Restriction map for
pFN21AB3223
(Linear) (Six-base) MAPSORT of: KIBB3223.seq Check: 9883 from: 1 to: 2118
KIBB3223.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
Acc65I G'GTAC_C
Cuts at: 0 1114 2118
Size: 1114 1004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 428 2118
Size: 51 377 1690
Fragments arranged by size:
1690 377 51
AfeI AGC'GCT
Cuts at: 0 521 2118
Size: 521 1597
AflII C'TTAA_G
Cuts at: 0 2008 2118
Size: 2008 110
AflIII A'CryG_T
Cuts at: 0 776 1836 2118
Size: 776 1060 282
Fragments arranged by size:
1060 776 282
AhdI GACnn_n'nnGTC
Cuts at: 0 1686 2118
Size: 1686 432
ApoI r'AATT_y
Cuts at: 0 597 752 2118
Size: 597 155 1366
Fragments arranged by size:
1366 597 155
AseI AT'TA_AT
Cuts at: 0 1850 2118
Size: 1850 268
BanI G'GyrC_C
Cuts at: 0 555 1114 2118
Size: 555 559 1004
Fragments arranged by size:
1004 559 555
BanII G_rGCy'C
Cuts at: 0 279 427 2118
Size: 279 148 1691
Fragments arranged by size:
1691 279 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 862 1490 2118
Size: 862 628 628
Fragments arranged by size:
862 628 628
BclI T'GATC_A
Cuts at: 0 249 2118
Size: 249 1869
BglI GCCn_nnn'nGGC
Cuts at: 0 2044 2118
Size: 2044 74
BglII A'GATC_T
Cuts at: 0 408 2118
Size: 408 1710
BlpI GC'TnA_GC
Cuts at: 0 49 2118
Size: 49 2069
BmrI ACTGGGnnnn_n'
Cuts at: 0 130 2118
Size: 130 1988
BmtI G_CTAG'C
Cuts at: 0 668 2118
Size: 668 1450
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 848 2118
Size: 360 488 1270
Fragments arranged by size:
1270 488 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 2118
Size: 1157 961
Bpu10I CC'TnA_GC
Cuts at: 0 641 2118
Size: 641 1477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 2118
Size: 843 1275
BsaI GGTCTCn'nnnn_
Cuts at: 0 492 2118
Size: 492 1626
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 176 510 929 2118
Size: 176 334 419 1189
Fragments arranged by size:
1189 419 334 176
BseYI C'CCAG_C
Cuts at: 0 8 426 2118
Size: 8 418 1692
Fragments arranged by size:
1692 418 8
BsmI GAATG_Cn'
Cuts at: 0 962 987 1858 2118
Size: 962 25 871 260
Fragments arranged by size:
962 871 260 25
Bsp1286I G_dGCh'C
Cuts at: 0 279 427 2118
Size: 279 148 1691
Fragments arranged by size:
1691 279 148
BspHI T'CATG_A
Cuts at: 0 305 1659 2118
Size: 305 1354 459
Fragments arranged by size:
1354 459 305
BsrDI GCAATG_nn'
Cuts at: 0 1238 2118
Size: 1238 880
BsrGI T'GTAC_A
Cuts at: 0 356 2118
Size: 356 1762
BssSI C'ACGA_G
Cuts at: 0 864 1213 2118
Size: 864 349 905
Fragments arranged by size:
905 864 349
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2106 2118
Size: 2106 12
BstYI r'GATC_y
Cuts at: 0 408 2118
Size: 408 1710
BtgI C'CryG_G
Cuts at: 0 1940 2118
Size: 1940 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 88 877 2118
Size: 88 789 1241
Fragments arranged by size:
1241 789 88
BtsI GCAGTG_nn'
Cuts at: 0 2062 2118
Size: 2062 56
EaeI y'GGCC_r
Cuts at: 0 23 1126 1872 2118
Size: 23 1103 746 246
Fragments arranged by size:
1103 746 246 23
EarI CTCTTCn'nnn_
Cuts at: 0 1891 2118
Size: 1891 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1821 2118
Size: 1821 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 428 1157 2118
Size: 51 377 729 961
Fragments arranged by size:
961 729 377 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1109 2118
Size: 1109 1009
EcoO109I rG'GnC_Cy
Cuts at: 0 689 761 1330 2118
Size: 689 72 569 788
Fragments arranged by size:
788 689 569 72
EcoRI G'AATT_C
Cuts at: 0 752 2118
Size: 752 1366
EcoRV GAT'ATC
Cuts at: 0 1716 2118
Size: 1716 402
HaeII r_GCGC'y
Cuts at: 0 523 2118
Size: 523 1595
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 1219 1251 1672 1684 1687 1704
Size: 393 32 794 32 421 12 3 17
Cuts at: 1704 1716 1719 2118
Size: 12 3 399
Fragments arranged by size:
794 421 399 393 32 32 17 12
12 3 3
HincII GTy'rAC
Cuts at: 0 193 2118
Size: 193 1925
KpnI G_GTAC'C
Cuts at: 0 1118 2118
Size: 1118 1000
MfeI C'AATT_G
Cuts at: 0 1400 2118
Size: 1400 718
MluI A'CGCG_T
Cuts at: 0 1836 2118
Size: 1836 282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1449 2118
Size: 1071 378 669
Fragments arranged by size:
1071 669 378
MscI TGG'CCA
Cuts at: 0 1128 2118
Size: 1128 990
MslI CAynn'nnrTG
Cuts at: 0 245 257 812 2118
Size: 245 12 555 1306
Fragments arranged by size:
1306 555 245 12
NdeI CA'TA_TG
Cuts at: 0 2114 2118
Size: 2114 4
NheI G'CTAG_C
Cuts at: 0 664 2118
Size: 664 1454
NspI r_CATG'y
Cuts at: 0 780 2118
Size: 780 1338
PciI A'CATG_T
Cuts at: 0 776 2118
Size: 776 1342
PflMI CCAn_nnn'nTGG
Cuts at: 0 1124 1135 1326 2118
Size: 1124 11 191 792
Fragments arranged by size:
1124 792 191 11
PfoI T'CCnGG_A
Cuts at: 0 490 2118
Size: 490 1628
PpuMI rG'GwC_Cy
Cuts at: 0 689 761 1330 2118
Size: 689 72 569 788
Fragments arranged by size:
788 689 569 72
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 894 926 1596 1628 1731 1763 2118
Size: 894 32 670 32 103 32 355
Fragments arranged by size:
894 670 355 103 32 32 32
PstI C_TGCA'G
Cuts at: 0 519 2118
Size: 519 1599
SanDI GG'GwC_CC
Cuts at: 0 689 2118
Size: 689 1429
ScaI AGT'ACT
Cuts at: 0 1749 2118
Size: 1749 369
SfcI C'TryA_G
Cuts at: 0 29 515 2118
Size: 29 486 1603
Fragments arranged by size:
1603 486 29
SmlI C'TyrA_G
Cuts at: 0 822 2008 2118
Size: 822 1186 110
Fragments arranged by size:
1186 822 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 52 2118
Size: 52 2066
TatI w'GTAC_w
Cuts at: 0 356 1525 1747 2118
Size: 356 1169 222 371
Fragments arranged by size:
1169 371 356 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 855 887 2118
Size: 855 32 1231
Fragments arranged by size:
1231 855 32
Tth111I GACn'n_nGTC
Cuts at: 0 1489 2118
Size: 1489 629
XbaI T'CTAG_A
Cuts at: 0 217 1729 1959 2118
Size: 217 1512 230 159
Fragments arranged by size:
1512 230 217 159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 742 2118
Size: 742 1376
XmnI GAAnn'nnTTC
Cuts at: 0 1655 2118
Size: 1655 463
Enzymes that do cut:
Acc65I AcuI AfeI AflII AflIII AhdI ApoI AseI
BanI BanII BbsI BclI BglI BglII BlpI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI
BsmI Bsp1286I BspHI BsrDI BsrGI BssSI BstXI BstYI
BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII KpnI MfeI
MluI MmeI MscI MslI NdeI NheI NspI PciI
PflMI PfoI PpuMI PsrI PstI SanDI ScaI SfcI
SmlI TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AgeI AleI AlfI AloI
AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI Bme1580I BmgBI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EcoICRI FalI FseI FspI FspAI HindIII
HpaI KasI MspA1I NaeI NarI NcoI NgoMIV NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI XhoI XmaI ZraI