(Circular) (Six-base) MAPSORT of: pFN21AB1630.seq Check: 9323 from: 1 to: 7861 pFN21AB1630 7861 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5671 278 Size: 53 83 186 936 4135 2468 Fragments arranged by size: 4135 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2720 3275 5204 1937 Size: 783 555 1929 4594 Fragments arranged by size: 4594 1929 783 555 Acc65I G'GTAC_C Cuts at: 2758 4402 5182 2758 Size: 1644 780 5437 Fragments arranged by size: 5437 1644 780 AclI AA'CG_TT Cuts at: 1563 5988 6361 1563 Size: 4425 373 3063 Fragments arranged by size: 4425 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3008 3502 3749 4869 5924 7308 7576 1632 Size: 1376 494 247 1120 1055 1384 268 1917 Fragments arranged by size: 1917 1384 1376 1120 1055 494 268 247 AfeI AGC'GCT Cuts at: 2155 2155 Size: 7861 AflII C'TTAA_G Cuts at: 820 1017 4970 820 Size: 197 3953 3711 Fragments arranged by size: 3953 3711 197 AflIII A'CryG_T Cuts at: 1203 3680 3982 6760 7737 1203 Size: 2477 302 2778 977 1327 Fragments arranged by size: 2778 2477 1327 977 302 AgeI A'CCGG_T Cuts at: 6692 6692 Size: 7861 AhdI GACnn_n'nnGTC Cuts at: 6590 6590 Size: 7861 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7861 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2154 1184 Size: 970 6891 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2396 2428 4313 4345 2396 Size: 32 1885 32 5912 Fragments arranged by size: 5912 1885 32 32 AlwNI CAG_nnn'CTG Cuts at: 3407 3928 5604 7176 3407 Size: 521 1676 1572 4092 Fragments arranged by size: 4092 1676 1572 521 ApaLI G'TGCA_C Cuts at: 5918 7074 5918 Size: 1156 6705 ApoI r'AATT_y Cuts at: 1493 2744 2900 2981 4368 4734 5170 5396 5548 Size: 1251 156 81 1387 366 436 226 152 Cuts at: 5548 7657 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1387 1251 436 366 226 156 152 81 AseI AT'TA_AT Cuts at: 160 1032 3287 6415 160 Size: 872 2255 3128 1606 Fragments arranged by size: 3128 2255 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7861 AvaI C'yCGr_G Cuts at: 1870 1943 2042 5186 1870 Size: 73 99 3144 4545 Fragments arranged by size: 4545 3144 99 73 AvrII C'CTAG_G Cuts at: 2629 2629 Size: 7861 BamHI G'GATC_C Cuts at: 5191 5558 5191 Size: 367 7494 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2373 2758 4402 5182 6637 Size: 325 217 627 586 385 1644 780 1455 Cuts at: 6637 618 Size: 1842 Fragments arranged by size: 1842 1644 1455 780 627 586 385 325 217 BanII G_rGCy'C Cuts at: 1391 4686 4995 5180 1391 Size: 3295 309 185 4072 Fragments arranged by size: 4072 3295 309 185 BbeI G_GCGC'C Cuts at: 2377 2377 Size: 7861 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2345 2517 4061 4161 928 Size: 971 446 172 1544 100 4628 Fragments arranged by size: 4628 1544 971 446 172 100 BbvCI CC'TCA_GC Cuts at: 3251 3251 Size: 7861 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6052 6086 1903 Size: 34 4115 34 3678 Fragments arranged by size: 4115 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2618 4114 5753 6963 2618 Size: 1496 1639 1210 3516 Fragments arranged by size: 3516 1639 1496 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7861 BfrBI ATG'CAT Cuts at: 3433 3635 4241 3433 Size: 202 606 7053 Fragments arranged by size: 7053 606 202 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2118 6472 136 Size: 107 122 71 631 605 446 4354 1525 Fragments arranged by size: 4354 1525 631 605 446 122 107 71 BglII A'GATC_T Cuts at: 2771 3760 7856 2771 Size: 989 4096 2776 Fragments arranged by size: 4096 2776 989 BlpI GC'TnA_GC Cuts at: 5278 5278 Size: 7861 Bme1580I G_kGCm'C Cuts at: 1790 5922 7078 1790 Size: 4132 1156 2573 Fragments arranged by size: 4132 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7861 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5592 6550 442 Size: 416 53 83 399 24 4175 958 1753 Fragments arranged by size: 4175 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7861 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3234 3234 Size: 7861 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4617 4670 4979 6521 1239 Size: 3378 53 309 1542 2579 Fragments arranged by size: 3378 2579 1542 309 53 Bpu10I CC'TnA_GC Cuts at: 3251 4416 3251 Size: 1165 6696 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5986 6851 7149 5986 Size: 865 298 6698 Fragments arranged by size: 6698 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6524 882 Size: 617 5025 2219 Fragments arranged by size: 5025 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5652 493 Size: 711 4448 2702 Fragments arranged by size: 4448 2702 711 BsaBI GATnn'nnATC Cuts at: 3432 3747 3924 7855 3432 Size: 315 177 3931 3438 Fragments arranged by size: 3931 3438 315 177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2169 2374 5668 Size: 53 83 186 936 588 48 205 3294 Cuts at: 5668 6050 6685 275 Size: 382 635 1451 Fragments arranged by size: 3294 1451 936 635 588 382 205 186 83 53 48 BsaWI w'CCGG_w Cuts at: 6294 6692 6966 7113 7524 6294 Size: 398 274 147 411 6631 Fragments arranged by size: 6631 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7636 Fragments arranged by size: 7636 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 4281 4935 5014 1186 Size: 1279 1816 654 79 4033 Fragments arranged by size: 4033 1816 1279 654 79 BseYI C'CCAG_C Cuts at: 7064 7560 7064 Size: 496 7365 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2076 5311 6072 7100 1540 Size: 275 186 18 57 3235 761 1028 2301 Fragments arranged by size: 3235 2301 1028 761 275 186 57 18 BsiHKAI G_wGCw'C Cuts at: 3784 4686 4995 5922 6007 7078 3784 Size: 902 309 927 85 1071 4567 Fragments arranged by size: 4567 1071 927 902 309 85 BsmI GAATG_Cn' Cuts at: 4181 5372 5465 4181 Size: 1191 93 6577 Fragments arranged by size: 6577 1191 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2814 2814 Size: 7861 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3784 4686 4995 5180 5922 6007 7078 Size: 399 1994 902 309 185 742 85 1071 Cuts at: 7078 1391 Size: 2174 Fragments arranged by size: 2174 1994 1071 902 742 399 309 185 85 BspEI T'CCGG_A Cuts at: 7524 7524 Size: 7861 BspHI T'CATG_A Cuts at: 5750 7480 5750 Size: 1730 6131 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2335 3576 5216 844 Size: 1491 1241 1640 3489 Fragments arranged by size: 3489 1640 1491 1241 BsrBI CCG'CTC Cuts at: 1394 1823 5748 1394 Size: 429 3925 3507 Fragments arranged by size: 3925 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4992 6356 6530 65 Size: 1183 3744 1364 174 1396 Fragments arranged by size: 3744 1396 1364 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6505 6692 1700 Size: 110 4695 187 2869 Fragments arranged by size: 4695 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7861 BssHII G'CGCG_C Cuts at: 1925 2049 1925 Size: 124 7737 BssSI C'ACGA_G Cuts at: 3504 5921 6933 3504 Size: 2417 1012 4432 Fragments arranged by size: 4432 2417 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7861 BstBI TT'CG_AA Cuts at: 4146 6665 4146 Size: 2519 5342 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7861 BstYI r'GATC_y Cuts at: 1972 2233 2771 3760 5191 5558 5943 5960 7412 Size: 261 538 989 1431 367 385 17 1452 Cuts at: 7412 7498 7510 7856 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1431 989 538 385 367 346 261 86 17 12 BstZ17I GTA'TAC Cuts at: 3276 3276 Size: 7861 BtgI C'CryG_G Cuts at: 513 1065 2013 513 Size: 552 948 6361 Fragments arranged by size: 6361 948 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7122 Fragments arranged by size: 7122 683 56 BtsI GCAGTG_nn' Cuts at: 5377 6171 6191 5377 Size: 794 20 7047 Fragments arranged by size: 7047 794 20 ClaI AT'CG_AT Cuts at: 1554 4026 1554 Size: 2472 5389 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7826 DraI TTT'AAA Cuts at: 5165 5165 Size: 7861 DraIII CAC_nnn'GTG Cuts at: 4191 4191 Size: 7861 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6868 809 Size: 507 5552 1802 Fragments arranged by size: 5552 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2148 5308 Size: 54 1425 50 275 18 186 132 3160 Cuts at: 5308 6197 7826 8 Size: 889 1629 43 Fragments arranged by size: 3160 1629 1425 889 275 186 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 5308 1537 Size: 275 204 3292 4090 Fragments arranged by size: 4090 3292 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 2646 2661 3027 4263 4485 5791 1353 Size: 1101 192 15 366 1236 222 1306 3423 Fragments arranged by size: 3423 1306 1236 1101 366 222 192 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2016 6448 6822 6968 387 Size: 1064 138 427 4432 374 146 1280 Fragments arranged by size: 4432 1280 1064 427 374 146 138 EcoICRI GAG'CTC Cuts at: 4684 4993 4684 Size: 309 7552 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3008 3502 3749 4617 4670 4869 4979 Size: 393 1376 494 247 868 53 199 110 Cuts at: 4979 5924 6521 7308 7576 1239 Size: 945 597 787 268 1524 Fragments arranged by size: 1524 1376 945 868 787 597 494 393 268 247 199 110 53 EcoRI G'AATT_C Cuts at: 2744 2981 4734 5170 5548 2744 Size: 237 1753 436 378 5057 Fragments arranged by size: 5057 1753 436 378 237 EcoRV GAT'ATC Cuts at: 4024 4024 Size: 7861 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2422 2826 3779 3929 4472 4529 2422 Size: 404 953 150 543 57 5754 Fragments arranged by size: 5754 953 543 404 150 57 FspI TGC'GCA Cuts at: 6367 6367 Size: 7861 HaeII r_GCGC'y Cuts at: 1678 2157 2377 7008 1678 Size: 479 220 4631 2531 Fragments arranged by size: 4631 2531 479 220 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 2504 2536 2568 Size: 32 135 32 611 32 289 32 32 Cuts at: 2568 3032 3064 3498 3530 4112 4144 4693 4725 Size: 464 32 434 32 582 32 549 32 Cuts at: 4725 6502 6534 6576 6608 1373 Size: 1777 32 42 32 2626 Fragments arranged by size: 2626 1777 611 582 549 464 434 289 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5205 5451 669 Size: 988 281 3267 246 3079 Fragments arranged by size: 3267 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4511 748 Size: 3763 4098 HpaI GTT'AAC Cuts at: 5451 5451 Size: 7861 KasI G'GCGC_C Cuts at: 2373 2373 Size: 7861 KpnI G_GTAC'C Cuts at: 2762 4406 5186 2762 Size: 1644 780 5437 Fragments arranged by size: 5437 1644 780 MfeI C'AATT_G Cuts at: 4534 5460 4534 Size: 926 6935 MluI A'CGCG_T Cuts at: 3680 7737 3680 Size: 4057 3804 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2369 3570 6975 7159 1305 Size: 1064 1201 3405 184 2007 Fragments arranged by size: 3405 2007 1201 1064 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6039 Fragments arranged by size: 6039 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3353 5819 6178 6337 518 Size: 648 2187 2466 359 159 2042 Fragments arranged by size: 2466 2187 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2082 4658 4807 5277 5954 7102 7347 Size: 409 67 2576 149 470 677 1148 245 Cuts at: 7347 1606 Size: 2120 Fragments arranged by size: 2576 2120 1148 677 470 409 245 149 67 NarI GG'CG_CC Cuts at: 2374 2374 Size: 7861 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7309 NdeI CA'TA_TG Cuts at: 387 2700 3159 4819 387 Size: 2313 459 1660 3429 Fragments arranged by size: 3429 2313 1660 459 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7861 NotI GC'GGCC_GC Cuts at: 5308 5308 Size: 7861 NsiI A_TGCA'T Cuts at: 3435 3637 4243 3435 Size: 202 606 7053 Fragments arranged by size: 7053 606 202 NspI r_CATG'y Cuts at: 1132 1229 3565 3986 5219 6764 1132 Size: 97 2336 421 1233 1545 2229 Fragments arranged by size: 2336 2229 1545 1233 421 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7861 PciI A'CATG_T Cuts at: 3982 6760 3982 Size: 2778 5083 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7861 PfoI T'CCnGG_A Cuts at: 4686 4686 Size: 7861 PmeI GTTT'AAAC Cuts at: 5165 5165 Size: 7861 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5930 5962 7443 7475 5930 Size: 32 1481 32 6316 Fragments arranged by size: 6316 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7861 PsiI TTA'TAA Cuts at: 3827 4140 5431 3827 Size: 313 1291 6257 Fragments arranged by size: 6257 1291 313 PstI C_TGCA'G Cuts at: 830 3587 4063 5213 830 Size: 2757 476 1150 3478 Fragments arranged by size: 3478 2757 1150 476 PvuI CG_AT'CG Cuts at: 2001 2076 2001 Size: 75 7786 PvuII CAG'CTG Cuts at: 4658 4807 4658 Size: 149 7712 SacI G_AGCT'C Cuts at: 4686 4995 4686 Size: 309 7552 SacII CC_GC'GG Cuts at: 2016 2016 Size: 7861 SalI G'TCGA_C Cuts at: 1936 5203 1936 Size: 3267 4594 SapI GCTCTTCn'nnn_ Cuts at: 2454 4263 2454 Size: 1809 6052 SbfI CC_TGCA'GG Cuts at: 5213 5213 Size: 7861 ScaI AGT'ACT Cuts at: 3510 3581 6109 7821 3510 Size: 71 2528 1712 3550 Fragments arranged by size: 3550 2528 1712 71 SexAI A'CCwGG_T Cuts at: 3406 3406 Size: 7861 SfcI C'TryA_G Cuts at: 826 1045 3583 3840 4059 4159 5209 6344 7025 Size: 219 2538 257 219 100 1050 1135 681 Cuts at: 7025 7216 826 Size: 191 1471 Fragments arranged by size: 2538 1471 1135 1050 681 257 219 219 191 100 SfoI GGC'GCC Cuts at: 2375 2375 Size: 7861 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7861 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7861 SmaI CCC'GGG Cuts at: 1872 5188 1872 Size: 3316 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4970 5965 6866 7128 820 Size: 197 926 3027 995 901 262 1553 Fragments arranged by size: 3027 1553 995 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5652 493 Size: 5159 2702 SpeI A'CTAG_T Cuts at: 152 3142 5158 152 Size: 2990 2016 2855 Fragments arranged by size: 2990 2855 2016 SphI G_CATG'C Cuts at: 1132 5219 1132 Size: 4087 3774 SspI AAT'ATT Cuts at: 5 52 2187 4652 5785 5 Size: 47 2135 2465 1133 2081 Fragments arranged by size: 2465 2135 2081 1133 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7861 StyI C'CwwG_G Cuts at: 513 1065 2629 3127 4284 5300 513 Size: 552 1564 498 1157 1016 3074 Fragments arranged by size: 3074 1564 1157 1016 552 498 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3792 4297 5888 5905 6058 6243 6688 Size: 1255 2212 505 1591 17 153 185 445 Cuts at: 6688 325 Size: 1498 Fragments arranged by size: 2212 1591 1498 1255 505 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3508 3579 6107 Size: 274 80 33 51 1443 1531 71 2528 Cuts at: 6107 7819 96 Size: 1712 138 Fragments arranged by size: 2528 1712 1531 1443 274 138 80 71 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7821 7853 7821 Size: 32 7829 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7861 XbaI T'CTAG_A Cuts at: 5197 5197 Size: 7861 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4984 4984 Size: 7861 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7861 XmaI C'CCGG_G Cuts at: 1870 5186 1870 Size: 3316 4545 XmnI GAAnn'nnTTC Cuts at: 3954 4257 5990 3954 Size: 303 1733 5825 Fragments arranged by size: 5825 1733 303 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5669 276 Size: 53 83 186 936 4135 2468 Fragments arranged by size: 4135 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BaeI BsiWI BstXI Bsu36I EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SfiI SrfI SwaI