(Circular) (Six-base) MAPSORT of: pFN21AB1986.seq Check: 9496 from: 1 to: 8014 pFN21AB1986 8014 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5824 278 Size: 53 83 186 936 4288 2468 Fragments arranged by size: 4288 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3249 5357 1937 Size: 1312 2108 4594 Fragments arranged by size: 4594 2108 1312 Acc65I G'GTAC_C Cuts at: 5335 5335 Size: 8014 AclI AA'CG_TT Cuts at: 1563 4224 6141 6514 1563 Size: 2661 1917 373 3063 Fragments arranged by size: 3063 2661 1917 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3293 3948 4517 4731 6077 7461 7729 1632 Size: 1661 655 569 214 1346 1384 268 1917 Fragments arranged by size: 1917 1661 1384 1346 655 569 268 214 AflII C'TTAA_G Cuts at: 820 1017 4325 820 Size: 197 3308 4509 Fragments arranged by size: 4509 3308 197 AflIII A'CryG_T Cuts at: 1203 4723 6913 7890 1203 Size: 3520 2190 977 1327 Fragments arranged by size: 3520 2190 1327 977 AgeI A'CCGG_T Cuts at: 6845 6845 Size: 8014 AhdI GACnn_n'nnGTC Cuts at: 6743 6743 Size: 8014 AleI CACnn'nnGTG Cuts at: 1166 3405 1166 Size: 2239 5775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4680 1184 Size: 3496 4518 AlwNI CAG_nnn'CTG Cuts at: 4174 5757 7329 4174 Size: 1583 1572 4859 Fragments arranged by size: 4859 1583 1572 ApaLI G'TGCA_C Cuts at: 6071 7227 6071 Size: 1156 6858 ApoI r'AATT_y Cuts at: 1493 2302 3080 3545 3666 4054 4146 4703 4755 Size: 809 778 465 121 388 92 557 52 Cuts at: 4755 4820 5323 5549 5701 7810 1493 Size: 65 503 226 152 2109 1697 Fragments arranged by size: 2109 1697 809 778 557 503 465 388 226 152 121 92 65 52 AseI AT'TA_AT Cuts at: 160 1032 6568 160 Size: 872 5536 1606 Fragments arranged by size: 5536 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8014 AvaI C'yCGr_G Cuts at: 1870 1943 2015 4887 5339 1870 Size: 73 72 2872 452 4545 Fragments arranged by size: 4545 2872 452 73 72 BamHI G'GATC_C Cuts at: 3415 4153 5344 5711 3415 Size: 738 1191 367 5718 Fragments arranged by size: 5718 1191 738 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5335 6790 618 Size: 325 217 627 3548 1455 1842 Fragments arranged by size: 3548 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3751 4382 5333 1391 Size: 2360 631 951 4072 Fragments arranged by size: 4072 2360 951 631 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2502 4017 4115 4218 4251 5084 928 Size: 971 603 1515 98 103 33 833 3858 Fragments arranged by size: 3858 1515 971 833 603 103 98 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6205 6239 1903 Size: 34 4268 34 3678 Fragments arranged by size: 4268 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3786 4424 5906 7116 3786 Size: 638 1482 1210 4684 Fragments arranged by size: 4684 1482 1210 638 BclI T'GATC_A Cuts at: 1548 1548 Size: 8014 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4046 6625 136 Size: 107 122 71 631 605 2374 2579 1525 Fragments arranged by size: 2579 2374 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3115 4030 8009 3115 Size: 915 3979 3120 Fragments arranged by size: 3979 3120 915 BlpI GC'TnA_GC Cuts at: 2274 5431 2274 Size: 3157 4857 Bme1580I G_kGCm'C Cuts at: 1790 6075 7231 1790 Size: 4285 1156 2573 Fragments arranged by size: 4285 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8014 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4122 4573 5745 Size: 416 53 83 399 24 2705 451 1172 Cuts at: 5745 6703 442 Size: 958 1753 Fragments arranged by size: 2705 1753 1172 958 451 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8014 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4253 6674 1239 Size: 3014 2421 2579 Fragments arranged by size: 3014 2579 2421 Bpu10I CC'TnA_GC Cuts at: 4293 4293 Size: 8014 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2229 3552 3637 4552 4745 6139 7004 7302 2229 Size: 1323 85 915 193 1394 865 298 2941 Fragments arranged by size: 2941 1394 1323 915 865 298 193 85 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5229 6677 882 Size: 617 3730 1448 2219 Fragments arranged by size: 3730 2219 1448 617 BsaAI yAC'GTr Cuts at: 493 1204 4726 5805 493 Size: 711 3522 1079 2702 Fragments arranged by size: 3522 2702 1079 711 BsaBI GATnn'nnATC Cuts at: 8008 8008 Size: 8014 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 5821 6203 6838 Size: 53 83 186 936 494 3794 382 635 Cuts at: 6838 275 Size: 1451 Fragments arranged by size: 3794 1451 936 635 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2605 6447 6845 7119 7266 7677 2605 Size: 3842 398 274 147 411 2942 Fragments arranged by size: 3842 2942 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7789 Fragments arranged by size: 7789 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3741 3750 3759 3825 3855 3870 1186 Size: 2555 9 9 66 30 15 5330 Fragments arranged by size: 5330 2555 66 30 15 9 9 BseYI C'CCAG_C Cuts at: 2613 7217 7713 2613 Size: 4604 496 2914 Fragments arranged by size: 4604 2914 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5244 1163 Size: 573 3508 3933 Fragments arranged by size: 3933 3508 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2540 5464 6225 7253 1540 Size: 275 186 539 2924 761 1028 2301 Fragments arranged by size: 2924 2301 1028 761 539 275 186 BsiHKAI G_wGCw'C Cuts at: 3751 4382 6075 6160 7231 3751 Size: 631 1693 85 1071 4534 Fragments arranged by size: 4534 1693 1071 631 85 BsmI GAATG_Cn' Cuts at: 2504 5228 5525 5618 2504 Size: 2724 297 93 4900 Fragments arranged by size: 4900 2724 297 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3212 4409 3212 Size: 1197 6817 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3751 4382 5333 6075 6160 7231 1391 Size: 399 1961 631 951 742 85 1071 2174 Fragments arranged by size: 2174 1961 1071 951 742 631 399 85 BspEI T'CCGG_A Cuts at: 7677 7677 Size: 8014 BspHI T'CATG_A Cuts at: 2527 5903 7633 2527 Size: 3376 1730 2908 Fragments arranged by size: 3376 2908 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5369 844 Size: 4525 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2036 5901 1394 Size: 429 213 3865 3507 Fragments arranged by size: 3865 3507 429 213 BsrDI GCAATG_nn' Cuts at: 65 1248 3692 6509 6683 65 Size: 1183 2444 2817 174 1396 Fragments arranged by size: 2817 2444 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6658 6845 1700 Size: 110 4848 187 2869 Fragments arranged by size: 4848 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8014 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8014 BssSI C'ACGA_G Cuts at: 6074 7086 6074 Size: 1012 7002 BstAPI GCAn_nnn'nTGC Cuts at: 794 5096 794 Size: 4302 3712 BstBI TT'CG_AA Cuts at: 3061 3631 6818 3061 Size: 570 3187 4257 Fragments arranged by size: 4257 3187 570 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8014 BstXI CCAn_nnnn'nTGG Cuts at: 4186 4186 Size: 8014 BstYI r'GATC_y Cuts at: 1972 2326 3115 3415 4030 4153 5344 5711 6096 Size: 354 789 300 615 123 1191 367 385 Cuts at: 6096 6113 7565 7651 7663 8009 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1191 789 615 385 367 354 346 300 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2582 2582 Size: 8014 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6523 Fragments arranged by size: 6523 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2267 487 Size: 683 56 1041 6234 Fragments arranged by size: 6234 1041 683 56 BtsI GCAGTG_nn' Cuts at: 2439 3279 3400 3412 5046 5530 6324 6344 2439 Size: 840 121 12 1634 484 794 20 4109 Fragments arranged by size: 4109 1634 840 794 484 121 20 12 ClaI AT'CG_AT Cuts at: 1554 3450 1554 Size: 1896 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7979 DraI TTT'AAA Cuts at: 5318 5318 Size: 8014 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7021 809 Size: 507 5705 1802 Fragments arranged by size: 5705 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2629 5461 6350 Size: 54 1425 50 275 18 799 2832 889 Cuts at: 6350 7979 8 Size: 1629 43 Fragments arranged by size: 2832 1629 1425 889 799 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5461 1537 Size: 275 3649 4090 Fragments arranged by size: 4090 3649 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2361 2880 3759 3935 4986 5056 5067 Size: 750 258 519 879 176 1051 70 11 Cuts at: 5067 5944 1353 Size: 877 3423 Fragments arranged by size: 3423 1051 879 877 750 519 258 176 70 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6601 6975 7121 387 Size: 1064 138 5012 374 146 1280 Fragments arranged by size: 5012 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3749 4380 3749 Size: 631 7383 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3293 3948 4253 4517 4731 6077 6674 Size: 393 1661 655 305 264 214 1346 597 Cuts at: 6674 7461 7729 1239 Size: 787 268 1524 Fragments arranged by size: 1661 1524 1346 787 655 597 393 305 268 264 214 EcoNI CCTnn'n_nnAGG Cuts at: 3870 3870 Size: 8014 EcoO109I rG'GnC_Cy Cuts at: 3674 3830 3674 Size: 156 7858 EcoRI G'AATT_C Cuts at: 3080 3545 3666 5323 5701 3080 Size: 465 121 1657 378 5393 Fragments arranged by size: 5393 1657 465 378 121 EcoRV GAT'ATC Cuts at: 4696 4696 Size: 8014 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3593 4618 3593 Size: 1025 6989 FspI TGC'GCA Cuts at: 6520 6520 Size: 8014 HaeII r_GCGC'y Cuts at: 1678 7161 1678 Size: 5483 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2279 2311 3432 3464 4581 Size: 32 135 32 707 32 1121 32 1117 Cuts at: 4581 4613 4645 5182 5214 6655 6687 6729 6761 Size: 32 32 537 32 1441 32 42 32 Cuts at: 6761 1373 Size: 2626 Fragments arranged by size: 2626 1441 1121 1117 707 537 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2620 5358 5604 669 Size: 988 281 682 2738 246 3079 Fragments arranged by size: 3079 2738 988 682 281 246 HindIII A'AGCT_T Cuts at: 748 2669 4510 748 Size: 1921 1841 4252 Fragments arranged by size: 4252 1921 1841 HpaI GTT'AAC Cuts at: 2620 5604 2620 Size: 2984 5030 KpnI G_GTAC'C Cuts at: 5339 5339 Size: 8014 MfeI C'AATT_G Cuts at: 2814 2961 3949 5613 2814 Size: 147 988 1664 5215 Fragments arranged by size: 5215 1664 988 147 MluI A'CGCG_T Cuts at: 7890 7890 Size: 8014 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2437 2860 4525 7128 7312 1305 Size: 1132 423 1665 2603 184 2007 Fragments arranged by size: 2603 2007 1665 1132 423 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2631 10 Size: 54 1425 343 799 5393 Fragments arranged by size: 5393 1425 799 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3405 5280 5972 6331 6490 518 Size: 648 2239 1875 692 359 159 2042 Fragments arranged by size: 2239 2042 1875 692 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2613 4634 5430 6107 7255 7500 1606 Size: 1007 2021 796 677 1148 245 2120 Fragments arranged by size: 2120 2021 1148 1007 796 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6523 Fragments arranged by size: 6523 939 552 NdeI CA'TA_TG Cuts at: 387 4207 387 Size: 3820 4194 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8014 NotI GC'GGCC_GC Cuts at: 5461 5461 Size: 8014 NruI TCG'CGA Cuts at: 2252 2252 Size: 8014 NspI r_CATG'y Cuts at: 1132 1229 3016 4204 4252 5372 6917 1132 Size: 97 1787 1188 48 1120 1545 2229 Fragments arranged by size: 2229 1787 1545 1188 1120 97 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8014 PciI A'CATG_T Cuts at: 6913 6913 Size: 8014 PflMI CCAn_nnn'nTGG Cuts at: 1108 3851 4585 1108 Size: 2743 734 4537 Fragments arranged by size: 4537 2743 734 PmeI GTTT'AAAC Cuts at: 5318 5318 Size: 8014 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6083 6115 7596 7628 4550 Size: 32 1501 32 1481 32 4936 Fragments arranged by size: 4936 1501 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3674 3830 3674 Size: 156 7858 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8014 PsiI TTA'TAA Cuts at: 3023 3363 5584 3023 Size: 340 2221 5453 Fragments arranged by size: 5453 2221 340 PstI C_TGCA'G Cuts at: 830 2272 3407 3520 3685 5366 830 Size: 1442 1135 113 165 1681 3478 Fragments arranged by size: 3478 1681 1442 1135 165 113 PvuI CG_AT'CG Cuts at: 2001 2540 2001 Size: 539 7475 PvuII CAG'CTG Cuts at: 2613 2613 Size: 8014 SacI G_AGCT'C Cuts at: 3751 4382 3751 Size: 631 7383 SalI G'TCGA_C Cuts at: 1936 5356 1936 Size: 3420 4594 SbfI CC_TGCA'GG Cuts at: 5366 5366 Size: 8014 ScaI AGT'ACT Cuts at: 3648 6262 7974 3648 Size: 2614 1712 3688 Fragments arranged by size: 3688 2614 1712 SfcI C'TryA_G Cuts at: 826 1045 2268 2451 3403 3516 3681 5362 6497 Size: 219 1223 183 952 113 165 1681 1135 Cuts at: 6497 7178 7369 826 Size: 681 191 1471 Fragments arranged by size: 1681 1471 1223 1135 952 681 219 191 183 165 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8014 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8014 SmaI CCC'GGG Cuts at: 1872 2017 5341 1872 Size: 145 3324 4545 Fragments arranged by size: 4545 3324 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 2244 3567 3652 4325 4567 4760 Size: 197 926 301 1323 85 673 242 193 Cuts at: 4760 6118 7019 7281 820 Size: 1358 901 262 1553 Fragments arranged by size: 1553 1358 1323 926 901 673 301 262 242 197 193 85 SnaBI TAC'GTA Cuts at: 493 5805 493 Size: 5312 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8014 SphI G_CATG'C Cuts at: 1132 3016 4204 5372 1132 Size: 1884 1188 1168 3774 Fragments arranged by size: 3774 1884 1188 1168 SspI AAT'ATT Cuts at: 5 52 2698 3359 5938 5 Size: 47 2646 661 2579 2081 Fragments arranged by size: 2646 2579 2081 661 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8014 StyI C'CwwG_G Cuts at: 513 1065 2004 3850 5255 5453 513 Size: 552 939 1846 1405 198 3074 Fragments arranged by size: 3074 1846 1405 939 552 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2190 6041 6058 6211 6396 6841 325 Size: 1255 610 3851 17 153 185 445 1498 Fragments arranged by size: 3851 1498 1255 610 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3646 6260 7972 Size: 274 80 33 51 1443 1669 2614 1712 Cuts at: 7972 96 Size: 138 Fragments arranged by size: 2614 1712 1669 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7974 8006 7974 Size: 32 7982 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8014 XbaI T'CTAG_A Cuts at: 2896 5203 5350 2896 Size: 2307 147 5560 Fragments arranged by size: 5560 2307 147 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2413 2639 2413 Size: 226 7788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8014 XmaI C'CCGG_G Cuts at: 1870 2015 5339 1870 Size: 145 3324 4545 Fragments arranged by size: 4545 3324 145 XmnI GAAnn'nnTTC Cuts at: 2523 2670 3080 3989 6143 2523 Size: 147 410 909 2154 4394 Fragments arranged by size: 4394 2154 909 410 147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5822 276 Size: 53 83 186 936 4288 2468 Fragments arranged by size: 4288 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI