Restriction map for
pFN21AB3223
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI Alw44I AlwNI AocI ApaI
ApaLI AscI AspHI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoO65I EcoT14I
EcoT22I FseI FspI HapII HgaI
Hin1I HindIII HpaI HpaII KasI
KspI MamI MroI MspA1I MspI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 1114 GGTACC G^GTACC
AflII 2008 CTTAAG C^TTAAG
AhdI 1681 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 519 AGCGCT AGC^GCT
AseI 1849 ATTAAT AT^TAAT
AsnI 1849 ATTAAT AT^TAAT
Asp700I 1651 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1114 GGTACC G^GTACC
AspEI 1681 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1126 TGGCCA TGG^CCA
BclI 249 TGATCA T^GATCA
BfrI 2008 CTTAAG C^TTAAG
BglI 2038 GCCGTATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 408 AGATCT A^GATCT
BlpI 48 GCTGAGC GC^T[ACGT]AGC
BpmI 1136 CTGGAG CTGGAG(16/14)
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
BsaI 498 GAGACC GGTCTC(1/5)
Bsp1407I 356 TGTACA T^GTACA
BspLU11I 776 ACATGT A^CATGT
BsrDI 1231 GCAATG GCAATG(2/0)
BsrGI 356 TGTACA T^GTACA
BstXI 2099 CCAACCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 408 AGATCT [GA]^GATC[CT]
CelII 48 GCTGAGC GC^T[ACGT]AGC
CfoI 520 GCGC GCG^C
DsaI 1940 CCGTGG C^C[GA][CT]GG
Eam1105I 1681 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1896 GAAGAG CTCTTC(1/4)
Eco47III 519 AGCGCT AGC^GCT
EcoNI 1105 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 752 GAATTC G^AATTC
EcoRV 1714 GATATC GAT^ATC
FauI 1842 GCGGG CCCGC(4/6)
FbaI 249 TGATCA T^GATCA
HaeII 519 AGCGCT [GA]GCGC^[CT]
HhaI 520 GCGC GCG^C
HinP1I 520 GCGC G^CGC
HincII 191 GTCAAC GT[CT]^[GA]AC
HindII 191 GTCAAC GT[CT]^[GA]AC
KpnI 1114 GGTACC GGTAC^C
Ksp632I 1896 GAAGAG CTCTTC(1/4)
MaeII 920 ACGT A^CGT
MfeI 1400 CAATTG C^AATTG
MflI 408 AGATCT [GA]^GATC[CT]
MluI 1836 ACGCGT A^CGCGT
MluNI 1126 TGGCCA TGG^CCA
MscI 1126 TGGCCA TGG^CCA
MunI 1400 CAATTG C^AATTG
NdeI 2113 CATATG CA^TATG
NheI 664 GCTAGC G^CTAGC
NspI 776 ACATGT [GA]CATG^[CT]
PflFI 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1849 ATTAAT AT^TAAT
PstI 515 CTGCAG CTGCA^G
ScaI 1747 AGTACT AGT^ACT
SspBI 356 TGTACA T^GTACA
Tth111I 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 735 CCAGCCTGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 408 AGATCT [GA]^GATC[CT]
XmnI 1651 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 895 CGCG CG^CG
1837 CGCG
AcsI 597 AAATTC [GA]^AATT[CT]
752 GAATTC
AflIII 776 ACATGT A^C[GA][CT]GT
1836 ACGCGT
ApoI 597 AAATTC [GA]^AATT[CT]
752 GAATTC
BanI 555 GGTGCC G^G[CT][GA]CC
1114 GGTACC
BanII 275 GAGCCC G[GA]GC[CT]^C
423 GAGCCC
BbsI 1483 GAAGAC GAAGAC(2/6)
869 GTCTTC
BmyI 275 GAGCCC G[AGT]GC[ACT]^C
423 GAGCCC
BpuAI 1483 GAAGAC GAAGAC(2/6)
869 GTCTTC
Bsp1286I 275 GAGCCC G[AGT]GC[ACT]^C
423 GAGCCC
BspHI 305 TCATGA T^CATGA
1659 TCATGA
BssSI 864 CACGAG CACGAG(-5/-1)
1213 CACGAG
BstUI 895 CGCG CG^CG
1837 CGCG
MvnI 895 CGCG CG^CG
1837 CGCG
RcaI 305 TCATGA T^CATGA
1659 TCATGA
SfcI 29 CTATAG C^T[GA][CT]AG
515 CTGCAG
SmlI 822 CTTGAG C^T[CT][GA]AG
2008 CTTAAG
Tsp45I 1355 GTCAC ^GT[GC]AC
1531 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XbaI