Restriction map for
pFN21AB3098
(Circular) (Six-base) MAPSORT of: pFN21AB3098.seq Check: 8052 from: 1 to: 6643
pFN21AB3098 6643 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4453 278
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 3986 1937
Size: 951 1098 4594
Fragments arranged by size:
4594 1098 951
Acc65I G'GTAC_C
Cuts at: 3964 3964
Size: 6643
AclI AA'CG_TT
Cuts at: 1563 4770 5143 1563
Size: 3207 373 3063
Fragments arranged by size:
3207 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3357 3663 4706 6090 6358 1632
Size: 1725 306 1043 1384 268 1917
Fragments arranged by size:
1917 1725 1384 1043 306 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6446
AflIII A'CryG_T
Cuts at: 1203 5542 6519 1203
Size: 4339 977 1327
Fragments arranged by size:
4339 1327 977
AgeI A'CCGG_T
Cuts at: 5474 5474
Size: 6643
AhdI GACnn_n'nnGTC
Cuts at: 5372 5372
Size: 6643
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6643
AlwNI CAG_nnn'CTG
Cuts at: 2077 2100 4386 5958 2077
Size: 23 2286 1572 2762
Fragments arranged by size:
2762 2286 1572 23
ApaI G_GGCC'C
Cuts at: 2223 2223
Size: 6643
ApaLI G'TGCA_C
Cuts at: 4700 5856 4700
Size: 1156 5487
ApoI r'AATT_y
Cuts at: 1493 2675 2897 2930 3626 3775 3952 4178 4330
Size: 1182 222 33 696 149 177 226 152
Cuts at: 4330 6439 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1182 696 226 222 177 152
149 33
AscI GG'CGCG_CC
Cuts at: 2471 2471
Size: 6643
AseI AT'TA_AT
Cuts at: 160 1032 2709 5197 160
Size: 872 1677 2488 1606
Fragments arranged by size:
2488 1677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
AvaI C'yCGr_G
Cuts at: 1870 1943 2216 2222 2240 2302 2515 2598 3968
Size: 73 273 6 18 62 213 83 1370
Cuts at: 3968 1870
Size: 4545
Fragments arranged by size:
4545 1370 273 213 83 73 62 18
6
BamHI G'GATC_C
Cuts at: 3973 4340 3973
Size: 367 6276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 2519 3964 5419 618
Size: 325 217 627 507 225 1445 1455 1842
Fragments arranged by size:
1842 1455 1445 627 507 325 225 217
BanII G_rGCy'C
Cuts at: 1391 2178 2207 2217 2223 3014 3962 1391
Size: 787 29 10 6 791 948 4072
Fragments arranged by size:
4072 948 791 787 29 10 6
BbeI G_GCGC'C
Cuts at: 2298 2298
Size: 6643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2805 3679 928
Size: 971 906 874 3892
Fragments arranged by size:
3892 971 906 874
BbvCI CC'TCA_GC
Cuts at: 3828 3828
Size: 6643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4834 4868 1903
Size: 34 2897 34 3678
Fragments arranged by size:
3678 2897 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2644 3597 4535 5745 2644
Size: 953 938 1210 3542
Fragments arranged by size:
3542 1210 953 938
BclI T'GATC_A
Cuts at: 1548 3112 1548
Size: 1564 5079
BfrBI ATG'CAT
Cuts at: 2973 2973
Size: 6643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 2481 5254
Size: 107 122 71 631 605 382 427 2773
Cuts at: 5254 136
Size: 1525
Fragments arranged by size:
2773 1525 631 605 427 382 122 107
71
BglII A'GATC_T
Cuts at: 6638 6638
Size: 6643
BlpI GC'TnA_GC
Cuts at: 4060 4060
Size: 6643
Bme1580I G_kGCm'C
Cuts at: 1790 2223 2522 4704 5860 1790
Size: 433 299 2182 1156 2573
Fragments arranged by size:
2573 2182 1156 433 299
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4374 5332 442
Size: 416 53 83 399 24 2957 958 1753
Fragments arranged by size:
2957 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6643
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2356 2197
Size: 159 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2946 3400 5303 1239
Size: 1052 655 454 1903 2579
Fragments arranged by size:
2579 1903 1052 655 454
Bpu10I CC'TnA_GC
Cuts at: 2015 3828 2015
Size: 1813 4830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2682 3265 3782 4768 5633 5931 2682
Size: 583 517 986 865 298 3394
Fragments arranged by size:
3394 986 865 583 517 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5306 882
Size: 617 3807 2219
Fragments arranged by size:
3807 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4434 493
Size: 711 3230 2702
Fragments arranged by size:
3230 2702 711
BsaBI GATnn'nnATC
Cuts at: 6637 6637
Size: 6643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2295 4450 4832 5467
Size: 53 83 186 936 762 2155 382 635
Cuts at: 5467 275
Size: 1451
Fragments arranged by size:
2155 1451 936 762 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2722 3852 5076 5474 5748 5895 6306 2722
Size: 1130 1224 398 274 147 411 3059
Fragments arranged by size:
3059 1224 1130 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2267 2297 1178
Size: 30 165 30 864 30 5524
Fragments arranged by size:
5524 864 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2123 2135 2138 2141 2144 2288 2356
Size: 934 3 12 3 3 3 144 68
Cuts at: 2356 2363 2366 2685 3446 1186
Size: 7 3 319 761 4383
Fragments arranged by size:
4383 934 761 319 144 68 12 7
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 5846 6342 5846
Size: 496 6147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2044 3753 1163
Size: 573 308 1709 4053
Fragments arranged by size:
4053 1709 573 308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2507 4093 4854 5882 1540
Size: 275 186 506 1586 761 1028 2301
Fragments arranged by size:
2301 1586 1028 761 506 275 186
BsiHKAI G_wGCw'C
Cuts at: 3841 4704 4789 5860 3841
Size: 863 85 1071 4624
Fragments arranged by size:
4624 1071 863 85
BsmI GAATG_Cn'
Cuts at: 3070 3700 3763 4154 4247 3070
Size: 630 63 391 93 5466
Fragments arranged by size:
5466 630 391 93 63
BsmBI CGTCTCn'nnnn_
Cuts at: 3339 3339
Size: 6643
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2207 2217 2223 2522 3014 3841
Size: 399 388 29 10 6 299 492 827
Cuts at: 3841 3962 4704 4789 5860 1391
Size: 121 742 85 1071 2174
Fragments arranged by size:
2174 1071 827 742 492 399 388 299
121 85 29 10 6
BspEI T'CCGG_A
Cuts at: 2722 6306 2722
Size: 3584 3059
BspHI T'CATG_A
Cuts at: 2902 3186 4532 6262 2902
Size: 284 1346 1730 3283
Fragments arranged by size:
3283 1730 1346 284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2851 3998 844
Size: 2007 1147 3489
Fragments arranged by size:
3489 2007 1147
BsrBI CCG'CTC
Cuts at: 1394 1823 2235 2496 3921 4530 1394
Size: 429 412 261 1425 609 3507
Fragments arranged by size:
3507 1425 609 429 412 261
BsrDI GCAATG_nn'
Cuts at: 65 1248 3690 5138 5312 65
Size: 1183 2442 1448 174 1396
Fragments arranged by size:
2442 1448 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2245 2374 2386 2422 5287 5474 1700
Size: 110 435 129 12 36 2865 187 2869
Fragments arranged by size:
2869 2865 435 187 129 110 36 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6643
BssHII G'CGCG_C
Cuts at: 1925 2471 1925
Size: 546 6097
BssSI C'ACGA_G
Cuts at: 2341 4703 5715 2341
Size: 2362 1012 3269
Fragments arranged by size:
3269 2362 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3821 794
Size: 3027 3616
BstBI TT'CG_AA
Cuts at: 5447 5447
Size: 6643
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6643
BstYI r'GATC_y
Cuts at: 1972 3217 3411 3661 3973 4340 4725 4742 6194
Size: 1245 194 250 312 367 385 17 1452
Cuts at: 6194 6280 6292 6638 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1245 385 367 346 312 250
194 86 17 12
BstZ17I GTA'TAC
Cuts at: 2889 2889
Size: 6643
Bsu36I CC'TnA_GG
Cuts at: 2746 2746
Size: 6643
BtgI C'CryG_G
Cuts at: 513 1065 2208 2456 2501 513
Size: 552 1143 248 45 4655
Fragments arranged by size:
4655 1143 552 248 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5904
Fragments arranged by size:
5904 683 56
BtsI GCAGTG_nn'
Cuts at: 3063 4159 4953 4973 3063
Size: 1096 794 20 4733
Fragments arranged by size:
4733 1096 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6608
DraI TTT'AAA
Cuts at: 3199 3382 3947 3199
Size: 183 565 5895
Fragments arranged by size:
5895 565 183
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2757 5650 809
Size: 507 1441 2893 1802
Fragments arranged by size:
2893 1802 1441 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 2504 3235
Size: 54 1425 50 275 18 310 364 731
Cuts at: 3235 3858 4090 4979 6608 8
Size: 623 232 889 1629 43
Fragments arranged by size:
1629 1425 889 731 623 364 310 275
232 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2504 4090 1537
Size: 275 692 1586 4090
Fragments arranged by size:
4090 1586 692 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2561 2648 2951 3555 3848 4573 1353
Size: 759 449 87 303 604 293 725 3423
Fragments arranged by size:
3423 759 725 604 449 303 293 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2345 5230 5604 5750 387
Size: 1064 138 756 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 756 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2946 3357 3400 3663 4706 5303
Size: 393 659 655 411 43 263 1043 597
Cuts at: 5303 6090 6358 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1043 787 659 655 597 411 393
268 263 43
EcoO109I rG'GnC_Cy
Cuts at: 2278 2278
Size: 6643
EcoRI G'AATT_C
Cuts at: 3952 4330 3952
Size: 378 6265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 6643
FseI GG_CCGG'CC
Cuts at: 2249 2249
Size: 6643
FspI TGC'GCA
Cuts at: 5149 5149
Size: 6643
HaeII r_GCGC'y
Cuts at: 1678 2230 2298 5790 1678
Size: 552 68 3492 2531
Fragments arranged by size:
3492 2531 552 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 3347 3379 3403
Size: 32 135 32 1028 32 715 32 24
Cuts at: 3403 3435 5284 5316 5358 5390 1373
Size: 32 1849 32 42 32 2626
Fragments arranged by size:
2626 1849 1028 715 135 42 32 32
32 32 32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 3987 4233 669
Size: 988 281 2049 246 3079
Fragments arranged by size:
3079 2049 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6643
HpaI GTT'AAC
Cuts at: 4233 4233
Size: 6643
KasI G'GCGC_C
Cuts at: 2294 2294
Size: 6643
KpnI G_GTAC'C
Cuts at: 3968 3968
Size: 6643
MfeI C'AATT_G
Cuts at: 4242 4242
Size: 6643
MluI A'CGCG_T
Cuts at: 6519 6519
Size: 6643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3390 5757 5941 1305
Size: 2085 2367 184 2007
Fragments arranged by size:
2367 2085 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3860 10
Size: 54 1425 343 2028 2793
Fragments arranged by size:
2793 2028 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3075 4601 4960 5119 518
Size: 648 1909 1526 359 159 2042
Fragments arranged by size:
2042 1909 1526 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2139 2188 2458 2503 3827 4059 4736 5884
Size: 533 49 270 45 1324 232 677 1148
Cuts at: 5884 6129 1606
Size: 245 2120
Fragments arranged by size:
2120 1324 1148 677 533 270 245 232
49 45
NaeI GCC'GGC
Cuts at: 2247 2388 2247
Size: 141 6502
NarI GG'CG_CC
Cuts at: 2295 2295
Size: 6643
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6091
NdeI CA'TA_TG
Cuts at: 387 3117 387
Size: 2730 3913
NgoMIV G'CCGG_C
Cuts at: 2245 2386 2245
Size: 141 6502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6643
NotI GC'GGCC_GC
Cuts at: 4090 4090
Size: 6643
NsiI A_TGCA'T
Cuts at: 2975 2975
Size: 6643
NspI r_CATG'y
Cuts at: 1132 1229 3404 4001 5546 1132
Size: 97 2175 597 1545 2229
Fragments arranged by size:
2229 2175 1545 597 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6643
PciI A'CATG_T
Cuts at: 5542 5542
Size: 6643
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6643
PmeI GTTT'AAAC
Cuts at: 3947 3947
Size: 6643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4712 4744 6225 6257 4712
Size: 32 1481 32 5098
Fragments arranged by size:
5098 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 2278
Size: 6643
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6643
PsiI TTA'TAA
Cuts at: 4213 4213
Size: 6643
PspOMI G'GGCC_C
Cuts at: 2219 2219
Size: 6643
PstI C_TGCA'G
Cuts at: 830 2096 3826 3995 830
Size: 1266 1730 169 3478
Fragments arranged by size:
3478 1730 1266 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6643
PvuII CAG'CTG
Cuts at: 3827 3827
Size: 6643
SacII CC_GC'GG
Cuts at: 2459 2504 2459
Size: 45 6598
SalI G'TCGA_C
Cuts at: 1936 3985 1936
Size: 2049 4594
SbfI CC_TGCA'GG
Cuts at: 3995 3995
Size: 6643
ScaI AGT'ACT
Cuts at: 4891 6603 4891
Size: 1712 4931
SfcI C'TryA_G
Cuts at: 826 1045 2092 3178 3822 3991 5126 5807 5998
Size: 219 1047 1086 644 169 1135 681 191
Cuts at: 5998 826
Size: 1471
Fragments arranged by size:
1471 1135 1086 1047 681 644 219 191
169
SfoI GGC'GCC
Cuts at: 2296 2296
Size: 6643
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
SgrAI Cr'CCGG_yG
Cuts at: 1700 2386 2422 1700
Size: 686 36 5921
Fragments arranged by size:
5921 686 36
SmaI CCC'GGG
Cuts at: 1872 2218 2242 2304 2517 3970 1872
Size: 346 24 62 213 1453 4545
Fragments arranged by size:
4545 1453 346 213 62 24
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2697 3244 3797 4747 5648 5910
Size: 197 926 754 547 553 950 901 262
Cuts at: 5910 820
Size: 1553
Fragments arranged by size:
1553 950 926 901 754 553 547 262
197
SnaBI TAC'GTA
Cuts at: 493 4434 493
Size: 3941 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6643
SphI G_CATG'C
Cuts at: 1132 4001 1132
Size: 2869 3774
SrfI GCCC'GGGC
Cuts at: 2218 2242 2304 2218
Size: 24 62 6557
Fragments arranged by size:
6557 62 24
SspI AAT'ATT
Cuts at: 5 52 2707 3137 3298 4567 5
Size: 47 2655 430 161 1269 2081
Fragments arranged by size:
2655 2081 1269 430 161 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6643
StyI C'CwwG_G
Cuts at: 513 1065 4082 513
Size: 552 3017 3074
Fragments arranged by size:
3074 3017 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3890 4670 4687 4840 5025 5470 325
Size: 1255 2310 780 17 153 185 445 1498
Fragments arranged by size:
2310 1498 1255 780 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4889 6601 96
Size: 274 80 33 51 1443 2912 1712 138
Fragments arranged by size:
2912 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6603 6635 6603
Size: 32 6611
Tth111I GACn'n_nGTC
Cuts at: 1316 2754 1316
Size: 1438 5205
XbaI T'CTAG_A
Cuts at: 3722 3979 3722
Size: 257 6386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6643
XmaI C'CCGG_G
Cuts at: 1870 2216 2240 2302 2515 3968 1870
Size: 346 24 62 213 1453 4545
Fragments arranged by size:
4545 1453 346 213 62 24
XmnI GAAnn'nnTTC
Cuts at: 2608 3549 3676 4772 2608
Size: 941 127 1096 4479
Fragments arranged by size:
4479 1096 941 127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4451 276
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BsiWI BstXI DraIII
EcoICRI EcoNI EcoRV FspAI NruI PacI PfoI PmlI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SwaI XcmI