(Circular) (Six-base) MAPSORT of: pFN21AB3100.seq Check: 2417 from: 1 to: 7273 pFN21AB3100 7273 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4357 4357 Size: 7273 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5083 278 Size: 53 83 186 936 3547 2468 Fragments arranged by size: 3547 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 4616 1937 Size: 888 1791 4594 Fragments arranged by size: 4594 1791 888 Acc65I G'GTAC_C Cuts at: 3365 4594 3365 Size: 1229 6044 AclI AA'CG_TT Cuts at: 1563 5400 5773 1563 Size: 3837 373 3063 Fragments arranged by size: 3837 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2493 2622 5336 6720 6988 1632 Size: 410 451 129 2714 1384 268 1917 Fragments arranged by size: 2714 1917 1384 451 410 268 129 AfeI AGC'GCT Cuts at: 3827 3887 3827 Size: 60 7213 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7076 AflIII A'CryG_T Cuts at: 1203 6172 7149 1203 Size: 4969 977 1327 Fragments arranged by size: 4969 1327 977 AgeI A'CCGG_T Cuts at: 6104 6104 Size: 7273 AhdI GACnn_n'nnGTC Cuts at: 6002 6002 Size: 7273 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7273 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7273 AlwNI CAG_nnn'CTG Cuts at: 2834 5016 6588 2834 Size: 2182 1572 3519 Fragments arranged by size: 3519 2182 1572 ApaLI G'TGCA_C Cuts at: 5330 6486 5330 Size: 1156 6117 ApoI r'AATT_y Cuts at: 1493 4582 4808 4960 7069 1493 Size: 3089 226 152 2109 1697 Fragments arranged by size: 3089 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5827 160 Size: 872 4795 1606 Fragments arranged by size: 4795 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7273 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2174 2246 2281 2679 3187 4598 Size: 73 127 104 72 35 398 508 1411 Cuts at: 4598 1870 Size: 4545 Fragments arranged by size: 4545 1411 508 398 127 104 73 72 35 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2331 2364 2619 2652 2331 Size: 33 255 33 6952 Fragments arranged by size: 6952 255 33 33 BamHI G'GATC_C Cuts at: 4603 4970 4603 Size: 367 6906 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3236 3365 4594 6049 618 Size: 325 217 627 1449 129 1229 1455 1842 Fragments arranged by size: 1842 1455 1449 1229 627 325 217 129 BanII G_rGCy'C Cuts at: 1391 2148 2213 2271 2743 3171 4078 4592 1391 Size: 757 65 58 472 428 907 514 4072 Fragments arranged by size: 4072 907 757 514 472 428 65 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2435 928 Size: 971 536 5766 Fragments arranged by size: 5766 971 536 BbvCI CC'TCA_GC Cuts at: 2589 4431 2589 Size: 1842 5431 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2396 2430 5464 5498 1903 Size: 34 459 34 3034 34 3678 Fragments arranged by size: 3678 3034 459 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3239 5165 6375 3239 Size: 1926 1210 4137 Fragments arranged by size: 4137 1926 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7273 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2248 2497 5884 Size: 107 122 71 631 605 576 249 3387 Cuts at: 5884 136 Size: 1525 Fragments arranged by size: 3387 1525 631 605 576 249 122 107 71 BglII A'GATC_T Cuts at: 7268 7268 Size: 7273 BlpI GC'TnA_GC Cuts at: 2094 4690 2094 Size: 2596 4677 Bme1580I G_kGCm'C Cuts at: 1790 2181 3239 5334 6490 1790 Size: 391 1058 2095 1156 2573 Fragments arranged by size: 2573 2095 1156 1058 391 BmgBI CAC'GTC Cuts at: 1318 3025 1318 Size: 1707 5566 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2934 3454 5004 Size: 416 53 83 399 24 1517 520 1550 Cuts at: 5004 5962 442 Size: 958 1753 Fragments arranged by size: 1753 1550 1517 958 520 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7273 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2227 2821 2920 2227 Size: 594 99 6580 Fragments arranged by size: 6580 594 99 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 3930 5933 1239 Size: 1001 1690 2003 2579 Fragments arranged by size: 2579 2003 1690 1001 Bpu10I CC'TnA_GC Cuts at: 2589 4431 2589 Size: 1842 5431 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 2471 4201 5398 6263 6561 2063 Size: 408 1730 1197 865 298 2775 Fragments arranged by size: 2775 1730 1197 865 408 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2192 2377 3553 5936 882 Size: 617 693 185 1176 2383 2219 Fragments arranged by size: 2383 2219 1176 693 617 185 BsaAI yAC'GTr Cuts at: 493 1204 2845 5064 493 Size: 711 1641 2219 2702 Fragments arranged by size: 2702 2219 1641 711 BsaBI GATnn'nnATC Cuts at: 2789 7267 2789 Size: 4478 2795 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 5080 5462 6097 Size: 53 83 186 936 618 2929 382 635 Cuts at: 6097 275 Size: 1451 Fragments arranged by size: 2929 1451 936 635 618 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5706 6104 6378 6525 6936 5706 Size: 398 274 147 411 6043 Fragments arranged by size: 6043 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4031 4061 1178 Size: 30 165 30 2628 30 4390 Fragments arranged by size: 4390 2628 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2138 2204 2395 2577 3972 3987 1186 Size: 871 81 66 191 182 1395 15 4472 Fragments arranged by size: 4472 1395 871 191 182 81 66 15 BseYI C'CCAG_C Cuts at: 2883 3432 3806 4017 4080 4128 4148 4436 4464 Size: 549 374 211 63 48 20 288 28 Cuts at: 4464 6476 6972 2883 Size: 2012 496 3184 Fragments arranged by size: 3184 2012 549 496 374 288 211 63 48 28 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6700 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4723 5484 6512 1540 Size: 275 186 2722 761 1028 2301 Fragments arranged by size: 2722 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2452 2562 5334 5419 6490 2148 Size: 304 110 2772 85 1071 2931 Fragments arranged by size: 2931 2772 1071 304 110 85 BsmI GAATG_Cn' Cuts at: 4784 4877 4784 Size: 93 7180 BsmBI CGTCTCn'nnnn_ Cuts at: 3541 3541 Size: 7273 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2181 2213 2271 2452 2562 2743 Size: 399 358 33 32 58 181 110 181 Cuts at: 2743 3171 3239 4078 4592 5334 5419 6490 1391 Size: 428 68 839 514 742 85 1071 2174 Fragments arranged by size: 2174 1071 839 742 514 428 399 358 181 181 110 85 68 58 33 32 BspEI T'CCGG_A Cuts at: 6936 6936 Size: 7273 BspHI T'CATG_A Cuts at: 5162 6892 5162 Size: 1730 5543 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4357 4628 844 Size: 3513 271 3489 Fragments arranged by size: 3513 3489 271 BsrBI CCG'CTC Cuts at: 1394 1823 2098 2140 2583 2704 5160 1394 Size: 429 275 42 443 121 2456 3507 Fragments arranged by size: 3507 2456 443 429 275 121 42 BsrDI GCAATG_nn' Cuts at: 65 1248 3063 5768 5942 65 Size: 1183 1815 2705 174 1396 Fragments arranged by size: 2705 1815 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2946 3408 5917 6104 1700 Size: 110 1136 462 2509 187 2869 Fragments arranged by size: 2869 2509 1136 462 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7273 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7273 BssSI C'ACGA_G Cuts at: 5333 6345 5333 Size: 1012 6261 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7273 BstBI TT'CG_AA Cuts at: 6077 6077 Size: 7273 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7273 BstYI r'GATC_y Cuts at: 1972 4603 4970 5355 5372 6824 6910 6922 7268 Size: 2631 367 385 17 1452 86 12 346 Cuts at: 7268 1972 Size: 1977 Fragments arranged by size: 2631 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2056 2304 3862 3919 513 Size: 552 939 52 248 1558 57 3867 Fragments arranged by size: 3867 1558 939 552 248 57 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6534 Fragments arranged by size: 6534 683 56 BtsI GCAGTG_nn' Cuts at: 3144 4475 4789 5583 5603 3144 Size: 1331 314 794 20 4814 Fragments arranged by size: 4814 1331 794 314 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7273 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3823 3858 529 Size: 35 3259 35 3944 Fragments arranged by size: 3944 3259 35 35 DraI TTT'AAA Cuts at: 4577 4577 Size: 7273 DraIII CAC_nnn'GTG Cuts at: 3462 4556 3462 Size: 1094 6179 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6280 809 Size: 507 4964 1802 Fragments arranged by size: 4964 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3427 4502 4720 Size: 54 1425 50 275 18 1597 1075 218 Cuts at: 4720 5609 7238 8 Size: 889 1629 43 Fragments arranged by size: 1629 1597 1425 1075 889 275 218 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4720 1537 Size: 275 2908 4090 Fragments arranged by size: 4090 2908 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2598 2889 5203 1353 Size: 1245 291 2314 3423 Fragments arranged by size: 3423 2314 1245 291 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4098 5860 6234 6380 387 Size: 1064 138 2509 1762 374 146 1280 Fragments arranged by size: 2509 1762 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 2146 Size: 7273 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2240 2493 2622 3930 5336 5933 Size: 393 410 198 253 129 1308 1406 597 Cuts at: 5933 6720 6988 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1406 1308 787 597 410 393 268 253 198 129 EcoNI CCTnn'n_nnAGG Cuts at: 3728 3728 Size: 7273 EcoO109I rG'GnC_Cy Cuts at: 3103 3613 3902 3103 Size: 510 289 6474 Fragments arranged by size: 6474 510 289 EcoRI G'AATT_C Cuts at: 4582 4960 4582 Size: 378 6895 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3106 3106 Size: 7273 FspI TGC'GCA Cuts at: 3136 5779 3136 Size: 2643 4630 HaeII r_GCGC'y Cuts at: 1678 2056 3829 3889 4224 6420 1678 Size: 378 1773 60 335 2196 2531 Fragments arranged by size: 2531 2196 1773 378 335 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4414 4446 5914 5946 5988 Size: 32 135 32 2842 32 1468 32 42 Cuts at: 5988 6020 1373 Size: 32 2626 Fragments arranged by size: 2842 2626 1468 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4617 4863 669 Size: 988 281 2679 246 3079 Fragments arranged by size: 3079 2679 988 281 246 HindIII A'AGCT_T Cuts at: 748 2475 748 Size: 1727 5546 HpaI GTT'AAC Cuts at: 4863 4863 Size: 7273 KpnI G_GTAC'C Cuts at: 3369 4598 3369 Size: 1229 6044 MfeI C'AATT_G Cuts at: 2709 4872 2709 Size: 2163 5110 MluI A'CGCG_T Cuts at: 7149 7149 Size: 7273 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 3223 3360 4074 6387 6571 1305 Size: 789 1129 137 714 2313 184 2007 Fragments arranged by size: 2313 2007 1129 789 714 184 137 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5451 Fragments arranged by size: 5451 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2728 5231 5590 5749 518 Size: 648 843 719 2503 359 159 2042 Fragments arranged by size: 2503 2042 843 719 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 3415 3432 3754 3864 3951 3990 4017 Size: 452 1357 17 322 110 87 39 27 Cuts at: 4017 4689 5366 6514 6759 1606 Size: 672 677 1148 245 2120 Fragments arranged by size: 2120 1357 1148 677 672 452 322 245 110 87 39 27 17 NaeI GCC'GGC Cuts at: 3410 3410 Size: 7273 NcoI C'CATG_G Cuts at: 513 1065 2004 2304 513 Size: 552 939 300 5482 Fragments arranged by size: 5482 939 552 300 NdeI CA'TA_TG Cuts at: 387 387 Size: 7273 NgoMIV G'CCGG_C Cuts at: 3408 3408 Size: 7273 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7273 NotI GC'GGCC_GC Cuts at: 4720 4720 Size: 7273 NruI TCG'CGA Cuts at: 2040 2040 Size: 7273 NspI r_CATG'y Cuts at: 1132 1229 3329 4631 6176 1132 Size: 97 2100 1302 1545 2229 Fragments arranged by size: 2229 2100 1545 1302 97 PasI CC'CwG_GG Cuts at: 1348 4175 1348 Size: 2827 4446 PciI A'CATG_T Cuts at: 6172 6172 Size: 7273 PflMI CCAn_nnn'nTGG Cuts at: 1108 3713 1108 Size: 2605 4668 PfoI T'CCnGG_A Cuts at: 3723 4541 3723 Size: 818 6455 PmeI GTTT'AAAC Cuts at: 4577 4577 Size: 7273 PmlI CAC'GTG Cuts at: 2845 2845 Size: 7273 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5342 5374 6855 6887 3722 Size: 32 1588 32 1481 32 4108 Fragments arranged by size: 4108 1588 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3613 3902 3613 Size: 289 6984 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7273 PsiI TTA'TAA Cuts at: 4843 4843 Size: 7273 PspXI vC'TCGA_Gb Cuts at: 2070 2070 Size: 7273 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2318 2350 4511 4543 2318 Size: 32 2161 32 5048 Fragments arranged by size: 5048 2161 32 32 PstI C_TGCA'G Cuts at: 830 2052 2106 2256 2879 3753 4625 830 Size: 1222 54 150 623 874 872 3478 Fragments arranged by size: 3478 1222 874 872 623 150 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7273 SacI G_AGCT'C Cuts at: 2148 2148 Size: 7273 SacII CC_GC'GG Cuts at: 2059 3865 2059 Size: 1806 5467 SalI G'TCGA_C Cuts at: 1936 4615 1936 Size: 2679 4594 SapI GCTCTTCn'nnn_ Cuts at: 2889 2889 Size: 7273 SbfI CC_TGCA'GG Cuts at: 4625 4625 Size: 7273 ScaI AGT'ACT Cuts at: 4481 5521 7233 4481 Size: 1040 1712 4521 Fragments arranged by size: 4521 1712 1040 SfcI C'TryA_G Cuts at: 826 1045 2048 2102 2252 2826 2875 3749 4003 Size: 219 1003 54 150 574 49 874 254 Cuts at: 4003 4153 4252 4621 5756 6437 6628 826 Size: 150 99 369 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 874 681 574 369 254 219 191 150 150 99 54 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7273 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7273 SmaI CCC'GGG Cuts at: 1872 2176 2248 3189 4600 1872 Size: 304 72 941 1411 4545 Fragments arranged by size: 4545 1411 941 304 72 SmlI C'TyrA_G Cuts at: 820 1017 1943 2070 2078 2486 4216 5377 6278 Size: 197 926 127 8 408 1730 1161 901 Cuts at: 6278 6540 820 Size: 262 1553 Fragments arranged by size: 1730 1553 1161 926 901 408 262 197 127 8 SnaBI TAC'GTA Cuts at: 493 5064 493 Size: 4571 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7273 SphI G_CATG'C Cuts at: 1132 4631 1132 Size: 3499 3774 SrfI GCCC'GGGC Cuts at: 2248 2248 Size: 7273 SspI AAT'ATT Cuts at: 5 52 2314 5197 5 Size: 47 2262 2883 2081 Fragments arranged by size: 2883 2262 2081 47 StuI AGG'CCT Cuts at: 1517 2076 2411 2524 1517 Size: 559 335 113 6266 Fragments arranged by size: 6266 559 335 113 StyI C'CwwG_G Cuts at: 513 1065 2004 2304 3090 4712 513 Size: 552 939 300 786 1622 3074 Fragments arranged by size: 3074 1622 939 786 552 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2216 2467 2897 3473 3639 4162 5300 Size: 1255 636 251 430 576 166 523 1138 Cuts at: 5300 5317 5470 5655 6100 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1138 636 576 523 445 430 251 185 166 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3351 3783 4479 Size: 274 80 33 51 1443 1374 432 696 Cuts at: 4479 4565 5519 7231 96 Size: 86 954 1712 138 Fragments arranged by size: 1712 1443 1374 954 696 432 274 138 86 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3009 3041 7233 7265 3009 Size: 32 4192 32 3017 Fragments arranged by size: 4192 3017 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7273 XbaI T'CTAG_A Cuts at: 4609 4609 Size: 7273 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3376 3376 Size: 7273 XhoI C'TCGA_G Cuts at: 1943 2070 1943 Size: 127 7146 XmaI C'CCGG_G Cuts at: 1870 2174 2246 3187 4598 1870 Size: 304 72 941 1411 4545 Fragments arranged by size: 4545 1411 941 304 72 XmnI GAAnn'nnTTC Cuts at: 2330 5402 2330 Size: 3072 4201 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5081 276 Size: 53 83 186 936 3547 2468 Fragments arranged by size: 3547 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NsiI PacI PspOMI PvuII RsrII SanDI SexAI SfiI SfoI SwaI