Restriction map for
pFN21AB3241
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspI
AvaI AviII AvrII BamHI BbrPI
BbvCI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MluI
MroI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 2857 GTAGAC GT^[AC][GT]AC
AccII 999 CGCG CG^CG
AhdI 410 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 720 GTGCAC G^TGCAC
ApaLI 720 GTGCAC G^TGCAC
AspEI 410 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 729 TGGCCA TGG^CCA
BbeI 1025 GGCGCC GGCGC^C
BcgI 1946 GCATGGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1978 GGATAC GTATCC(6/5)
BclI 1428 TGATCA T^GATCA
BcnI 2627 CCCGG CC^[GC]GG
BsaWI 171 ACCGGA [AT]^CCGG[AT]
BsmBI 2721 GAGACG CGTCTC(1/5)
BsrBI 1858 CCGCTC CCGCTC(-3/-3)
BsrDI 799 CATTGC GCAATG(2/0)
BstAPI 2342 GCAGTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2831 TTCGAA TT^CGAA
BstUI 999 CGCG CG^CG
DrdI 922 GACAAGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 410 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2388 GAATTC G^AATTC
EcoRV 2152 GATATC GAT^ATC
FbaI 1428 TGATCA T^GATCA
HaeII 1025 GGCGCC [GA]GCGC^[CT]
KasI 1025 GGCGCC G^GCGCC
MfeI 160 CAATTG C^AATTG
MluNI 729 TGGCCA TGG^CCA
MscI 729 TGGCCA TGG^CCA
MunI 160 CAATTG C^AATTG
MvnI 999 CGCG CG^CG
NaeI 1028 GCCGGC GCC^GGC
NarI 1025 GGCGCC GG^CGCC
NciI 2627 CCCGG CC^[GC]GG
NcoI 390 CCATGG C^CATGG
NdeI 1937 CATATG CA^TATG
NgoMIV 1028 GCCGGC G^CCGGC
NheI 1038 GCTAGC G^CTAGC
NspV 2831 TTCGAA TT^CGAA
PpuMI 2837 AGGACCC [GA]G^G[AT]CC[CT]
SapI 463 GAAGAGC GCTCTTC(1/4)
ScaI 2595 AGTACT AGT^ACT
SexAI 2517 ACCAGGT A^CC[AT]GGT
SfoI 1025 GGCGCC GGC^GCC
SfuI 2831 TTCGAA TT^CGAA
SmlI 1797 CTTGAG C^T[CT][GA]AG
SphI 1744 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 1348 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1541 CAGAGTCTG
Asp700I 847 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1403 GAATACATTC
AvaII 387 GGTCC G^G[AT]CC
2838 GGACC
BanI 360 GGCACC G^G[CT][GA]CC
1025 GGCGCC
BbsI 1657 GAAGAC GAAGAC(2/6)
2056 GAAGAC
BpmI 2707 CTCCAG CTGGAG(16/14)
115 CTGGAG
BpuAI 1657 GAAGAC GAAGAC(2/6)
2056 GAAGAC
BsaBI 2187 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2782 GATGCCAATC
BsaI 2514 GAGACC GGTCTC(1/5)
516 GGTCTC
BseRI 66 CTCCTC GAGGAG(10/8)
1579 CTCCTC
BsgI 782 GTGCAG GTGCAG(16/14)
1121 GTGCAG
BsrFI 1028 GCCGGC [GA]^CCGG[CT]
2447 ACCGGC
CfoI 1026 GCGC GCG^C
1944 GCGC
Cfr10I 1028 GCCGGC [GA]^CCGG[CT]
2447 ACCGGC
DraI 300 TTTAAA TTT^AAA
2767 TTTAAA
DraII 1720 GGGGCCT [GA]G^G[ACGT]CC[CT]
2837 AGGACCC
DsaI 390 CCATGG C^C[GA][CT]GG
676 CCGTGG
EaeI 729 TGGCCA [CT]^GGCC[GA]
2635 CGGCCA
EcoO109I 1720 GGGGCCT [GA]G^G[ACGT]CC[CT]
2837 AGGACCC
EcoT22I 509 ATGCAT ATGCA^T
2046 ATGCAT
FauI 997 CCCGC CCCGC(4/6)
927 GCGGG
HgaI 2623 GACGC GACGC(5/10)
1737 GCGTC
HhaI 1026 GCGC GCG^C
1944 GCGC
HinP1I 1026 GCGC G^CGC
1944 GCGC
MaeII 604 ACGT A^CGT
718 ACGT
MamI 2187 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2782 GATGCCAATC
NsiI 509 ATGCAT ATGCA^T
2046 ATGCAT
NspI 887 ACATGC [GA]CATG^[CT]
1744 GCATGC
SacI 1903 GAGCTC GAGCT^C
2575 GAGCTC
SspI 1447 AATATT AAT^ATT
1681 AATATT
XmnI 847 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1403 GAATACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI BanII BbvI
BfaI BglII BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII