(Circular) (Six-base) MAPSORT of: pFN21AB2389.seq Check: 2466 from: 1 to: 7879 pFN21AB2389 7879 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2979 3115 3427 5042 2979 Size: 136 312 1615 5816 Fragments arranged by size: 5816 1615 312 136 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2493 3396 5689 278 Size: 53 83 186 936 957 903 2293 2468 Fragments arranged by size: 2468 2293 957 936 903 186 83 53 AccI GT'mk_AC Cuts at: 1937 4005 5222 1937 Size: 2068 1217 4594 Fragments arranged by size: 4594 2068 1217 Acc65I G'GTAC_C Cuts at: 5200 5200 Size: 7879 AclI AA'CG_TT Cuts at: 1563 6006 6379 1563 Size: 4443 373 3063 Fragments arranged by size: 4443 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3052 3206 3980 4084 4111 4346 4730 5157 Size: 1420 154 774 104 27 235 384 427 Cuts at: 5157 5942 7326 7594 1632 Size: 785 1384 268 1917 Fragments arranged by size: 1917 1420 1384 785 774 427 384 268 235 154 104 27 AfeI AGC'GCT Cuts at: 2432 5102 2432 Size: 2670 5209 AflII C'TTAA_G Cuts at: 820 1017 2152 820 Size: 197 1135 6547 Fragments arranged by size: 6547 1135 197 AflIII A'CryG_T Cuts at: 1203 2125 2443 2628 4047 6778 7755 1203 Size: 922 318 185 1419 2731 977 1327 Fragments arranged by size: 2731 1419 1327 977 922 318 185 AgeI A'CCGG_T Cuts at: 6710 6710 Size: 7879 AhdI GACnn_n'nnGTC Cuts at: 6608 6608 Size: 7879 AleI CACnn'nnGTG Cuts at: 1166 4946 1166 Size: 3780 4099 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4066 1184 Size: 2882 4997 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 2276 Size: 32 7847 AlwNI CAG_nnn'CTG Cuts at: 2389 2869 3932 4915 5622 7194 2389 Size: 480 1063 983 707 1572 3074 Fragments arranged by size: 3074 1572 1063 983 707 480 ApaI G_GGCC'C Cuts at: 2702 3343 2702 Size: 641 7238 ApaLI G'TGCA_C Cuts at: 3668 5936 7092 3668 Size: 2268 1156 4455 Fragments arranged by size: 4455 2268 1156 ApoI r'AATT_y Cuts at: 1493 3965 5188 5414 5566 7675 1493 Size: 2472 1223 226 152 2109 1697 Fragments arranged by size: 2472 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 6433 160 Size: 872 5401 1606 Fragments arranged by size: 5401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 AvaI C'yCGr_G Cuts at: 1870 1943 2701 5204 1870 Size: 73 758 2503 4545 Fragments arranged by size: 4545 2503 758 73 AvrII C'CTAG_G Cuts at: 2011 2011 Size: 7879 BamHI G'GATC_C Cuts at: 2691 5209 5576 2691 Size: 2518 367 4994 Fragments arranged by size: 4994 2518 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3722 4161 5145 5200 6655 Size: 325 217 627 1935 439 984 55 1455 Cuts at: 6655 618 Size: 1842 Fragments arranged by size: 1935 1842 1455 984 627 439 325 217 55 BanII G_rGCy'C Cuts at: 1391 2702 3343 3885 4209 4449 5198 1391 Size: 1311 641 542 324 240 749 4072 Fragments arranged by size: 4072 1311 749 641 542 324 240 BbeI G_GCGC'C Cuts at: 5149 5149 Size: 7879 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 2475 3582 4404 928 Size: 971 393 183 1107 822 4403 Fragments arranged by size: 4403 1107 971 822 393 183 BbvCI CC'TCA_GC Cuts at: 5121 5121 Size: 7879 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3163 3197 6070 6104 1903 Size: 34 1226 34 2873 34 3678 Fragments arranged by size: 3678 2873 1226 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 5771 6981 3518 Size: 2253 1210 4416 Fragments arranged by size: 4416 2253 1210 BclI T'GATC_A Cuts at: 1548 2214 1548 Size: 666 7213 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2732 3650 6490 Size: 107 122 71 631 605 1060 918 2840 Cuts at: 6490 136 Size: 1525 Fragments arranged by size: 2840 1525 1060 918 631 605 122 107 71 BglII A'GATC_T Cuts at: 2160 4380 7874 2160 Size: 2220 3494 2165 Fragments arranged by size: 3494 2220 2165 BlpI GC'TnA_GC Cuts at: 5296 5296 Size: 7879 Bme1580I G_kGCm'C Cuts at: 1790 2636 2702 3343 3672 4347 5092 5940 7096 Size: 846 66 641 329 675 745 848 1156 Cuts at: 7096 1790 Size: 2573 Fragments arranged by size: 2573 1156 848 846 745 675 641 329 66 BmgBI CAC'GTC Cuts at: 1318 2473 4522 1318 Size: 1155 2049 4675 Fragments arranged by size: 4675 2049 1155 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 2539 2925 Size: 416 53 83 399 24 711 411 386 Cuts at: 2925 5610 6568 442 Size: 2685 958 1753 Fragments arranged by size: 2685 1753 958 711 416 411 399 386 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7879 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 4261 4426 2182 Size: 2079 165 5635 Fragments arranged by size: 5635 2079 165 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4055 4223 4462 4664 5066 6539 1239 Size: 2816 168 239 202 402 1473 2579 Fragments arranged by size: 2816 2579 1473 402 239 202 168 Bpu10I CC'TnA_GC Cuts at: 2612 5121 2612 Size: 2509 5370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3962 4003 6004 6869 7167 3962 Size: 41 2001 865 298 4674 Fragments arranged by size: 4674 2001 865 298 41 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2930 3136 4232 6542 882 Size: 617 1431 206 1096 2310 2219 Fragments arranged by size: 2310 2219 1431 1096 617 206 BsaAI yAC'GTr Cuts at: 493 1204 4819 5670 493 Size: 711 3615 851 2702 Fragments arranged by size: 3615 2702 851 711 BsaBI GATnn'nnATC Cuts at: 2219 4025 4196 7873 2219 Size: 1806 171 3677 2225 Fragments arranged by size: 3677 2225 1806 171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2490 3393 5146 5686 Size: 53 83 186 936 957 903 1753 540 Cuts at: 5686 6068 6703 275 Size: 382 635 1451 Fragments arranged by size: 1753 1451 957 936 903 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6312 6710 6984 7131 7542 6312 Size: 398 274 147 411 6649 Fragments arranged by size: 6649 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4145 4175 4196 4226 1178 Size: 30 165 30 2742 30 21 30 4831 Fragments arranged by size: 4831 2742 165 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2155 3005 4085 4550 4646 4820 1186 Size: 969 850 1080 465 96 174 4245 Fragments arranged by size: 4245 1080 969 850 465 174 96 BseYI C'CCAG_C Cuts at: 2184 7082 7578 2184 Size: 4898 496 2485 Fragments arranged by size: 4898 2485 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2948 3718 3803 4615 4949 1163 Size: 573 1212 770 85 812 334 4093 Fragments arranged by size: 4093 1212 812 770 573 334 85 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5329 6090 7118 1540 Size: 275 186 3328 761 1028 2301 Fragments arranged by size: 3328 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3009 3179 3672 3885 4209 5940 6025 7096 3009 Size: 170 493 213 324 1731 85 1071 3792 Fragments arranged by size: 3792 1731 1071 493 324 213 170 85 BsmI GAATG_Cn' Cuts at: 5390 5483 5390 Size: 93 7786 BsmBI CGTCTCn'nnnn_ Cuts at: 4480 4480 Size: 7879 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2636 2702 3009 3179 3343 3672 3885 Size: 399 846 66 307 170 164 329 213 Cuts at: 3885 4209 4347 4449 5092 5198 5940 6025 7096 Size: 324 138 102 643 106 742 85 1071 Cuts at: 7096 1391 Size: 2174 Fragments arranged by size: 2174 1071 846 742 643 399 329 324 307 213 170 164 138 106 102 85 66 BspEI T'CCGG_A Cuts at: 7542 7542 Size: 7879 BspHI T'CATG_A Cuts at: 5768 7498 5768 Size: 1730 6149 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2580 2979 3115 3427 3494 4085 5042 5234 Size: 1736 399 136 312 67 591 957 192 Cuts at: 5234 844 Size: 3489 Fragments arranged by size: 3489 1736 957 591 399 312 192 136 67 BsrBI CCG'CTC Cuts at: 1394 1823 5766 1394 Size: 429 3943 3507 Fragments arranged by size: 3943 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2562 3219 5115 6374 6548 65 Size: 1183 1314 657 1896 1259 174 1396 Fragments arranged by size: 1896 1396 1314 1259 1183 657 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3042 6523 6710 1700 Size: 110 1232 3481 187 2869 Fragments arranged by size: 3481 2869 1232 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7879 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7879 BssSI C'ACGA_G Cuts at: 5093 5939 6951 5093 Size: 846 1012 6021 Fragments arranged by size: 6021 1012 846 BstAPI GCAn_nnn'nTGC Cuts at: 794 2971 3004 4585 794 Size: 2177 33 1581 4088 Fragments arranged by size: 4088 2177 1581 33 BstBI TT'CG_AA Cuts at: 6683 6683 Size: 7879 BstEII G'GTnAC_C Cuts at: 1184 2519 1184 Size: 1335 6544 BstXI CCAn_nnnn'nTGG Cuts at: 2947 3226 3811 2947 Size: 279 585 7015 Fragments arranged by size: 7015 585 279 BstYI r'GATC_y Cuts at: 1972 2160 2679 2691 3429 4020 4183 4380 5209 Size: 188 519 12 738 591 163 197 829 Cuts at: 5209 5576 5961 5978 7430 7516 7528 7874 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 829 738 591 519 385 367 346 197 188 163 86 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2088 3164 3767 4319 4533 4602 4751 2088 Size: 1076 603 552 214 69 149 5216 Fragments arranged by size: 5216 1076 603 552 214 149 69 BtgI C'CryG_G Cuts at: 513 1065 2004 2665 2814 3225 3648 513 Size: 552 939 661 149 411 423 4744 Fragments arranged by size: 4744 939 661 552 423 411 149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7140 Fragments arranged by size: 7140 683 56 BtsI GCAGTG_nn' Cuts at: 3750 5395 6189 6209 3750 Size: 1645 794 20 5420 Fragments arranged by size: 5420 1645 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3621 3656 4357 4392 529 Size: 35 3057 35 701 35 4016 Fragments arranged by size: 4016 3057 701 35 35 35 DraI TTT'AAA Cuts at: 5183 5183 Size: 7879 DraIII CAC_nnn'GTG Cuts at: 2251 2251 Size: 7879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6886 809 Size: 507 5570 1802 Fragments arranged by size: 5570 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2652 2724 Size: 54 1425 50 275 18 237 585 72 Cuts at: 2724 2811 2919 3228 4974 5326 6215 7844 8 Size: 87 108 309 1746 352 889 1629 43 Fragments arranged by size: 1746 1629 1425 889 585 352 309 275 237 108 87 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5326 1537 Size: 275 3514 4090 Fragments arranged by size: 4090 3514 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 3350 3942 3993 4103 4322 4751 5809 Size: 992 1005 592 51 110 219 429 1058 Cuts at: 5809 1353 Size: 3423 Fragments arranged by size: 3423 1058 1005 992 592 429 219 110 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3158 6466 6840 6986 387 Size: 1064 138 1569 3308 374 146 1280 Fragments arranged by size: 3308 1569 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3883 4207 3883 Size: 324 7555 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3052 3206 3980 4055 4084 4111 4223 Size: 393 1420 154 774 75 29 27 112 Cuts at: 4223 4346 4462 4664 4730 5066 5157 5942 6539 Size: 123 116 202 66 336 91 785 597 Cuts at: 6539 7326 7594 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1420 787 785 774 597 393 336 268 202 154 123 116 112 91 75 66 29 27 EcoNI CCTnn'n_nnAGG Cuts at: 3110 4803 4919 3110 Size: 1693 116 6070 Fragments arranged by size: 6070 1693 116 EcoO109I rG'GnC_Cy Cuts at: 2015 2530 3195 3339 3340 3532 3788 4912 2015 Size: 515 665 144 1 192 256 1124 4982 Fragments arranged by size: 4982 1124 665 515 256 192 144 1 EcoRI G'AATT_C Cuts at: 5188 5566 5188 Size: 378 7501 EcoRV GAT'ATC Cuts at: 3208 3208 Size: 7879 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3316 4351 3316 Size: 1035 6844 FspI TGC'GCA Cuts at: 6385 6385 Size: 7879 HaeII r_GCGC'y Cuts at: 1678 2434 2870 4618 5104 5149 7026 1678 Size: 756 436 1748 486 45 1877 2531 Fragments arranged by size: 2531 1877 1748 756 486 436 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2124 2156 2364 2396 2671 Size: 32 135 32 552 32 208 32 275 Cuts at: 2671 2703 4208 4240 4489 4521 6520 6552 6594 Size: 32 1505 32 249 32 1999 32 42 Cuts at: 6594 6626 1373 Size: 32 2626 Fragments arranged by size: 2626 1999 1505 552 275 249 208 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2455 5223 5469 669 Size: 988 281 517 2768 246 3079 Fragments arranged by size: 3079 2768 988 517 281 246 HindIII A'AGCT_T Cuts at: 748 2364 4388 748 Size: 1616 2024 4239 Fragments arranged by size: 4239 2024 1616 HpaI GTT'AAC Cuts at: 5469 5469 Size: 7879 KasI G'GCGC_C Cuts at: 5145 5145 Size: 7879 KpnI G_GTAC'C Cuts at: 5204 5204 Size: 7879 MfeI C'AATT_G Cuts at: 5478 5478 Size: 7879 MluI A'CGCG_T Cuts at: 2628 4047 7755 2628 Size: 1419 3708 2752 Fragments arranged by size: 3708 2752 1419 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 2286 2669 6993 7177 1305 Size: 805 176 383 4324 184 2007 Fragments arranged by size: 4324 2007 805 383 184 176 MscI TGG'CCA Cuts at: 10 64 1489 1832 2654 2726 2813 2921 4976 Size: 54 1425 343 822 72 87 108 2055 Cuts at: 4976 10 Size: 2913 Fragments arranged by size: 2913 2055 1425 822 343 108 87 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 2945 3224 3809 4946 4976 5837 6196 Size: 648 1779 279 585 1137 30 861 359 Cuts at: 6196 6355 518 Size: 159 2042 Fragments arranged by size: 2042 1779 1137 861 648 585 359 279 159 30 MspA1I CmG'CkG Cuts at: 1606 2077 2667 3404 4482 4627 4883 5295 5972 Size: 471 590 737 1078 145 256 412 677 Cuts at: 5972 7120 7365 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1078 737 677 590 471 412 256 245 145 NarI GG'CG_CC Cuts at: 5146 5146 Size: 7879 NcoI C'CATG_G Cuts at: 513 1065 2004 3225 513 Size: 552 939 1221 5167 Fragments arranged by size: 5167 1221 939 552 NdeI CA'TA_TG Cuts at: 387 3258 387 Size: 2871 5008 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7879 NotI GC'GGCC_GC Cuts at: 5326 5326 Size: 7879 NspI r_CATG'y Cuts at: 1132 1229 2447 2743 5237 6782 1132 Size: 97 1218 296 2494 1545 2229 Fragments arranged by size: 2494 2229 1545 1218 296 97 PasI CC'CwG_GG Cuts at: 1348 4424 1348 Size: 3076 4803 PciI A'CATG_T Cuts at: 2443 6778 2443 Size: 4335 3544 PflMI CCAn_nnn'nTGG Cuts at: 1108 4117 4201 1108 Size: 3009 84 4786 Fragments arranged by size: 4786 3009 84 PfoI T'CCnGG_A Cuts at: 2484 3453 4200 4854 2484 Size: 969 747 654 5509 Fragments arranged by size: 5509 969 747 654 PmeI GTTT'AAAC Cuts at: 5183 5183 Size: 7879 PmlI CAC'GTG Cuts at: 4819 4819 Size: 7879 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2063 2095 5948 5980 7461 7493 2063 Size: 32 3853 32 1481 32 2449 Fragments arranged by size: 3853 2449 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2015 2530 3788 4912 2015 Size: 515 1258 1124 4982 Fragments arranged by size: 4982 1258 1124 515 PshAI GACnn'nnGTC Cuts at: 1613 4011 1613 Size: 2398 5481 PsiI TTA'TAA Cuts at: 5449 5449 Size: 7879 PspOMI G'GGCC_C Cuts at: 2698 3339 2698 Size: 641 7238 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3499 3531 3499 Size: 32 7847 PstI C_TGCA'G Cuts at: 830 2176 2241 4775 5231 830 Size: 1346 65 2534 456 3478 Fragments arranged by size: 3478 2534 1346 456 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7879 PvuII CAG'CTG Cuts at: 2077 3404 4627 4883 2077 Size: 1327 1223 256 5073 Fragments arranged by size: 5073 1327 1223 256 SacI G_AGCT'C Cuts at: 3885 4209 3885 Size: 324 7555 SacII CC_GC'GG Cuts at: 2668 2668 Size: 7879 SalI G'TCGA_C Cuts at: 1936 5221 1936 Size: 3285 4594 SanDI GG'GwC_CC Cuts at: 3788 3788 Size: 7879 SapI GCTCTTCn'nnn_ Cuts at: 3942 4322 3942 Size: 380 7499 SbfI CC_TGCA'GG Cuts at: 5231 5231 Size: 7879 ScaI AGT'ACT Cuts at: 6127 7839 6127 Size: 1712 6167 SfcI C'TryA_G Cuts at: 826 1045 2172 2237 4771 5227 6362 7043 7234 Size: 219 1127 65 2534 456 1135 681 191 Cuts at: 7234 826 Size: 1471 Fragments arranged by size: 2534 1471 1135 1127 681 456 219 191 65 SfiI GGCCn_nnn'nGGCC Cuts at: 3650 3650 Size: 7879 SfoI GGC'GCC Cuts at: 5147 5147 Size: 7879 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7879 SmaI CCC'GGG Cuts at: 1872 2703 5206 1872 Size: 831 2503 4545 Fragments arranged by size: 4545 2503 831 SmlI C'TyrA_G Cuts at: 820 1017 1943 2152 3941 3982 5983 6884 7146 Size: 197 926 209 1789 41 2001 901 262 Cuts at: 7146 820 Size: 1553 Fragments arranged by size: 2001 1789 1553 926 901 262 209 197 41 SnaBI TAC'GTA Cuts at: 493 5670 493 Size: 5177 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7879 SphI G_CATG'C Cuts at: 1132 5237 1132 Size: 4105 3774 SspI AAT'ATT Cuts at: 5 52 5803 5 Size: 47 5751 2081 Fragments arranged by size: 5751 2081 47 StuI AGG'CCT Cuts at: 1517 2150 1517 Size: 633 7246 StyI C'CwwG_G Cuts at: 513 1065 2004 2011 2229 3111 3198 3225 3858 Size: 552 939 7 218 882 87 27 633 Cuts at: 3858 3916 4149 4589 4889 5019 5318 513 Size: 58 233 440 300 130 299 3074 Fragments arranged by size: 3074 939 882 633 552 440 300 299 233 218 130 87 58 27 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2858 3740 3871 4437 4958 5906 5923 Size: 1255 1278 882 131 566 521 948 17 Cuts at: 5923 6076 6261 6706 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1278 1255 948 882 566 521 445 185 153 131 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6125 7837 96 Size: 274 80 33 51 1443 4148 1712 138 Fragments arranged by size: 4148 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 2701 2733 2833 2865 4145 4177 4196 Size: 32 189 32 100 32 1280 32 19 Cuts at: 4196 4228 7839 7871 2480 Size: 32 3611 32 2488 Fragments arranged by size: 3611 2488 1280 189 100 32 32 32 32 32 32 19 Tth111I GACn'n_nGTC Cuts at: 1316 2257 4862 1316 Size: 941 2605 4333 Fragments arranged by size: 4333 2605 941 XbaI T'CTAG_A Cuts at: 4036 5215 4036 Size: 1179 6700 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2647 4639 2647 Size: 1992 5887 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7879 XmaI C'CCGG_G Cuts at: 1870 2701 5204 1870 Size: 831 2503 4545 Fragments arranged by size: 4545 2503 831 XmnI GAAnn'nnTTC Cuts at: 4331 4683 6008 4331 Size: 352 1325 6202 Fragments arranged by size: 6202 1325 352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2491 3394 5687 276 Size: 53 83 186 936 957 903 2293 2468 Fragments arranged by size: 2468 2293 957 936 903 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SexAI SrfI SwaI