(Circular) (Six-base) MAPSORT of: pFN21AB3223.seq Check: 7789 from: 1 to: 6823 pFN21AB3223 6823 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4166 1937 Size: 2229 4594 Acc65I G'GTAC_C Cuts at: 3119 4144 3119 Size: 1025 5798 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2056 2433 4886 6270 6538 1632 Size: 424 377 2453 1384 268 1917 Fragments arranged by size: 2453 1917 1384 424 377 268 AfeI AGC'GCT Cuts at: 2526 2526 Size: 6823 AflII C'TTAA_G Cuts at: 820 1017 4013 820 Size: 197 2996 3630 Fragments arranged by size: 3630 2996 197 AflIII A'CryG_T Cuts at: 1203 2781 3841 5722 6699 1203 Size: 1578 1060 1881 977 1327 Fragments arranged by size: 1881 1578 1327 1060 977 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 3691 5552 3691 Size: 1861 4962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6823 AlwNI CAG_nnn'CTG Cuts at: 4566 6138 4566 Size: 1572 5251 ApaLI G'TGCA_C Cuts at: 4880 6036 4880 Size: 1156 5667 ApoI r'AATT_y Cuts at: 1493 2602 2757 4132 4358 4510 6619 1493 Size: 1109 155 1375 226 152 2109 1697 Fragments arranged by size: 2109 1697 1375 1109 226 155 152 AseI AT'TA_AT Cuts at: 160 1032 3855 5377 160 Size: 872 2823 1522 1606 Fragments arranged by size: 2823 1606 1522 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 4148 1870 Size: 73 2205 4545 Fragments arranged by size: 4545 2205 73 BamHI G'GATC_C Cuts at: 4153 4520 4153 Size: 367 6456 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2560 3119 4144 5599 618 Size: 325 217 627 773 559 1025 1455 1842 Fragments arranged by size: 1842 1455 1025 773 627 559 325 217 BanII G_rGCy'C Cuts at: 1391 2284 2432 4142 1391 Size: 893 148 1710 4072 Fragments arranged by size: 4072 1710 893 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2867 3495 928 Size: 971 968 628 4256 Fragments arranged by size: 4256 971 968 628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5014 5048 1903 Size: 34 3077 34 3678 Fragments arranged by size: 3678 3077 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4715 5925 4715 Size: 1210 5613 BclI T'GATC_A Cuts at: 1548 2254 1548 Size: 706 6117 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4049 5434 136 Size: 107 122 71 631 605 2377 1385 1525 Fragments arranged by size: 2377 1525 1385 631 605 122 107 71 BglII A'GATC_T Cuts at: 2413 6818 2413 Size: 4405 2418 BlpI GC'TnA_GC Cuts at: 2054 4240 2054 Size: 2186 4637 Bme1580I G_kGCm'C Cuts at: 1790 4884 6040 1790 Size: 3094 1156 2573 Fragments arranged by size: 3094 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2135 4554 5512 Size: 416 53 83 399 24 718 2419 958 Cuts at: 5512 442 Size: 1753 Fragments arranged by size: 2419 1753 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2673 1056 Size: 1617 5206 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2853 2365 Size: 488 6335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3162 5483 1239 Size: 1923 2321 2579 Fragments arranged by size: 2579 2321 1923 Bpu10I CC'TnA_GC Cuts at: 2646 2646 Size: 6823 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2848 4948 5813 6111 2848 Size: 2100 865 298 3560 Fragments arranged by size: 3560 2100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2497 5486 882 Size: 617 998 2989 2219 Fragments arranged by size: 2989 2219 998 617 BsaAI yAC'GTr Cuts at: 493 1204 4614 493 Size: 711 3410 2702 Fragments arranged by size: 3410 2702 711 BsaBI GATnn'nnATC Cuts at: 6817 6817 Size: 6823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4630 5012 5647 275 Size: 53 83 186 936 3097 382 635 1451 Fragments arranged by size: 3097 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5256 5654 5928 6075 6486 5256 Size: 398 274 147 411 5593 Fragments arranged by size: 5593 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6598 Fragments arranged by size: 6598 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2181 2515 2934 1186 Size: 995 334 419 5075 Fragments arranged by size: 5075 995 419 334 BseYI C'CCAG_C Cuts at: 2013 2431 6026 6522 2013 Size: 418 3595 496 2314 Fragments arranged by size: 3595 2314 496 418 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4273 5034 6062 1540 Size: 275 186 2272 761 1028 2301 Fragments arranged by size: 2301 2272 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4884 4969 6040 4884 Size: 85 1071 5667 Fragments arranged by size: 5667 1071 85 BsmI GAATG_Cn' Cuts at: 2967 2992 3863 4334 4427 2967 Size: 25 871 471 93 5363 Fragments arranged by size: 5363 871 471 93 25 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2284 2432 4142 4884 4969 6040 1391 Size: 399 494 148 1710 742 85 1071 2174 Fragments arranged by size: 2174 1710 1071 742 494 399 148 85 BspEI T'CCGG_A Cuts at: 6486 6486 Size: 6823 BspHI T'CATG_A Cuts at: 2310 3664 4712 6442 2310 Size: 1354 1048 1730 2691 Fragments arranged by size: 2691 1730 1354 1048 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4178 844 Size: 3334 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4710 1394 Size: 429 2887 3507 Fragments arranged by size: 3507 2887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3243 5318 5492 65 Size: 1183 1995 2075 174 1396 Fragments arranged by size: 2075 1995 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5467 5654 1700 Size: 110 3657 187 2869 Fragments arranged by size: 3657 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2361 96 Size: 2265 4558 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 2869 3218 4883 5895 2869 Size: 349 1665 1012 3797 Fragments arranged by size: 3797 1665 1012 349 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6823 BstBI TT'CG_AA Cuts at: 5627 5627 Size: 6823 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6823 BstXI CCAn_nnnn'nTGG Cuts at: 4111 4111 Size: 6823 BstYI r'GATC_y Cuts at: 1972 2413 4153 4520 4905 4922 6374 6460 6472 Size: 441 1740 367 385 17 1452 86 12 Cuts at: 6472 6818 1972 Size: 346 1977 Fragments arranged by size: 1977 1740 1452 441 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3945 513 Size: 552 2880 3391 Fragments arranged by size: 3391 2880 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2093 2882 487 Size: 683 56 867 789 4428 Fragments arranged by size: 4428 867 789 683 56 BtsI GCAGTG_nn' Cuts at: 4067 4339 5133 5153 4067 Size: 272 794 20 5737 Fragments arranged by size: 5737 794 272 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6788 DraI TTT'AAA Cuts at: 4127 4127 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5830 809 Size: 507 4514 1802 Fragments arranged by size: 4514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 3131 3877 Size: 54 1425 50 275 18 198 1103 746 Cuts at: 3877 4270 5159 6788 8 Size: 393 889 1629 43 Fragments arranged by size: 1629 1425 1103 889 746 393 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4270 1537 Size: 275 2458 4090 Fragments arranged by size: 4090 2458 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3896 4753 1353 Size: 2543 857 3423 Fragments arranged by size: 3423 2543 857 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3826 5410 5784 5930 387 Size: 1064 138 2237 1584 374 146 1280 Fragments arranged by size: 2237 1584 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2433 3162 4886 5483 6270 6538 Size: 393 424 377 729 1724 597 787 268 Cuts at: 6538 1239 Size: 1524 Fragments arranged by size: 1724 1524 787 729 597 424 393 377 268 EcoNI CCTnn'n_nnAGG Cuts at: 3114 3114 Size: 6823 EcoO109I rG'GnC_Cy Cuts at: 2694 2766 3335 2694 Size: 72 569 6182 Fragments arranged by size: 6182 569 72 EcoRI G'AATT_C Cuts at: 2757 4132 4510 2757 Size: 1375 378 5070 Fragments arranged by size: 5070 1375 378 EcoRV GAT'ATC Cuts at: 3721 3721 Size: 6823 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 2528 5970 1678 Size: 850 3442 2531 Fragments arranged by size: 3442 2531 850 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 3224 3256 3677 Size: 32 135 32 826 32 794 32 421 Cuts at: 3677 3689 3692 3709 3721 3724 5464 5496 5538 Size: 12 3 17 12 3 1740 32 42 Cuts at: 5538 5570 1373 Size: 32 2626 Fragments arranged by size: 2626 1740 826 794 421 135 42 32 32 32 32 32 32 17 12 12 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2198 4167 4413 669 Size: 988 281 260 1969 246 3079 Fragments arranged by size: 3079 1969 988 281 260 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 4413 4413 Size: 6823 KpnI G_GTAC'C Cuts at: 3123 4148 3123 Size: 1025 5798 MfeI C'AATT_G Cuts at: 3405 4422 3405 Size: 1017 5806 MluI A'CGCG_T Cuts at: 3841 6699 3841 Size: 2858 3965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3076 3454 5937 6121 1305 Size: 1771 378 2483 184 2007 Fragments arranged by size: 2483 2007 1771 378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3133 10 Size: 54 1425 343 1301 3700 Fragments arranged by size: 3700 1425 1301 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2250 2262 2817 4781 5140 5299 518 Size: 648 1084 12 555 1964 359 159 2042 Fragments arranged by size: 2042 1964 1084 648 555 359 159 12 MspA1I CmG'CkG Cuts at: 1606 4239 4916 6064 6309 1606 Size: 2633 677 1148 245 2120 Fragments arranged by size: 2633 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6271 NdeI CA'TA_TG Cuts at: 387 4119 387 Size: 3732 3091 NheI G'CTAG_C Cuts at: 1052 2669 1052 Size: 1617 5206 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 2785 4181 5726 1132 Size: 97 1556 1396 1545 2229 Fragments arranged by size: 2229 1556 1545 1396 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6823 PciI A'CATG_T Cuts at: 2781 5722 2781 Size: 2941 3882 PflMI CCAn_nnn'nTGG Cuts at: 1108 3129 3140 3331 1108 Size: 2021 11 191 4600 Fragments arranged by size: 4600 2021 191 11 PfoI T'CCnGG_A Cuts at: 2495 2495 Size: 6823 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4924 6405 6437 4892 Size: 32 1481 32 5278 Fragments arranged by size: 5278 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2694 2766 3335 2694 Size: 72 569 6182 Fragments arranged by size: 6182 569 72 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 4393 4393 Size: 6823 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2899 2931 3601 3633 3736 3768 2899 Size: 32 670 32 103 32 5954 Fragments arranged by size: 5954 670 103 32 32 32 PstI C_TGCA'G Cuts at: 830 2524 4175 830 Size: 1694 1651 3478 Fragments arranged by size: 3478 1694 1651 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 SalI G'TCGA_C Cuts at: 1936 4165 1936 Size: 2229 4594 SanDI GG'GwC_CC Cuts at: 2694 2694 Size: 6823 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 3754 5071 6783 3754 Size: 1317 1712 3794 Fragments arranged by size: 3794 1712 1317 SfcI C'TryA_G Cuts at: 826 1045 2034 2520 4171 5306 5987 6178 826 Size: 219 989 486 1651 1135 681 191 1471 Fragments arranged by size: 1651 1471 1135 989 681 486 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 4150 1872 Size: 2278 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2827 4013 4927 5828 6090 820 Size: 197 926 884 1186 914 901 262 1553 Fragments arranged by size: 1553 1186 926 914 901 884 262 197 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 4181 1132 Size: 3049 3774 SspI AAT'ATT Cuts at: 5 52 4747 5 Size: 47 4695 2081 Fragments arranged by size: 4695 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6823 StyI C'CwwG_G Cuts at: 513 1065 4262 513 Size: 552 3197 3074 Fragments arranged by size: 3197 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2057 4850 4867 5020 5205 5650 325 Size: 1255 477 2793 17 153 185 445 1498 Fragments arranged by size: 2793 1498 1255 477 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2361 3530 3752 Size: 274 80 33 51 1443 384 1169 222 Cuts at: 3752 5069 6781 96 Size: 1317 1712 138 Fragments arranged by size: 1712 1443 1317 1169 384 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2860 2892 6783 6815 2860 Size: 32 3891 32 2868 Fragments arranged by size: 3891 2868 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3494 1316 Size: 2178 4645 XbaI T'CTAG_A Cuts at: 2222 3734 3964 4159 2222 Size: 1512 230 195 4886 Fragments arranged by size: 4886 1512 230 195 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2747 2747 Size: 6823 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 4148 1870 Size: 2278 4545 XmnI GAAnn'nnTTC Cuts at: 3660 4952 3660 Size: 1292 5531 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI