Restriction map for
pFN21AB1630
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII AccIII AclI AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AspEI AspI AviII BalI BamHI
BbrPI BcgI BclI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BsgI
BsiWI Bsp1407I BspEI BspHI BsrBI
BsrFI BsrGI BstAPI BstEII BstPI
BstXI Bsu36I CelII Cfr10I CpoI
DraI DraII DrdI Eam1105I Eco81I
EcoNI EcoO109I EcoO65I FbaI FseI
FspI HincII HindII HpaI MluNI
MroI MscI NaeI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I RcaI
RsrII SalI SbfI SfiI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflII 2965 CTTAAG C^TTAAG
Aor51HI 148 AGCGCT AGC^GCT
AseI 1281 ATTAAT AT^TAAT
AsnI 1281 ATTAAT AT^TAAT
AvaI 37 CCCGAG C^[CT]CG[GA]G
AvrII 624 CCTAGG C^CTAGG
BbeI 368 GGCGCC GGCGC^C
BbvCI 1245 CCTCAGC CCTCAGC(-5/-2)
BfrI 2965 CTTAAG C^TTAAG
BglI 107 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 624 CCTAGG C^CTAGG
BsiYI 74 CCAGCGGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 74 CCAGCGGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 815 GAGACG CGTCTC(1/5)
BsmFI 155 GTCCC GGGAC(10/14)
BsmI 2176 GCATTC GAATGC(1/-1)
BsoBI 37 CCCGAG C^[CT]CG[GA]G
BspDI 2020 ATCGAT AT^CGAT
BspLU11I 1977 ACATGT A^CATGT
BsrDI 2980 GCAATG GCAATG(2/0)
BssHII 44 GCGCGC G^CGCGC
BssSI 1499 CACGAG CACGAG(-5/-1)
Bst1107I 1269 GTATAC GTA^TAC
BstBI 2140 TTCGAA TT^CGAA
BstZ17I 1269 GTATAC GTA^TAC
ClaI 2020 ATCGAT AT^CGAT
DraIII 2181 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 8 CCGCGG C^C[GA][CT]GG
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco47III 148 AGCGCT AGC^GCT
Eco52I 11 CGGCCG C^GGCCG
EcoRV 2017 GATATC GAT^ATC
HindIII 2506 AAGCTT A^AGCTT
KasI 368 GGCGCC G^GCGCC
KspI 8 CCGCGG CCGC^GG
MfeI 2529 CAATTG C^AATTG
MluI 1675 ACGCGT A^CGCGT
MslI 1344 CATCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2529 CAATTG C^AATTG
NarI 368 GGCGCC GG^CGCC
NspV 2140 TTCGAA TT^CGAA
PshBI 1281 ATTAAT AT^TAAT
PvuI 68 CGATCG CGAT^CG
SacII 8 CCGCGG CCGC^GG
SexAI 1401 ACCTGGT A^CC[AT]GGT
SfoI 368 GGCGCC GGC^GCC
SfuI 2140 TTCGAA TT^CGAA
SmlI 2965 CTTAAG C^T[CT][GA]AG
SpeI 1137 ACTAGT A^CTAGT
XcmI 2972 CCAGAACTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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Acc65I 753 GGTACC G^GTACC
2397 GGTACC
AccI 714 GTCTAC GT^[AC][GT]AC
1269 GTATAC
AflIII 1675 ACGCGT A^C[GA][CT]GT
1977 ACATGT
AlwNI 1397 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1918 CAGAATCTG
Asp700I 1945 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2248 GAAGCTCTTC
Asp718I 753 GGTACC G^GTACC
2397 GGTACC
BanII 2677 GAGCTC G[GA]GC[CT]^C
2986 GAGCTC
BciVI 2115 GGATAC GTATCC(6/5)
602 GTATCC
BcnI 261 CCGGG CC^[GC]GG
829 CCGGG
BglII 766 AGATCT A^GATCT
1755 AGATCT
BsiEI 11 CGGCCG CG[GA][CT]^CG
68 CGATCG
BsmAI 815 GAGAC GTCTC(1/5)
1611 GAGAC
BspMI 321 ACCTGC ACCTGC(4/8)
1580 GCAGGT
EaeI 11 CGGCCG [CT]^GGCC[GA]
143 CGGCCA
HaeII 148 AGCGCT [GA]GCGC^[CT]
368 GGCGCC
HapII 261 CCGG C^CGG
829 CCGG
HpaII 261 CCGG C^CGG
829 CCGG
HphI 196 TCACC GGTGA(8/7)
346 TCACC
KpnI 753 GGTACC GGTAC^C
2397 GGTACC
MspI 261 CCGG C^CGG
829 CCGG
NciI 261 CCGGG CC^[GC]GG
829 CCGGG
NspI 1556 GCATGT [GA]CATG^[CT]
1977 ACATGT
PstI 1578 CTGCAG CTGCA^G
2054 CTGCAG
PvuII 2651 CAGCTG CAG^CTG
2800 CAGCTG
SacI 2677 GAGCTC GAGCT^C
2986 GAGCTC
SapI 454 GAAGAGC GCTCTTC(1/4)
2251 GCTCTTC
ScaI 1503 AGTACT AGT^ACT
1574 AGTACT
SspI 180 AATATT AAT^ATT
2645 AATATT
Tsp45I 195 GTCAC ^GT[GC]AC
1044 GTCAC
XmnI 1945 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2248 GAAGCTCTTC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BseRI BsiHKAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HgaI HhaI
Hin1I HinP1I HinfI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MspA1I
MvaI MvnI MwoI NdeI NdeII
NlaIII NlaIV NsiI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII