Restriction map for
pFN21AB3004
(Circular) (Six-base) MAPSORT of: pFN21AB3004.seq Check: 7650 from: 1 to: 7855
pFN21AB3004 7855 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5665 278
Size: 53 83 186 936 4129 2468
Fragments arranged by size:
4129 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4857 5198 1937
Size: 2920 341 4594
Fragments arranged by size:
4594 2920 341
Acc65I G'GTAC_C
Cuts at: 5176 5176
Size: 7855
AclI AA'CG_TT
Cuts at: 1563 2303 4444 5982 6355 1563
Size: 740 2141 1538 373 3063
Fragments arranged by size:
3063 2141 1538 740 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2153 3276 3775 4128 4217 4520 5918
Size: 479 42 1123 499 353 89 303 1398
Cuts at: 5918 7302 7570 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1398 1384 1123 499 479 353 303
268 89 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7658
AflIII A'CryG_T
Cuts at: 1203 6754 7731 1203
Size: 5551 977 1327
Fragments arranged by size:
5551 1327 977
AgeI A'CCGG_T
Cuts at: 6686 6686
Size: 7855
AhdI GACnn_n'nnGTC
Cuts at: 6584 6584
Size: 7855
AleI CACnn'nnGTG
Cuts at: 1166 4168 1166
Size: 3002 4853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7855
AlwNI CAG_nnn'CTG
Cuts at: 4195 4810 5033 5598 7170 4195
Size: 615 223 565 1572 4880
Fragments arranged by size:
4880 1572 615 565 223
ApaLI G'TGCA_C
Cuts at: 5912 7068 5912
Size: 1156 6699
ApoI r'AATT_y
Cuts at: 1493 2199 3230 3246 3743 4110 4988 5164 5390
Size: 706 1031 16 497 367 878 176 226
Cuts at: 5390 5542 7651 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1031 878 706 497 367 226
176 152 16
AseI AT'TA_AT
Cuts at: 160 1032 6409 160
Size: 872 5377 1606
Fragments arranged by size:
5377 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7855
AvaI C'yCGr_G
Cuts at: 1870 1943 2598 5180 1870
Size: 73 655 2582 4545
Fragments arranged by size:
4545 2582 655 73
BamHI G'GATC_C
Cuts at: 5185 5552 5185
Size: 367 7488
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4354 4509 5176 6631 618
Size: 325 217 627 2567 155 667 1455 1842
Fragments arranged by size:
2567 1842 1455 667 627 325 217 155
BanII G_rGCy'C
Cuts at: 1391 2725 4016 5174 1391
Size: 1334 1291 1158 4072
Fragments arranged by size:
4072 1334 1291 1158
BbeI G_GCGC'C
Cuts at: 4358 4358
Size: 7855
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2652 3676 3828 4032 4097 4248 4909
Size: 971 753 1024 152 204 65 151 661
Cuts at: 4909 928
Size: 3874
Fragments arranged by size:
3874 1024 971 753 661 204 152 151
65
BbvCI CC'TCA_GC
Cuts at: 4784 4784
Size: 7855
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6046 6080 1903
Size: 34 4109 34 3678
Fragments arranged by size:
4109 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3868 5747 6957 3868
Size: 1879 1210 4766
Fragments arranged by size:
4766 1879 1210
BclI T'GATC_A
Cuts at: 1548 3400 4462 1548
Size: 1852 1062 4941
Fragments arranged by size:
4941 1852 1062
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6466 136
Size: 107 122 71 631 605 337 4457 1525
Fragments arranged by size:
4457 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3931 7850 3931
Size: 3919 3936
BlpI GC'TnA_GC
Cuts at: 4010 5272 4010
Size: 1262 6593
Bme1580I G_kGCm'C
Cuts at: 1790 5916 7072 1790
Size: 4126 1156 2573
Fragments arranged by size:
4126 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7855
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 3570 5586
Size: 416 53 83 399 24 1595 558 2016
Cuts at: 5586 6544 442
Size: 958 1753
Fragments arranged by size:
2016 1753 1595 958 558 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7855
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2765
Size: 7855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 3711 6515 1239
Size: 836 1636 2804 2579
Fragments arranged by size:
2804 2579 1636 836
Bpu10I CC'TnA_GC
Cuts at: 3101 4784 3101
Size: 1683 6172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2259 2648 2735 3784 4415 4672 5980 6845 7143
Size: 389 87 1049 631 257 1308 865 298
Cuts at: 7143 2259
Size: 2971
Fragments arranged by size:
2971 1308 1049 865 631 389 298 257
87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6518 882
Size: 617 5019 2219
Fragments arranged by size:
5019 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5646 493
Size: 711 4442 2702
Fragments arranged by size:
4442 2702 711
BsaBI GATnn'nnATC
Cuts at: 4461 7849 4461
Size: 3388 4467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4355 5662 6044 6679
Size: 53 83 186 936 2822 1307 382 635
Cuts at: 6679 275
Size: 1451
Fragments arranged by size:
2822 1451 1307 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3264 3922 6288 6686 6960 7107 7518 3264
Size: 658 2366 398 274 147 411 3601
Fragments arranged by size:
3601 2366 658 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7630
Fragments arranged by size:
7630 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2657 3188 4039 4081 1186
Size: 1471 531 851 42 4960
Fragments arranged by size:
4960 1471 851 531 42
BseYI C'CCAG_C
Cuts at: 2577 7058 7554 2577
Size: 4481 496 2878
Fragments arranged by size:
4481 2878 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3030 3330 4784 1163
Size: 573 1294 300 1454 4234
Fragments arranged by size:
4234 1454 1294 573 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5305 6066 7094 1540
Size: 275 186 3304 761 1028 2301
Fragments arranged by size:
3304 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2035 2137 2725 2749 5916 6001 7072 2035
Size: 102 588 24 3167 85 1071 2818
Fragments arranged by size:
3167 2818 1071 588 102 85 24
BsmI GAATG_Cn'
Cuts at: 5014 5366 5459 5014
Size: 352 93 7410
Fragments arranged by size:
7410 352 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3407 3407
Size: 7855
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2137 2725 2749 4016 5174 5916
Size: 399 245 102 588 24 1267 1158 742
Cuts at: 5916 6001 7072 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1267 1158 1071 742 588 399 245
102 85 24
BspEI T'CCGG_A
Cuts at: 3264 7518 3264
Size: 4254 3601
BspHI T'CATG_A
Cuts at: 3871 5744 7474 3871
Size: 1873 1730 4252
Fragments arranged by size:
4252 1873 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2943 5210 844
Size: 2099 2267 3489
Fragments arranged by size:
3489 2267 2099
BsrBI CCG'CTC
Cuts at: 1394 1823 5742 1394
Size: 429 3919 3507
Fragments arranged by size:
3919 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2407 6350 6524 65
Size: 1183 1159 3943 174 1396
Fragments arranged by size:
3943 1396 1183 1159 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6499 6686 1700
Size: 110 4689 187 2869
Fragments arranged by size:
4689 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2089 2418 4801 96
Size: 1993 329 2383 3150
Fragments arranged by size:
3150 2383 1993 329
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7855
BssSI C'ACGA_G
Cuts at: 2973 3651 5915 6927 2973
Size: 678 2264 1012 3901
Fragments arranged by size:
3901 2264 1012 678
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7855
BstBI TT'CG_AA
Cuts at: 6659 6659
Size: 7855
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7855
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 2444 2053
Size: 391 7464
BstYI r'GATC_y
Cuts at: 1972 3133 3643 3892 3931 4540 4806 4824 5185
Size: 1161 510 249 39 609 266 18 361
Cuts at: 5185 5552 5937 5954 7406 7492 7504 7850 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1161 609 510 385 367 361
346 266 249 86 39 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 4757 4757
Size: 7855
BtgI C'CryG_G
Cuts at: 513 1065 2004 2885 4166 4409 4642 513
Size: 552 939 881 1281 243 233 3726
Fragments arranged by size:
3726 1281 939 881 552 243 233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7116
Fragments arranged by size:
7116 683 56
BtsI GCAGTG_nn'
Cuts at: 5371 6165 6185 5371
Size: 794 20 7041
Fragments arranged by size:
7041 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7855
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7820
DraI TTT'AAA
Cuts at: 4449 5159 4449
Size: 710 7145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6862 809
Size: 507 5546 1802
Fragments arranged by size:
5546 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5302 6191 7820
Size: 54 1425 50 275 18 3472 889 1629
Cuts at: 7820 8
Size: 43
Fragments arranged by size:
3472 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5302 1537
Size: 275 3490 4090
Fragments arranged by size:
4090 3490 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2634 3033 3311 3339 3405 5785 1353
Size: 819 462 399 278 28 66 2380 3423
Fragments arranged by size:
3423 2380 819 462 399 278 66 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2125 6442 6816 6962 387
Size: 1064 138 536 4317 374 146 1280
Fragments arranged by size:
4317 1280 1064 536 374 146 138
EcoICRI GAG'CTC
Cuts at: 2723 2723
Size: 7855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2111 2153 3276 3711 3775 4128
Size: 393 443 36 42 1123 435 64 353
Cuts at: 4128 4217 4520 5918 6515 7302 7570 1239
Size: 89 303 1398 597 787 268 1524
Fragments arranged by size:
1524 1398 1123 787 597 443 435 393
353 303 268 89 64 42 36
EcoO109I rG'GnC_Cy
Cuts at: 2936 2955 3572 4929 2936
Size: 19 617 1357 5862
Fragments arranged by size:
5862 1357 617 19
EcoRI G'AATT_C
Cuts at: 3230 5164 5542 3230
Size: 1934 378 5543
Fragments arranged by size:
5543 1934 378
FspI TGC'GCA
Cuts at: 6361 6361
Size: 7855
HaeII r_GCGC'y
Cuts at: 1678 4358 7002 1678
Size: 2680 2644 2531
Fragments arranged by size:
2680 2644 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2712 2744 3866 3898 4809
Size: 32 135 32 1140 32 1122 32 911
Cuts at: 4809 4841 6496 6528 6570 6602 1373
Size: 32 1655 32 42 32 2626
Fragments arranged by size:
2626 1655 1140 1122 911 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2301 4753 5127 5199 5445 669
Size: 988 281 363 2452 374 72 246 3079
Fragments arranged by size:
3079 2452 988 374 363 281 246 72
HindIII A'AGCT_T
Cuts at: 748 4719 748
Size: 3971 3884
HpaI GTT'AAC
Cuts at: 5127 5445 5127
Size: 318 7537
KasI G'GCGC_C
Cuts at: 4354 4354
Size: 7855
KpnI G_GTAC'C
Cuts at: 5180 5180
Size: 7855
MfeI C'AATT_G
Cuts at: 5454 5454
Size: 7855
MluI A'CGCG_T
Cuts at: 7731 7731
Size: 7855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4242 4573 6969 7153 1305
Size: 2937 331 2396 184 2007
Fragments arranged by size:
2937 2396 2007 331 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6033
Fragments arranged by size:
6033 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4168 5813 6172 6331 518
Size: 648 3002 1645 359 159 2042
Fragments arranged by size:
3002 2042 1645 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2253 3008 4476 4768 4788 5271 5948 7096
Size: 647 755 1468 292 20 483 677 1148
Cuts at: 7096 7341 1606
Size: 245 2120
Fragments arranged by size:
2120 1468 1148 755 677 647 483 292
245 20
NarI GG'CG_CC
Cuts at: 4355 4355
Size: 7855
NcoI C'CATG_G
Cuts at: 513 1065 2004 2885 4166 4409 4642 513
Size: 552 939 881 1281 243 233 3726
Fragments arranged by size:
3726 1281 939 881 552 243 233
NdeI CA'TA_TG
Cuts at: 387 3031 387
Size: 2644 5211
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7855
NotI GC'GGCC_GC
Cuts at: 5302 5302
Size: 7855
NspI r_CATG'y
Cuts at: 1132 1229 5213 6758 1132
Size: 97 3984 1545 2229
Fragments arranged by size:
3984 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2907 1348
Size: 1559 6296
PciI A'CATG_T
Cuts at: 6754 6754
Size: 7855
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7855
PfoI T'CCnGG_A
Cuts at: 4809 4809
Size: 7855
PmeI GTTT'AAAC
Cuts at: 5159 5159
Size: 7855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5924 5956 7437 7469 5924
Size: 32 1481 32 6310
Fragments arranged by size:
6310 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2955 3572 4929 2955
Size: 617 1357 5881
Fragments arranged by size:
5881 1357 617
PshAI GACnn'nnGTC
Cuts at: 1613 2572 4653 1613
Size: 959 2081 4815
Fragments arranged by size:
4815 2081 959
PsiI TTA'TAA
Cuts at: 4596 5425 4596
Size: 829 7026
PspXI vC'TCGA_Gb
Cuts at: 2598 2598
Size: 7855
PstI C_TGCA'G
Cuts at: 830 3643 4228 4372 5207 830
Size: 2813 585 144 835 3478
Fragments arranged by size:
3478 2813 835 585 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7855
PvuII CAG'CTG
Cuts at: 2253 4476 4768 2253
Size: 2223 292 5340
Fragments arranged by size:
5340 2223 292
SacI G_AGCT'C
Cuts at: 2725 2725
Size: 7855
SalI G'TCGA_C
Cuts at: 1936 5197 1936
Size: 3261 4594
SbfI CC_TGCA'GG
Cuts at: 5207 5207
Size: 7855
ScaI AGT'ACT
Cuts at: 6103 7815 6103
Size: 1712 6143
SexAI A'CCwGG_T
Cuts at: 4851 4851
Size: 7855
SfcI C'TryA_G
Cuts at: 826 1045 2319 2570 2853 2874 3639 3982 4224
Size: 219 1274 251 283 21 765 343 242
Cuts at: 4224 4368 4744 4910 5203 6338 7019 7210 826
Size: 144 376 166 293 1135 681 191 1471
Fragments arranged by size:
1471 1274 1135 765 681 376 343 293
283 251 242 219 191 166 144 21
SfoI GGC'GCC
Cuts at: 4356 4356
Size: 7855
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7855
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7855
SmaI CCC'GGG
Cuts at: 1872 5182 1872
Size: 3310 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2274 2598 2627 2750 3763 4430
Size: 197 926 331 324 29 123 1013 667
Cuts at: 4430 4651 5959 6860 7122 820
Size: 221 1308 901 262 1553
Fragments arranged by size:
1553 1308 1013 926 901 667 331 324
262 221 197 123 29
SnaBI TAC'GTA
Cuts at: 493 5646 493
Size: 5153 2702
SpeI A'CTAG_T
Cuts at: 152 5129 152
Size: 4977 2878
SphI G_CATG'C
Cuts at: 1132 5213 1132
Size: 4081 3774
SspI AAT'ATT
Cuts at: 5 52 4954 5779 5
Size: 47 4902 825 2081
Fragments arranged by size:
4902 2081 825 47
StuI AGG'CCT
Cuts at: 1517 2971 1517
Size: 1454 6401
StyI C'CwwG_G
Cuts at: 513 1065 2004 2437 2885 2939 2958 4166 4409
Size: 552 939 433 448 54 19 1208 243
Cuts at: 4409 4642 4658 5294 513
Size: 233 16 636 3074
Fragments arranged by size:
3074 1208 939 636 552 448 433 243
233 54 19 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4339 5882 5899 6052 6237 6682 325
Size: 1255 2759 1543 17 153 185 445 1498
Fragments arranged by size:
2759 1543 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2089 2418 4801
Size: 274 80 33 51 1443 112 329 2383
Cuts at: 4801 6101 7813 96
Size: 1300 1712 138
Fragments arranged by size:
2383 1712 1443 1300 329 274 138 112
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7815 7847 7815
Size: 32 7823
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7855
XbaI T'CTAG_A
Cuts at: 3934 5191 3934
Size: 1257 6598
XhoI C'TCGA_G
Cuts at: 1943 2598 1943
Size: 655 7200
XmaI C'CCGG_G
Cuts at: 1870 5180 1870
Size: 3310 4545
XmnI GAAnn'nnTTC
Cuts at: 2538 3246 4988 5984 2538
Size: 708 1742 996 4409
Fragments arranged by size:
4409 1742 996 708
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5663 276
Size: 53 83 186 936 4129 2468
Fragments arranged by size:
4129 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI
RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI