(Circular) (Six-base) MAPSORT of: pFN21AB3051.seq Check: 4825 from: 1 to: 8350 pFN21AB3051 8350 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3414 4312 3414 Size: 898 7452 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2328 2696 6160 278 Size: 53 83 186 936 792 368 3464 2468 Fragments arranged by size: 3464 2468 936 792 368 186 83 53 AccI GT'mk_AC Cuts at: 1937 3156 4631 5577 5693 1937 Size: 1219 1475 946 116 4594 Fragments arranged by size: 4594 1475 1219 946 116 Acc65I G'GTAC_C Cuts at: 3170 4080 5671 3170 Size: 910 1591 5849 Fragments arranged by size: 5849 1591 910 AclI AA'CG_TT Cuts at: 1563 6477 6850 1563 Size: 4914 373 3063 Fragments arranged by size: 4914 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2165 2549 2588 3017 3743 5287 5299 6413 Size: 533 384 39 429 726 1544 12 1114 Cuts at: 6413 7797 8065 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1544 1384 1114 726 533 429 384 268 39 12 AfeI AGC'GCT Cuts at: 3796 3796 Size: 8350 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8153 AflIII A'CryG_T Cuts at: 1203 7249 8226 1203 Size: 6046 977 1327 Fragments arranged by size: 6046 1327 977 AgeI A'CCGG_T Cuts at: 7181 7181 Size: 8350 AhdI GACnn_n'nnGTC Cuts at: 3907 7079 3907 Size: 3172 5178 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8350 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2677 2645 Size: 32 8318 AlwNI CAG_nnn'CTG Cuts at: 2143 3049 4213 6093 7665 2143 Size: 906 1164 1880 1572 2828 Fragments arranged by size: 2828 1880 1572 1164 906 ApaI G_GGCC'C Cuts at: 3085 3085 Size: 8350 ApaLI G'TGCA_C Cuts at: 3399 6407 7563 3399 Size: 3008 1156 4186 Fragments arranged by size: 4186 3008 1156 ApoI r'AATT_y Cuts at: 1493 4972 5659 5885 6037 8146 1493 Size: 3479 687 226 152 2109 1697 Fragments arranged by size: 3479 2109 1697 687 226 152 AscI GG'CGCG_CC Cuts at: 4917 4917 Size: 8350 AseI AT'TA_AT Cuts at: 160 1032 6904 160 Size: 872 5872 1606 Fragments arranged by size: 5872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8350 AvaI C'yCGr_G Cuts at: 1870 1943 2537 2594 4093 5675 1870 Size: 73 594 57 1499 1582 4545 Fragments arranged by size: 4545 1582 1499 594 73 57 BamHI G'GATC_C Cuts at: 5680 6047 5680 Size: 367 7983 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2318 3170 4005 4080 4200 Size: 325 217 627 531 852 835 75 120 Cuts at: 4200 5671 7126 618 Size: 1471 1455 1842 Fragments arranged by size: 1842 1471 1455 852 835 627 531 325 217 120 75 BanII G_rGCy'C Cuts at: 1391 2790 3085 4061 4408 4692 5088 5135 5402 Size: 1399 295 976 347 284 396 47 267 Cuts at: 5402 5669 1391 Size: 267 4072 Fragments arranged by size: 4072 1399 976 396 347 295 284 267 267 47 BbeI G_GCGC'C Cuts at: 4009 4009 Size: 8350 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3005 3928 928 Size: 971 1106 923 5350 Fragments arranged by size: 5350 1106 971 923 BbvCI CC'TCA_GC Cuts at: 4608 4608 Size: 8350 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6541 6575 1903 Size: 34 4604 34 3678 Fragments arranged by size: 4604 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2204 6242 7452 2204 Size: 4038 1210 3102 Fragments arranged by size: 4038 3102 1210 BclI T'GATC_A Cuts at: 1548 2508 5334 1548 Size: 960 2826 4564 Fragments arranged by size: 4564 2826 960 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3629 3833 5117 Size: 107 122 71 631 605 1957 204 1284 Cuts at: 5117 6961 136 Size: 1844 1525 Fragments arranged by size: 1957 1844 1525 1284 631 605 204 122 107 71 BglII A'GATC_T Cuts at: 5451 8345 5451 Size: 2894 5456 BlpI GC'TnA_GC Cuts at: 5087 5767 5087 Size: 680 7670 Bme1580I G_kGCm'C Cuts at: 1790 3085 3296 3403 6411 7567 1790 Size: 1295 211 107 3008 1156 2573 Fragments arranged by size: 3008 2573 1295 1156 211 107 BmgBI CAC'GTC Cuts at: 1318 2814 3334 3772 1318 Size: 1496 520 438 5896 Fragments arranged by size: 5896 1496 520 438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2719 3673 3815 Size: 416 53 83 399 24 1302 954 142 Cuts at: 3815 5191 6081 7039 442 Size: 1376 890 958 1753 Fragments arranged by size: 1753 1376 1302 958 954 890 416 399 142 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8350 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3913 3913 Size: 8350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2600 3308 3722 4205 4235 4860 5069 5396 Size: 1361 708 414 483 30 625 209 327 Cuts at: 5396 7010 1239 Size: 1614 2579 Fragments arranged by size: 2579 1614 1361 708 625 483 414 327 209 30 Bpu10I CC'TnA_GC Cuts at: 3642 4432 4608 3642 Size: 790 176 7384 Fragments arranged by size: 7384 790 176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1997 2383 5057 5225 5410 6475 7340 7638 1997 Size: 386 2674 168 185 1065 865 298 2709 Fragments arranged by size: 2709 2674 1065 865 386 298 185 168 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2433 4472 7013 882 Size: 617 934 2039 2541 2219 Fragments arranged by size: 2541 2219 2039 934 617 BsaAI yAC'GTr Cuts at: 493 1204 3154 6141 493 Size: 711 1950 2987 2702 Fragments arranged by size: 2987 2702 1950 711 BsaBI GATnn'nnATC Cuts at: 2936 3128 8344 2936 Size: 192 5216 2942 Fragments arranged by size: 5216 2942 192 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2325 2693 3719 3876 Size: 53 83 186 936 792 368 1026 157 Cuts at: 3876 3958 4006 4734 5151 5181 5358 6157 6539 Size: 82 48 728 417 30 177 799 382 Cuts at: 6539 7174 275 Size: 635 1451 Fragments arranged by size: 1451 1026 936 799 792 728 635 417 382 368 186 177 157 83 82 53 48 30 BsaWI w'CCGG_w Cuts at: 6783 7181 7455 7602 8013 6783 Size: 398 274 147 411 7120 Fragments arranged by size: 7120 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3704 3734 5501 5531 1178 Size: 30 165 30 2301 30 1767 30 3997 Fragments arranged by size: 3997 2301 1767 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2819 3800 3959 3962 4202 4205 4243 4355 Size: 1633 981 159 3 240 3 38 112 Cuts at: 4355 4772 4850 4901 4940 4943 4952 5125 1186 Size: 417 78 51 39 3 9 173 4411 Fragments arranged by size: 4411 1633 981 417 240 173 159 112 78 51 39 38 9 3 3 3 BseYI C'CCAG_C Cuts at: 2223 2372 2767 3423 3654 3806 4317 4364 7553 Size: 149 395 656 231 152 511 47 3189 Cuts at: 7553 8049 2223 Size: 496 2524 Fragments arranged by size: 3189 2524 656 511 496 395 231 152 149 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3038 3593 3707 3728 4139 4928 5294 Size: 573 1302 555 114 21 411 789 366 Cuts at: 5294 5608 1163 Size: 314 3905 Fragments arranged by size: 3905 1302 789 573 555 411 366 314 114 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 3974 5126 5800 6561 7589 Size: 275 186 37 1936 1152 674 761 1028 Cuts at: 7589 1540 Size: 2301 Fragments arranged by size: 2301 1936 1152 1028 761 674 275 186 37 BsiHKAI G_wGCw'C Cuts at: 2746 2865 3403 4061 4107 4692 5088 5402 6411 Size: 119 538 658 46 585 396 314 1009 Cuts at: 6411 6496 7567 2746 Size: 85 1071 3529 Fragments arranged by size: 3529 1071 1009 658 585 538 396 314 119 85 46 BsmI GAATG_Cn' Cuts at: 4360 5418 5861 5954 4360 Size: 1058 443 93 6756 Fragments arranged by size: 6756 1058 443 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5188 5188 Size: 8350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2746 2790 2865 3085 3296 3403 4061 Size: 399 956 44 75 220 211 107 658 Cuts at: 4061 4107 4408 4692 5088 5135 5402 5669 6411 Size: 46 301 284 396 47 267 267 742 Cuts at: 6411 6496 7567 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 956 742 658 399 396 301 284 267 267 220 211 107 85 75 47 46 44 BspEI T'CCGG_A Cuts at: 8013 8013 Size: 8350 BspHI T'CATG_A Cuts at: 2457 6239 7969 2457 Size: 3782 1730 2838 Fragments arranged by size: 3782 2838 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3099 3414 4312 5705 844 Size: 2255 315 898 1393 3489 Fragments arranged by size: 3489 2255 1393 898 315 BsrBI CCG'CTC Cuts at: 1394 1823 2674 3715 4195 4840 5517 6237 1394 Size: 429 851 1041 480 645 677 720 3507 Fragments arranged by size: 3507 1041 851 720 677 645 480 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2988 5259 6845 7019 65 Size: 1183 1740 2271 1586 174 1396 Fragments arranged by size: 2271 1740 1586 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3669 3969 4203 4903 6994 7181 1700 Size: 110 1859 300 234 700 2091 187 2869 Fragments arranged by size: 2869 2091 1859 700 300 234 187 110 BsrGI T'GTAC_A Cuts at: 96 2514 5508 96 Size: 2418 2994 2938 Fragments arranged by size: 2994 2938 2418 BssHII G'CGCG_C Cuts at: 1925 4917 1925 Size: 2992 5358 BssSI C'ACGA_G Cuts at: 3941 5288 6410 7422 3941 Size: 1347 1122 1012 4869 Fragments arranged by size: 4869 1347 1122 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8350 BstBI TT'CG_AA Cuts at: 7154 7154 Size: 8350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8350 BstXI CCAn_nnnn'nTGG Cuts at: 2443 5047 2443 Size: 2604 5746 BstYI r'GATC_y Cuts at: 1972 2349 2991 3435 3465 5451 5680 6047 6432 Size: 377 642 444 30 1986 229 367 385 Cuts at: 6432 6449 7901 7987 7999 8345 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1986 1977 1452 642 444 385 377 367 346 229 86 30 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2071 2762 4113 4287 4509 4680 Size: 552 939 67 691 1351 174 222 171 Cuts at: 4680 513 Size: 4183 Fragments arranged by size: 4183 1351 939 691 552 222 174 171 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2143 3298 4050 4291 487 Size: 683 56 917 1155 752 241 4546 Fragments arranged by size: 4546 1155 917 752 683 241 56 BtsI GCAGTG_nn' Cuts at: 2865 3050 5596 5866 6660 6680 2865 Size: 185 2546 270 794 20 4535 Fragments arranged by size: 4535 2546 794 270 185 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8315 DraI TTT'AAA Cuts at: 5654 5654 Size: 8350 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2407 2467 4133 5027 7357 809 Size: 507 1091 60 1666 894 2330 1802 Fragments arranged by size: 2330 1802 1666 1091 894 507 60 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2634 3304 3336 Size: 54 1425 50 275 18 804 670 32 Cuts at: 3336 3411 3971 5037 5123 5640 5797 6686 8315 Size: 75 560 1066 86 517 157 889 1629 Cuts at: 8315 8 Size: 43 Fragments arranged by size: 1629 1425 1066 889 804 670 560 517 275 157 86 75 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3971 5123 5797 1537 Size: 275 2159 1152 674 4090 Fragments arranged by size: 4090 2159 1152 674 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3987 4517 5616 6280 1353 Size: 2634 530 1099 664 3423 Fragments arranged by size: 3423 2634 1099 664 530 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3998 6937 7311 7457 387 Size: 1064 138 2409 2939 374 146 1280 Fragments arranged by size: 2939 2409 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4059 4690 5086 5400 4059 Size: 631 396 314 7009 Fragments arranged by size: 7009 631 396 314 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2549 2588 2600 3017 3308 3722 Size: 393 533 384 39 12 417 291 414 Cuts at: 3722 3743 4205 4235 4860 5069 5287 5299 5396 Size: 21 462 30 625 209 218 12 97 Cuts at: 5396 6413 7010 7797 8065 1239 Size: 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 787 625 597 533 462 417 414 393 384 291 268 218 209 97 39 30 21 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 2488 2488 Size: 8350 EcoO109I rG'GnC_Cy Cuts at: 2584 2713 3031 3082 4612 5247 2584 Size: 129 318 51 1530 635 5687 Fragments arranged by size: 5687 1530 635 318 129 51 EcoRI G'AATT_C Cuts at: 5659 6037 5659 Size: 378 7972 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2287 5317 2287 Size: 3030 5320 FspI TGC'GCA Cuts at: 6856 6856 Size: 8350 HaeII r_GCGC'y Cuts at: 1678 2901 3642 3798 4009 4270 7497 1678 Size: 1223 741 156 211 261 3227 2531 Fragments arranged by size: 3227 2531 1223 741 261 211 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2202 2234 2329 2361 3704 Size: 32 135 32 630 32 95 32 1343 Cuts at: 3704 3736 3893 3925 4778 4810 5326 5358 6991 Size: 32 157 32 853 32 516 32 1633 Cuts at: 6991 7023 7065 7097 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1633 1343 853 630 516 157 135 95 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5578 5694 5940 669 Size: 988 281 3640 116 246 3079 Fragments arranged by size: 3640 3079 988 281 246 116 HindIII A'AGCT_T Cuts at: 748 3755 748 Size: 3007 5343 HpaI GTT'AAC Cuts at: 5940 5940 Size: 8350 KasI G'GCGC_C Cuts at: 4005 4005 Size: 8350 KpnI G_GTAC'C Cuts at: 3174 4084 5675 3174 Size: 910 1591 5849 Fragments arranged by size: 5849 1591 910 MfeI C'AATT_G Cuts at: 5949 5949 Size: 8350 MluI A'CGCG_T Cuts at: 8226 8226 Size: 8350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3249 3957 5211 7464 7648 1305 Size: 1944 708 1254 2253 184 2007 Fragments arranged by size: 2253 2007 1944 1254 708 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2636 3338 3413 5039 5642 Size: 54 1425 343 804 702 75 1626 603 Cuts at: 5642 10 Size: 2718 Fragments arranged by size: 2718 1626 1425 804 702 603 343 75 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2246 3269 3344 4112 5225 5423 Size: 648 996 84 1023 75 768 1113 198 Cuts at: 5423 6308 6667 6826 518 Size: 885 359 159 2042 Fragments arranged by size: 2042 1113 1023 996 885 768 648 359 198 159 84 75 MspA1I CmG'CkG Cuts at: 1606 2073 2223 2764 3427 3736 3955 4895 5170 Size: 467 150 541 663 309 219 940 275 Cuts at: 5170 5319 5766 6443 7591 7836 1606 Size: 149 447 677 1148 245 2120 Fragments arranged by size: 2120 1148 940 677 663 541 467 447 309 275 245 219 150 149 NarI GG'CG_CC Cuts at: 4006 4006 Size: 8350 NcoI C'CATG_G Cuts at: 513 1065 2004 4509 513 Size: 552 939 2505 4354 Fragments arranged by size: 4354 2505 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8350 NotI GC'GGCC_GC Cuts at: 5797 5797 Size: 8350 NspI r_CATG'y Cuts at: 1132 1229 2044 2887 5708 7253 1132 Size: 97 815 843 2821 1545 2229 Fragments arranged by size: 2821 2229 1545 843 815 97 PasI CC'CwG_GG Cuts at: 1348 3927 1348 Size: 2579 5771 PciI A'CATG_T Cuts at: 7249 7249 Size: 8350 PflMI CCAn_nnn'nTGG Cuts at: 1108 2709 3328 3511 3661 4324 1108 Size: 1601 619 183 150 663 5134 Fragments arranged by size: 5134 1601 663 619 183 150 PmeI GTTT'AAAC Cuts at: 5654 5654 Size: 8350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5555 5587 6419 6451 7932 7964 5555 Size: 32 832 32 1481 32 5941 Fragments arranged by size: 5941 1481 832 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2584 2713 3031 4612 5247 2584 Size: 129 318 1581 635 5687 Fragments arranged by size: 5687 1581 635 318 129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8350 PsiI TTA'TAA Cuts at: 5920 5920 Size: 8350 PspOMI G'GGCC_C Cuts at: 3081 3081 Size: 8350 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2507 2539 2948 2980 2507 Size: 32 409 32 7877 Fragments arranged by size: 7877 409 32 32 PstI C_TGCA'G Cuts at: 830 2061 3045 3218 3495 3609 3735 5016 5324 Size: 1231 984 173 277 114 126 1281 308 Cuts at: 5324 5702 830 Size: 378 3478 Fragments arranged by size: 3478 1281 1231 984 378 308 277 173 126 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8350 PvuII CAG'CTG Cuts at: 2223 3736 5170 5319 2223 Size: 1513 1434 149 5254 Fragments arranged by size: 5254 1513 1434 149 SacI G_AGCT'C Cuts at: 4061 4692 5088 5402 4061 Size: 631 396 314 7009 Fragments arranged by size: 7009 631 396 314 SacII CC_GC'GG Cuts at: 2074 2765 2074 Size: 691 7659 SalI G'TCGA_C Cuts at: 1936 5576 5692 1936 Size: 3640 116 4594 Fragments arranged by size: 4594 3640 116 SanDI GG'GwC_CC Cuts at: 5247 5247 Size: 8350 SapI GCTCTTCn'nnn_ Cuts at: 3987 4517 3987 Size: 530 7820 SbfI CC_TGCA'GG Cuts at: 5702 5702 Size: 8350 ScaI AGT'ACT Cuts at: 6598 8310 6598 Size: 1712 6638 SfcI C'TryA_G Cuts at: 826 1045 2057 3041 3214 3491 3605 3731 4399 Size: 219 1012 984 173 277 114 126 668 Cuts at: 4399 5012 5320 5350 5698 6833 7514 7705 826 Size: 613 308 30 348 1135 681 191 1471 Fragments arranged by size: 1471 1135 1012 984 681 668 613 348 308 277 219 191 173 126 114 30 SfiI GGCCn_nnn'nGGCC Cuts at: 3833 3833 Size: 8350 SfoI GGC'GCC Cuts at: 4007 4007 Size: 8350 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8350 SmaI CCC'GGG Cuts at: 1872 2596 4095 5677 1872 Size: 724 1499 1582 4545 Fragments arranged by size: 4545 1582 1499 724 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 2362 5072 5240 5389 6454 Size: 197 926 69 350 2710 168 149 1065 Cuts at: 6454 7355 7617 820 Size: 901 262 1553 Fragments arranged by size: 2710 1553 1065 926 901 350 262 197 168 149 69 SnaBI TAC'GTA Cuts at: 493 6141 493 Size: 5648 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8350 SphI G_CATG'C Cuts at: 1132 2887 5708 1132 Size: 1755 2821 3774 Fragments arranged by size: 3774 2821 1755 SspI AAT'ATT Cuts at: 5 52 6274 5 Size: 47 6222 2081 Fragments arranged by size: 6222 2081 47 StuI AGG'CCT Cuts at: 1517 3864 1517 Size: 2347 6003 StyI C'CwwG_G Cuts at: 513 1065 2004 3765 3831 4509 5789 513 Size: 552 939 1761 66 678 1280 3074 Fragments arranged by size: 3074 1761 1280 939 678 552 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3437 3668 6377 6394 6547 6732 7177 Size: 1255 1857 231 2709 17 153 185 445 Cuts at: 7177 325 Size: 1498 Fragments arranged by size: 2709 1857 1498 1255 445 231 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 3234 5508 Size: 274 80 33 51 1443 537 720 2274 Cuts at: 5508 6596 8308 96 Size: 1088 1712 138 Fragments arranged by size: 2274 1712 1443 1088 720 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2996 3028 5456 5488 5575 5607 8310 8342 2996 Size: 32 2428 32 87 32 2703 32 3004 Fragments arranged by size: 3004 2703 2428 87 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2066 4331 5251 1316 Size: 750 2265 920 4415 Fragments arranged by size: 4415 2265 920 750 XbaI T'CTAG_A Cuts at: 5686 5686 Size: 8350 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8350 XmaI C'CCGG_G Cuts at: 1870 2594 4093 5675 1870 Size: 724 1499 1582 4545 Fragments arranged by size: 4545 1582 1499 724 XmnI GAAnn'nnTTC Cuts at: 6479 6479 Size: 8350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2326 2694 6158 276 Size: 53 83 186 936 792 368 3464 2468 Fragments arranged by size: 3464 2468 936 792 368 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SexAI SrfI SwaI XcmI