Restriction map for
pFN21AB3241
(Linear) (Six-base) MAPSORT of: KIBB3241.seq Check: 5186 from: 1 to: 2877
KIBB3241.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 2858 2877
Size: 2858 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 838 1303 1595 1676 2877
Size: 301 537 465 292 81 1201
Fragments arranged by size:
1201 537 465 301 292 81
AhdI GACnn_n'nnGTC
Cuts at: 0 415 2877
Size: 415 2462
AleI CACnn'nnGTG
Cuts at: 0 1880 2877
Size: 1880 997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 850 2877
Size: 850 2027
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2505 2537 2877
Size: 2505 32 340
Fragments arranged by size:
2505 340 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1353 1546 2877
Size: 1353 193 1331
Fragments arranged by size:
1353 1331 193
ApaLI G'TGCA_C
Cuts at: 0 720 2877
Size: 720 2157
ApoI r'AATT_y
Cuts at: 0 840 1132 1204 2133 2388 2664 2761 2877
Size: 840 292 72 929 255 276 97 116
Fragments arranged by size:
929 840 292 276 255 116 97 72
BanI G'GyrC_C
Cuts at: 0 360 1025 2877
Size: 360 665 1852
Fragments arranged by size:
1852 665 360
BanII G_rGCy'C
Cuts at: 0 146 1907 2579 2877
Size: 146 1761 672 298
Fragments arranged by size:
1761 672 298 146
BbeI G_GCGC'C
Cuts at: 0 1029 2877
Size: 1029 1848
BbsI GAAGACnn'nnnn_
Cuts at: 0 1664 2063 2877
Size: 1664 399 814
Fragments arranged by size:
1664 814 399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1935 1969 2877
Size: 1935 34 908
Fragments arranged by size:
1935 908 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1972 2877
Size: 1972 905
BclI T'GATC_A
Cuts at: 0 1428 2877
Size: 1428 1449
BfrBI ATG'CAT
Cuts at: 0 511 2048 2877
Size: 511 1537 829
Fragments arranged by size:
1537 829 511
BglII A'GATC_T
Cuts at: 0 789 2604 2713 2877
Size: 789 1815 109 164
Fragments arranged by size:
1815 789 164 109
Bme1580I G_kGCm'C
Cuts at: 0 724 2877
Size: 724 2153
BmrI ACTGGGnnnn_n'
Cuts at: 0 2017 2832 2877
Size: 2017 815 45
Fragments arranged by size:
2017 815 45
BmtI G_CTAG'C
Cuts at: 0 1042 2877
Size: 1042 1835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 2692 2877
Size: 136 2556 185
Fragments arranged by size:
2556 185 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1818 2877
Size: 1818 1059
BsaI GGTCTCn'nnnn_
Cuts at: 0 522 2508 2877
Size: 522 1986 369
Fragments arranged by size:
1986 522 369
BsaBI GATnn'nnATC
Cuts at: 0 2191 2786 2877
Size: 2191 595 91
Fragments arranged by size:
2191 595 91
BsaHI Gr'CG_yC
Cuts at: 0 1026 1737 2624 2877
Size: 1026 711 887 253
Fragments arranged by size:
1026 887 711 253
BsaWI w'CCGG_w
Cuts at: 0 171 2877
Size: 171 2706
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 30 60 2505 2535 2877
Size: 30 30 2445 30 342
Fragments arranged by size:
2445 342 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 1570 2877
Size: 57 1513 1307
Fragments arranged by size:
1513 1307 57
BseYI C'CCAG_C
Cuts at: 0 206 2877
Size: 206 2671
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1142 2877
Size: 803 339 1735
Fragments arranged by size:
1735 803 339
BsiHKAI G_wGCw'C
Cuts at: 0 470 724 1580 1907 2579 2877
Size: 470 254 856 327 672 298
Fragments arranged by size:
856 672 470 327 298 254
BsmI GAATG_Cn'
Cuts at: 0 1098 1230 1380 2050 2877
Size: 1098 132 150 670 827
Fragments arranged by size:
1098 827 670 150 132
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2715 2877
Size: 2715 162
Bsp1286I G_dGCh'C
Cuts at: 0 146 470 724 1580 1907 2579 2877
Size: 146 324 254 856 327 672 298
Fragments arranged by size:
856 672 327 324 298 254 146
BsrBI CCG'CTC
Cuts at: 0 1860 2877
Size: 1860 1017
BsrDI GCAATG_nn'
Cuts at: 0 798 2877
Size: 798 2079
BsrFI r'CCGG_y
Cuts at: 0 1028 2447 2877
Size: 1028 1419 430
Fragments arranged by size:
1419 1028 430
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2348 2877
Size: 2348 529
BstBI TT'CG_AA
Cuts at: 0 2832 2877
Size: 2832 45
BstYI r'GATC_y
Cuts at: 0 789 2589 2604 2713 2877
Size: 789 1800 15 109 164
Fragments arranged by size:
1800 789 164 109 15
BtgI C'CryG_G
Cuts at: 0 390 676 2877
Size: 390 286 2201
Fragments arranged by size:
2201 390 286
BtsI GCAGTG_nn'
Cuts at: 0 1055 2349 2877
Size: 1055 1294 528
Fragments arranged by size:
1294 1055 528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 797 832 2877
Size: 797 35 2045
Fragments arranged by size:
2045 797 35
DraI TTT'AAA
Cuts at: 0 302 2769 2877
Size: 302 2467 108
Fragments arranged by size:
2467 302 108
DrdI GACnn_nn'nnGTC
Cuts at: 0 928 2877
Size: 928 1949
EaeI y'GGCC_r
Cuts at: 0 729 2635 2877
Size: 729 1906 242
Fragments arranged by size:
1906 729 242
EarI CTCTTCn'nnn_
Cuts at: 0 75 458 761 2877
Size: 75 383 303 2116
Fragments arranged by size:
2116 383 303 75
EcoICRI GAG'CTC
Cuts at: 0 1905 2577 2877
Size: 1905 672 300
Fragments arranged by size:
1905 672 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 301 838 1303 1595 1676 2692 2877
Size: 136 165 537 465 292 81 1016 185
Fragments arranged by size:
1016 537 465 292 185 165 136 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 84 190 302 1882 1912 2877
Size: 84 106 112 1580 30 965
Fragments arranged by size:
1580 965 112 106 84 30
EcoO109I rG'GnC_Cy
Cuts at: 0 1721 2838 2877
Size: 1721 1117 39
Fragments arranged by size:
1721 1117 39
EcoRI G'AATT_C
Cuts at: 0 2388 2877
Size: 2388 489
EcoRV GAT'ATC
Cuts at: 0 2154 2877
Size: 2154 723
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2626 2877
Size: 2626 251
HaeII r_GCGC'y
Cuts at: 0 1029 2877
Size: 1029 1848
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 401 433 1606 1636 1638 1668 2877
Size: 401 32 1173 30 2 30 1209
Fragments arranged by size:
1209 1173 401 32 30 30 2
KasI G'GCGC_C
Cuts at: 0 1025 2877
Size: 1025 1852
MfeI C'AATT_G
Cuts at: 0 160 2877
Size: 160 2717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1130 1368 2184 2877
Size: 73 1057 238 816 693
Fragments arranged by size:
1057 816 693 238 73
MscI TGG'CCA
Cuts at: 0 731 2877
Size: 731 2146
MslI CAynn'nnrTG
Cuts at: 0 59 403 1330 1880 2877
Size: 59 344 927 550 997
Fragments arranged by size:
997 927 550 344 59
MspA1I CmG'CkG
Cuts at: 0 206 613 628 2877
Size: 206 407 15 2249
Fragments arranged by size:
2249 407 206 15
NaeI GCC'GGC
Cuts at: 0 1030 2877
Size: 1030 1847
NarI GG'CG_CC
Cuts at: 0 1026 2877
Size: 1026 1851
NcoI C'CATG_G
Cuts at: 0 390 2877
Size: 390 2487
NdeI CA'TA_TG
Cuts at: 0 1938 2877
Size: 1938 939
NgoMIV G'CCGG_C
Cuts at: 0 1028 2877
Size: 1028 1849
NheI G'CTAG_C
Cuts at: 0 1038 2877
Size: 1038 1839
NsiI A_TGCA'T
Cuts at: 0 513 2050 2877
Size: 513 1537 827
Fragments arranged by size:
1537 827 513
NspI r_CATG'y
Cuts at: 0 891 1748 2877
Size: 891 857 1129
Fragments arranged by size:
1129 891 857
PasI CC'CwG_GG
Cuts at: 0 2842 2877
Size: 2842 35
PflMI CCAn_nnn'nTGG
Cuts at: 0 2029 2233 2415 2877
Size: 2029 204 182 462
Fragments arranged by size:
2029 462 204 182
PfoI T'CCnGG_A
Cuts at: 0 329 1907 2877
Size: 329 1578 970
Fragments arranged by size:
1578 970 329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2505 2537 2877
Size: 2505 32 340
Fragments arranged by size:
2505 340 32
PpuMI rG'GwC_Cy
Cuts at: 0 2838 2877
Size: 2838 39
PstI C_TGCA'G
Cuts at: 0 986 1874 2679 2877
Size: 986 888 805 198
Fragments arranged by size:
986 888 805 198
PvuII CAG'CTG
Cuts at: 0 206 613 628 2877
Size: 206 407 15 2249
Fragments arranged by size:
2249 407 206 15
SacI G_AGCT'C
Cuts at: 0 1907 2579 2877
Size: 1907 672 298
Fragments arranged by size:
1907 672 298
SapI GCTCTTCn'nnn_
Cuts at: 0 458 2877
Size: 458 2419
ScaI AGT'ACT
Cuts at: 0 2597 2877
Size: 2597 280
SexAI A'CCwGG_T
Cuts at: 0 2517 2877
Size: 2517 360
SfcI C'TryA_G
Cuts at: 0 982 1265 1870 2675 2877
Size: 982 283 605 805 202
Fragments arranged by size:
982 805 605 283 202
SfoI GGC'GCC
Cuts at: 0 1027 2877
Size: 1027 1850
SmlI C'TyrA_G
Cuts at: 0 1797 2877
Size: 1797 1080
SphI G_CATG'C
Cuts at: 0 1748 2877
Size: 1748 1129
SspI AAT'ATT
Cuts at: 0 1449 1683 2877
Size: 1449 234 1194
Fragments arranged by size:
1449 1194 234
StyI C'CwwG_G
Cuts at: 0 191 390 2028 2877
Size: 191 199 1638 849
Fragments arranged by size:
1638 849 199 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2118 2877
Size: 2118 759
TatI w'GTAC_w
Cuts at: 0 2546 2595 2877
Size: 2546 49 282
Fragments arranged by size:
2546 282 49
XmnI GAAnn'nnTTC
Cuts at: 0 851 1407 2877
Size: 851 556 1470
Fragments arranged by size:
1470 851 556
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII Bme1580I BmrI BmtI BpmI BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstAPI BstBI
BstYI BtgI BtsI CspCI DraI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PflMI PfoI PpiI PpuMI PstI PvuII SacI SapI
ScaI SexAI SfcI SfoI SmlI SphI SspI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbvCI BglI BlpI BmgBI BplI Bpu10I BsaAI BsiEI
BsiWI BspEI BspHI BspMI BsrGI BssHII BssSI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI DraIII EagI EciI
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
NotI NruI PacI PciI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI