(Circular) (Six-base) MAPSORT of: pFN21AB3106.seq Check: 2689 from: 1 to: 7216 pFN21AB3106 7216 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5026 278 Size: 53 83 186 936 3490 2468 Fragments arranged by size: 3490 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2838 4559 1937 Size: 901 1721 4594 Fragments arranged by size: 4594 1721 901 Acc65I G'GTAC_C Cuts at: 4050 4537 4050 Size: 487 6729 AclI AA'CG_TT Cuts at: 1563 2874 4158 5343 5716 1563 Size: 1311 1284 1185 373 3063 Fragments arranged by size: 3063 1311 1284 1185 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2459 2667 3977 5279 6663 6931 1632 Size: 827 208 1310 1302 1384 268 1917 Fragments arranged by size: 1917 1384 1310 1302 827 268 208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7019 AflIII A'CryG_T Cuts at: 1203 6115 7092 1203 Size: 4912 977 1327 Fragments arranged by size: 4912 1327 977 AgeI A'CCGG_T Cuts at: 6047 6047 Size: 7216 AhdI GACnn_n'nnGTC Cuts at: 5945 5945 Size: 7216 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7216 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3918 1184 Size: 2734 4482 AlwNI CAG_nnn'CTG Cuts at: 3638 4000 4498 4959 6531 3638 Size: 362 498 461 1572 4323 Fragments arranged by size: 4323 1572 498 461 362 ApaI G_GGCC'C Cuts at: 2218 2218 Size: 7216 ApaLI G'TGCA_C Cuts at: 3539 5273 6429 3539 Size: 1734 1156 4326 Fragments arranged by size: 4326 1734 1156 ApoI r'AATT_y Cuts at: 1493 4112 4525 4751 4903 7012 1493 Size: 2619 413 226 152 2109 1697 Fragments arranged by size: 2619 2109 1697 413 226 152 AseI AT'TA_AT Cuts at: 160 1032 5770 160 Size: 872 4738 1606 Fragments arranged by size: 4738 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 AvaI C'yCGr_G Cuts at: 1870 1943 2507 2957 4541 1870 Size: 73 564 450 1584 4545 Fragments arranged by size: 4545 1584 564 450 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4042 4075 4042 Size: 33 7183 BamHI G'GATC_C Cuts at: 2585 3322 3657 4546 4913 2585 Size: 737 335 889 367 4888 Fragments arranged by size: 4888 889 737 367 335 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3347 3747 4050 4163 4477 Size: 325 217 627 1560 400 303 113 314 Cuts at: 4477 4537 5992 618 Size: 60 1455 1842 Fragments arranged by size: 1842 1560 1455 627 400 325 314 303 217 113 60 BanII G_rGCy'C Cuts at: 1391 2218 2259 4535 1391 Size: 827 41 2276 4072 Fragments arranged by size: 4072 2276 827 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3790 4357 4475 928 Size: 971 1891 567 118 3669 Fragments arranged by size: 3669 1891 971 567 118 BbvCI CC'TCA_GC Cuts at: 3122 4347 3122 Size: 1225 5991 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3746 3780 5407 5441 1903 Size: 34 1809 34 1627 34 3678 Fragments arranged by size: 3678 1809 1627 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5108 6318 5108 Size: 1210 6006 BclI T'GATC_A Cuts at: 1548 1548 Size: 7216 BfrBI ATG'CAT Cuts at: 3905 3905 Size: 7216 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5827 136 Size: 107 122 71 631 605 4155 1525 Fragments arranged by size: 4155 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7211 7211 Size: 7216 BlpI GC'TnA_GC Cuts at: 2669 4633 2669 Size: 1964 5252 Bme1580I G_kGCm'C Cuts at: 1790 2218 3208 3543 3846 4314 4482 5277 6433 Size: 428 990 335 303 468 168 795 1156 Cuts at: 6433 1790 Size: 2573 Fragments arranged by size: 2573 1156 990 795 468 428 335 303 168 BmgBI CAC'GTC Cuts at: 1318 4473 1318 Size: 3155 4061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 3481 3934 Size: 416 53 83 399 24 681 1383 453 Cuts at: 3934 4947 5905 442 Size: 1013 958 1753 Fragments arranged by size: 1753 1383 1013 958 681 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4305 1056 Size: 3249 3967 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2194 3376 2194 Size: 1182 6034 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2984 3092 3155 3713 3764 5876 1239 Size: 842 903 108 63 558 51 2112 2579 Fragments arranged by size: 2579 2112 903 842 558 108 63 51 Bpu10I CC'TnA_GC Cuts at: 3122 4347 3122 Size: 1225 5991 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 2781 5341 6206 6504 2243 Size: 538 2560 865 298 2955 Fragments arranged by size: 2955 2560 865 538 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 5879 882 Size: 617 2832 1548 2219 Fragments arranged by size: 2832 2219 1548 617 BsaAI yAC'GTr Cuts at: 493 1204 5007 493 Size: 711 3803 2702 Fragments arranged by size: 3803 2702 711 BsaBI GATnn'nnATC Cuts at: 4433 7210 4433 Size: 2777 4439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5023 5405 6040 275 Size: 53 83 186 936 3490 382 635 1451 Fragments arranged by size: 3490 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5649 6047 6321 6468 6879 5649 Size: 398 274 147 411 5986 Fragments arranged by size: 5986 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 1178 Size: 30 165 30 867 30 6094 Fragments arranged by size: 6094 867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2666 3116 3161 3257 3751 4049 4364 Size: 1105 375 450 45 96 494 298 315 Cuts at: 4364 1186 Size: 4038 Fragments arranged by size: 4038 1105 494 450 375 315 298 96 45 BseYI C'CCAG_C Cuts at: 2195 2384 2436 3752 3850 6419 6915 2195 Size: 189 52 1316 98 2569 496 2496 Fragments arranged by size: 2569 2496 1316 496 189 98 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3322 1163 Size: 573 1586 5057 Fragments arranged by size: 5057 1586 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4441 4666 5427 6455 1540 Size: 275 186 1261 1179 225 761 1028 2301 Fragments arranged by size: 2301 1261 1179 1028 761 275 225 186 BsiHKAI G_wGCw'C Cuts at: 2970 3543 4007 4186 4356 5277 5362 6433 2970 Size: 573 464 179 170 921 85 1071 3753 Fragments arranged by size: 3753 1071 921 573 464 179 170 85 BsmI GAATG_Cn' Cuts at: 2521 3821 3905 4727 4820 2521 Size: 1300 84 822 93 4917 Fragments arranged by size: 4917 1300 822 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2218 2259 2970 3208 3543 3846 4007 Size: 399 428 41 711 238 335 303 161 Cuts at: 4007 4186 4314 4356 4482 4535 5277 5362 6433 Size: 179 128 42 126 53 742 85 1071 Cuts at: 6433 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 711 428 399 335 303 238 179 161 128 126 85 53 42 41 BspEI T'CCGG_A Cuts at: 6879 6879 Size: 7216 BspHI T'CATG_A Cuts at: 3297 4134 5105 6835 3297 Size: 837 971 1730 3678 Fragments arranged by size: 3678 1730 971 837 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2982 4571 844 Size: 2138 1589 3489 Fragments arranged by size: 3489 2138 1589 BsrBI CCG'CTC Cuts at: 1394 1823 2065 3040 3106 3157 3628 4219 5103 Size: 429 242 975 66 51 471 591 884 Cuts at: 5103 1394 Size: 3507 Fragments arranged by size: 3507 975 884 591 471 429 242 66 51 BsrDI GCAATG_nn' Cuts at: 65 1248 4194 5711 5885 65 Size: 1183 2946 1517 174 1396 Fragments arranged by size: 2946 1517 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3021 5860 6047 1700 Size: 110 1211 2839 187 2869 Fragments arranged by size: 2869 2839 1211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7216 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7216 BssSI C'ACGA_G Cuts at: 3098 5276 6288 3098 Size: 2178 1012 4026 Fragments arranged by size: 4026 2178 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2974 794 Size: 2180 5036 BstBI TT'CG_AA Cuts at: 3216 3472 6020 3216 Size: 256 2548 4412 Fragments arranged by size: 4412 2548 256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7216 BstYI r'GATC_y Cuts at: 1972 2092 2585 3322 3558 3657 4546 4913 5298 Size: 120 493 737 236 99 889 367 385 Cuts at: 5298 5315 6767 6853 6865 7211 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 889 737 493 385 367 346 236 120 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2337 2337 Size: 7216 BtgI C'CryG_G Cuts at: 513 1065 2730 3403 3665 513 Size: 552 1665 673 262 4064 Fragments arranged by size: 4064 1665 673 552 262 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6477 Fragments arranged by size: 6477 683 56 BtsI GCAGTG_nn' Cuts at: 2772 4487 4732 5526 5546 2772 Size: 1715 245 794 20 4442 Fragments arranged by size: 4442 1715 794 245 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7216 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7181 DraI TTT'AAA Cuts at: 4520 4520 Size: 7216 DraIII CAC_nnn'GTG Cuts at: 3489 4047 3489 Size: 558 6658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3050 6223 809 Size: 507 1734 3173 1802 Fragments arranged by size: 3173 1802 1734 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3279 4296 4417 Size: 54 1425 50 275 18 1449 1017 121 Cuts at: 4417 4509 4663 5552 7181 8 Size: 92 154 889 1629 43 Fragments arranged by size: 1629 1449 1425 1017 889 275 154 121 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4663 1537 Size: 275 2851 4090 Fragments arranged by size: 4090 2851 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2301 2643 2730 3065 5146 1353 Size: 948 342 87 335 2081 3423 Fragments arranged by size: 3423 2081 948 342 335 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3175 3423 5803 6177 6323 387 Size: 1064 138 1586 248 2380 374 146 1280 Fragments arranged by size: 2380 1586 1280 1064 374 248 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2459 2667 2984 3092 3155 3713 Size: 393 449 378 208 317 108 63 558 Cuts at: 3713 3764 3977 5279 5876 6663 6931 1239 Size: 51 213 1302 597 787 268 1524 Fragments arranged by size: 1524 1302 787 597 558 449 393 378 317 268 213 208 108 63 51 EcoNI CCTnn'n_nnAGG Cuts at: 3125 3436 3680 3125 Size: 311 244 6661 Fragments arranged by size: 6661 311 244 EcoO109I rG'GnC_Cy Cuts at: 2503 2938 3088 3130 3415 4327 2503 Size: 435 150 42 285 912 5392 Fragments arranged by size: 5392 912 435 285 150 42 EcoRI G'AATT_C Cuts at: 4525 4903 4525 Size: 378 6838 EcoRV GAT'ATC Cuts at: 4132 4132 Size: 7216 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3325 3814 3994 3325 Size: 489 180 6547 Fragments arranged by size: 6547 489 180 FspI TGC'GCA Cuts at: 5722 5722 Size: 7216 HaeII r_GCGC'y Cuts at: 1678 2128 3735 6363 1678 Size: 450 1607 2628 2531 Fragments arranged by size: 2628 2531 1607 450 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2490 2522 3402 3434 5857 Size: 32 135 32 918 32 880 32 2423 Cuts at: 5857 5889 5931 5963 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2423 918 880 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2239 2629 3055 4560 4806 669 Size: 988 281 301 390 426 1505 246 3079 Fragments arranged by size: 3079 1505 988 426 390 301 281 246 HindIII A'AGCT_T Cuts at: 748 2468 748 Size: 1720 5496 HpaI GTT'AAC Cuts at: 4806 4806 Size: 7216 KpnI G_GTAC'C Cuts at: 4054 4541 4054 Size: 487 6729 MfeI C'AATT_G Cuts at: 2538 4815 2538 Size: 2277 4939 MluI A'CGCG_T Cuts at: 7092 7092 Size: 7216 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2268 3282 4040 4179 6330 6514 1305 Size: 963 1014 758 139 2151 184 2007 Fragments arranged by size: 2151 2007 1014 963 758 184 139 MscI TGG'CCA Cuts at: 10 64 1489 1832 3281 4511 10 Size: 54 1425 343 1449 1230 2715 Fragments arranged by size: 2715 1449 1425 1230 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2813 3900 4292 5174 5533 5692 518 Size: 648 1647 1087 392 882 359 159 2042 Fragments arranged by size: 2042 1647 1087 882 648 392 359 159 MspA1I CmG'CkG Cuts at: 1606 2273 2436 2911 2980 4632 5309 6457 6702 Size: 667 163 475 69 1652 677 1148 245 Cuts at: 6702 1606 Size: 2120 Fragments arranged by size: 2120 1652 1148 677 667 475 245 163 69 NcoI C'CATG_G Cuts at: 513 1065 3403 3665 513 Size: 552 2338 262 4064 Fragments arranged by size: 4064 2338 552 262 NdeI CA'TA_TG Cuts at: 387 387 Size: 7216 NheI G'CTAG_C Cuts at: 1052 4301 1052 Size: 3249 3967 NotI GC'GGCC_GC Cuts at: 4663 4663 Size: 7216 NsiI A_TGCA'T Cuts at: 3907 3907 Size: 7216 NspI r_CATG'y Cuts at: 1132 1229 4174 4574 6119 1132 Size: 97 2945 400 1545 2229 Fragments arranged by size: 2945 2229 1545 400 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7216 PciI A'CATG_T Cuts at: 6115 6115 Size: 7216 PflMI CCAn_nnn'nTGG Cuts at: 1108 2921 3070 4087 1108 Size: 1813 149 1017 4237 Fragments arranged by size: 4237 1813 1017 149 PfoI T'CCnGG_A Cuts at: 3090 3132 3090 Size: 42 7174 PmeI GTTT'AAAC Cuts at: 4520 4520 Size: 7216 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5285 5317 6798 6830 5285 Size: 32 1481 32 5671 Fragments arranged by size: 5671 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3088 3130 3415 4327 3088 Size: 42 285 912 5977 Fragments arranged by size: 5977 912 285 42 PshAI GACnn'nnGTC Cuts at: 1613 3086 1613 Size: 1473 5743 PsiI TTA'TAA Cuts at: 4786 4786 Size: 7216 PspOMI G'GGCC_C Cuts at: 2214 2214 Size: 7216 PstI C_TGCA'G Cuts at: 830 2979 3179 3228 3741 4494 4568 830 Size: 2149 200 49 513 753 74 3478 Fragments arranged by size: 3478 2149 753 513 200 74 49 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7216 PvuII CAG'CTG Cuts at: 2436 2911 2980 2436 Size: 475 69 6672 Fragments arranged by size: 6672 475 69 SalI G'TCGA_C Cuts at: 1936 4558 1936 Size: 2622 4594 SbfI CC_TGCA'GG Cuts at: 3228 4568 3228 Size: 1340 5876 ScaI AGT'ACT Cuts at: 3236 3308 5464 7176 3236 Size: 72 2156 1712 3276 Fragments arranged by size: 3276 2156 1712 72 SfcI C'TryA_G Cuts at: 826 1045 2842 2848 2897 2975 3029 3175 3224 Size: 219 1797 6 49 78 54 146 49 Cuts at: 3224 3737 4490 4564 5699 6380 6571 826 Size: 513 753 74 1135 681 191 1471 Fragments arranged by size: 1797 1471 1135 753 681 513 219 191 146 78 74 54 49 49 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7216 SmaI CCC'GGG Cuts at: 1872 4543 1872 Size: 2671 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 2796 5320 6221 6483 820 Size: 197 926 315 538 2524 901 262 1553 Fragments arranged by size: 2524 1553 926 901 538 315 262 197 SnaBI TAC'GTA Cuts at: 493 5007 493 Size: 4514 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7216 SphI G_CATG'C Cuts at: 1132 4574 1132 Size: 3442 3774 SspI AAT'ATT Cuts at: 5 52 3478 5140 5 Size: 47 3426 1662 2081 Fragments arranged by size: 3426 2081 1662 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7216 StyI C'CwwG_G Cuts at: 513 1065 2139 2351 2748 2934 3403 3497 3665 Size: 552 1074 212 397 186 469 94 168 Cuts at: 3665 4341 4512 4655 513 Size: 676 171 143 3074 Fragments arranged by size: 3074 1074 676 552 469 397 212 186 171 168 143 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3098 3500 3526 3678 3904 4223 4465 Size: 1255 1518 402 26 152 226 319 242 Cuts at: 4465 5243 5260 5413 5598 6043 325 Size: 778 17 153 185 445 1498 Fragments arranged by size: 1518 1498 1255 778 445 402 319 242 226 185 153 152 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3234 3246 3306 Size: 274 80 33 51 1443 1257 12 60 Cuts at: 3306 5462 7174 96 Size: 2156 1712 138 Fragments arranged by size: 2156 1712 1443 1257 274 138 80 60 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7176 7208 7176 Size: 32 7184 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7216 XbaI T'CTAG_A Cuts at: 4552 4552 Size: 7216 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2419 4051 2419 Size: 1632 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7216 XmaI C'CCGG_G Cuts at: 1870 4541 1870 Size: 2671 4545 XmnI GAAnn'nnTTC Cuts at: 2105 4229 5345 2105 Size: 2124 1116 3976 Fragments arranged by size: 3976 2124 1116 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5024 276 Size: 53 83 186 936 3490 2468 Fragments arranged by size: 3490 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI