(Circular) (Six-base) MAPSORT of: pFN21AB3118.seq Check: 169 from: 1 to: 7249 pFN21AB3118 7249 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5059 278 Size: 53 83 186 936 3523 2468 Fragments arranged by size: 3523 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4592 1937 Size: 2655 4594 Acc65I G'GTAC_C Cuts at: 4570 4570 Size: 7249 AclI AA'CG_TT Cuts at: 1563 5376 5749 1563 Size: 3813 373 3063 Fragments arranged by size: 3813 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 2804 2958 3703 3777 4469 5312 6696 Size: 477 695 154 745 74 692 843 1384 Cuts at: 6696 6964 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 843 745 695 692 477 268 154 74 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7052 AflIII A'CryG_T Cuts at: 1203 4292 6148 7125 1203 Size: 3089 1856 977 1327 Fragments arranged by size: 3089 1856 1327 977 AgeI A'CCGG_T Cuts at: 6080 6080 Size: 7249 AhdI GACnn_n'nnGTC Cuts at: 5978 5978 Size: 7249 AleI CACnn'nnGTG Cuts at: 1166 4349 1166 Size: 3183 4066 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2965 1184 Size: 1781 5468 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4091 4123 4091 Size: 32 7217 AlwNI CAG_nnn'CTG Cuts at: 2368 2561 4992 6564 2368 Size: 193 2431 1572 3053 Fragments arranged by size: 3053 2431 1572 193 ApaLI G'TGCA_C Cuts at: 2950 5306 6462 2950 Size: 2356 1156 3737 Fragments arranged by size: 3737 2356 1156 ApoI r'AATT_y Cuts at: 1493 2317 2351 2510 2716 2854 3683 4558 4784 Size: 824 34 159 206 138 829 875 226 Cuts at: 4784 4936 7045 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 875 829 824 226 206 159 152 138 34 AseI AT'TA_AT Cuts at: 160 1032 2187 5803 160 Size: 872 1155 3616 1606 Fragments arranged by size: 3616 1606 1155 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7249 AvaI C'yCGr_G Cuts at: 1870 1943 4574 1870 Size: 73 2631 4545 Fragments arranged by size: 4545 2631 73 AvrII C'CTAG_G Cuts at: 3640 3640 Size: 7249 BamHI G'GATC_C Cuts at: 3277 4579 4946 3277 Size: 1302 367 5580 Fragments arranged by size: 5580 1302 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4570 6025 618 Size: 325 217 627 2783 1455 1842 Fragments arranged by size: 2783 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3594 4568 1391 Size: 2203 974 4072 Fragments arranged by size: 4072 2203 974 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2505 3133 3663 928 Size: 971 606 628 530 4514 Fragments arranged by size: 4514 971 628 606 530 BbvCI CC'TCA_GC Cuts at: 4338 4338 Size: 7249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5440 5474 1903 Size: 34 3503 34 3678 Fragments arranged by size: 3678 3503 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5141 6351 5141 Size: 1210 6039 BclI T'GATC_A Cuts at: 1548 1548 Size: 7249 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5860 136 Size: 107 122 71 631 605 4188 1525 Fragments arranged by size: 4188 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7244 7244 Size: 7249 BlpI GC'TnA_GC Cuts at: 2219 4666 2219 Size: 2447 4802 Bme1580I G_kGCm'C Cuts at: 1790 2954 5310 6466 1790 Size: 1164 2356 1156 2573 Fragments arranged by size: 2573 2356 1164 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7249 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2539 4980 5938 Size: 416 53 83 399 24 1122 2441 958 Cuts at: 5938 442 Size: 1753 Fragments arranged by size: 2441 1753 1122 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2160 1056 Size: 1104 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2616 3054 3249 3597 5909 1239 Size: 1377 438 195 348 2312 2579 Fragments arranged by size: 2579 2312 1377 438 348 195 Bpu10I CC'TnA_GC Cuts at: 2962 4338 2962 Size: 1376 5873 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 5374 6239 6537 3814 Size: 1560 865 298 4526 Fragments arranged by size: 4526 1560 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2118 2594 3148 5912 882 Size: 617 619 476 554 2764 2219 Fragments arranged by size: 2764 2219 619 617 554 476 BsaAI yAC'GTr Cuts at: 493 1204 4351 5040 493 Size: 711 3147 689 2702 Fragments arranged by size: 3147 2702 711 689 BsaBI GATnn'nnATC Cuts at: 7243 7243 Size: 7249 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5056 5438 6073 275 Size: 53 83 186 936 3523 382 635 1451 Fragments arranged by size: 3523 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2844 5682 6080 6354 6501 6912 2844 Size: 2838 398 274 147 411 3181 Fragments arranged by size: 3181 2838 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3222 3252 1178 Size: 30 165 30 1819 30 5175 Fragments arranged by size: 5175 1819 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4275 1186 Size: 3089 4160 BseYI C'CCAG_C Cuts at: 2083 4301 6452 6948 2083 Size: 2218 2151 496 2384 Fragments arranged by size: 2384 2218 2151 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 3423 3489 1163 Size: 573 345 1342 66 4923 Fragments arranged by size: 4923 1342 573 345 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4699 5460 6488 1540 Size: 275 186 2698 761 1028 2301 Fragments arranged by size: 2698 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2954 5310 5395 6466 2954 Size: 2356 85 1071 3737 Fragments arranged by size: 3737 2356 1071 85 BsmI GAATG_Cn' Cuts at: 3485 4760 4853 3485 Size: 1275 93 5881 Fragments arranged by size: 5881 1275 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2954 3594 4568 5310 5395 6466 1391 Size: 399 1164 640 974 742 85 1071 2174 Fragments arranged by size: 2174 1164 1071 974 742 640 399 85 BspEI T'CCGG_A Cuts at: 2844 6912 2844 Size: 4068 3181 BspHI T'CATG_A Cuts at: 5138 6868 5138 Size: 1730 5519 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4604 844 Size: 3760 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5136 1394 Size: 429 3313 3507 Fragments arranged by size: 3507 3313 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2718 5744 5918 65 Size: 1183 1470 3026 174 1396 Fragments arranged by size: 3026 1470 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 5893 6080 1700 Size: 110 246 3837 187 2869 Fragments arranged by size: 3837 2869 246 187 110 BsrGI T'GTAC_A Cuts at: 96 2552 3621 4145 96 Size: 2456 1069 524 3200 Fragments arranged by size: 3200 2456 1069 524 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7249 BssSI C'ACGA_G Cuts at: 2953 5309 6321 2953 Size: 2356 1012 3881 Fragments arranged by size: 3881 2356 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7249 BstBI TT'CG_AA Cuts at: 6053 6053 Size: 7249 BstEII G'GTnAC_C Cuts at: 1184 3581 1184 Size: 2397 4852 BstXI CCAn_nnnn'nTGG Cuts at: 4253 4253 Size: 7249 BstYI r'GATC_y Cuts at: 1972 3277 4579 4946 5331 5348 6800 6886 6898 Size: 1305 1302 367 385 17 1452 86 12 Cuts at: 6898 7244 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1305 1302 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3555 3555 Size: 7249 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6697 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6510 Fragments arranged by size: 6510 683 56 BtsI GCAGTG_nn' Cuts at: 4765 5559 5579 4765 Size: 794 20 6435 Fragments arranged by size: 6435 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7249 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2772 2807 529 Size: 35 2208 35 4971 Fragments arranged by size: 4971 2208 35 35 DraI TTT'AAA Cuts at: 4401 4425 4553 4401 Size: 24 128 7097 Fragments arranged by size: 7097 128 24 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6256 809 Size: 507 4940 1802 Fragments arranged by size: 4940 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4696 5585 7214 Size: 54 1425 50 275 18 2866 889 1629 Cuts at: 7214 8 Size: 43 Fragments arranged by size: 2866 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4696 1537 Size: 275 2884 4090 Fragments arranged by size: 4090 2884 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5179 1353 Size: 3826 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 5836 6210 6356 387 Size: 1064 138 476 3771 374 146 1280 Fragments arranged by size: 3771 1280 1064 476 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2616 2804 2958 3054 3249 3597 Size: 393 477 507 188 154 96 195 348 Cuts at: 3597 3703 3777 4469 5312 5909 6696 6964 1239 Size: 106 74 692 843 597 787 268 1524 Fragments arranged by size: 1524 843 787 692 597 507 477 393 348 268 195 188 154 106 96 74 EcoRI G'AATT_C Cuts at: 2351 4558 4936 2351 Size: 2207 378 4664 Fragments arranged by size: 4664 2207 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2266 3799 2266 Size: 1533 5716 FspI TGC'GCA Cuts at: 5755 5755 Size: 7249 HaeII r_GCGC'y Cuts at: 1678 2022 6396 1678 Size: 344 4374 2531 Fragments arranged by size: 4374 2531 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2681 2713 2797 2829 3551 Size: 32 135 32 1109 32 84 32 722 Cuts at: 3551 3583 4517 4549 5890 5922 5964 5996 1373 Size: 32 934 32 1341 32 42 32 2626 Fragments arranged by size: 2626 1341 1109 934 722 135 84 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3715 4593 4839 669 Size: 988 281 1777 878 246 3079 Fragments arranged by size: 3079 1777 988 878 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7249 HpaI GTT'AAC Cuts at: 4839 4839 Size: 7249 KpnI G_GTAC'C Cuts at: 4574 4574 Size: 7249 MfeI C'AATT_G Cuts at: 3991 4848 3991 Size: 857 6392 MluI A'CGCG_T Cuts at: 7125 7125 Size: 7249 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 2175 6363 6547 1305 Size: 819 51 4188 184 2007 Fragments arranged by size: 4188 2007 819 184 51 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5427 Fragments arranged by size: 5427 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2472 4349 5207 5566 5725 518 Size: 648 1306 1877 858 359 159 2042 Fragments arranged by size: 2042 1877 1306 858 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 2987 3916 4442 4665 5342 6490 6735 Size: 481 900 929 526 223 677 1148 245 Cuts at: 6735 1606 Size: 2120 Fragments arranged by size: 2120 1148 929 900 677 526 481 245 223 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6697 NdeI CA'TA_TG Cuts at: 387 2666 387 Size: 2279 4970 NheI G'CTAG_C Cuts at: 1052 2156 1052 Size: 1104 6145 NotI GC'GGCC_GC Cuts at: 4696 4696 Size: 7249 NspI r_CATG'y Cuts at: 1132 1229 3673 3940 3955 4296 4607 6152 1132 Size: 97 2444 267 15 341 311 1545 2229 Fragments arranged by size: 2444 2229 1545 341 311 267 97 15 PasI CC'CwG_GG Cuts at: 1348 3587 1348 Size: 2239 5010 PciI A'CATG_T Cuts at: 4292 6148 4292 Size: 1856 5393 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7249 PmeI GTTT'AAAC Cuts at: 4553 4553 Size: 7249 PmlI CAC'GTG Cuts at: 4351 4351 Size: 7249 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5318 5350 6831 6863 5318 Size: 32 1481 32 5704 Fragments arranged by size: 5704 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2603 3089 3755 1613 Size: 990 486 666 5107 Fragments arranged by size: 5107 990 666 486 PsiI TTA'TAA Cuts at: 2613 3117 4819 2613 Size: 504 1702 5043 Fragments arranged by size: 5043 1702 504 PstI C_TGCA'G Cuts at: 830 4601 830 Size: 3771 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7249 PvuII CAG'CTG Cuts at: 2087 2987 3916 4442 2087 Size: 900 929 526 4894 Fragments arranged by size: 4894 929 900 526 SalI G'TCGA_C Cuts at: 1936 4591 1936 Size: 2655 4594 SbfI CC_TGCA'GG Cuts at: 4601 4601 Size: 7249 ScaI AGT'ACT Cuts at: 3427 3436 4377 5497 7209 3427 Size: 9 941 1120 1712 3467 Fragments arranged by size: 3467 1712 1120 941 9 SfcI C'TryA_G Cuts at: 826 1045 2305 2394 4597 5732 6413 6604 826 Size: 219 1260 89 2203 1135 681 191 1471 Fragments arranged by size: 2203 1471 1260 1135 681 219 191 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7249 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7249 SmaI CCC'GGG Cuts at: 1872 4576 1872 Size: 2704 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3829 5353 6254 6516 820 Size: 197 926 1886 1524 901 262 1553 Fragments arranged by size: 1886 1553 1524 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5040 493 Size: 4547 2702 SpeI A'CTAG_T Cuts at: 152 3308 152 Size: 3156 4093 SphI G_CATG'C Cuts at: 1132 4607 1132 Size: 3475 3774 SspI AAT'ATT Cuts at: 5 52 5173 5 Size: 47 5121 2081 Fragments arranged by size: 5121 2081 47 StuI AGG'CCT Cuts at: 1517 2331 1517 Size: 814 6435 StyI C'CwwG_G Cuts at: 513 1065 2722 3640 3816 4688 513 Size: 552 1657 918 176 872 3074 Fragments arranged by size: 3074 1657 918 872 552 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2142 2517 3600 4361 5276 5293 5446 Size: 1255 562 375 1083 761 915 17 153 Cuts at: 5446 5631 6076 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1083 915 761 562 445 375 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2552 3425 3434 Size: 274 80 33 51 1443 575 873 9 Cuts at: 3434 3621 4145 4365 4375 5495 7207 96 Size: 187 524 220 10 1120 1712 138 Fragments arranged by size: 1712 1443 1120 873 575 524 274 220 187 138 80 51 33 10 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3222 3254 7209 7241 3222 Size: 32 3955 32 3230 Fragments arranged by size: 3955 3230 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7249 XbaI T'CTAG_A Cuts at: 3859 4585 3859 Size: 726 6523 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4543 4543 Size: 7249 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7249 XmaI C'CCGG_G Cuts at: 1870 4574 1870 Size: 2704 4545 XmnI GAAnn'nnTTC Cuts at: 5378 5378 Size: 7249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5057 276 Size: 53 83 186 936 3523 2468 Fragments arranged by size: 3523 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI