(Circular) (Six-base) MAPSORT of: pFN21AB3241.seq Check: 2764 from: 1 to: 7582 pFN21AB3241 7582 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5392 278 Size: 53 83 186 936 3856 2468 Fragments arranged by size: 3856 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4863 4925 1937 Size: 2926 62 4594 Fragments arranged by size: 4594 2926 62 Acc65I G'GTAC_C Cuts at: 4903 4903 Size: 7582 AclI AA'CG_TT Cuts at: 1563 5709 6082 1563 Size: 4146 373 3063 Fragments arranged by size: 4146 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2306 2843 3308 3600 3681 5645 7029 7297 Size: 674 537 465 292 81 1964 1384 268 Cuts at: 7297 1632 Size: 1917 Fragments arranged by size: 1964 1917 1384 674 537 465 292 268 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7385 AflIII A'CryG_T Cuts at: 1203 6481 7458 1203 Size: 5278 977 1327 Fragments arranged by size: 5278 1327 977 AgeI A'CCGG_T Cuts at: 6413 6413 Size: 7582 AhdI GACnn_n'nnGTC Cuts at: 2420 6311 2420 Size: 3891 3691 AleI CACnn'nnGTG Cuts at: 1166 3885 1166 Size: 2719 4863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2855 1184 Size: 1671 5911 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4510 4542 4510 Size: 32 7550 AlwNI CAG_nnn'CTG Cuts at: 3358 3551 5325 6897 3358 Size: 193 1774 1572 4043 Fragments arranged by size: 4043 1774 1572 193 ApaLI G'TGCA_C Cuts at: 2725 5639 6795 2725 Size: 2914 1156 3512 Fragments arranged by size: 3512 2914 1156 ApoI r'AATT_y Cuts at: 1493 2845 3137 3209 4138 4393 4669 4766 4891 Size: 1352 292 72 929 255 276 97 125 Cuts at: 4891 5117 5269 7378 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 929 292 276 255 226 152 125 97 72 AseI AT'TA_AT Cuts at: 160 1032 6136 160 Size: 872 5104 1606 Fragments arranged by size: 5104 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7582 AvaI C'yCGr_G Cuts at: 1870 1943 4907 1870 Size: 73 2964 4545 Fragments arranged by size: 4545 2964 73 BamHI G'GATC_C Cuts at: 4912 5279 4912 Size: 367 7215 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2365 3030 4903 6358 618 Size: 325 217 627 578 665 1873 1455 1842 Fragments arranged by size: 1873 1842 1455 665 627 578 325 217 BanII G_rGCy'C Cuts at: 1391 2151 3912 4584 4901 1391 Size: 760 1761 672 317 4072 Fragments arranged by size: 4072 1761 760 672 317 BbeI G_GCGC'C Cuts at: 3034 3034 Size: 7582 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3669 4068 928 Size: 971 1770 399 4442 Fragments arranged by size: 4442 1770 971 399 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 5773 5807 1903 Size: 34 2003 34 1799 34 3678 Fragments arranged by size: 3678 2003 1799 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5474 6684 3977 Size: 1497 1210 4875 Fragments arranged by size: 4875 1497 1210 BclI T'GATC_A Cuts at: 1548 3433 1548 Size: 1885 5697 BfrBI ATG'CAT Cuts at: 2516 4053 2516 Size: 1537 6045 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6193 136 Size: 107 122 71 631 605 4521 1525 Fragments arranged by size: 4521 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2794 4609 4718 7577 2794 Size: 1815 109 2859 2799 Fragments arranged by size: 2859 2799 1815 109 BlpI GC'TnA_GC Cuts at: 4999 4999 Size: 7582 Bme1580I G_kGCm'C Cuts at: 1790 2729 5643 6799 1790 Size: 939 2914 1156 2573 Fragments arranged by size: 2914 2573 1156 939 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7582 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4022 4837 5313 Size: 416 53 83 399 24 2605 815 476 Cuts at: 5313 6271 442 Size: 958 1753 Fragments arranged by size: 2605 1753 958 815 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3047 1056 Size: 1991 5591 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 4697 6242 1239 Size: 902 2556 1545 2579 Fragments arranged by size: 2579 2556 1545 902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 5707 6572 6870 3823 Size: 1884 865 298 4535 Fragments arranged by size: 4535 1884 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2527 4513 6245 882 Size: 617 1028 1986 1732 2219 Fragments arranged by size: 2219 1986 1732 1028 617 BsaAI yAC'GTr Cuts at: 493 1204 5373 493 Size: 711 4169 2702 Fragments arranged by size: 4169 2702 711 BsaBI GATnn'nnATC Cuts at: 4196 4791 7576 4196 Size: 595 2785 4202 Fragments arranged by size: 4202 2785 595 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3031 3742 4629 5389 Size: 53 83 186 936 1498 711 887 760 Cuts at: 5389 5771 6406 275 Size: 382 635 1451 Fragments arranged by size: 1498 1451 936 887 760 711 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2176 6015 6413 6687 6834 7245 2176 Size: 3839 398 274 147 411 2513 Fragments arranged by size: 3839 2513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2035 2065 4510 4540 1178 Size: 30 165 30 632 30 2445 30 4220 Fragments arranged by size: 4220 2445 632 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2062 3575 1186 Size: 876 1513 5193 Fragments arranged by size: 5193 1513 876 BseYI C'CCAG_C Cuts at: 2211 6785 7281 2211 Size: 4574 496 2512 Fragments arranged by size: 4574 2512 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2808 3147 1163 Size: 573 1072 339 5598 Fragments arranged by size: 5598 1072 573 339 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5032 5793 6821 1540 Size: 275 186 3031 761 1028 2301 Fragments arranged by size: 3031 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2475 2729 3585 3912 4584 5643 5728 6799 2475 Size: 254 856 327 672 1059 85 1071 3258 Fragments arranged by size: 3258 1071 1059 856 672 327 254 85 BsmI GAATG_Cn' Cuts at: 3103 3235 3385 4055 5093 5186 3103 Size: 132 150 670 1038 93 5499 Fragments arranged by size: 5499 1038 670 150 132 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4720 4720 Size: 7582 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2475 2729 3585 3912 4584 4901 Size: 399 361 324 254 856 327 672 317 Cuts at: 4901 5643 5728 6799 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 856 742 672 399 361 327 324 317 254 85 BspEI T'CCGG_A Cuts at: 7245 7245 Size: 7582 BspHI T'CATG_A Cuts at: 5471 7201 5471 Size: 1730 5852 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4937 844 Size: 4093 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3865 5469 1394 Size: 429 2042 1604 3507 Fragments arranged by size: 3507 2042 1604 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2803 6077 6251 65 Size: 1183 1555 3274 174 1396 Fragments arranged by size: 3274 1555 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3033 4452 6226 6413 1700 Size: 110 1223 1419 1774 187 2869 Fragments arranged by size: 2869 1774 1419 1223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7582 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7582 BssSI C'ACGA_G Cuts at: 5642 6654 5642 Size: 1012 6570 BstAPI GCAn_nnn'nTGC Cuts at: 794 4353 794 Size: 3559 4023 BstBI TT'CG_AA Cuts at: 4837 6386 4837 Size: 1549 6033 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7582 BstYI r'GATC_y Cuts at: 1972 2794 4594 4609 4718 4912 5279 5664 5681 Size: 822 1800 15 109 194 367 385 17 Cuts at: 5681 7133 7219 7231 7577 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1800 1452 822 385 367 346 194 109 86 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2395 2681 513 Size: 552 1330 286 5414 Fragments arranged by size: 5414 1330 552 286 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6843 Fragments arranged by size: 6843 683 56 BtsI GCAGTG_nn' Cuts at: 3060 4354 5098 5892 5912 3060 Size: 1294 744 794 20 4730 Fragments arranged by size: 4730 1294 794 744 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7582 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2802 2837 529 Size: 35 2238 35 5274 Fragments arranged by size: 5274 2238 35 35 DraI TTT'AAA Cuts at: 2307 4774 4886 2307 Size: 2467 112 5003 Fragments arranged by size: 5003 2467 112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2933 6589 809 Size: 507 1617 3656 1802 Fragments arranged by size: 3656 1802 1617 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2734 4640 5029 Size: 54 1425 50 275 18 904 1906 389 Cuts at: 5029 5918 7547 8 Size: 889 1629 43 Fragments arranged by size: 1906 1629 1425 904 889 389 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5029 1537 Size: 275 3217 4090 Fragments arranged by size: 4090 3217 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2080 2463 2766 5512 1353 Size: 727 383 303 2746 3423 Fragments arranged by size: 3423 2746 727 383 303 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6169 6543 6689 387 Size: 1064 138 4580 374 146 1280 Fragments arranged by size: 4580 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3910 4582 3910 Size: 672 6910 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2306 2843 3308 3600 3681 4697 Size: 393 509 165 537 465 292 81 1016 Cuts at: 4697 5645 6242 7029 7297 1239 Size: 948 597 787 268 1524 Fragments arranged by size: 1524 1016 948 787 597 537 509 465 393 292 268 165 81 EcoNI CCTnn'n_nnAGG Cuts at: 2089 2195 2307 3887 3917 2089 Size: 106 112 1580 30 5754 Fragments arranged by size: 5754 1580 112 106 30 EcoO109I rG'GnC_Cy Cuts at: 3726 4843 3726 Size: 1117 6465 EcoRI G'AATT_C Cuts at: 4393 4891 5269 4393 Size: 498 378 6706 Fragments arranged by size: 6706 498 378 EcoRV GAT'ATC Cuts at: 4159 4159 Size: 7582 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4631 4631 Size: 7582 FspI TGC'GCA Cuts at: 6088 6088 Size: 7582 HaeII r_GCGC'y Cuts at: 1678 3034 6729 1678 Size: 1356 3695 2531 Fragments arranged by size: 3695 2531 1356 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2406 2438 3611 3641 3643 Size: 32 135 32 834 32 1173 30 2 Cuts at: 3643 3673 6223 6255 6297 6329 1373 Size: 30 2550 32 42 32 2626 Fragments arranged by size: 2626 2550 1173 834 135 42 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 4926 5172 669 Size: 988 281 2988 246 3079 Fragments arranged by size: 3079 2988 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7582 HpaI GTT'AAC Cuts at: 5172 5172 Size: 7582 KasI G'GCGC_C Cuts at: 3030 3030 Size: 7582 KpnI G_GTAC'C Cuts at: 4907 4907 Size: 7582 MfeI C'AATT_G Cuts at: 2165 5181 2165 Size: 3016 4566 MluI A'CGCG_T Cuts at: 7458 7458 Size: 7582 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2078 3135 3373 4189 6696 6880 1305 Size: 773 1057 238 816 2507 184 2007 Fragments arranged by size: 2507 2007 1057 816 773 238 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2736 10 Size: 54 1425 343 904 4856 Fragments arranged by size: 4856 1425 904 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2408 3335 3885 5540 5899 6058 Size: 648 898 344 927 550 1655 359 159 Cuts at: 6058 518 Size: 2042 Fragments arranged by size: 2042 1655 927 898 648 550 359 344 159 MspA1I CmG'CkG Cuts at: 1606 2211 2618 2633 4998 5675 6823 7068 1606 Size: 605 407 15 2365 677 1148 245 2120 Fragments arranged by size: 2365 2120 1148 677 605 407 245 15 NaeI GCC'GGC Cuts at: 3035 3035 Size: 7582 NarI GG'CG_CC Cuts at: 3031 3031 Size: 7582 NcoI C'CATG_G Cuts at: 513 1065 2395 513 Size: 552 1330 5700 Fragments arranged by size: 5700 1330 552 NdeI CA'TA_TG Cuts at: 387 3943 387 Size: 3556 4026 NgoMIV G'CCGG_C Cuts at: 3033 3033 Size: 7582 NheI G'CTAG_C Cuts at: 1052 3043 1052 Size: 1991 5591 NotI GC'GGCC_GC Cuts at: 5029 5029 Size: 7582 NsiI A_TGCA'T Cuts at: 2518 4055 2518 Size: 1537 6045 NspI r_CATG'y Cuts at: 1132 1229 2896 3753 4940 6485 1132 Size: 97 1667 857 1187 1545 2229 Fragments arranged by size: 2229 1667 1545 1187 857 97 PasI CC'CwG_GG Cuts at: 1348 4847 1348 Size: 3499 4083 PciI A'CATG_T Cuts at: 6481 6481 Size: 7582 PflMI CCAn_nnn'nTGG Cuts at: 1108 4034 4238 4420 1108 Size: 2926 204 182 4270 Fragments arranged by size: 4270 2926 204 182 PfoI T'CCnGG_A Cuts at: 2334 3912 2334 Size: 1578 6004 PmeI GTTT'AAAC Cuts at: 4886 4886 Size: 7582 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4510 4542 5651 5683 7164 7196 4510 Size: 32 1109 32 1481 32 4896 Fragments arranged by size: 4896 1481 1109 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4843 4843 Size: 7582 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7582 PsiI TTA'TAA Cuts at: 5152 5152 Size: 7582 PstI C_TGCA'G Cuts at: 830 2991 3879 4684 4934 830 Size: 2161 888 805 250 3478 Fragments arranged by size: 3478 2161 888 805 250 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7582 PvuII CAG'CTG Cuts at: 2211 2618 2633 2211 Size: 407 15 7160 Fragments arranged by size: 7160 407 15 SacI G_AGCT'C Cuts at: 3912 4584 3912 Size: 672 6910 SalI G'TCGA_C Cuts at: 1936 4924 1936 Size: 2988 4594 SapI GCTCTTCn'nnn_ Cuts at: 2463 2463 Size: 7582 SbfI CC_TGCA'GG Cuts at: 4934 4934 Size: 7582 ScaI AGT'ACT Cuts at: 4602 5830 7542 4602 Size: 1228 1712 4642 Fragments arranged by size: 4642 1712 1228 SexAI A'CCwGG_T Cuts at: 4522 4522 Size: 7582 SfcI C'TryA_G Cuts at: 826 1045 2987 3270 3875 4680 4930 6065 6746 Size: 219 1942 283 605 805 250 1135 681 Cuts at: 6746 6937 826 Size: 191 1471 Fragments arranged by size: 1942 1471 1135 805 681 605 283 250 219 191 SfoI GGC'GCC Cuts at: 3032 3032 Size: 7582 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7582 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7582 SmaI CCC'GGG Cuts at: 1872 4909 1872 Size: 3037 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 5686 6587 6849 820 Size: 197 926 1859 1884 901 262 1553 Fragments arranged by size: 1884 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5373 493 Size: 4880 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7582 SphI G_CATG'C Cuts at: 1132 3753 4940 1132 Size: 2621 1187 3774 Fragments arranged by size: 3774 2621 1187 SspI AAT'ATT Cuts at: 5 52 3454 3688 5506 5 Size: 47 3402 234 1818 2081 Fragments arranged by size: 3402 2081 1818 234 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7582 StyI C'CwwG_G Cuts at: 513 1065 2196 2395 4033 5021 513 Size: 552 1131 199 1638 988 3074 Fragments arranged by size: 3074 1638 1131 988 552 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4123 5609 5626 5779 5964 6409 325 Size: 1255 2543 1486 17 153 185 445 1498 Fragments arranged by size: 2543 1498 1486 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4551 4600 5828 Size: 274 80 33 51 1443 2574 49 1228 Cuts at: 5828 7540 96 Size: 1712 138 Fragments arranged by size: 2574 1712 1443 1228 274 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7542 7574 7542 Size: 32 7550 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7582 XbaI T'CTAG_A Cuts at: 4918 4918 Size: 7582 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7582 XmaI C'CCGG_G Cuts at: 1870 4907 1870 Size: 3037 4545 XmnI GAAnn'nnTTC Cuts at: 2856 3412 5711 2856 Size: 556 2299 4727 Fragments arranged by size: 4727 2299 556 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5390 276 Size: 53 83 186 936 3856 2468 Fragments arranged by size: 3856 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI