(Circular) (Six-base) MAPSORT of: pFN21AB3031.seq Check: 110 from: 1 to: 6484 pFN21AB3031 6484 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4547 5931 6199 1632 Size: 2915 1384 268 1917 Fragments arranged by size: 2915 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 5383 6360 1203 Size: 4180 977 1327 Fragments arranged by size: 4180 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 2494 5213 2494 Size: 2719 3765 AleI CACnn'nnGTG Cuts at: 1166 2042 2886 3381 1166 Size: 876 844 495 4269 Fragments arranged by size: 4269 876 844 495 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3399 1184 Size: 2215 4269 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 2430 Size: 32 6452 AlwNI CAG_nnn'CTG Cuts at: 2533 4227 5799 2533 Size: 1694 1572 3218 Fragments arranged by size: 3218 1694 1572 ApaLI G'TGCA_C Cuts at: 3329 3374 4541 5697 3329 Size: 45 1167 1156 4116 Fragments arranged by size: 4116 1167 1156 45 ApoI r'AATT_y Cuts at: 1493 2213 2753 2942 3793 4019 4171 6280 1493 Size: 720 540 189 851 226 152 2109 1697 Fragments arranged by size: 2109 1697 851 720 540 226 189 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 3054 3809 1870 Size: 73 1111 755 4545 Fragments arranged by size: 4545 1111 755 73 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3805 5260 618 Size: 325 217 627 2018 1455 1842 Fragments arranged by size: 2018 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3680 3803 1391 Size: 2289 123 4072 Fragments arranged by size: 4072 2289 123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3732 928 Size: 971 1833 3680 Fragments arranged by size: 3680 1833 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 2860 1548 Size: 1312 5172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2225 5095 136 Size: 107 122 71 631 605 553 2870 1525 Fragments arranged by size: 2870 1525 631 605 553 122 107 71 BglII A'GATC_T Cuts at: 2578 6479 2578 Size: 3901 2583 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 3291 3333 3378 3624 4545 5701 1790 Size: 1501 42 45 246 921 1156 2573 Fragments arranged by size: 2573 1501 1156 921 246 45 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4215 5173 442 Size: 416 53 83 399 24 2798 958 1753 Fragments arranged by size: 2798 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2139 2457 3341 5144 1239 Size: 900 318 884 1803 2579 Fragments arranged by size: 2579 1803 900 884 318 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2624 4609 5474 5772 2624 Size: 1985 865 298 3336 Fragments arranged by size: 3336 1985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2711 3504 5147 882 Size: 617 1212 793 1643 2219 Fragments arranged by size: 2219 1643 1212 793 617 BsaAI yAC'GTr Cuts at: 493 1204 4275 493 Size: 711 3071 2702 Fragments arranged by size: 3071 2702 711 BsaBI GATnn'nnATC Cuts at: 2889 6478 2889 Size: 3589 2895 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3147 4291 4673 5308 Size: 53 83 186 936 1614 1144 382 635 Cuts at: 5308 275 Size: 1451 Fragments arranged by size: 1614 1451 1144 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4917 5315 5589 5736 6147 4917 Size: 398 274 147 411 5254 Fragments arranged by size: 5254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2430 2460 1178 Size: 30 165 30 1027 30 5202 Fragments arranged by size: 5202 1027 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2088 2568 3338 1186 Size: 902 480 770 4332 Fragments arranged by size: 4332 902 770 480 BseYI C'CCAG_C Cuts at: 3290 5687 6183 3290 Size: 2397 496 3591 Fragments arranged by size: 3591 2397 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2337 3732 1163 Size: 573 601 1395 3915 Fragments arranged by size: 3915 1395 601 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3369 3934 4695 5723 1540 Size: 275 186 1368 565 761 1028 2301 Fragments arranged by size: 2301 1368 1028 761 565 275 186 BsiHKAI G_wGCw'C Cuts at: 3333 3378 4545 4630 5701 3333 Size: 45 1167 85 1071 4116 Fragments arranged by size: 4116 1167 1071 85 45 BsmI GAATG_Cn' Cuts at: 3995 4088 3995 Size: 93 6391 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3291 3333 3378 3624 3680 3803 4545 Size: 399 1501 42 45 246 56 123 742 Cuts at: 4545 4630 5701 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1501 1071 742 399 246 123 85 56 45 42 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 3066 3184 4373 6103 3066 Size: 118 1189 1730 3447 Fragments arranged by size: 3447 1730 1189 118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3839 844 Size: 2995 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3182 4371 1394 Size: 429 1359 1189 3507 Fragments arranged by size: 3507 1359 1189 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2491 2985 4979 5153 65 Size: 1183 1243 494 1994 174 1396 Fragments arranged by size: 1994 1396 1243 1183 494 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3364 5128 5315 1700 Size: 110 1554 1764 187 2869 Fragments arranged by size: 2869 1764 1554 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 3490 3665 3490 Size: 175 6309 BstYI r'GATC_y Cuts at: 1972 2320 2578 3814 4181 4566 4583 6035 6121 Size: 348 258 1236 367 385 17 1452 86 Cuts at: 6121 6133 6479 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1236 385 367 348 346 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3280 513 Size: 552 2215 3717 Fragments arranged by size: 3717 2215 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 1995 487 Size: 683 56 769 4976 Fragments arranged by size: 4976 769 683 56 BtsI GCAGTG_nn' Cuts at: 3376 3388 4000 4794 4814 3376 Size: 12 612 794 20 5046 Fragments arranged by size: 5046 794 612 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 3286 3710 3286 Size: 424 6060 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3272 3394 3931 Size: 54 1425 50 275 18 1442 122 537 Cuts at: 3931 4820 6449 8 Size: 889 1629 43 Fragments arranged by size: 1629 1442 1425 889 537 275 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3931 1537 Size: 275 2119 4090 Fragments arranged by size: 4090 2119 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3591 4414 1353 Size: 2238 823 3423 Fragments arranged by size: 3423 2238 823 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3046 5071 5445 5591 387 Size: 1064 138 1457 2025 374 146 1280 Fragments arranged by size: 2025 1457 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2139 2457 3341 4547 5144 5931 6199 Size: 393 507 318 884 1206 597 787 268 Cuts at: 6199 1239 Size: 1524 Fragments arranged by size: 1524 1206 884 787 597 507 393 318 268 EcoNI CCTnn'n_nnAGG Cuts at: 2332 3323 2332 Size: 991 5493 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FspI TGC'GCA Cuts at: 2459 4990 2459 Size: 2531 3953 FspAI rTGC'GCAy Cuts at: 2459 2459 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 3180 5631 1678 Size: 1502 2451 2531 Fragments arranged by size: 2531 2451 1502 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2312 2344 2480 2509 2512 Size: 32 135 32 740 32 136 29 3 Cuts at: 2512 2541 3048 3080 3655 3687 5125 5157 5199 Size: 29 507 32 575 32 1438 32 42 Cuts at: 5199 5231 1373 Size: 32 2626 Fragments arranged by size: 2626 1438 740 575 507 136 135 42 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2067 3457 3828 4074 669 Size: 988 281 129 1390 371 246 3079 Fragments arranged by size: 3079 1390 988 371 281 246 129 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3202 3722 5598 5782 1305 Size: 1897 520 1876 184 2007 Fragments arranged by size: 2007 1897 1876 520 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4662 Fragments arranged by size: 4662 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2886 3381 3488 4442 4801 4960 Size: 648 876 844 495 107 954 359 159 Cuts at: 4960 518 Size: 2042 Fragments arranged by size: 2042 954 876 844 648 495 359 159 107 MspA1I CmG'CkG Cuts at: 1606 3900 4577 5725 5970 1606 Size: 2294 677 1148 245 2120 Fragments arranged by size: 2294 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5932 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 3842 5387 1132 Size: 97 2613 1545 2229 Fragments arranged by size: 2613 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3299 1348 Size: 1951 4533 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6484 PfoI T'CCnGG_A Cuts at: 3318 3318 Size: 6484 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2430 2462 4553 4585 6066 6098 2430 Size: 32 2091 32 1481 32 2816 Fragments arranged by size: 2816 2091 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 2595 4054 2595 Size: 1459 5025 PstI C_TGCA'G Cuts at: 830 3383 3836 830 Size: 2553 453 3478 Fragments arranged by size: 3478 2553 453 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SapI GCTCTTCn'nnn_ Cuts at: 3591 3591 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 2631 4732 6444 2631 Size: 2101 1712 2671 Fragments arranged by size: 2671 2101 1712 SfcI C'TryA_G Cuts at: 826 1045 2791 3379 3578 3832 4967 5648 5839 Size: 219 1746 588 199 254 1135 681 191 Cuts at: 5839 826 Size: 1471 Fragments arranged by size: 1746 1471 1135 681 588 254 219 199 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 4588 5489 5751 820 Size: 197 926 696 1949 901 262 1553 Fragments arranged by size: 1949 1553 926 901 696 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SspI AAT'ATT Cuts at: 5 52 3637 4408 5 Size: 47 3585 771 2081 Fragments arranged by size: 3585 2081 771 47 StuI AGG'CCT Cuts at: 1517 2101 1517 Size: 584 5900 StyI C'CwwG_G Cuts at: 513 1065 3923 513 Size: 552 2858 3074 Fragments arranged by size: 3074 2858 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2043 2174 4511 4528 4681 4866 5311 Size: 1255 463 131 2337 17 153 185 445 Cuts at: 5311 325 Size: 1498 Fragments arranged by size: 2337 1498 1255 463 445 185 153 131 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2629 3692 4730 Size: 274 80 33 51 1443 652 1063 1038 Cuts at: 4730 6442 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1063 1038 652 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6444 6476 6444 Size: 32 6452 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2432 2432 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 3776 4613 3776 Size: 837 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI