Restriction map for
pFN21AB3243
(Circular) (Six-base) MAPSORT of: pFN21AB3243.seq Check: 4398 from: 1 to: 7510
pFN21AB3243 7510 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2594 2594
Size: 7510
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5320 278
Size: 53 83 186 936 3784 2468
Fragments arranged by size:
3784 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4557 4853 1937
Size: 2620 296 4594
Fragments arranged by size:
4594 2620 296
Acc65I G'GTAC_C
Cuts at: 4831 4831
Size: 7510
AclI AA'CG_TT
Cuts at: 1563 5637 6010 1563
Size: 4074 373 3063
Fragments arranged by size:
4074 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2205 2358 3051 3113 4192 4572 5573
Size: 563 10 153 693 62 1079 380 1001
Cuts at: 5573 6957 7225 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1079 1001 693 563 380 268
153 62 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7313
AflIII A'CryG_T
Cuts at: 1203 6409 7386 1203
Size: 5206 977 1327
Fragments arranged by size:
5206 1327 977
AgeI A'CCGG_T
Cuts at: 6341 6341
Size: 7510
AhdI GACnn_n'nnGTC
Cuts at: 6239 6239
Size: 7510
AleI CACnn'nnGTG
Cuts at: 1166 3896 1166
Size: 2730 4780
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7510
AlwNI CAG_nnn'CTG
Cuts at: 2240 3883 4298 5253 6825 2240
Size: 1643 415 955 1572 2925
Fragments arranged by size:
2925 1643 1572 955 415
ApaI G_GGCC'C
Cuts at: 2542 2835 3251 2542
Size: 293 416 6801
Fragments arranged by size:
6801 416 293
ApaLI G'TGCA_C
Cuts at: 3831 4357 5567 6723 3831
Size: 526 1210 1156 4618
Fragments arranged by size:
4618 1210 1156 526
ApoI r'AATT_y
Cuts at: 1493 2323 2894 4251 4819 5045 5197 7306 1493
Size: 830 571 1357 568 226 152 2109 1697
Fragments arranged by size:
2109 1697 1357 830 571 568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6064 160
Size: 872 5032 1606
Fragments arranged by size:
5032 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7510
AvaI C'yCGr_G
Cuts at: 1870 1943 2363 3009 4835 1870
Size: 73 420 646 1826 4545
Fragments arranged by size:
4545 1826 646 420 73
BamHI G'GATC_C
Cuts at: 4840 5207 4840
Size: 367 7143
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2485 3197 3442 4385
Size: 325 217 627 221 477 712 245 943
Cuts at: 4385 4831 6286 618
Size: 446 1455 1842
Fragments arranged by size:
1842 1455 943 712 627 477 446 325
245 221 217
BanII G_rGCy'C
Cuts at: 1391 2542 2835 3251 3801 4114 4829 1391
Size: 1151 293 416 550 313 715 4072
Fragments arranged by size:
4072 1151 715 550 416 313 293
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 7510
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 3750 928
Size: 971 381 1470 4688
Fragments arranged by size:
4688 1470 971 381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5701 5735 1903
Size: 34 3764 34 3678
Fragments arranged by size:
3764 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3601 4282 5402 6612 3601
Size: 681 1120 1210 4499
Fragments arranged by size:
4499 1210 1120 681
BclI T'GATC_A
Cuts at: 1548 2346 1548
Size: 798 6712
BfrBI ATG'CAT
Cuts at: 2548 4277 2548
Size: 1729 5781
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6121 136
Size: 107 122 71 631 605 4449 1525
Fragments arranged by size:
4449 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2037 3814 4500 7505 2037
Size: 1777 686 3005 2042
Fragments arranged by size:
3005 2042 1777 686
BlpI GC'TnA_GC
Cuts at: 4927 4927
Size: 7510
Bme1580I G_kGCm'C
Cuts at: 1790 2542 2835 3202 3251 3835 3893 4361 5571
Size: 752 293 367 49 584 58 468 1210
Cuts at: 5571 6727 1790
Size: 1156 2573
Fragments arranged by size:
2573 1210 1156 752 584 468 367 293
58 49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7510
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3676 3770 4375
Size: 416 53 83 399 24 2259 94 605
Cuts at: 4375 4460 5241 6199 442
Size: 85 781 958 1753
Fragments arranged by size:
2259 1753 958 781 605 416 399 94
85 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7510
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2438
Size: 7510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2909 3879 3905 4208 6170 1239
Size: 1670 970 26 303 1962 2579
Fragments arranged by size:
2579 1962 1670 970 303 26
Bpu10I CC'TnA_GC
Cuts at: 4390 4401 4390
Size: 11 7499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2924 3956 4100 4442 5635 6500 6798 2924
Size: 1032 144 342 1193 865 298 3636
Fragments arranged by size:
3636 1193 1032 865 342 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6173 882
Size: 617 4674 2219
Fragments arranged by size:
4674 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3118 4084 5301 493
Size: 711 1914 966 1217 2702
Fragments arranged by size:
2702 1914 1217 966 711
BsaBI GATnn'nnATC
Cuts at: 2151 2195 7504 2151
Size: 44 5309 2157
Fragments arranged by size:
5309 2157 44
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 5317 5699 6334
Size: 53 83 186 936 476 3308 382 635
Cuts at: 6334 275
Size: 1451
Fragments arranged by size:
3308 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5943 6341 6615 6762 7173 5943
Size: 398 274 147 411 6280
Fragments arranged by size:
6280 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3786 3816 1178
Size: 30 165 30 2383 30 4872
Fragments arranged by size:
4872 2383 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 1186
Size: 1444 6066
BseYI C'CCAG_C
Cuts at: 2399 4430 6713 7209 2399
Size: 2031 2283 496 2700
Fragments arranged by size:
2700 2283 2031 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 1163
Size: 573 585 6352
Fragments arranged by size:
6352 585 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4960 5721 6749 1540
Size: 275 186 2959 761 1028 2301
Fragments arranged by size:
2959 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2864 3835 4361 4730 5571 5656 6727 2864
Size: 971 526 369 841 85 1071 3647
Fragments arranged by size:
3647 1071 971 841 526 369 85
BsmI GAATG_Cn'
Cuts at: 4277 4279 5021 5114 4277
Size: 2 742 93 6673
Fragments arranged by size:
6673 742 93 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2542 2835 2864 3202 3251 3801 3835
Size: 399 752 293 29 338 49 550 34
Cuts at: 3835 3893 4114 4361 4730 4829 5571 5656 6727
Size: 58 221 247 369 99 742 85 1071
Cuts at: 6727 1391
Size: 2174
Fragments arranged by size:
2174 1071 752 742 550 399 369 338
293 247 221 99 85 58 49 34
29
BspEI T'CCGG_A
Cuts at: 7173 7173
Size: 7510
BspHI T'CATG_A
Cuts at: 3400 5399 7129 3400
Size: 1999 1730 3781
Fragments arranged by size:
3781 1999 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2594 4865 844
Size: 1750 2271 3489
Fragments arranged by size:
3489 2271 1750
BsrBI CCG'CTC
Cuts at: 1394 1823 5397 1394
Size: 429 3574 3507
Fragments arranged by size:
3574 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2853 3951 4488 6005 6179 65
Size: 1183 1605 1098 537 1517 174 1396
Fragments arranged by size:
1605 1517 1396 1183 1098 537 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3099 4059 6154 6341 1700
Size: 110 1289 960 2095 187 2869
Fragments arranged by size:
2869 2095 1289 960 187 110
BsrGI T'GTAC_A
Cuts at: 96 2284 96
Size: 2188 5322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7510
BssSI C'ACGA_G
Cuts at: 5570 6582 5570
Size: 1012 6498
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2554 794
Size: 1760 5750
BstBI TT'CG_AA
Cuts at: 6314 6314
Size: 7510
BstEII G'GTnAC_C
Cuts at: 1184 2417 1184
Size: 1233 6277
BstXI CCAn_nnnn'nTGG
Cuts at: 2910 4594 2910
Size: 1684 5826
BstYI r'GATC_y
Cuts at: 1972 2037 3814 4500 4840 5207 5592 5609 7061
Size: 65 1777 686 340 367 385 17 1452
Cuts at: 7061 7147 7159 7505 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1777 1452 686 385 367 346 340
86 65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2451 3626 2451
Size: 1175 6335
BtgI C'CryG_G
Cuts at: 513 1065 2004 4137 513
Size: 552 939 2133 3886
Fragments arranged by size:
3886 2133 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3913 487
Size: 683 56 2687 4084
Fragments arranged by size:
4084 2687 683 56
BtsI GCAGTG_nn'
Cuts at: 3351 4143 5026 5820 5840 3351
Size: 792 883 794 20 5021
Fragments arranged by size:
5021 883 794 792 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7510
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7475
DraI TTT'AAA
Cuts at: 4814 4814
Size: 7510
DraIII CAC_nnn'GTG
Cuts at: 4087 4087
Size: 7510
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6517 809
Size: 507 5201 1802
Fragments arranged by size:
5201 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3378 3699 4266
Size: 54 1425 50 275 18 1548 321 567
Cuts at: 4266 4957 5846 7475 8
Size: 691 889 1629 43
Fragments arranged by size:
1629 1548 1425 889 691 567 321 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4957 1537
Size: 275 3145 4090
Fragments arranged by size:
4090 3145 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 3027 3306 3537 4164 5440 1353
Size: 828 846 279 231 627 1276 3423
Fragments arranged by size:
3423 1276 846 828 627 279 231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6097 6471 6617 387
Size: 1064 138 4508 374 146 1280
Fragments arranged by size:
4508 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2205 2358 2909 3051 3113 3879
Size: 393 563 10 153 551 142 62 766
Cuts at: 3879 3905 4192 4208 4572 5573 6170 6957 7225
Size: 26 287 16 364 1001 597 787 268
Cuts at: 7225 1239
Size: 1524
Fragments arranged by size:
1524 1001 787 766 597 563 551 393
364 287 268 153 142 62 26 16
10
EcoNI CCTnn'n_nnAGG
Cuts at: 4400 4400
Size: 7510
EcoO109I rG'GnC_Cy
Cuts at: 2538 2539 2831 3247 3248 2538
Size: 1 292 416 1 6800
Fragments arranged by size:
6800 416 292 1 1
EcoRI G'AATT_C
Cuts at: 2323 4251 4819 5197 2323
Size: 1928 568 378 4636
Fragments arranged by size:
4636 1928 568 378
EcoRV GAT'ATC
Cuts at: 3691 3691
Size: 7510
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 3445 4405 4588 2476
Size: 969 960 183 5398
Fragments arranged by size:
5398 969 960 183
FspI TGC'GCA
Cuts at: 6016 6016
Size: 7510
HaeII r_GCGC'y
Cuts at: 1678 2012 3276 6657 1678
Size: 334 1264 3381 2531
Fragments arranged by size:
3381 2531 1264 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2590 2622 3786 3818 4280
Size: 32 135 32 1018 32 1164 32 462
Cuts at: 4280 4312 6151 6183 6225 6257 1373
Size: 32 1839 32 42 32 2626
Fragments arranged by size:
2626 1839 1164 1018 462 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4854 5100 669
Size: 988 281 2916 246 3079
Fragments arranged by size:
3079 2916 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3974 748
Size: 3226 4284
HpaI GTT'AAC
Cuts at: 5100 5100
Size: 7510
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 7510
KpnI G_GTAC'C
Cuts at: 4835 4835
Size: 7510
MfeI C'AATT_G
Cuts at: 5109 5109
Size: 7510
MluI A'CGCG_T
Cuts at: 7386 7386
Size: 7510
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3164 3585 3677 6624 6808 1305
Size: 1859 421 92 2947 184 2007
Fragments arranged by size:
2947 2007 1859 421 184 92
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3701 10
Size: 54 1425 343 1869 3819
Fragments arranged by size:
3819 1869 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2610 2639 3305 3896 4031 4592 5468
Size: 648 1444 29 666 591 135 561 876
Cuts at: 5468 5827 5986 518
Size: 359 159 2042
Fragments arranged by size:
2042 1444 876 666 648 591 561 359
159 135 29
MspA1I CmG'CkG
Cuts at: 1606 4926 5603 6751 6996 1606
Size: 3320 677 1148 245 2120
Fragments arranged by size:
3320 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 7510
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6019
Fragments arranged by size:
6019 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7510
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7510
NotI GC'GGCC_GC
Cuts at: 4957 4957
Size: 7510
NruI TCG'CGA
Cuts at: 4671 4671
Size: 7510
NsiI A_TGCA'T
Cuts at: 2550 4279 2550
Size: 1729 5781
NspI r_CATG'y
Cuts at: 1132 1229 2507 4868 6413 1132
Size: 97 1278 2361 1545 2229
Fragments arranged by size:
2361 2229 1545 1278 97
PasI CC'CwG_GG
Cuts at: 1348 3148 1348
Size: 1800 5710
PciI A'CATG_T
Cuts at: 6409 6409
Size: 7510
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3591 4087 1108
Size: 2483 496 4531
Fragments arranged by size:
4531 2483 496
PfoI T'CCnGG_A
Cuts at: 2886 2886
Size: 7510
PmeI GTTT'AAAC
Cuts at: 4814 4814
Size: 7510
PmlI CAC'GTG
Cuts at: 4084 4084
Size: 7510
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5579 5611 7092 7124 5579
Size: 32 1481 32 5965
Fragments arranged by size:
5965 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7510
PsiI TTA'TAA
Cuts at: 5080 5080
Size: 7510
PspOMI G'GGCC_C
Cuts at: 2538 2831 3247 2538
Size: 293 416 6801
Fragments arranged by size:
6801 416 293
PstI C_TGCA'G
Cuts at: 830 3280 3358 4862 830
Size: 2450 78 1504 3478
Fragments arranged by size:
3478 2450 1504 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7510
SalI G'TCGA_C
Cuts at: 1936 4852 1936
Size: 2916 4594
SbfI CC_TGCA'GG
Cuts at: 4862 4862
Size: 7510
ScaI AGT'ACT
Cuts at: 5758 7470 5758
Size: 1712 5798
SexAI A'CCwGG_T
Cuts at: 2098 2098
Size: 7510
SfcI C'TryA_G
Cuts at: 826 1045 3276 3354 4647 4858 5993 6674 6865
Size: 219 2231 78 1293 211 1135 681 191
Cuts at: 6865 826
Size: 1471
Fragments arranged by size:
2231 1471 1293 1135 681 219 211 191
78
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 7510
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7510
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7510
SmaI CCC'GGG
Cuts at: 1872 4837 1872
Size: 2965 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2939 3009 3971 4115 4457 5614
Size: 197 926 996 70 962 144 342 1157
Cuts at: 5614 6515 6777 820
Size: 901 262 1553
Fragments arranged by size:
1553 1157 996 962 926 901 342 262
197 144 70
SnaBI TAC'GTA
Cuts at: 493 5301 493
Size: 4808 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7510
SphI G_CATG'C
Cuts at: 1132 2507 4868 1132
Size: 1375 2361 3774
Fragments arranged by size:
3774 2361 1375
SspI AAT'ATT
Cuts at: 5 52 2124 5434 5
Size: 47 2072 3310 2081
Fragments arranged by size:
3310 2081 2072 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7510
StyI C'CwwG_G
Cuts at: 513 1065 2004 3193 4164 4949 513
Size: 552 939 1189 971 785 3074
Fragments arranged by size:
3074 1189 971 939 785 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2753 4444 5537 5554 5707 5892 6337
Size: 1255 1173 1691 1093 17 153 185 445
Cuts at: 6337 325
Size: 1498
Fragments arranged by size:
1691 1498 1255 1173 1093 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2284 5756
Size: 274 80 33 51 1443 276 31 3472
Cuts at: 5756 7468 96
Size: 1712 138
Fragments arranged by size:
3472 1712 1443 276 274 138 80 51
33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2800 2832 7470 7502 2800
Size: 32 4638 32 2808
Fragments arranged by size:
4638 2808 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7510
XbaI T'CTAG_A
Cuts at: 3739 4846 3739
Size: 1107 6403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2884 3001 3709 2884
Size: 117 708 6685
Fragments arranged by size:
6685 708 117
XhoI C'TCGA_G
Cuts at: 1943 3009 1943
Size: 1066 6444
XmaI C'CCGG_G
Cuts at: 1870 4835 1870
Size: 2965 4545
XmnI GAAnn'nnTTC
Cuts at: 4277 4527 5639 4277
Size: 250 1112 6148
Fragments arranged by size:
6148 1112 250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5318 276
Size: 53 83 186 936 3784 2468
Fragments arranged by size:
3784 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BsiWI BsmBI
BstZ17I EcoICRI FseI FspAI NaeI NgoMIV PacI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI