Restriction map for
pFN21AB3107
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI DdeI
DraI DraII DraIII Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FokI FseI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MflI MluI
MroI MslI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsaI SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 450 GAATTC [GA]^AATT[CT]
AflII 328 CTTAAG C^TTAAG
AlwI 210 GATCC GGATC(4/5)
Aor51HI 262 AGCGCT AGC^GCT
ApoI 450 GAATTC [GA]^AATT[CT]
AspHI 147 GTGCTC G[AT]GC[AT]^C
AvaI 369 CCCGGG C^[CT]CG[GA]G
AviII 67 TGCGCA TGC^GCA
BalI 441 TGGCCA TGG^CCA
BanI 137 GGTGCC G^G[CT][GA]CC
BfrI 328 CTTAAG C^TTAAG
BmyI 147 GTGCTC G[AGT]GC[ACT]^C
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsiHKAI 147 GTGCTC G[AT]GC[AT]^C
BsmFI 367 GTCCC GGGAC(10/14)
BsoBI 369 CCCGGG C^[CT]CG[GA]G
Bsp1286I 147 GTGCTC G[AGT]GC[ACT]^C
BspHI 299 TCATGA T^CATGA
BspMI 349 ACCTGC ACCTGC(4/8)
BsrDI 314 GCAATG GCAATG(2/0)
BstAPI 260 GCAGCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 92 CGGACCG CG^G[AT]CCG
DrdI 481 GACCAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 113 CCATGG C^C[GA][CT]GG
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco47III 262 AGCGCT AGC^GCT
Eco52I 41 CGGCCG C^GGCCG
EcoRI 450 GAATTC G^AATTC
EcoT14I 113 CCATGG C^C[AT][AT]GG
FauI 77 GCGGG CCCGC(4/6)
FspI 67 TGCGCA TGC^GCA
HaeII 262 AGCGCT [GA]GCGC^[CT]
MaeII 184 ACGT A^CGT
MluNI 441 TGGCCA TGG^CCA
MscI 441 TGGCCA TGG^CCA
MseI 329 TTAA T^TAA
MspA1I 166 CAGCTG C[AC]G^C[GT]G
NaeI 43 GCCGGC GCC^GGC
NcoI 113 CCATGG C^CATGG
NgoMIV 43 GCCGGC G^CCGGC
NspI 340 ACATGC [GA]CATG^[CT]
PvuII 166 CAGCTG CAG^CTG
RcaI 299 TCATGA T^CATGA
RsrII 92 CGGACCG CG^G[AT]CCG
SfcI 425 CTACAG C^T[GA][CT]AG
SmaI 369 CCCGGG CCC^GGG
StyI 113 CCATGG C^C[AT][AT]GG
TaqI 277 TCGA T^CGA
Tru9I 329 TTAA T^TAA
Tsp45I 37 GTGAC ^GT[GC]AC
Tsp509I 451 AATT ^AATT
TthHB8I 277 TCGA T^CGA
XmaCI 369 CCCGGG C^CCGGG
XmaI 369 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 93 GGACC G^G[AT]CC
366 GGTCC
BsrFI 43 GCCGGC [GA]^CCGG[CT]
134 GCCGGT
Cfr10I 43 GCCGGC [GA]^CCGG[CT]
134 GCCGGT
Cfr13I 93 GGACC G^G[ACGT]CC
366 GGTCC
DpnII 210 GATC ^GATC
376 GATC
EaeI 41 CGGCCG [CT]^GGCC[GA]
441 TGGCCA
HaeIII 42 GGCC GG^CC
442 GGCC
HphI 3 GGTGA GGTGA(8/7)
36 GGTGA
MaeIII 37 GTGAC ^GT[ACGT]AC
191 GTTAC
MboI 210 GATC ^GATC
376 GATC
NdeII 210 GATC ^GATC
376 GATC
Sau3AI 210 GATC ^GATC
376 GATC
Sau96I 93 GGACC G^G[ACGT]CC
366 GGTCC
SmlI 142 CTCAAG C^T[CT][GA]AG
328 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI BstNI Cac8I
CfoI EcoRII Fnu4HI HapII HhaI
HinP1I HinfI HpaII ItaI MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV ScrFI TfiI TseI