863
Size: 62 1175 626
Fragments arranged by size:
1175 626 62
TatI w'GTAC_w
Cuts at: 0 1400 1863
Size: 1400 463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1195 1227 1863
Size: 1195 32 636
Fragments arranged by size:
1195 636 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 425 1863
Size: 425 1438
XmnI GAAnn'nnTTC
Cuts at: 0 818 1229 1259 1863
Size: 818 411 30 604
Fragments arranged by size:
818 604 411 30
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApoI AvaI AvrII BanI
BanII BbsI BbvCI BciVI BfrBI BglII BmrI BplI
BpmI Bpu10I BpuEI BsaWI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BstAPI BstBI BstEII BstXI BstYI
BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI
FalI Hin4I MmeI MscI MspA1I NsiI NspI PasI
PciI PflMI PshAI PsiI PstI PvuII SacI SapI
ScaI SfcI SmlI SspI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BcgI BclI BglI BlpI Bme1580I
BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI
BssHII BssSI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoO109I EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI
XmaI ZraI