(Circular) (Six-base) MAPSORT of: pFN21AB2048.seq Check: 5298 from: 1 to: 7756 pFN21AB2048 7756 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5566 278 Size: 53 83 186 936 4030 2468 Fragments arranged by size: 4030 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4827 5099 1937 Size: 2890 272 4594 Fragments arranged by size: 4594 2890 272 Acc65I G'GTAC_C Cuts at: 4836 5077 4836 Size: 241 7515 AclI AA'CG_TT Cuts at: 1563 5883 6256 1563 Size: 4320 373 3063 Fragments arranged by size: 4320 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 4067 4187 4985 5819 7203 7471 1632 Size: 404 2031 120 798 834 1384 268 1917 Fragments arranged by size: 2031 1917 1384 834 798 404 268 120 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7559 AflIII A'CryG_T Cuts at: 1203 2945 6655 7632 1203 Size: 1742 3710 977 1327 Fragments arranged by size: 3710 1742 1327 977 AgeI A'CCGG_T Cuts at: 6587 6587 Size: 7756 AhdI GACnn_n'nnGTC Cuts at: 6485 6485 Size: 7756 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7756 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7756 AlwNI CAG_nnn'CTG Cuts at: 3241 3508 3566 5499 7071 3241 Size: 267 58 1933 1572 3926 Fragments arranged by size: 3926 1933 1572 267 58 ApaLI G'TGCA_C Cuts at: 5813 6969 5813 Size: 1156 6600 ApoI r'AATT_y Cuts at: 1493 2499 2828 5065 5291 5443 7552 1493 Size: 1006 329 2237 226 152 2109 1697 Fragments arranged by size: 2237 2109 1697 1006 329 226 152 AseI AT'TA_AT Cuts at: 160 1032 6310 160 Size: 872 5278 1606 Fragments arranged by size: 5278 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 AvaI C'yCGr_G Cuts at: 1870 1943 3457 3816 4690 5081 1870 Size: 73 1514 359 874 391 4545 Fragments arranged by size: 4545 1514 874 391 359 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3508 3541 3508 Size: 33 7723 BamHI G'GATC_C Cuts at: 4215 5086 5453 4215 Size: 871 367 6518 Fragments arranged by size: 6518 871 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2802 3473 3542 4836 5077 Size: 325 217 627 1015 671 69 1294 241 Cuts at: 5077 6532 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1294 1015 671 627 325 241 217 69 BanII G_rGCy'C Cuts at: 1391 3278 3464 5075 1391 Size: 1887 186 1611 4072 Fragments arranged by size: 4072 1887 1611 186 BbeI G_GCGC'C Cuts at: 3546 3546 Size: 7756 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2786 928 Size: 971 887 5898 Fragments arranged by size: 5898 971 887 BbvCI CC'TCA_GC Cuts at: 3809 3809 Size: 7756 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2311 2345 4860 4894 5947 5981 1903 Size: 34 374 34 2515 34 1053 34 3678 Fragments arranged by size: 3678 2515 1053 374 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5648 6858 5648 Size: 1210 6546 BclI T'GATC_A Cuts at: 1548 1548 Size: 7756 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6367 136 Size: 107 122 71 631 605 4695 1525 Fragments arranged by size: 4695 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4482 7751 4482 Size: 3269 4487 BlpI GC'TnA_GC Cuts at: 2687 3277 4157 5173 2687 Size: 590 880 1016 5270 Fragments arranged by size: 5270 1016 880 590 Bme1580I G_kGCm'C Cuts at: 1790 2805 3476 5817 6973 1790 Size: 1015 671 2341 1156 2573 Fragments arranged by size: 2573 2341 1156 1015 671 BmgBI CAC'GTC Cuts at: 1318 4243 1318 Size: 2925 4831 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4502 4901 Size: 416 53 83 399 24 2009 1076 399 Cuts at: 4901 5487 6445 442 Size: 586 958 1753 Fragments arranged by size: 2009 1753 1076 958 586 416 399 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2444 1056 Size: 1388 6368 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3292 3292 Size: 7756 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2127 2135 2320 3505 3848 3972 4070 Size: 876 12 8 185 1185 343 124 98 Cuts at: 4070 6416 1239 Size: 2346 2579 Fragments arranged by size: 2579 2346 1185 876 343 185 124 98 12 8 Bpu10I CC'TnA_GC Cuts at: 3101 3809 3867 4651 3101 Size: 708 58 784 6206 Fragments arranged by size: 6206 784 708 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2562 3194 3917 3923 5881 6746 7044 2562 Size: 632 723 6 1958 865 298 3274 Fragments arranged by size: 3274 1958 865 723 632 298 6 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2427 2624 3813 4736 6419 882 Size: 617 928 197 1189 923 1683 2219 Fragments arranged by size: 2219 1683 1189 928 923 617 197 BsaAI yAC'GTr Cuts at: 493 1204 2307 5547 493 Size: 711 1103 3240 2702 Fragments arranged by size: 3240 2702 1103 711 BsaBI GATnn'nnATC Cuts at: 7750 7750 Size: 7756 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3543 4638 5563 5945 Size: 53 83 186 936 2010 1095 925 382 Cuts at: 5945 6580 275 Size: 635 1451 Fragments arranged by size: 2010 1451 1095 936 925 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2385 2805 4897 6189 6587 6861 7008 7419 2385 Size: 420 2092 1292 398 274 147 411 2722 Fragments arranged by size: 2722 2092 1292 420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3619 3649 4004 4034 1178 Size: 30 165 30 2216 30 355 30 4900 Fragments arranged by size: 4900 2216 355 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3122 3125 4295 4811 1186 Size: 1936 3 1170 516 4131 Fragments arranged by size: 4131 1936 1170 516 3 BseYI C'CCAG_C Cuts at: 2318 3025 3171 6959 7455 2318 Size: 707 146 3788 496 2619 Fragments arranged by size: 3788 2619 707 496 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4014 4626 4754 1163 Size: 573 2278 612 128 4165 Fragments arranged by size: 4165 2278 612 573 128 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2960 5206 5967 6995 1540 Size: 275 186 959 2246 761 1028 2301 Fragments arranged by size: 2301 2246 1028 959 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3742 4652 5817 5902 6973 3742 Size: 910 1165 85 1071 4525 Fragments arranged by size: 4525 1165 1071 910 85 BsmI GAATG_Cn' Cuts at: 2485 3062 3123 3434 3628 4076 5267 5360 2485 Size: 577 61 311 194 448 1191 93 4881 Fragments arranged by size: 4881 1191 577 448 311 194 93 61 BsmBI CGTCTCn'nnnn_ Cuts at: 3962 4190 4307 3962 Size: 228 117 7411 Fragments arranged by size: 7411 228 117 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2805 3278 3464 3476 3742 4652 5075 Size: 399 1015 473 186 12 266 910 423 Cuts at: 5075 5817 5902 6973 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1015 910 742 473 423 399 266 186 85 12 BspEI T'CCGG_A Cuts at: 2385 7419 2385 Size: 5034 2722 BspHI T'CATG_A Cuts at: 3159 5645 7375 3159 Size: 2486 1730 3540 Fragments arranged by size: 3540 2486 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3598 5111 844 Size: 2754 1513 3489 Fragments arranged by size: 3489 2754 1513 BsrBI CCG'CTC Cuts at: 1394 1823 2925 3718 5643 1394 Size: 429 1102 793 1925 3507 Fragments arranged by size: 3507 1925 1102 793 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4345 6251 6425 65 Size: 1183 3097 1906 174 1396 Fragments arranged by size: 3097 1906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6400 6587 1700 Size: 110 4590 187 2869 Fragments arranged by size: 4590 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4110 96 Size: 4014 3742 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7756 BssSI C'ACGA_G Cuts at: 4802 5816 6828 4802 Size: 1014 1012 5730 Fragments arranged by size: 5730 1014 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7756 BstBI TT'CG_AA Cuts at: 6560 6560 Size: 7756 BstEII G'GTnAC_C Cuts at: 1184 2694 1184 Size: 1510 6246 BstXI CCAn_nnnn'nTGG Cuts at: 3271 3271 Size: 7756 BstYI r'GATC_y Cuts at: 1972 4215 4482 5086 5453 5838 5855 7307 7393 Size: 2243 267 604 367 385 17 1452 86 Cuts at: 7393 7405 7751 1972 Size: 12 346 1977 Fragments arranged by size: 2243 1977 1452 604 385 367 346 267 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5045 5045 Size: 7756 BtgI C'CryG_G Cuts at: 513 1065 3429 513 Size: 552 2364 4840 Fragments arranged by size: 4840 2364 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2415 3861 487 Size: 683 56 1189 1446 4382 Fragments arranged by size: 4382 1446 1189 683 56 BtsI GCAGTG_nn' Cuts at: 2369 2748 3844 4221 5272 6066 6086 2369 Size: 379 1096 377 1051 794 20 4039 Fragments arranged by size: 4039 1096 1051 794 379 377 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7756 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4920 4955 529 Size: 35 4356 35 3330 Fragments arranged by size: 4356 3330 35 35 DraI TTT'AAA Cuts at: 5060 5060 Size: 7756 DraIII CAC_nnn'GTG Cuts at: 3737 5022 3737 Size: 1285 6471 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6763 809 Size: 507 5447 1802 Fragments arranged by size: 5447 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2957 3373 5203 Size: 54 1425 50 275 18 1127 416 1830 Cuts at: 5203 6092 7721 8 Size: 889 1629 43 Fragments arranged by size: 1830 1629 1425 1127 889 416 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2957 5203 1537 Size: 275 1145 2246 4090 Fragments arranged by size: 4090 2246 1145 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3110 3245 3490 4428 4718 4946 5018 5686 Size: 1757 135 245 938 290 228 72 668 Cuts at: 5686 1353 Size: 3423 Fragments arranged by size: 3423 1757 938 668 290 245 228 135 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6343 6717 6863 387 Size: 1064 138 4754 374 146 1280 Fragments arranged by size: 4754 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2115 2127 2135 2320 3505 3848 Size: 393 404 79 12 8 185 1185 343 Cuts at: 3848 3972 4067 4070 4187 4985 5819 6416 7203 Size: 124 95 3 117 798 834 597 787 Cuts at: 7203 7471 1239 Size: 268 1524 Fragments arranged by size: 1524 1185 834 798 787 597 404 393 343 268 185 124 117 95 79 12 8 3 EcoNI CCTnn'n_nnAGG Cuts at: 2153 3104 2153 Size: 951 6805 EcoO109I rG'GnC_Cy Cuts at: 2551 2744 3813 2551 Size: 193 1069 6494 Fragments arranged by size: 6494 1069 193 EcoRI G'AATT_C Cuts at: 5065 5443 5065 Size: 378 7378 EcoRV GAT'ATC Cuts at: 3325 3325 Size: 7756 FspI TGC'GCA Cuts at: 6262 6262 Size: 7756 HaeII r_GCGC'y Cuts at: 1678 3546 4373 5011 6903 1678 Size: 1868 827 638 1892 2531 Fragments arranged by size: 2531 1892 1868 827 638 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2191 2223 6397 6429 6471 Size: 32 135 32 619 32 4174 32 42 Cuts at: 6471 6503 1373 Size: 32 2626 Fragments arranged by size: 4174 2626 619 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2032 2544 3304 3663 4932 5100 Size: 988 281 94 512 760 359 1269 168 Cuts at: 5100 5346 669 Size: 246 3079 Fragments arranged by size: 3079 1269 988 760 512 359 281 246 168 94 HindIII A'AGCT_T Cuts at: 748 2345 748 Size: 1597 6159 HpaI GTT'AAC Cuts at: 3663 5346 3663 Size: 1683 6073 KasI G'GCGC_C Cuts at: 3542 3542 Size: 7756 KpnI G_GTAC'C Cuts at: 4840 5081 4840 Size: 241 7515 MfeI C'AATT_G Cuts at: 5355 5355 Size: 7756 MluI A'CGCG_T Cuts at: 7632 7632 Size: 7756 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 3788 4710 6870 7054 1305 Size: 1302 1181 922 2160 184 2007 Fragments arranged by size: 2160 2007 1302 1181 922 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5934 Fragments arranged by size: 5934 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3965 4118 5714 6073 6232 518 Size: 648 2799 153 1596 359 159 2042 Fragments arranged by size: 2799 2042 1596 648 359 159 153 MspA1I CmG'CkG Cuts at: 1606 2691 2962 3241 3409 3770 3992 4236 4379 Size: 1085 271 279 168 361 222 244 143 Cuts at: 4379 5172 5849 6997 7242 1606 Size: 793 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 793 677 361 279 271 245 244 222 168 143 NarI GG'CG_CC Cuts at: 3543 3543 Size: 7756 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7204 NdeI CA'TA_TG Cuts at: 387 387 Size: 7756 NheI G'CTAG_C Cuts at: 1052 2440 1052 Size: 1388 6368 NotI GC'GGCC_GC Cuts at: 5203 5203 Size: 7756 NspI r_CATG'y Cuts at: 1132 1229 2220 2949 5114 6659 1132 Size: 97 991 729 2165 1545 2229 Fragments arranged by size: 2229 2165 1545 991 729 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7756 PciI A'CATG_T Cuts at: 2945 6655 2945 Size: 3710 4046 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7756 PfoI T'CCnGG_A Cuts at: 2151 3008 3825 2151 Size: 857 817 6082 Fragments arranged by size: 6082 857 817 PmeI GTTT'AAAC Cuts at: 5060 5060 Size: 7756 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5825 5857 7338 7370 5825 Size: 32 1481 32 6211 Fragments arranged by size: 6211 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2551 2551 Size: 7756 PshAI GACnn'nnGTC Cuts at: 1613 2027 3954 1613 Size: 414 1927 5415 Fragments arranged by size: 5415 1927 414 PsiI TTA'TAA Cuts at: 5326 5326 Size: 7756 PstI C_TGCA'G Cuts at: 830 3595 4384 4794 5108 830 Size: 2765 789 410 314 3478 Fragments arranged by size: 3478 2765 789 410 314 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7756 PvuII CAG'CTG Cuts at: 2691 3241 3409 3770 3992 4236 2691 Size: 550 168 361 222 244 6211 Fragments arranged by size: 6211 550 361 244 222 168 SalI G'TCGA_C Cuts at: 1936 5098 1936 Size: 3162 4594 SanDI GG'GwC_CC Cuts at: 2551 2551 Size: 7756 SapI GCTCTTCn'nnn_ Cuts at: 3110 4718 3110 Size: 1608 6148 SbfI CC_TGCA'GG Cuts at: 3595 5108 3595 Size: 1513 6243 ScaI AGT'ACT Cuts at: 6004 7716 6004 Size: 1712 6044 SexAI A'CCwGG_T Cuts at: 2712 3733 4569 4767 2712 Size: 1021 836 198 5701 Fragments arranged by size: 5701 1021 836 198 SfcI C'TryA_G Cuts at: 826 1045 3591 4380 4790 5104 6239 6920 7111 Size: 219 2546 789 410 314 1135 681 191 Cuts at: 7111 826 Size: 1471 Fragments arranged by size: 2546 1471 1135 789 681 410 314 219 191 SfoI GGC'GCC Cuts at: 3544 3544 Size: 7756 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7756 SmaI CCC'GGG Cuts at: 1872 5083 1872 Size: 3211 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2577 3209 3896 3902 5860 6761 Size: 197 926 634 632 687 6 1958 901 Cuts at: 6761 7023 820 Size: 262 1553 Fragments arranged by size: 1958 1553 926 901 687 634 632 262 197 6 SnaBI TAC'GTA Cuts at: 493 2307 5547 493 Size: 1814 3240 2702 Fragments arranged by size: 3240 2702 1814 SpeI A'CTAG_T Cuts at: 152 152 Size: 7756 SphI G_CATG'C Cuts at: 1132 5114 1132 Size: 3982 3774 SspI AAT'ATT Cuts at: 5 52 2179 5680 5 Size: 47 2127 3501 2081 Fragments arranged by size: 3501 2127 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7756 StyI C'CwwG_G Cuts at: 513 1065 5195 513 Size: 552 4130 3074 Fragments arranged by size: 4130 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3179 5783 5800 5953 6138 6583 325 Size: 1255 1599 2604 17 153 185 445 1498 Fragments arranged by size: 2604 1599 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4110 6002 7714 Size: 274 80 33 51 1443 2133 1892 1712 Cuts at: 7714 96 Size: 138 Fragments arranged by size: 2133 1892 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4004 4036 7716 7748 4004 Size: 32 3680 32 4012 Fragments arranged by size: 4012 3680 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3670 1316 Size: 2354 5402 XbaI T'CTAG_A Cuts at: 5092 5092 Size: 7756 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3086 3137 4567 3086 Size: 51 1430 6275 Fragments arranged by size: 6275 1430 51 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7756 XmaI C'CCGG_G Cuts at: 1870 5081 1870 Size: 3211 4545 XmnI GAAnn'nnTTC Cuts at: 2178 5885 2178 Size: 3707 4049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5564 276 Size: 53 83 186 936 4030 2468 Fragments arranged by size: 4030 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI BstZ17I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI