Restriction map for
pFN21AB3106
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII AccIII AcyI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
FbaI FseI FspI HgaI Hin1I
HpaI KasI KspI MluI MroI
NaeI NarI NdeI NgoMIV NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 2045 GGTACC G^GTACC
AccI 832 GTCTAC GT^[AC][GT]AC
AccII 945 CGCG CG^CG
AcsI 2107 AAATTT [GA]^AATT[CT]
Alw44I 1534 GTGCAC G^TGCAC
AocI 331 CCTAAGG CC^T[ACGT]AGG
ApaI 209 GGGCCC GGGCC^C
ApaLI 1534 GTGCAC G^TGCAC
ApoI 2107 AAATTT [GA]^AATT[CT]
Asp718I 2045 GGTACC G^GTACC
BcgI 1752 CGACTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 663 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 663 GCTGAGC GC^T[ACGT]AGC
BsaBI 2424 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2332 GAGACC GGTCTC(1/5)
BsgI 1332 CTGCAC GTGCAG(16/14)
BspMI 968 ACCTGC ACCTGC(4/8)
BsrDI 2190 CATTGC GCAATG(2/0)
BsrFI 1016 ACCGGC [GA]^CCGG[CT]
BssSI 1093 CACGAG CACGAG(-5/-1)
BstAPI 963 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 945 CGCG CG^CG
Bsu36I 331 CCTAAGG CC^T[ACGT]AGG
CelII 663 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1016 ACCGGC [GA]^CCGG[CT]
DrdI 1039 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 331 CCTAAGG CC^T[ACGT]AGG
EcoRV 2125 GATATC GAT^ATC
EcoT22I 1898 ATGCAT ATGCA^T
HindIII 463 AAGCTT A^AGCTT
KpnI 2045 GGTACC GGTAC^C
MamI 2424 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 533 CAATTG C^AATTG
MunI 533 CAATTG C^AATTG
MvnI 945 CGCG CG^CG
NheI 2296 GCTAGC G^CTAGC
NsiI 1898 ATGCAT ATGCA^T
NspI 2165 ACATGC [GA]CATG^[CT]
PshAI 1077 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1218 CCTGCAGG CCTGCA^GG
Sse8387I 1218 CCTGCAGG CCTGCA^GG
SspI 1471 AATATT AAT^ATT
TfiI 289 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AclI 868 AACGTT AA^CGTT
2152 AACGTT
Asp700I 96 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2220 GAAGGACTTC
AvaI 502 CTCGGG C^[CT]CG[GA]G
952 CCCGAG
BalI 1274 TGGCCA TGG^CCA
2504 TGGCCA
BanII 209 GGGCCC G[GA]GC[CT]^C
250 GGGCTC
BbvCI 1116 CCTCAGC CCTCAGC(-5/-2)
2341 GCTGAGG
BsiEI 1254 CGACCG CG[GA][CT]^CG
2433 CGACCG
BsoBI 502 CTCGGG C^[CT]CG[GA]G
952 CCCGAG
BspHI 1292 TCATGA T^CATGA
2129 TCATGA
BstBI 1210 TTCGAA TT^CGAA
1466 TTCGAA
DraIII 1479 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2037 CACCAAGTG
HaeII 119 AGCGCC [GA]GCGC^[CT]
1726 AGCGCC
MluNI 1274 TGGCCA TGG^CCA
2504 TGGCCA
MscI 1274 TGGCCA TGG^CCA
2504 TGGCCA
NcoI 1398 CCATGG C^CATGG
1660 CCATGG
NspV 1210 TTCGAA TT^CGAA
1466 TTCGAA
Psp1406I 868 AACGTT AA^CGTT
2152 AACGTT
RcaI 1292 TCATGA T^CATGA
2129 TCATGA
ScaI 1229 AGTACT AGT^ACT
1301 AGTACT
SfuI 1210 TTCGAA TT^CGAA
1466 TTCGAA
SmlI 253 CTCAAG C^T[CT][GA]AG
791 CTCAAG
XcmI 407 CCAAATCTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2039 CCAAGTGGTACCTGG
XmnI 96 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2220 GAAGGACTTC
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BamHI BanI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII