Restriction map for
pFN21AB3106
(Linear) (Six-base) MAPSORT of: KIBB3106.seq Check: 7227 from: 1 to: 2511
KIBB3106.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 833 2511
Size: 833 1678
Acc65I G'GTAC_C
Cuts at: 0 2045 2511
Size: 2045 466
AclI AA'CG_TT
Cuts at: 0 869 2153 2511
Size: 869 1284 358
Fragments arranged by size:
1284 869 358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 662 1972 2511
Size: 454 208 1310 539
Fragments arranged by size:
1310 539 454 208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1913 2511
Size: 1913 598
AlwNI CAG_nnn'CTG
Cuts at: 0 1633 1995 2493 2511
Size: 1633 362 498 18
Fragments arranged by size:
1633 498 362 18
ApaI G_GGCC'C
Cuts at: 0 213 2511
Size: 213 2298
ApaLI G'TGCA_C
Cuts at: 0 1534 2511
Size: 1534 977
ApoI r'AATT_y
Cuts at: 0 2107 2511
Size: 2107 404
AvaI C'yCGr_G
Cuts at: 0 502 952 2511
Size: 502 450 1559
Fragments arranged by size:
1559 502 450
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2037 2070 2511
Size: 2037 33 441
Fragments arranged by size:
2037 441 33
BamHI G'GATC_C
Cuts at: 0 580 1317 1652 2511
Size: 580 737 335 859
Fragments arranged by size:
859 737 580 335
BanI G'GyrC_C
Cuts at: 0 1342 1742 2045 2158 2472 2511
Size: 1342 400 303 113 314 39
Fragments arranged by size:
1342 400 314 303 113 39
BanII G_rGCy'C
Cuts at: 0 213 254 2511
Size: 213 41 2257
Fragments arranged by size:
2257 213 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 1785 2352 2470 2511
Size: 1785 567 118 41
Fragments arranged by size:
1785 567 118 41
BbvCI CC'TCA_GC
Cuts at: 0 1117 2342 2511
Size: 1117 1225 169
Fragments arranged by size:
1225 1117 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1741 1775 2511
Size: 1741 34 736
Fragments arranged by size:
1741 736 34
BfrBI ATG'CAT
Cuts at: 0 1900 2511
Size: 1900 611
BlpI GC'TnA_GC
Cuts at: 0 664 2511
Size: 664 1847
Bme1580I G_kGCm'C
Cuts at: 0 213 1203 1538 1841 2309 2477 2511
Size: 213 990 335 303 468 168 34
Fragments arranged by size:
990 468 335 303 213 168 34
BmgBI CAC'GTC
Cuts at: 0 2468 2511
Size: 2468 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 1476 1929 2511
Size: 93 1383 453 582
Fragments arranged by size:
1383 582 453 93
BmtI G_CTAG'C
Cuts at: 0 2300 2511
Size: 2300 211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 189 1371 2511
Size: 189 1182 1140
Fragments arranged by size:
1182 1140 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 979 1087 1150 1708 1759 2511
Size: 76 903 108 63 558 51 752
Fragments arranged by size:
903 752 558 108 76 63 51
Bpu10I CC'TnA_GC
Cuts at: 0 1117 2342 2511
Size: 1117 1225 169
Fragments arranged by size:
1225 1117 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 776 2511
Size: 238 538 1735
Fragments arranged by size:
1735 538 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 2326 2511
Size: 2326 185
BsaBI GATnn'nnATC
Cuts at: 0 2428 2511
Size: 2428 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 2511
Size: 265 30 2216
Fragments arranged by size:
2216 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 661 1111 1156 1252 1746 2044 2359
Size: 286 375 450 45 96 494 298 315
Cuts at: 2359 2511
Size: 152
Fragments arranged by size:
494 450 375 315 298 286 152 96
45
BseYI C'CCAG_C
Cuts at: 0 190 379 431 1747 1845 2511
Size: 190 189 52 1316 98 666
Fragments arranged by size:
1316 666 190 189 98 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1317 2511
Size: 1317 1194
BsiEI CG_ry'CG
Cuts at: 0 1257 2436 2511
Size: 1257 1179 75
Fragments arranged by size:
1257 1179 75
BsiHKAI G_wGCw'C
Cuts at: 0 965 1538 2002 2181 2351 2511
Size: 965 573 464 179 170 160
Fragments arranged by size:
965 573 464 179 170 160
BsmI GAATG_Cn'
Cuts at: 0 516 1816 1900 2511
Size: 516 1300 84 611
Fragments arranged by size:
1300 611 516 84
Bsp1286I G_dGCh'C
Cuts at: 0 213 254 965 1203 1538 1841 2002 2181
Size: 213 41 711 238 335 303 161 179
Cuts at: 2181 2309 2351 2477 2511
Size: 128 42 126 34
Fragments arranged by size:
711 335 303 238 213 179 161 128
126 42 41 34
BspHI T'CATG_A
Cuts at: 0 1292 2129 2511
Size: 1292 837 382
Fragments arranged by size:
1292 837 382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 977 2511
Size: 977 1534
BsrBI CCG'CTC
Cuts at: 0 60 1035 1101 1152 1623 2214 2511
Size: 60 975 66 51 471 591 297
Fragments arranged by size:
975 591 471 297 66 60 51
BsrDI GCAATG_nn'
Cuts at: 0 2189 2511
Size: 2189 322
BsrFI r'CCGG_y
Cuts at: 0 1016 2511
Size: 1016 1495
BssSI C'ACGA_G
Cuts at: 0 1093 2511
Size: 1093 1418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 969 2511
Size: 969 1542
BstBI TT'CG_AA
Cuts at: 0 1211 1467 2511
Size: 1211 256 1044
Fragments arranged by size:
1211 1044 256
BstYI r'GATC_y
Cuts at: 0 87 580 1317 1553 1652 2511
Size: 87 493 737 236 99 859
Fragments arranged by size:
859 737 493 236 99 87
Bsu36I CC'TnA_GG
Cuts at: 0 332 2511
Size: 332 2179
BtgI C'CryG_G
Cuts at: 0 725 1398 1660 2511
Size: 725 673 262 851
Fragments arranged by size:
851 725 673 262
BtsI GCAGTG_nn'
Cuts at: 0 767 2482 2511
Size: 767 1715 29
Fragments arranged by size:
1715 767 29
DraIII CAC_nnn'GTG
Cuts at: 0 1484 2042 2511
Size: 1484 558 469
Fragments arranged by size:
1484 558 469
DrdI GACnn_nn'nnGTC
Cuts at: 0 1045 2511
Size: 1045 1466
EaeI y'GGCC_r
Cuts at: 0 1274 2291 2412 2504 2511
Size: 1274 1017 121 92 7
Fragments arranged by size:
1274 1017 121 92 7
EarI CTCTTCn'nnn_
Cuts at: 0 296 638 725 1060 2511
Size: 296 342 87 335 1451
Fragments arranged by size:
1451 342 335 296 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1170 1418 2511
Size: 1170 248 1093
Fragments arranged by size:
1170 1093 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 454 662 979 1087 1150 1708 1759
Size: 76 378 208 317 108 63 558 51
Cuts at: 1759 1972 2511
Size: 213 539
Fragments arranged by size:
558 539 378 317 213 208 108 76
63 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1120 1431 1675 2511
Size: 1120 311 244 836
Fragments arranged by size:
1120 836 311 244
EcoO109I rG'GnC_Cy
Cuts at: 0 498 933 1083 1125 1410 2322 2511
Size: 498 435 150 42 285 912 189
Fragments arranged by size:
912 498 435 285 189 150 42
EcoRV GAT'ATC
Cuts at: 0 2127 2511
Size: 2127 384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1320 1809 1989 2511
Size: 1320 489 180 522
Fragments arranged by size:
1320 522 489 180
HaeII r_GCGC'y
Cuts at: 0 123 1730 2511
Size: 123 1607 781
Fragments arranged by size:
1607 781 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 485 517 1397 1429 2511
Size: 485 32 880 32 1082
Fragments arranged by size:
1082 880 485 32 32
HincII GTy'rAC
Cuts at: 0 234 624 1050 2511
Size: 234 390 426 1461
Fragments arranged by size:
1461 426 390 234
HindIII A'AGCT_T
Cuts at: 0 463 2511
Size: 463 2048
KpnI G_GTAC'C
Cuts at: 0 2049 2511
Size: 2049 462
MfeI C'AATT_G
Cuts at: 0 533 2511
Size: 533 1978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1277 2035 2174 2511
Size: 263 1014 758 139 337
Fragments arranged by size:
1014 758 337 263 139
MscI TGG'CCA
Cuts at: 0 1276 2506 2511
Size: 1276 1230 5
Fragments arranged by size:
1276 1230 5
MslI CAynn'nnrTG
Cuts at: 0 808 1895 2287 2511
Size: 808 1087 392 224
Fragments arranged by size:
1087 808 392 224
MspA1I CmG'CkG
Cuts at: 0 268 431 906 975 2511
Size: 268 163 475 69 1536
Fragments arranged by size:
1536 475 268 163 69
NcoI C'CATG_G
Cuts at: 0 1398 1660 2511
Size: 1398 262 851
Fragments arranged by size:
1398 851 262
NheI G'CTAG_C
Cuts at: 0 2296 2511
Size: 2296 215
NsiI A_TGCA'T
Cuts at: 0 1902 2511
Size: 1902 609
NspI r_CATG'y
Cuts at: 0 2169 2511
Size: 2169 342
PflMI CCAn_nnn'nTGG
Cuts at: 0 916 1065 2082 2511
Size: 916 149 1017 429
Fragments arranged by size:
1017 916 429 149
PfoI T'CCnGG_A
Cuts at: 0 1085 1127 2511
Size: 1085 42 1384
Fragments arranged by size:
1384 1085 42
PpuMI rG'GwC_Cy
Cuts at: 0 1083 1125 1410 2322 2511
Size: 1083 42 285 912 189
Fragments arranged by size:
1083 912 285 189 42
PshAI GACnn'nnGTC
Cuts at: 0 1081 2511
Size: 1081 1430
PspOMI G'GGCC_C
Cuts at: 0 209 2511
Size: 209 2302
PstI C_TGCA'G
Cuts at: 0 974 1174 1223 1736 2489 2511
Size: 974 200 49 513 753 22
Fragments arranged by size:
974 753 513 200 49 22
PvuII CAG'CTG
Cuts at: 0 431 906 975 2511
Size: 431 475 69 1536
Fragments arranged by size:
1536 475 431 69
SbfI CC_TGCA'GG
Cuts at: 0 1223 2511
Size: 1223 1288
ScaI AGT'ACT
Cuts at: 0 1231 1303 2511
Size: 1231 72 1208
Fragments arranged by size:
1231 1208 72
SfcI C'TryA_G
Cuts at: 0 837 843 892 970 1024 1170 1219 1732
Size: 837 6 49 78 54 146 49 513
Cuts at: 1732 2485 2511
Size: 753 26
Fragments arranged by size:
837 753 513 146 78 54 49 49
26 6
SmlI C'TyrA_G
Cuts at: 0 253 791 2511
Size: 253 538 1720
Fragments arranged by size:
1720 538 253
SspI AAT'ATT
Cuts at: 0 1473 2511
Size: 1473 1038
StyI C'CwwG_G
Cuts at: 0 134 346 743 929 1398 1492 1660 2336
Size: 134 212 397 186 469 94 168 676
Cuts at: 2336 2511
Size: 175
Fragments arranged by size:
676 469 397 212 186 175 168 134
94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1093 1495 1521 1673 1899 2218 2460 2511
Size: 1093 402 26 152 226 319 242 51
Fragments arranged by size:
1093 402 319 242 226 152 51 26
TatI w'GTAC_w
Cuts at: 0 1229 1241 1301 2511
Size: 1229 12 60 1210
Fragments arranged by size:
1229 1210 60 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 2046 2511
Size: 414 1632 465
Fragments arranged by size:
1632 465 414
XmnI GAAnn'nnTTC
Cuts at: 0 100 2224 2511
Size: 100 2124 287
Fragments arranged by size:
2124 287 100
Enzymes that do cut:
AccI Acc65I AclI AcuI AlfI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BfrBI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KpnI
MfeI MmeI MscI MslI MspA1I NcoI NheI NsiI
NspI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SbfI ScaI SfcI SmlI SspI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AgeI AhdI AleI
AloI AscI AseI AsiSI AvrII BbeI BciVI BclI
BglI BglII BsaAI BsaHI BsaWI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI EagI EcoICRI EcoRI FseI FspI FspAI HpaI
KasI MluI NaeI NarI NdeI NgoMIV NotI NruI
PacI PasI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI