(Circular) (Six-base) MAPSORT of: pFN21AB3027.seq Check: 9341 from: 1 to: 8347 pFN21AB3027 8347 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3167 3702 4350 3167 Size: 535 648 7164 Fragments arranged by size: 7164 648 535 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6157 278 Size: 53 83 186 936 4621 2468 Fragments arranged by size: 4621 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2097 4965 5690 1937 Size: 160 2868 725 4594 Fragments arranged by size: 4594 2868 725 160 Acc65I G'GTAC_C Cuts at: 3988 5668 3988 Size: 1680 6667 AclI AA'CG_TT Cuts at: 1563 6474 6847 1563 Size: 4911 373 3063 Fragments arranged by size: 4911 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3662 3946 4311 4909 5025 6410 7794 8062 Size: 2030 284 365 598 116 1385 1384 268 Cuts at: 8062 1632 Size: 1917 Fragments arranged by size: 2030 1917 1385 1384 598 365 284 268 116 AfeI AGC'GCT Cuts at: 5035 5035 Size: 8347 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8150 AflIII A'CryG_T Cuts at: 1203 2886 4635 7246 8223 1203 Size: 1683 1749 2611 977 1327 Fragments arranged by size: 2611 1749 1683 1327 977 AgeI A'CCGG_T Cuts at: 2601 7178 2601 Size: 4577 3770 AhdI GACnn_n'nnGTC Cuts at: 5008 7076 5008 Size: 2068 6279 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4197 5624 1184 Size: 3013 1427 3907 Fragments arranged by size: 3907 3013 1427 AlwNI CAG_nnn'CTG Cuts at: 2950 3530 3622 3633 4113 5006 5141 5473 6090 Size: 580 92 11 480 893 135 332 617 Cuts at: 6090 7662 2950 Size: 1572 3635 Fragments arranged by size: 3635 1572 893 617 580 480 332 135 92 11 ApaI G_GGCC'C Cuts at: 3052 3754 4794 3052 Size: 702 1040 6605 Fragments arranged by size: 6605 1040 702 ApaLI G'TGCA_C Cuts at: 2903 6404 7560 2903 Size: 3501 1156 3690 Fragments arranged by size: 3690 3501 1156 ApoI r'AATT_y Cuts at: 1493 4069 5656 5882 6034 8143 1493 Size: 2576 1587 226 152 2109 1697 Fragments arranged by size: 2576 2109 1697 1587 226 152 AseI AT'TA_AT Cuts at: 160 1032 6901 160 Size: 872 5869 1606 Fragments arranged by size: 5869 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8347 AvaI C'yCGr_G Cuts at: 1870 1943 2381 4522 5097 5331 5418 5672 1870 Size: 73 438 2141 575 234 87 254 4545 Fragments arranged by size: 4545 2141 575 438 254 234 87 73 BamHI G'GATC_C Cuts at: 3072 5677 6044 3072 Size: 2605 367 5375 Fragments arranged by size: 5375 2605 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2199 3017 3293 3302 3988 Size: 325 217 627 412 818 276 9 686 Cuts at: 3988 4203 5668 7123 618 Size: 215 1465 1455 1842 Fragments arranged by size: 1842 1465 1455 818 686 627 412 325 276 217 215 9 BanII G_rGCy'C Cuts at: 1391 3052 3185 3443 3754 3808 4794 4847 4865 Size: 1661 133 258 311 54 986 53 18 Cuts at: 4865 5590 5666 1391 Size: 725 76 4072 Fragments arranged by size: 4072 1661 986 725 311 258 133 76 54 53 18 BbeI G_GCGC'C Cuts at: 2203 3297 4207 2203 Size: 1094 910 6343 Fragments arranged by size: 6343 1094 910 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2033 2708 3094 4709 4899 928 Size: 971 134 675 386 1615 190 4376 Fragments arranged by size: 4376 1615 971 675 386 190 134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2600 2634 2786 2820 4376 4410 6538 Size: 34 663 34 152 34 1556 34 2128 Cuts at: 6538 6572 1903 Size: 34 3678 Fragments arranged by size: 3678 2128 1556 663 152 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5122 6239 7449 5122 Size: 1117 1210 6020 Fragments arranged by size: 6020 1210 1117 BclI T'GATC_A Cuts at: 1548 4437 5310 1548 Size: 2889 873 4585 Fragments arranged by size: 4585 2889 873 BfrBI ATG'CAT Cuts at: 4246 4246 Size: 8347 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3251 4723 4732 Size: 107 122 71 631 605 1579 1472 9 Cuts at: 4732 4776 6958 136 Size: 44 2182 1525 Fragments arranged by size: 2182 1579 1525 1472 631 605 122 107 71 44 9 BglII A'GATC_T Cuts at: 2733 8342 2733 Size: 5609 2738 BlpI GC'TnA_GC Cuts at: 5764 5764 Size: 8347 Bme1580I G_kGCm'C Cuts at: 1790 2075 2907 2998 3005 3052 3546 3754 4794 Size: 285 832 91 7 47 494 208 1040 Cuts at: 4794 5105 5168 5373 6408 7564 1790 Size: 311 63 205 1035 1156 2573 Fragments arranged by size: 2573 1156 1040 1035 832 494 311 285 208 205 91 63 47 7 BmgBI CAC'GTC Cuts at: 1318 4496 1318 Size: 3178 5169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3757 4848 6078 Size: 416 53 83 399 24 2340 1091 1230 Cuts at: 6078 7036 442 Size: 958 1753 Fragments arranged by size: 2340 1753 1230 1091 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8347 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2538 5149 5173 5444 2538 Size: 2611 24 271 5441 Fragments arranged by size: 5441 2611 271 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2780 3336 3457 4287 4346 4394 5399 5555 Size: 1541 556 121 830 59 48 1005 156 Cuts at: 5555 7007 1239 Size: 1452 2579 Fragments arranged by size: 2579 1541 1452 1005 830 556 156 121 59 48 Bpu10I CC'TnA_GC Cuts at: 3184 4506 3184 Size: 1322 7025 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3752 4103 6472 7337 7635 3752 Size: 351 2369 865 298 4464 Fragments arranged by size: 4464 2369 865 351 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2075 2251 5624 7010 882 Size: 617 576 176 3373 1386 2219 Fragments arranged by size: 3373 2219 1386 617 576 176 BsaAI yAC'GTr Cuts at: 493 1204 6138 493 Size: 711 4934 2702 Fragments arranged by size: 4934 2702 711 BsaBI GATnn'nnATC Cuts at: 8341 8341 Size: 8347 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2200 3294 4204 6154 Size: 53 83 186 936 667 1094 910 1950 Cuts at: 6154 6536 7171 275 Size: 382 635 1451 Fragments arranged by size: 1950 1451 1094 936 910 667 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2601 5046 6780 7178 7452 7599 8010 2601 Size: 2445 1734 398 274 147 411 2938 Fragments arranged by size: 2938 2445 1734 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 1178 Size: 30 165 30 1350 30 6742 Fragments arranged by size: 6742 1350 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 2639 2675 3413 3428 3431 4100 4607 Size: 1213 240 36 738 15 3 669 507 Cuts at: 4607 1186 Size: 4926 Fragments arranged by size: 4926 1213 738 669 507 240 36 15 3 BseYI C'CCAG_C Cuts at: 2212 2231 2289 3045 3246 4766 4797 5302 7550 Size: 19 58 756 201 1520 31 505 2248 Cuts at: 7550 8046 2212 Size: 496 2513 Fragments arranged by size: 2513 2248 1520 756 505 496 201 58 31 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3608 1163 Size: 573 1872 5902 Fragments arranged by size: 5902 1872 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5797 6558 7586 1540 Size: 275 186 3796 761 1028 2301 Fragments arranged by size: 3796 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2519 2907 3132 3443 3808 4191 4865 6408 6493 Size: 388 225 311 365 383 674 1543 85 Cuts at: 6493 7564 2519 Size: 1071 3302 Fragments arranged by size: 3302 1543 1071 674 388 383 365 311 225 85 BsmI GAATG_Cn' Cuts at: 4399 5858 5951 4399 Size: 1459 93 6795 Fragments arranged by size: 6795 1459 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3848 4229 4481 3848 Size: 381 252 7714 Fragments arranged by size: 7714 381 252 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2519 2907 2998 3005 3052 3132 Size: 399 285 444 388 91 7 47 80 Cuts at: 3132 3185 3443 3546 3754 3808 4191 4794 4847 Size: 53 258 103 208 54 383 603 53 Cuts at: 4847 4865 5105 5168 5373 5590 5666 6408 6493 Size: 18 240 63 205 217 76 742 85 Cuts at: 6493 7564 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 603 444 399 388 383 285 258 240 217 208 205 103 91 85 80 76 63 54 53 53 47 18 7 BspEI T'CCGG_A Cuts at: 8010 8010 Size: 8347 BspHI T'CATG_A Cuts at: 2685 4809 6236 7966 2685 Size: 2124 1427 1730 3066 Fragments arranged by size: 3066 2124 1730 1427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2093 3167 3702 4350 5702 844 Size: 1249 1074 535 648 1352 3489 Fragments arranged by size: 3489 1352 1249 1074 648 535 BsrBI CCG'CTC Cuts at: 1394 1823 4312 4378 4419 4510 6234 1394 Size: 429 2489 66 41 91 1724 3507 Fragments arranged by size: 3507 2489 1724 429 91 66 41 BsrDI GCAATG_nn' Cuts at: 65 1248 2848 2872 3668 6842 7016 65 Size: 1183 1600 24 796 3174 174 1396 Fragments arranged by size: 3174 1600 1396 1183 796 174 24 BsrFI r'CCGG_y Cuts at: 1700 1810 2601 3970 4731 6991 7178 1700 Size: 110 791 1369 761 2260 187 2869 Fragments arranged by size: 2869 2260 1369 791 761 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8347 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8347 BssSI C'ACGA_G Cuts at: 2979 3815 5267 6407 7419 2979 Size: 836 1452 1140 1012 3907 Fragments arranged by size: 3907 1452 1140 1012 836 BstAPI GCAn_nnn'nTGC Cuts at: 794 3201 3622 5473 794 Size: 2407 421 1851 3668 Fragments arranged by size: 3668 2407 1851 421 BstBI TT'CG_AA Cuts at: 7151 7151 Size: 8347 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8347 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3147 3254 2011 Size: 1136 107 7104 Fragments arranged by size: 7104 1136 107 BstYI r'GATC_y Cuts at: 1972 2733 3072 3501 5677 6044 6429 6446 7898 Size: 761 339 429 2176 367 385 17 1452 Cuts at: 7898 7984 7996 8342 1972 Size: 86 12 346 1977 Fragments arranged by size: 2176 1977 1452 761 429 385 367 346 339 86 17 12 BstZ17I GTA'TAC Cuts at: 4966 4966 Size: 8347 Bsu36I CC'TnA_GG Cuts at: 2937 3078 5134 2937 Size: 141 2056 6150 Fragments arranged by size: 6150 2056 141 BtgI C'CryG_G Cuts at: 513 1065 2004 3879 4020 4771 5160 5173 5350 Size: 552 939 1875 141 751 389 13 177 Cuts at: 5350 513 Size: 3510 Fragments arranged by size: 3510 1875 939 751 552 389 177 141 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7608 Fragments arranged by size: 7608 683 56 BtsI GCAGTG_nn' Cuts at: 4164 4457 5128 5474 5632 5863 6657 6677 4164 Size: 293 671 346 158 231 794 20 5834 Fragments arranged by size: 5834 794 671 346 293 231 158 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8312 DraI TTT'AAA Cuts at: 5651 5651 Size: 8347 DraIII CAC_nnn'GTG Cuts at: 4427 4427 Size: 8347 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7354 809 Size: 507 6038 1802 Fragments arranged by size: 6038 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2317 2585 2898 Size: 54 1425 50 275 18 487 268 313 Cuts at: 2898 5170 5794 6683 8312 8 Size: 2272 624 889 1629 43 Fragments arranged by size: 2272 1629 1425 889 624 487 313 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5794 1537 Size: 275 3982 4090 Fragments arranged by size: 4090 3982 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2882 3596 4302 4946 5195 5447 6277 1353 Size: 1529 714 706 644 249 252 830 3423 Fragments arranged by size: 3423 1529 830 714 706 644 252 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4181 4480 6934 7308 7454 387 Size: 1064 138 2592 299 2454 374 146 1280 Fragments arranged by size: 2592 2454 1280 1064 374 299 146 138 EcoICRI GAG'CTC Cuts at: 3441 3806 4863 3441 Size: 365 1057 6925 Fragments arranged by size: 6925 1057 365 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2780 3336 3457 3662 3946 4287 4311 Size: 393 1148 556 121 205 284 341 24 Cuts at: 4311 4346 4394 4909 5025 5399 5555 6410 7007 Size: 35 48 515 116 374 156 855 597 Cuts at: 7007 7794 8062 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1148 855 787 597 556 515 393 374 341 284 268 205 156 121 116 48 35 24 EcoNI CCTnn'n_nnAGG Cuts at: 5519 5581 5519 Size: 62 8285 EcoO109I rG'GnC_Cy Cuts at: 2389 3189 3664 3751 4628 4790 4791 5512 2389 Size: 800 475 87 877 162 1 721 5224 Fragments arranged by size: 5224 877 800 721 475 162 87 1 EcoRI G'AATT_C Cuts at: 5656 6034 5656 Size: 378 7969 EcoRV GAT'ATC Cuts at: 2683 2683 Size: 8347 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4063 4975 4063 Size: 912 7435 FseI GG_CCGG'CC Cuts at: 4735 4735 Size: 8347 FspI TGC'GCA Cuts at: 6853 6853 Size: 8347 HaeII r_GCGC'y Cuts at: 1678 2203 3046 3297 4207 5037 7494 1678 Size: 525 843 251 910 830 2457 2531 Fragments arranged by size: 2531 2457 910 843 830 525 251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2753 2785 4994 Size: 32 135 32 773 32 376 32 2209 Cuts at: 4994 5026 5228 5260 5302 5334 6988 7020 7062 Size: 32 202 32 42 32 1654 32 42 Cuts at: 7062 7094 1373 Size: 32 2626 Fragments arranged by size: 2626 2209 1654 773 376 202 135 42 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5691 5937 669 Size: 988 281 3753 246 3079 Fragments arranged by size: 3753 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4469 748 Size: 3721 4626 HpaI GTT'AAC Cuts at: 5937 5937 Size: 8347 KasI G'GCGC_C Cuts at: 2199 3293 4203 2199 Size: 1094 910 6343 Fragments arranged by size: 6343 1094 910 KpnI G_GTAC'C Cuts at: 3992 5672 3992 Size: 1680 6667 MfeI C'AATT_G Cuts at: 5946 5946 Size: 8347 MluI A'CGCG_T Cuts at: 8223 8223 Size: 8347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2508 2678 5574 7461 7645 1305 Size: 1203 170 2896 1887 184 2007 Fragments arranged by size: 2896 2007 1887 1203 184 170 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6525 Fragments arranged by size: 6525 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2068 2502 3269 3884 5366 6305 Size: 648 843 59 434 767 615 1482 939 Cuts at: 6305 6664 6823 518 Size: 359 159 2042 Fragments arranged by size: 2042 1482 939 843 767 648 615 434 359 159 59 MspA1I CmG'CkG Cuts at: 1606 2432 3781 3892 4709 4929 5175 5251 5763 Size: 826 1349 111 817 220 246 76 512 Cuts at: 5763 6440 7588 7833 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1349 1148 826 817 677 512 246 245 220 111 76 NaeI GCC'GGC Cuts at: 4733 4733 Size: 8347 NarI GG'CG_CC Cuts at: 2200 3294 4204 2200 Size: 1094 910 6343 Fragments arranged by size: 6343 1094 910 NcoI C'CATG_G Cuts at: 513 1065 2004 3879 4020 5350 513 Size: 552 939 1875 141 1330 3510 Fragments arranged by size: 3510 1875 1330 939 552 141 NdeI CA'TA_TG Cuts at: 387 387 Size: 8347 NgoMIV G'CCGG_C Cuts at: 4731 4731 Size: 8347 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8347 NotI GC'GGCC_GC Cuts at: 5794 5794 Size: 8347 NsiI A_TGCA'T Cuts at: 4248 4248 Size: 8347 NspI r_CATG'y Cuts at: 1132 1229 2890 3243 4246 4639 5705 7250 1132 Size: 97 1661 353 1003 393 1066 1545 2229 Fragments arranged by size: 2229 1661 1545 1066 1003 393 353 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8347 PciI A'CATG_T Cuts at: 2886 4635 7246 2886 Size: 1749 2611 3987 Fragments arranged by size: 3987 2611 1749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2296 4261 4919 5191 1108 Size: 1188 1965 658 272 4264 Fragments arranged by size: 4264 1965 1188 658 272 PfoI T'CCnGG_A Cuts at: 3473 3473 Size: 8347 PmeI GTTT'AAAC Cuts at: 5651 5651 Size: 8347 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6416 6448 7929 7961 6416 Size: 32 1481 32 6802 Fragments arranged by size: 6802 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3664 4628 5512 3664 Size: 964 884 6499 Fragments arranged by size: 6499 964 884 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8347 PsiI TTA'TAA Cuts at: 5917 5917 Size: 8347 PspOMI G'GGCC_C Cuts at: 3048 3750 4790 3048 Size: 702 1040 6605 Fragments arranged by size: 6605 1040 702 PspXI vC'TCGA_Gb Cuts at: 4522 4522 Size: 8347 PstI C_TGCA'G Cuts at: 830 2623 2970 3687 4982 5595 5699 830 Size: 1793 347 717 1295 613 104 3478 Fragments arranged by size: 3478 1793 1295 717 613 347 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8347 PvuII CAG'CTG Cuts at: 2432 3892 4709 2432 Size: 1460 817 6070 Fragments arranged by size: 6070 1460 817 SacI G_AGCT'C Cuts at: 3443 3808 4865 3443 Size: 365 1057 6925 Fragments arranged by size: 6925 1057 365 SacII CC_GC'GG Cuts at: 5176 5176 Size: 8347 SalI G'TCGA_C Cuts at: 1936 5689 1936 Size: 3753 4594 SanDI GG'GwC_CC Cuts at: 3664 4628 3664 Size: 964 7383 SapI GCTCTTCn'nnn_ Cuts at: 3596 4302 3596 Size: 706 7641 SbfI CC_TGCA'GG Cuts at: 5699 5699 Size: 8347 ScaI AGT'ACT Cuts at: 6595 8307 6595 Size: 1712 6635 SfcI C'TryA_G Cuts at: 826 1045 2619 2966 3683 4978 5591 5695 6830 Size: 219 1574 347 717 1295 613 104 1135 Cuts at: 6830 7511 7702 826 Size: 681 191 1471 Fragments arranged by size: 1574 1471 1295 1135 717 681 613 347 219 191 104 SfiI GGCCn_nnn'nGGCC Cuts at: 4732 4732 Size: 8347 SfoI GGC'GCC Cuts at: 2201 3295 4205 2201 Size: 1094 910 6343 Fragments arranged by size: 6343 1094 910 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8347 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8347 SmaI CCC'GGG Cuts at: 1872 5674 1872 Size: 3802 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3767 4118 4522 6451 7352 7614 Size: 197 926 1824 351 404 1929 901 262 Cuts at: 7614 820 Size: 1553 Fragments arranged by size: 1929 1824 1553 926 901 404 351 262 197 SnaBI TAC'GTA Cuts at: 493 6138 493 Size: 5645 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8347 SphI G_CATG'C Cuts at: 1132 4246 5705 1132 Size: 3114 1459 3774 Fragments arranged by size: 3774 3114 1459 SspI AAT'ATT Cuts at: 5 52 6271 5 Size: 47 6219 2081 Fragments arranged by size: 6219 2081 47 StuI AGG'CCT Cuts at: 1517 4623 5153 1517 Size: 3106 530 4711 Fragments arranged by size: 4711 3106 530 StyI C'CwwG_G Cuts at: 513 1065 2004 3462 3879 3924 4020 4131 5350 Size: 552 939 1458 417 45 96 111 1219 Cuts at: 5350 5786 513 Size: 436 3074 Fragments arranged by size: 3074 1458 1219 939 552 436 417 111 96 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 2769 5489 6374 6391 6544 6729 Size: 1255 665 524 2720 885 17 153 185 Cuts at: 6729 7174 325 Size: 445 1498 Fragments arranged by size: 2720 1498 1255 885 665 524 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5566 6593 8305 Size: 274 80 33 51 1443 3589 1027 1712 Cuts at: 8305 96 Size: 138 Fragments arranged by size: 3589 1712 1443 1027 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3486 3518 5255 5287 8307 8339 3486 Size: 32 1737 32 3020 32 3494 Fragments arranged by size: 3494 3020 1737 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8347 XbaI T'CTAG_A Cuts at: 5683 5683 Size: 8347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3144 4262 5485 3144 Size: 1118 1223 6006 Fragments arranged by size: 6006 1223 1118 XhoI C'TCGA_G Cuts at: 1943 4522 1943 Size: 2579 5768 XmaI C'CCGG_G Cuts at: 1870 5672 1870 Size: 3802 4545 XmnI GAAnn'nnTTC Cuts at: 2030 4955 6476 2030 Size: 2925 1521 3901 Fragments arranged by size: 3901 2925 1521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6155 276 Size: 53 83 186 936 4621 2468 Fragments arranged by size: 4621 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BsiWI FspAI NruI PacI PmlI PsrI RsrII SexAI SrfI SwaI