Restriction map for
pFN21AB3218
(Linear) (Six-base) MAPSORT of: KIBB3218.seq Check: 3543 from: 1 to: 2688
KIBB3218.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 575 1368 1808 2688
Size: 575 793 440 880
Fragments arranged by size:
880 793 575 440
AclI AA'CG_TT
Cuts at: 0 2059 2688
Size: 2059 629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 1417 1959 2163 2520 2573 2688
Size: 398 1019 542 204 357 53 115
Fragments arranged by size:
1019 542 398 357 204 115 53
AflII C'TTAA_G
Cuts at: 0 1066 2688
Size: 1066 1622
AflIII A'CryG_T
Cuts at: 0 1623 2688
Size: 1623 1065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2627 2688
Size: 2627 61
ApaLI G'TGCA_C
Cuts at: 0 581 2688
Size: 581 2107
ApoI r'AATT_y
Cuts at: 0 214 2688
Size: 214 2474
BanI G'GyrC_C
Cuts at: 0 627 2688
Size: 627 2061
BanII G_rGCy'C
Cuts at: 0 359 1658 2236 2688
Size: 359 1299 578 452
Fragments arranged by size:
1299 578 452 359
BbsI GAAGACnn'nnnn_
Cuts at: 0 2645 2688
Size: 2645 43
BbvCI CC'TCA_GC
Cuts at: 0 1259 1585 2688
Size: 1259 326 1103
Fragments arranged by size:
1259 1103 326
BciVI GTATCCnnnnn_n'
Cuts at: 0 208 669 2688
Size: 208 461 2019
Fragments arranged by size:
2019 461 208
BglII A'GATC_T
Cuts at: 0 1316 2688
Size: 1316 1372
BlpI GC'TnA_GC
Cuts at: 0 353 1360 2688
Size: 353 1007 1328
Fragments arranged by size:
1328 1007 353
Bme1580I G_kGCm'C
Cuts at: 0 249 585 2688
Size: 249 336 2103
Fragments arranged by size:
2103 336 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 2688
Size: 415 2273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 607 1243 1658 2688
Size: 602 5 636 415 1030
Fragments arranged by size:
1030 636 602 415 5
Bpu10I CC'TnA_GC
Cuts at: 0 1259 1585 2688
Size: 1259 326 1103
Fragments arranged by size:
1259 1103 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 1797 2688
Size: 1219 578 891
Fragments arranged by size:
1219 891 578
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1172 1202 2688
Size: 1172 30 1486
Fragments arranged by size:
1486 1172 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 887 1240 1276 1408 2688
Size: 887 353 36 132 1280
Fragments arranged by size:
1280 887 353 132 36
BseYI C'CCAG_C
Cuts at: 0 14 1217 2304 2688
Size: 14 1203 1087 384
Fragments arranged by size:
1203 1087 384 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1554 2045 2688
Size: 1554 491 643
Fragments arranged by size:
1554 643 491
BsiHKAI G_wGCw'C
Cuts at: 0 359 585 2236 2688
Size: 359 226 1651 452
Fragments arranged by size:
1651 452 359 226
BsmI GAATG_Cn'
Cuts at: 0 539 1550 2688
Size: 539 1011 1138
Fragments arranged by size:
1138 1011 539
Bsp1286I G_dGCh'C
Cuts at: 0 249 359 585 1658 2236 2688
Size: 249 110 226 1073 578 452
Fragments arranged by size:
1073 578 452 249 226 110
BspHI T'CATG_A
Cuts at: 0 309 2688
Size: 309 2379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1167 2688
Size: 1167 1521
BstXI CCAn_nnnn'nTGG
Cuts at: 0 570 2688
Size: 570 2118
BstYI r'GATC_y
Cuts at: 0 1113 1316 1794 1950 2208 2365 2454 2688
Size: 1113 203 478 156 258 157 89 234
Fragments arranged by size:
1113 478 258 234 203 157 156 89
BstZ17I GTA'TAC
Cuts at: 0 576 2688
Size: 576 2112
BtgI C'CryG_G
Cuts at: 0 361 2688
Size: 361 2327
BtsI GCAGTG_nn'
Cuts at: 0 1605 1887 2308 2688
Size: 1605 282 421 380
Fragments arranged by size:
1605 421 380 282
ClaI AT'CG_AT
Cuts at: 0 1916 2688
Size: 1916 772
DraI TTT'AAA
Cuts at: 0 2400 2688
Size: 2400 288
EarI CTCTTCn'nnn_
Cuts at: 0 492 1284 1349 1436 2594 2688
Size: 492 792 65 87 1158 94
Fragments arranged by size:
1158 792 492 94 87 65
EcoICRI GAG'CTC
Cuts at: 0 357 2234 2688
Size: 357 1877 454
Fragments arranged by size:
1877 454 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 602 607 1243 1417 1658 1959 2163
Size: 398 204 5 636 174 241 301 204
Cuts at: 2163 2520 2573 2688
Size: 357 53 115
Fragments arranged by size:
636 398 357 301 241 204 204 174
115 53 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1940 2426 2688
Size: 1940 486 262
Fragments arranged by size:
1940 486 262
EcoO109I rG'GnC_Cy
Cuts at: 0 1062 2688
Size: 1062 1626
EcoRI G'AATT_C
Cuts at: 0 214 2688
Size: 214 2474
EcoRV GAT'ATC
Cuts at: 0 489 2678 2688
Size: 489 2189 10
Fragments arranged by size:
2189 489 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 725 757 1266 1298 2263 2295 2313 2345
Size: 725 32 509 32 965 32 18 32
Cuts at: 2345 2688
Size: 343
Fragments arranged by size:
965 725 509 343 32 32 32 32
18
HincII GTy'rAC
Cuts at: 0 1029 2199 2688
Size: 1029 1170 489
Fragments arranged by size:
1170 1029 489
HindIII A'AGCT_T
Cuts at: 0 826 2688
Size: 826 1862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 2356 2688
Size: 1403 953 332
Fragments arranged by size:
1403 953 332
MspA1I CmG'CkG
Cuts at: 0 352 1221 1738 2688
Size: 352 869 517 950
Fragments arranged by size:
950 869 517 352
NcoI C'CATG_G
Cuts at: 0 361 2688
Size: 361 2327
NspI r_CATG'y
Cuts at: 0 245 1627 2688
Size: 245 1382 1061
Fragments arranged by size:
1382 1061 245
PciI A'CATG_T
Cuts at: 0 1623 2688
Size: 1623 1065
PflMI CCAn_nnn'nTGG
Cuts at: 0 427 1927 2688
Size: 427 1500 761
Fragments arranged by size:
1500 761 427
PpuMI rG'GwC_Cy
Cuts at: 0 1062 2688
Size: 1062 1626
PstI C_TGCA'G
Cuts at: 0 351 669 2688
Size: 351 318 2019
Fragments arranged by size:
2019 351 318
PvuII CAG'CTG
Cuts at: 0 352 1221 1738 2688
Size: 352 869 517 950
Fragments arranged by size:
950 869 517 352
SacI G_AGCT'C
Cuts at: 0 359 2236 2688
Size: 359 1877 452
Fragments arranged by size:
1877 452 359
SapI GCTCTTCn'nnn_
Cuts at: 0 1349 2594 2688
Size: 1349 1245 94
Fragments arranged by size:
1349 1245 94
SfcI C'TryA_G
Cuts at: 0 66 347 665 1033 1159 1369 1568 1733
Size: 66 281 318 368 126 210 199 165
Cuts at: 1733 2688
Size: 955
Fragments arranged by size:
955 368 318 281 210 199 165 126
66
SmlI C'TyrA_G
Cuts at: 0 1066 1234 1812 2688
Size: 1066 168 578 876
Fragments arranged by size:
1066 876 578 168
SpeI A'CTAG_T
Cuts at: 0 1300 2688
Size: 1300 1388
SspI AAT'ATT
Cuts at: 0 404 752 2465 2688
Size: 404 348 1713 223
Fragments arranged by size:
1713 404 348 223
StyI C'CwwG_G
Cuts at: 0 361 492 623 848 2688
Size: 361 131 131 225 1840
Fragments arranged by size:
1840 361 225 131 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2271 2688
Size: 2271 417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2376 2688
Size: 2376 312
XmnI GAAnn'nnTTC
Cuts at: 0 920 1430 2688
Size: 920 510 1258
Fragments arranged by size:
1258 920 510
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AlfI ApaLI ApoI
BanI BanII BbsI BbvCI BciVI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BstXI BstYI BstZ17I
BtgI BtsI ClaI DraI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV Hin4I HincII HindIII MmeI MspA1I
NcoI NspI PciI PflMI PpuMI PstI PvuII SacI
SapI SfcI SmlI SpeI SspI StyI TaqII XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AleI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BclI BfrBI BglI BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII Bsu36I BtgZI CspCI DraIII DrdI
EaeI EagI EciI FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmaI ZraI