Restriction map for
pFN21AB2497
(Circular) (Six-base) MAPSORT of: pFN21AB2497.seq Check: 1771 from: 1 to: 7114
pFN21AB2497 7114 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2886 2985 3345 3627 2886
Size: 99 360 282 6373
Fragments arranged by size:
6373 360 282 99
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4924 278
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4457 1937
Size: 2520 4594
Acc65I G'GTAC_C
Cuts at: 4435 4435
Size: 7114
AclI AA'CG_TT
Cuts at: 1563 5241 5614 1563
Size: 3678 373 3063
Fragments arranged by size:
3678 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2888 5177 6561 6829 1632
Size: 1256 2289 1384 268 1917
Fragments arranged by size:
2289 1917 1384 1256 268
AflII C'TTAA_G
Cuts at: 820 1017 3418 820
Size: 197 2401 4516
Fragments arranged by size:
4516 2401 197
AflIII A'CryG_T
Cuts at: 1203 6013 6990 1203
Size: 4810 977 1327
Fragments arranged by size:
4810 1327 977
AgeI A'CCGG_T
Cuts at: 5945 5945
Size: 7114
AhdI GACnn_n'nnGTC
Cuts at: 3991 5843 3991
Size: 1852 5262
AleI CACnn'nnGTG
Cuts at: 1166 2667 3044 1166
Size: 1501 377 5236
Fragments arranged by size:
5236 1501 377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3966 1184
Size: 2782 4332
AlwNI CAG_nnn'CTG
Cuts at: 2348 2530 3271 4857 6429 2348
Size: 182 741 1586 1572 3033
Fragments arranged by size:
3033 1586 1572 741 182
ApaI G_GGCC'C
Cuts at: 2153 2169 2210 2378 2421 2759 2777 2153
Size: 16 41 168 43 338 18 6490
Fragments arranged by size:
6490 338 168 43 41 18 16
ApaLI G'TGCA_C
Cuts at: 2873 5171 6327 2873
Size: 2298 1156 3660
Fragments arranged by size:
3660 2298 1156
ApoI r'AATT_y
Cuts at: 1493 3330 3760 4423 4649 4801 6910 1493
Size: 1837 430 663 226 152 2109 1697
Fragments arranged by size:
2109 1837 1697 663 430 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5668 160
Size: 872 4636 1606
Fragments arranged by size:
4636 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 2377 2618 2665 2825 4439 1870
Size: 73 225 209 241 47 160 1614 4545
Fragments arranged by size:
4545 1614 241 225 209 160 73 47
BamHI G'GATC_C
Cuts at: 4444 4811 4444
Size: 367 6747
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 2734 2973 3942 4435
Size: 325 217 627 440 507 239 969 493
Cuts at: 4435 5890 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 969 627 507 493 440 325
239 217
BanII G_rGCy'C
Cuts at: 1391 2153 2169 2210 2330 2378 2385 2421 2759
Size: 762 16 41 120 48 7 36 338
Cuts at: 2759 2777 2795 3929 4079 4433 1391
Size: 18 18 1134 150 354 4072
Fragments arranged by size:
4072 1134 762 354 338 150 120 48
41 36 18 18 16 7
BbeI G_GCGC'C
Cuts at: 2231 2738 2231
Size: 507 6607
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3215 3704 928
Size: 971 1316 489 4338
Fragments arranged by size:
4338 1316 971 489
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3145 3179 5305 5339 1903
Size: 34 1208 34 2126 34 3678
Fragments arranged by size:
3678 2126 1208 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2075 4024 5006 6216 2075
Size: 1949 982 1210 2973
Fragments arranged by size:
2973 1949 1210 982
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2173 2195 5725
Size: 107 122 71 631 605 501 22 3530
Cuts at: 5725 136
Size: 1525
Fragments arranged by size:
3530 1525 631 605 501 122 107 71
22
BglII A'GATC_T
Cuts at: 4378 7109 4378
Size: 2731 4383
BlpI GC'TnA_GC
Cuts at: 2706 3744 4531 2706
Size: 1038 787 5289
Fragments arranged by size:
5289 1038 787
Bme1580I G_kGCm'C
Cuts at: 1790 2153 2169 2210 2378 2421 2507 2759 2777
Size: 363 16 41 168 43 86 252 18
Cuts at: 2777 2825 2877 3464 3536 3945 5175 6331 1790
Size: 48 52 587 72 409 1230 1156 2573
Fragments arranged by size:
2573 1230 1156 587 409 363 252 168
86 72 52 48 43 41 18 16
BmgBI CAC'GTC
Cuts at: 1318 2314 1318
Size: 996 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4321 4845 5803
Size: 416 53 83 399 24 2904 524 958
Cuts at: 5803 442
Size: 1753
Fragments arranged by size:
2904 1753 958 524 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2705 1056
Size: 1649 5465
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3948 3948
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 4138 5774 1239
Size: 1130 1769 1636 2579
Fragments arranged by size:
2579 1769 1636 1130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2951 3041 4084 5239 6104 6402 2951
Size: 90 1043 1155 865 298 3663
Fragments arranged by size:
3663 1155 1043 865 298 90
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 3352 4102 5777 882
Size: 617 813 1040 750 1675 2219
Fragments arranged by size:
2219 1675 1040 813 750 617
BsaAI yAC'GTr
Cuts at: 493 1204 4905 493
Size: 711 3701 2702
Fragments arranged by size:
3701 2702 711
BsaBI GATnn'nnATC
Cuts at: 7108 7108
Size: 7114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2228 2553 2735 4921
Size: 53 83 186 936 695 325 182 2186
Cuts at: 4921 5303 5938 275
Size: 382 635 1451
Fragments arranged by size:
2186 1451 936 695 635 382 325 186
182 83 53
BsaWI w'CCGG_w
Cuts at: 2330 3447 5547 5945 6219 6366 6777 2330
Size: 1117 2100 398 274 147 411 2667
Fragments arranged by size:
2667 2100 1117 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3590 3620 1178
Size: 30 165 30 2187 30 4672
Fragments arranged by size:
4672 2187 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2375 2384 2756 2759 3128 3649 4008
Size: 1012 177 9 372 3 369 521 359
Cuts at: 4008 1186
Size: 4292
Fragments arranged by size:
4292 1012 521 372 369 359 177 9
3
BseYI C'CCAG_C
Cuts at: 2610 2642 2788 3773 4181 6317 6813 2610
Size: 32 146 985 408 2136 496 2911
Fragments arranged by size:
2911 2136 985 496 408 146 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6541
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 2715 2763 4564 5325 6353
Size: 275 186 82 632 48 1801 761 1028
Cuts at: 6353 1540
Size: 2301
Fragments arranged by size:
2301 1801 1028 761 632 275 186 82
48
BsiHKAI G_wGCw'C
Cuts at: 2877 3232 3929 4079 4229 5175 5260 6331 2877
Size: 355 697 150 150 946 85 1071 3660
Fragments arranged by size:
3660 1071 946 697 355 150 150 85
BsmI GAATG_Cn'
Cuts at: 4625 4718 4625
Size: 93 7021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2169 2210 2330 2378 2385 2421
Size: 399 363 16 41 120 48 7 36
Cuts at: 2421 2507 2759 2777 2795 2825 2877 3232 3464
Size: 86 252 18 18 30 52 355 232
Cuts at: 3464 3536 3929 3945 4079 4229 4433 5175 5260
Size: 72 393 16 134 150 204 742 85
Cuts at: 5260 6331 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 363 355 252
232 204 150 134 120 86 85 72
52 48 41 36 30 18 18 16
16 7
BspEI T'CCGG_A
Cuts at: 6777 6777
Size: 7114
BspHI T'CATG_A
Cuts at: 5003 6733 5003
Size: 1730 5384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2538 2886 2985 3345 3627 4469 844
Size: 1694 348 99 360 282 842 3489
Fragments arranged by size:
3489 1694 842 360 348 282 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2400 2603 5001 1394
Size: 429 577 203 2398 3507
Fragments arranged by size:
3507 2398 577 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 3866 5609 5783 65
Size: 1183 2618 1743 174 1396
Fragments arranged by size:
2618 1743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2710 3350 5758 5945 1700
Size: 110 297 603 640 2408 187 2869
Fragments arranged by size:
2869 2408 640 603 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 3048 96
Size: 2952 4162
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7114
BssSI C'ACGA_G
Cuts at: 3490 5174 6186 3490
Size: 1684 1012 4418
Fragments arranged by size:
4418 1684 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7114
BstBI TT'CG_AA
Cuts at: 3165 5918 3165
Size: 2753 4361
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7114
BstXI CCAn_nnnn'nTGG
Cuts at: 2586 2586
Size: 7114
BstYI r'GATC_y
Cuts at: 1972 3733 4124 4378 4444 4811 5196 5213 6665
Size: 1761 391 254 66 367 385 17 1452
Cuts at: 6665 6751 6763 7109 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1761 1452 391 385 367 346 254
86 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 2739 4167 2739
Size: 1428 5686
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2322 2412 2448 2490 2779
Size: 552 939 66 252 90 36 42 289
Cuts at: 2779 4357 513
Size: 1578 3270
Fragments arranged by size:
3270 1578 939 552 289 252 90 66
42 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6375
Fragments arranged by size:
6375 683 56
BtsI GCAGTG_nn'
Cuts at: 2421 2840 3784 4630 5424 5444 2421
Size: 419 944 846 794 20 4091
Fragments arranged by size:
4091 944 846 794 419 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7079
DraI TTT'AAA
Cuts at: 4418 4418
Size: 7114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6121 809
Size: 507 4805 1802
Fragments arranged by size:
4805 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2113 2565
Size: 54 1425 50 275 18 250 33 452
Cuts at: 2565 2712 2760 4002 4056 4140 4561 5450 7079
Size: 147 48 1242 54 84 421 889 1629
Cuts at: 7079 8
Size: 43
Fragments arranged by size:
1629 1425 1242 889 452 421 275 250
147 84 54 54 50 48 43 33
18
EagI C'GGCC_G
Cuts at: 1537 1812 2080 2712 2760 4561 1537
Size: 275 268 632 48 1801 4090
Fragments arranged by size:
4090 1801 632 275 268 48
EarI CTCTTCn'nnn_
Cuts at: 1353 3680 3839 5044 1353
Size: 2327 159 1205 3423
Fragments arranged by size:
3423 2327 1205 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2464 2765 3512 5701 6075 6221
Size: 1064 138 875 301 747 2189 374 146
Cuts at: 6221 387
Size: 1280
Fragments arranged by size:
2189 1280 1064 875 747 374 301 146
138
EcoICRI GAG'CTC
Cuts at: 3927 4077 3927
Size: 150 6964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2888 4138 5177 5774 6561 6829
Size: 393 737 519 1250 1039 597 787 268
Cuts at: 6829 1239
Size: 1524
Fragments arranged by size:
1524 1250 1039 787 737 597 519 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3468 3558 3468
Size: 90 7024
EcoO109I rG'GnC_Cy
Cuts at: 2096 2119 2149 2150 2206 2207 2345 2374 2417
Size: 23 30 1 56 1 138 29 43
Cuts at: 2417 2432 2527 2755 2774 2910 2096
Size: 15 95 228 19 136 6300
Fragments arranged by size:
6300 228 138 136 95 56 43 30
29 23 19 15 1 1
EcoRI G'AATT_C
Cuts at: 4423 4801 4423
Size: 378 6736
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 2231 2738 3147 6261 1678
Size: 553 507 409 3114 2531
Fragments arranged by size:
3114 2531 553 507 409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 3812 3814 3844
Size: 32 135 32 652 32 1556 2 30
Cuts at: 3844 3846 3977 4009 4082 4114 5755 5787 5829
Size: 2 131 32 73 32 1641 32 42
Cuts at: 5829 5861 1373
Size: 32 2626
Fragments arranged by size:
2626 1641 1556 652 135 131 73 42
32 32 32 32 32 32 32 30
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4458 4704 669
Size: 988 281 2520 246 3079
Fragments arranged by size:
3079 2520 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7114
HpaI GTT'AAC
Cuts at: 4704 4704
Size: 7114
KasI G'GCGC_C
Cuts at: 2227 2734 2227
Size: 507 6607
KpnI G_GTAC'C
Cuts at: 4439 4439
Size: 7114
MfeI C'AATT_G
Cuts at: 4713 4713
Size: 7114
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6228 6412 1305
Size: 4923 184 2007
Fragments arranged by size:
4923 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2115 2567 4004 4142 10
Size: 54 1425 343 283 452 1437 138 2982
Fragments arranged by size:
2982 1437 1425 452 343 283 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2667 3044 3097 3404 3441 5072 5431
Size: 648 1501 377 53 307 37 1631 359
Cuts at: 5431 5590 518
Size: 159 2042
Fragments arranged by size:
2042 1631 1501 648 377 359 307 159
53 37
MspA1I CmG'CkG
Cuts at: 1606 2072 2324 2414 2439 2450 2492 2781 2788
Size: 466 252 90 25 11 42 289 7
Cuts at: 2788 2866 4530 5207 6355 6600 1606
Size: 78 1664 677 1148 245 2120
Fragments arranged by size:
2120 1664 1148 677 466 289 252 245
90 78 42 25 11 7
NaeI GCC'GGC
Cuts at: 2109 2712 2109
Size: 603 6511
NarI GG'CG_CC
Cuts at: 2228 2735 2228
Size: 507 6607
NcoI C'CATG_G
Cuts at: 513 1065 2004 4357 513
Size: 552 939 2353 3270
Fragments arranged by size:
3270 2353 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7114
NgoMIV G'CCGG_C
Cuts at: 2107 2710 2107
Size: 603 6511
NheI G'CTAG_C
Cuts at: 1052 2701 1052
Size: 1649 5465
NotI GC'GGCC_GC
Cuts at: 4561 4561
Size: 7114
NspI r_CATG'y
Cuts at: 1132 1229 2657 3409 4472 6017 1132
Size: 97 1428 752 1063 1545 2229
Fragments arranged by size:
2229 1545 1428 1063 752 97
PasI CC'CwG_GG
Cuts at: 1348 2770 2926 1348
Size: 1422 156 5536
Fragments arranged by size:
5536 1422 156
PciI A'CATG_T
Cuts at: 6013 6013
Size: 7114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7114
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5183 5215 6696 6728 5183
Size: 32 1481 32 5569
Fragments arranged by size:
5569 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2527 2119
Size: 408 6706
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7114
PsiI TTA'TAA
Cuts at: 4684 4684
Size: 7114
PspOMI G'GGCC_C
Cuts at: 2149 2165 2206 2374 2417 2755 2773 2149
Size: 16 41 168 43 338 18 6490
Fragments arranged by size:
6490 338 168 43 41 18 16
PstI C_TGCA'G
Cuts at: 830 2982 3299 3624 4466 830
Size: 2152 317 325 842 3478
Fragments arranged by size:
3478 2152 842 325 317
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7114
PvuII CAG'CTG
Cuts at: 2866 2866
Size: 7114
RsrII CG'GwC_CG
Cuts at: 2783 2783
Size: 7114
SacI G_AGCT'C
Cuts at: 3929 4079 3929
Size: 150 6964
SacII CC_GC'GG
Cuts at: 2073 2325 2415 2451 2493 2782 2073
Size: 252 90 36 42 289 6405
Fragments arranged by size:
6405 289 252 90 42 36
SalI G'TCGA_C
Cuts at: 1936 4456 1936
Size: 2520 4594
SbfI CC_TGCA'GG
Cuts at: 3624 4466 3624
Size: 842 6272
ScaI AGT'ACT
Cuts at: 3856 5362 7074 3856
Size: 1506 1712 3896
Fragments arranged by size:
3896 1712 1506
SfcI C'TryA_G
Cuts at: 826 1045 2042 2978 3295 3620 4462 5597 6278
Size: 219 997 936 317 325 842 1135 681
Cuts at: 6278 6469 826
Size: 191 1471
Fragments arranged by size:
1471 1135 997 936 842 681 325 317
219 191
SfoI GGC'GCC
Cuts at: 2229 2736 2229
Size: 507 6607
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 3350 1700
Size: 1650 5464
SmaI CCC'GGG
Cuts at: 1872 2170 2379 2620 2667 4441 1872
Size: 298 209 241 47 1774 4545
Fragments arranged by size:
4545 1774 298 241 209 47
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2966 3056 3418 4099 5218 6119
Size: 197 926 1023 90 362 681 1119 901
Cuts at: 6119 6381 820
Size: 262 1553
Fragments arranged by size:
1553 1119 1023 926 901 681 362 262
197 90
SnaBI TAC'GTA
Cuts at: 493 4905 493
Size: 4412 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 2657 4472 1132
Size: 1525 1815 3774
Fragments arranged by size:
3774 1815 1525
SrfI GCCC'GGGC
Cuts at: 2170 2170
Size: 7114
SspI AAT'ATT
Cuts at: 5 52 5038 5
Size: 47 4986 2081
Fragments arranged by size:
4986 2081 47
StuI AGG'CCT
Cuts at: 1517 3178 1517
Size: 1661 5453
StyI C'CwwG_G
Cuts at: 513 1065 2004 4357 4553 513
Size: 552 939 2353 196 3074
Fragments arranged by size:
3074 2353 939 552 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2294 2918 3787 4195 5141 5158
Size: 1255 672 42 624 869 408 946 17
Cuts at: 5158 5311 5496 5941 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 946 869 672 624 445 408
185 153 42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 3048 3854
Size: 274 80 33 51 1443 837 234 806
Cuts at: 3854 5360 7072 96
Size: 1506 1712 138
Fragments arranged by size:
1712 1506 1443 837 806 274 234 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7074 7106 7074
Size: 32 7082
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7114
XbaI T'CTAG_A
Cuts at: 4450 4450
Size: 7114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2583 2583
Size: 7114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7114
XmaI C'CCGG_G
Cuts at: 1870 2168 2377 2618 2665 4439 1870
Size: 298 209 241 47 1774 4545
Fragments arranged by size:
4545 1774 298 241 209 47
XmnI GAAnn'nnTTC
Cuts at: 3092 3485 5243 3092
Size: 393 1758 4963
Fragments arranged by size:
4963 1758 393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4922 276
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI SanDI
SapI SexAI SfiI SwaI