Restriction map for
pFN21AB3218
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BclI BcnI BglI BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1407I BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CfoI Cfr10I CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AclI 2058 AACGTT AA^CGTT
AcsI 214 GAATTC [GA]^AATT[CT]
AfaI 1180 GTAC GT^AC
AflII 1066 CTTAAG C^TTAAG
AflIII 1623 ACATGT A^C[GA][CT]GT
Alw44I 581 GTGCAC G^TGCAC
ApaLI 581 GTGCAC G^TGCAC
ApoI 214 GAATTC [GA]^AATT[CT]
BanI 627 GGTGCC G^G[CT][GA]CC
BbsI 2638 GAAGAC GAAGAC(2/6)
BfrI 1066 CTTAAG C^TTAAG
BglII 1316 AGATCT A^GATCT
BpuAI 2638 GAAGAC GAAGAC(2/6)
BspDI 1915 ATCGAT AT^CGAT
BspHI 309 TCATGA T^CATGA
BspLU11I 1623 ACATGT A^CATGT
BspMI 1176 GCAGGT ACCTGC(4/8)
Bst1107I 574 GTATAC GTA^TAC
BstXI 563 CCATGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 574 GTATAC GTA^TAC
ClaI 1915 ATCGAT AT^CGAT
DraI 2398 TTTAAA TTT^AAA
DraII 1061 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 361 CCATGG C^C[GA][CT]GG
EcoO109I 1061 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 214 GAATTC G^AATTC
HgaI 231 GCGTC GACGC(5/10)
HindIII 826 AAGCTT A^AGCTT
NcoI 361 CCATGG C^CATGG
PpuMI 1061 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2058 AACGTT AA^CGTT
RcaI 309 TCATGA T^CATGA
RsaI 1180 GTAC GT^AC
SpeI 1300 ACTAGT A^CTAGT
XcmI 2369 CCATCAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 7 GCGG CCGC(-3/-1)
10 GCGG
Asp700I 916 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1426 GAACCTCTTC
BbvCI 1584 CCTCAGC CCTCAGC(-5/-2)
1258 GCTGAGG
BciVI 197 GTATCC GTATCC(6/5)
658 GTATCC
BlpI 352 GCTGAGC GC^T[ACGT]AGC
1359 GCTGAGC
Bpu1102I 352 GCTGAGC GC^T[ACGT]AGC
1359 GCTGAGC
BsgI 1533 GTGCAG GTGCAG(16/14)
2024 GTGCAG
BsmI 533 GAATGC GAATGC(1/-1)
1550 GCATTC
BstF5I 1203 GGATG GGATG(2/0)
1896 GGATG
Cac8I 2107 GCAAGC GC[ACGT]^[ACGT]GC
2610 GCTTGC
CelII 352 GCTGAGC GC^T[ACGT]AGC
1359 GCTGAGC
EcoNI 1936 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2422 CCTTTCCCAGG
EcoRV 487 GATATC GAT^ATC
2676 GATATC
FokI 1203 GGATG GGATG(9/13)
1896 GGATG
HincII 1027 GTTGAC GT[CT]^[GA]AC
2197 GTCAAC
HindII 1027 GTTGAC GT[CT]^[GA]AC
2197 GTCAAC
NspI 241 GCATGT [GA]CATG^[CT]
1623 ACATGT
PflMI 421 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1921 CCATTTGGTGG
PstI 347 CTGCAG CTGCA^G
665 CTGCAG
SacI 355 GAGCTC GAGCT^C
2232 GAGCTC
SapI 1354 GAAGAGC GCTCTTC(1/4)
2599 GAAGAGC
Tsp45I 1711 GTGAC ^GT[GC]AC
1729 GTGAC
Van91I 421 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1921 CCATTTGGTGG
XmnI 916 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1426 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccI AluI AlwI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstNI BstYI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII