Restriction map for pFN21AB3118
 (Circular) (Six-base) MAPSORT of: pFN21AB3118.seq  Check: 169  from: 1  to: 7249



pFN21AB3118  7249 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:24  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    5059     278 
   Size:         53      83     186     936    3523    2468

  Fragments arranged by size: 

                3523    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4592    1937 
   Size:       2655    4594
 
Acc65I G'GTAC_C

Cuts at:   4570    4570 
   Size:       7249
 
AclI AA'CG_TT

Cuts at:   1563    5376    5749    1563 
   Size:       3813     373    3063

  Fragments arranged by size: 

                3813    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2109    2804    2958    3703    3777    4469    5312    6696 
   Size:        477     695     154     745      74     692     843    1384

Cuts at:   6696    6964    1632 
   Size:        268    1917

  Fragments arranged by size: 

                1917    1384     843     745     695     692     477     268
                 154      74
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    7052
 
AflIII A'CryG_T

Cuts at:   1203    4292    6148    7125    1203 
   Size:       3089    1856     977    1327

  Fragments arranged by size: 

                3089    1856    1327     977
 
AgeI A'CCGG_T

Cuts at:   6080    6080 
   Size:       7249
 
AhdI GACnn_n'nnGTC

Cuts at:   5978    5978 
   Size:       7249
 
AleI CACnn'nnGTG

Cuts at:   1166    4349    1166 
   Size:       3183    4066
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2965    1184 
   Size:       1781    5468
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4091    4123    4091 
   Size:         32    7217
 
AlwNI CAG_nnn'CTG

Cuts at:   2368    2561    4992    6564    2368 
   Size:        193    2431    1572    3053

  Fragments arranged by size: 

                3053    2431    1572     193
 
ApaLI G'TGCA_C

Cuts at:   2950    5306    6462    2950 
   Size:       2356    1156    3737

  Fragments arranged by size: 

                3737    2356    1156
 
ApoI r'AATT_y

Cuts at:   1493    2317    2351    2510    2716    2854    3683    4558    4784 
   Size:        824      34     159     206     138     829     875     226

Cuts at:   4784    4936    7045    1493 
   Size:        152    2109    1697

  Fragments arranged by size: 

                2109    1697     875     829     824     226     206     159
                 152     138      34
 
AseI AT'TA_AT

Cuts at:    160    1032    2187    5803     160 
   Size:        872    1155    3616    1606

  Fragments arranged by size: 

                3616    1606    1155     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7249
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4574    1870 
   Size:         73    2631    4545

  Fragments arranged by size: 

                4545    2631      73
 
AvrII C'CTAG_G

Cuts at:   3640    3640 
   Size:       7249
 
BamHI G'GATC_C

Cuts at:   3277    4579    4946    3277 
   Size:       1302     367    5580

  Fragments arranged by size: 

                5580    1302     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4570    6025     618 
   Size:        325     217     627    2783    1455    1842

  Fragments arranged by size: 

                2783    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3594    4568    1391 
   Size:       2203     974    4072

  Fragments arranged by size: 

                4072    2203     974
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2505    3133    3663     928 
   Size:        971     606     628     530    4514

  Fragments arranged by size: 

                4514     971     628     606     530
 
BbvCI CC'TCA_GC

Cuts at:   4338    4338 
   Size:       7249
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    5440    5474    1903 
   Size:         34    3503      34    3678

  Fragments arranged by size: 

                3678    3503      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   5141    6351    5141 
   Size:       1210    6039
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       7249
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5860     136 
   Size:        107     122      71     631     605    4188    1525

  Fragments arranged by size: 

                4188    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   7244    7244 
   Size:       7249
 
BlpI GC'TnA_GC

Cuts at:   2219    4666    2219 
   Size:       2447    4802
 
Bme1580I G_kGCm'C

Cuts at:   1790    2954    5310    6466    1790 
   Size:       1164    2356    1156    2573

  Fragments arranged by size: 

                2573    2356    1164    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7249
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2539    4980    5938 
   Size:        416      53      83     399      24    1122    2441     958

Cuts at:   5938     442 
   Size:       1753

  Fragments arranged by size: 

                2441    1753    1122     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    2160    1056 
   Size:       1104    6145
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2616    3054    3249    3597    5909    1239 
   Size:       1377     438     195     348    2312    2579

  Fragments arranged by size: 

                2579    2312    1377     438     348     195
 
Bpu10I CC'TnA_GC

Cuts at:   2962    4338    2962 
   Size:       1376    5873
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3814    5374    6239    6537    3814 
   Size:       1560     865     298    4526

  Fragments arranged by size: 

                4526    1560     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2118    2594    3148    5912     882 
   Size:        617     619     476     554    2764    2219

  Fragments arranged by size: 

                2764    2219     619     617     554     476
 
BsaAI yAC'GTr

Cuts at:    493    1204    4351    5040     493 
   Size:        711    3147     689    2702

  Fragments arranged by size: 

                3147    2702     711     689
 
BsaBI GATnn'nnATC

Cuts at:   7243    7243 
   Size:       7249
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    5056    5438    6073     275 
   Size:         53      83     186     936    3523     382     635    1451

  Fragments arranged by size: 

                3523    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2844    5682    6080    6354    6501    6912    2844 
   Size:       2838     398     274     147     411    3181

  Fragments arranged by size: 

                3181    2838     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3222    3252    1178 
   Size:         30     165      30    1819      30    5175

  Fragments arranged by size: 

                5175    1819     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    4275    1186 
   Size:       3089    4160
 
BseYI C'CCAG_C

Cuts at:   2083    4301    6452    6948    2083 
   Size:       2218    2151     496    2384

  Fragments arranged by size: 

                2384    2218    2151     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2081    3423    3489    1163 
   Size:        573     345    1342      66    4923

  Fragments arranged by size: 

                4923    1342     573     345      66
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4699    5460    6488    1540 
   Size:        275     186    2698     761    1028    2301

  Fragments arranged by size: 

                2698    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2954    5310    5395    6466    2954 
   Size:       2356      85    1071    3737

  Fragments arranged by size: 

                3737    2356    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3485    4760    4853    3485 
   Size:       1275      93    5881

  Fragments arranged by size: 

                5881    1275      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2954    3594    4568    5310    5395    6466    1391 
   Size:        399    1164     640     974     742      85    1071    2174

  Fragments arranged by size: 

                2174    1164    1071     974     742     640     399      85
            
 
BspEI T'CCGG_A

Cuts at:   2844    6912    2844 
   Size:       4068    3181
 
BspHI T'CATG_A

Cuts at:   5138    6868    5138 
   Size:       1730    5519
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    4604     844 
   Size:       3760    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    5136    1394 
   Size:        429    3313    3507

  Fragments arranged by size: 

                3507    3313     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2718    5744    5918      65 
   Size:       1183    1470    3026     174    1396

  Fragments arranged by size: 

                3026    1470    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2056    5893    6080    1700 
   Size:        110     246    3837     187    2869

  Fragments arranged by size: 

                3837    2869     246     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2552    3621    4145      96 
   Size:       2456    1069     524    3200

  Fragments arranged by size: 

                3200    2456    1069     524
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7249
 
BssSI C'ACGA_G

Cuts at:   2953    5309    6321    2953 
   Size:       2356    1012    3881

  Fragments arranged by size: 

                3881    2356    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       7249
 
BstBI TT'CG_AA

Cuts at:   6053    6053 
   Size:       7249
 
BstEII G'GTnAC_C

Cuts at:   1184    3581    1184 
   Size:       2397    4852
 
BstXI CCAn_nnnn'nTGG

Cuts at:   4253    4253 
   Size:       7249
 
BstYI r'GATC_y

Cuts at:   1972    3277    4579    4946    5331    5348    6800    6886    6898 
   Size:       1305    1302     367     385      17    1452      86      12

Cuts at:   6898    7244    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1305    1302     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3555    3555 
   Size:       7249
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    6697
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6510

  Fragments arranged by size: 

                6510     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4765    5559    5579    4765 
   Size:        794      20    6435

  Fragments arranged by size: 

                6435     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7249
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2772    2807     529 
   Size:         35    2208      35    4971

  Fragments arranged by size: 

                4971    2208      35      35
 
DraI TTT'AAA

Cuts at:   4401    4425    4553    4401 
   Size:         24     128    7097

  Fragments arranged by size: 

                7097     128      24
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6256     809 
   Size:        507    4940    1802

  Fragments arranged by size: 

                4940    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    4696    5585    7214 
   Size:         54    1425      50     275      18    2866     889    1629

Cuts at:   7214       8 
   Size:         43

  Fragments arranged by size: 

                2866    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4696    1537 
   Size:        275    2884    4090

  Fragments arranged by size: 

                4090    2884     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    5179    1353 
   Size:       3826    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2065    5836    6210    6356     387 
   Size:       1064     138     476    3771     374     146    1280

  Fragments arranged by size: 

                3771    1280    1064     476     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2109    2616    2804    2958    3054    3249    3597 
   Size:        393     477     507     188     154      96     195     348

Cuts at:   3597    3703    3777    4469    5312    5909    6696    6964    1239 
   Size:        106      74     692     843     597     787     268    1524

  Fragments arranged by size: 

                1524     843     787     692     597     507     477     393
                 348     268     195     188     154     106      96      74
            
 
EcoRI G'AATT_C

Cuts at:   2351    4558    4936    2351 
   Size:       2207     378    4664

  Fragments arranged by size: 

                4664    2207     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2266    3799    2266 
   Size:       1533    5716
 
FspI TGC'GCA

Cuts at:   5755    5755 
   Size:       7249
 
HaeII r_GCGC'y

Cuts at:   1678    2022    6396    1678 
   Size:        344    4374    2531

  Fragments arranged by size: 

                4374    2531     344
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2681    2713    2797    2829    3551 
   Size:         32     135      32    1109      32      84      32     722

Cuts at:   3551    3583    4517    4549    5890    5922    5964    5996    1373 
   Size:         32     934      32    1341      32      42      32    2626

  Fragments arranged by size: 

                2626    1341    1109     934     722     135      84      42
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3715    4593    4839     669 
   Size:        988     281    1777     878     246    3079

  Fragments arranged by size: 

                3079    1777     988     878     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       7249
 
HpaI GTT'AAC

Cuts at:   4839    4839 
   Size:       7249
 
KpnI G_GTAC'C

Cuts at:   4574    4574 
   Size:       7249
 
MfeI C'AATT_G

Cuts at:   3991    4848    3991 
   Size:        857    6392
 
MluI A'CGCG_T

Cuts at:   7125    7125 
   Size:       7249
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2124    2175    6363    6547    1305 
   Size:        819      51    4188     184    2007

  Fragments arranged by size: 

                4188    2007     819     184      51
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    5427

  Fragments arranged by size: 

                5427    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2472    4349    5207    5566    5725     518 
   Size:        648    1306    1877     858     359     159    2042

  Fragments arranged by size: 

                2042    1877    1306     858     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2087    2987    3916    4442    4665    5342    6490    6735 
   Size:        481     900     929     526     223     677    1148     245

Cuts at:   6735    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     929     900     677     526     481     245
                 223
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    6697
 
NdeI CA'TA_TG

Cuts at:    387    2666     387 
   Size:       2279    4970
 
NheI G'CTAG_C

Cuts at:   1052    2156    1052 
   Size:       1104    6145
 
NotI GC'GGCC_GC

Cuts at:   4696    4696 
   Size:       7249
 
NspI r_CATG'y

Cuts at:   1132    1229    3673    3940    3955    4296    4607    6152    1132 
   Size:         97    2444     267      15     341     311    1545    2229

  Fragments arranged by size: 

                2444    2229    1545     341     311     267      97      15
            
 
PasI CC'CwG_GG

Cuts at:   1348    3587    1348 
   Size:       2239    5010
 
PciI A'CATG_T

Cuts at:   4292    6148    4292 
   Size:       1856    5393
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       7249
 
PmeI GTTT'AAAC

Cuts at:   4553    4553 
   Size:       7249
 
PmlI CAC'GTG

Cuts at:   4351    4351 
   Size:       7249
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5318    5350    6831    6863    5318 
   Size:         32    1481      32    5704

  Fragments arranged by size: 

                5704    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2603    3089    3755    1613 
   Size:        990     486     666    5107

  Fragments arranged by size: 

                5107     990     666     486
 
PsiI TTA'TAA

Cuts at:   2613    3117    4819    2613 
   Size:        504    1702    5043

  Fragments arranged by size: 

                5043    1702     504
 
PstI C_TGCA'G

Cuts at:    830    4601     830 
   Size:       3771    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7249
 
PvuII CAG'CTG

Cuts at:   2087    2987    3916    4442    2087 
   Size:        900     929     526    4894

  Fragments arranged by size: 

                4894     929     900     526
 
SalI G'TCGA_C

Cuts at:   1936    4591    1936 
   Size:       2655    4594
 
SbfI CC_TGCA'GG

Cuts at:   4601    4601 
   Size:       7249
 
ScaI AGT'ACT

Cuts at:   3427    3436    4377    5497    7209    3427 
   Size:          9     941    1120    1712    3467

  Fragments arranged by size: 

                3467    1712    1120     941       9
 
SfcI C'TryA_G

Cuts at:    826    1045    2305    2394    4597    5732    6413    6604     826 
   Size:        219    1260      89    2203    1135     681     191    1471

  Fragments arranged by size: 

                2203    1471    1260    1135     681     219     191      89
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7249
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7249
 
SmaI CCC'GGG

Cuts at:   1872    4576    1872 
   Size:       2704    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3829    5353    6254    6516     820 
   Size:        197     926    1886    1524     901     262    1553

  Fragments arranged by size: 

                1886    1553    1524     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    5040     493 
   Size:       4547    2702
 
SpeI A'CTAG_T

Cuts at:    152    3308     152 
   Size:       3156    4093
 
SphI G_CATG'C

Cuts at:   1132    4607    1132 
   Size:       3475    3774
 
SspI AAT'ATT

Cuts at:      5      52    5173       5 
   Size:         47    5121    2081

  Fragments arranged by size: 

                5121    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2331    1517 
   Size:        814    6435
 
StyI C'CwwG_G

Cuts at:    513    1065    2722    3640    3816    4688     513 
   Size:        552    1657     918     176     872    3074

  Fragments arranged by size: 

                3074    1657     918     872     552     176
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2142    2517    3600    4361    5276    5293    5446 
   Size:       1255     562     375    1083     761     915      17     153

Cuts at:   5446    5631    6076     325 
   Size:        185     445    1498

  Fragments arranged by size: 

                1498    1255    1083     915     761     562     445     375
                 185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2552    3425    3434 
   Size:        274      80      33      51    1443     575     873       9

Cuts at:   3434    3621    4145    4365    4375    5495    7207      96 
   Size:        187     524     220      10    1120    1712     138

  Fragments arranged by size: 

                1712    1443    1120     873     575     524     274     220
                 187     138      80      51      33      10       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3222    3254    7209    7241    3222 
   Size:         32    3955      32    3230

  Fragments arranged by size: 

                3955    3230      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7249
 
XbaI T'CTAG_A

Cuts at:   3859    4585    3859 
   Size:        726    6523
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   4543    4543 
   Size:       7249
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7249
 
XmaI C'CCGG_G

Cuts at:   1870    4574    1870 
   Size:       2704    4545
 
XmnI GAAnn'nnTTC

Cuts at:   5378    5378 
   Size:       7249
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    5057     276 
   Size:         53      83     186     936    3523    2468

  Fragments arranged by size: 

                3523    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI     BaeI     BbeI    BfrBI     BplI
    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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