(Circular) (Six-base) MAPSORT of: pFN21AB1499.seq Check: 4956 from: 1 to: 8668 pFN21AB1499 8668 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5000 5000 Size: 8668 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6478 278 Size: 53 83 186 936 4942 2468 Fragments arranged by size: 4942 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3009 4667 6011 1937 Size: 1072 1658 1344 4594 Fragments arranged by size: 4594 1658 1344 1072 Acc65I G'GTAC_C Cuts at: 4423 4855 5727 5989 4423 Size: 432 872 262 7102 Fragments arranged by size: 7102 872 432 262 AclI AA'CG_TT Cuts at: 1563 6795 7168 1563 Size: 5232 373 3063 Fragments arranged by size: 5232 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4102 4728 6731 8115 8383 1632 Size: 2470 626 2003 1384 268 1917 Fragments arranged by size: 2470 2003 1917 1384 626 268 AfeI AGC'GCT Cuts at: 3053 3053 Size: 8668 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8471 AflIII A'CryG_T Cuts at: 1203 5069 5430 7567 8544 1203 Size: 3866 361 2137 977 1327 Fragments arranged by size: 3866 2137 1327 977 361 AgeI A'CCGG_T Cuts at: 7499 7499 Size: 8668 AhdI GACnn_n'nnGTC Cuts at: 7397 7397 Size: 8668 AleI CACnn'nnGTG Cuts at: 1166 3410 1166 Size: 2244 6424 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4898 1184 Size: 3714 4954 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4905 4937 4905 Size: 32 8636 AlwNI CAG_nnn'CTG Cuts at: 2053 2128 2458 3389 3548 5166 6411 7983 2053 Size: 75 330 931 159 1618 1245 1572 2738 Fragments arranged by size: 2738 1618 1572 1245 931 330 159 75 ApaI G_GGCC'C Cuts at: 2014 2804 2987 3159 3751 3782 2014 Size: 790 183 172 592 31 6900 Fragments arranged by size: 6900 790 592 183 172 31 ApaLI G'TGCA_C Cuts at: 2835 5024 6725 7881 2835 Size: 2189 1701 1156 3622 Fragments arranged by size: 3622 2189 1701 1156 ApoI r'AATT_y Cuts at: 1493 4441 4530 5959 5977 6203 6355 8464 1493 Size: 2948 89 1429 18 226 152 2109 1697 Fragments arranged by size: 2948 2109 1697 1429 226 152 89 18 AseI AT'TA_AT Cuts at: 160 1032 5409 7222 160 Size: 872 4377 1813 1606 Fragments arranged by size: 4377 1813 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8668 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2505 2769 2920 2992 4264 4936 Size: 73 271 291 264 151 72 1272 672 Cuts at: 4936 5993 1870 Size: 1057 4545 Fragments arranged by size: 4545 1272 1057 672 291 271 264 151 73 72 AvrII C'CTAG_G Cuts at: 3434 4771 3434 Size: 1337 7331 BamHI G'GATC_C Cuts at: 4544 5998 6365 4544 Size: 1454 367 6847 Fragments arranged by size: 6847 1454 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2358 2461 2855 4423 4855 Size: 325 217 627 571 103 394 1568 432 Cuts at: 4855 5084 5180 5687 5727 5989 7444 618 Size: 229 96 507 40 262 1455 1842 Fragments arranged by size: 1842 1568 1455 627 571 507 432 394 325 262 229 217 103 96 40 BanII G_rGCy'C Cuts at: 1391 2014 2115 2804 2987 3079 3159 3180 3623 Size: 623 101 689 183 92 80 21 443 Cuts at: 3623 3686 3696 3751 3782 4409 4586 4679 4937 Size: 63 10 55 31 627 177 93 258 Cuts at: 4937 5987 1391 Size: 1050 4072 Fragments arranged by size: 4072 1050 689 627 623 443 258 183 177 101 93 92 80 63 55 31 21 10 BbeI G_GCGC'C Cuts at: 2362 2362 Size: 8668 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2782 4276 4408 5048 928 Size: 971 883 1494 132 640 4548 Fragments arranged by size: 4548 1494 971 883 640 132 BbvCI CC'TCA_GC Cuts at: 2532 3290 2532 Size: 758 7910 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6859 6893 1903 Size: 34 4922 34 3678 Fragments arranged by size: 4922 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2667 4271 4594 6560 7770 2667 Size: 1604 323 1966 1210 3565 Fragments arranged by size: 3565 1966 1604 1210 323 BclI T'GATC_A Cuts at: 1548 5107 1548 Size: 3559 5109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2543 2864 Size: 107 122 71 631 605 337 534 321 Cuts at: 2864 2971 5364 5696 7279 136 Size: 107 2393 332 1583 1525 Fragments arranged by size: 2393 1583 1525 631 605 534 337 332 321 122 107 107 71 BglII A'GATC_T Cuts at: 3889 8663 3889 Size: 4774 3894 BlpI GC'TnA_GC Cuts at: 2560 3347 4161 6085 2560 Size: 787 814 1924 5143 Fragments arranged by size: 5143 1924 814 787 Bme1580I G_kGCm'C Cuts at: 1790 2014 2081 2466 2804 2839 2987 3159 3751 Size: 224 67 385 338 35 148 172 592 Cuts at: 3751 3782 5028 5639 5692 6729 7885 1790 Size: 31 1246 611 53 1037 1156 2573 Fragments arranged by size: 2573 1246 1156 1037 611 592 385 338 224 172 148 67 53 35 31 BmgBI CAC'GTC Cuts at: 1318 3090 1318 Size: 1772 6896 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2662 3155 3342 Size: 416 53 83 399 24 1245 493 187 Cuts at: 3342 3777 4137 4509 4682 5950 6399 7357 442 Size: 435 360 372 173 1268 449 958 1753 Fragments arranged by size: 1753 1268 1245 958 493 449 435 416 399 372 360 187 173 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8668 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4846 5044 5692 4846 Size: 198 648 7822 Fragments arranged by size: 7822 648 198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3194 3206 3678 3779 4153 4159 4431 4652 Size: 1955 12 472 101 374 6 272 221 Cuts at: 4652 4855 4937 7328 1239 Size: 203 82 2391 2579 Fragments arranged by size: 2579 2391 1955 472 374 272 221 203 101 82 12 6 Bpu10I CC'TnA_GC Cuts at: 2532 3290 4146 2532 Size: 758 856 7054 Fragments arranged by size: 7054 856 758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4600 4666 6793 7658 7956 4600 Size: 66 2127 865 298 5312 Fragments arranged by size: 5312 2127 865 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3096 3104 4019 4127 4715 7331 882 Size: 617 1597 8 915 108 588 2616 2219 Fragments arranged by size: 2616 2219 1597 915 617 588 108 8 BsaAI yAC'GTr Cuts at: 493 1204 2797 5433 6459 493 Size: 711 1593 2636 1026 2702 Fragments arranged by size: 2702 2636 1593 1026 711 BsaBI GATnn'nnATC Cuts at: 8662 8662 Size: 8668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2359 2963 3060 4616 Size: 53 83 186 936 826 604 97 1556 Cuts at: 4616 6475 6857 7492 275 Size: 1859 382 635 1451 Fragments arranged by size: 1859 1556 1451 936 826 635 604 382 186 97 83 53 BsaWI w'CCGG_w Cuts at: 3024 3490 5319 5389 7101 7499 7773 7920 8331 Size: 466 1829 70 1712 398 274 147 411 Cuts at: 8331 3024 Size: 3361 Fragments arranged by size: 3361 1829 1712 466 411 398 274 147 70 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3128 3158 3557 3587 4157 Size: 30 165 30 1725 30 399 30 570 Cuts at: 4157 4187 4731 4761 4924 4954 1178 Size: 30 544 30 163 30 4892 Fragments arranged by size: 4892 1725 570 544 399 165 163 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3203 3278 4669 4973 4991 5033 1186 Size: 2017 75 1391 304 18 42 4821 Fragments arranged by size: 4821 2017 1391 304 75 42 18 BseYI C'CCAG_C Cuts at: 2066 2128 2776 2986 3362 3528 3725 3845 5225 Size: 62 648 210 376 166 197 120 1380 Cuts at: 5225 5751 7871 8367 2066 Size: 526 2120 496 2367 Fragments arranged by size: 2367 2120 1380 648 526 496 376 210 197 166 120 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 2867 4358 5800 1163 Size: 573 429 702 1491 1442 4031 Fragments arranged by size: 4031 1491 1442 702 573 429 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2695 6118 6879 7907 1540 Size: 275 186 694 3423 761 1028 2301 Fragments arranged by size: 3423 2301 1028 761 694 275 186 BsiHKAI G_wGCw'C Cuts at: 2839 3079 3686 4167 5028 5646 6729 6814 7885 Size: 240 607 481 861 618 1083 85 1071 Cuts at: 7885 2839 Size: 3622 Fragments arranged by size: 3622 1083 1071 861 618 607 481 240 85 BsmI GAATG_Cn' Cuts at: 3866 5011 5677 6179 6272 3866 Size: 1145 666 502 93 6262 Fragments arranged by size: 6262 1145 666 502 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2558 2558 Size: 8668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2081 2115 2466 2804 2839 2987 Size: 399 224 67 34 351 338 35 148 Cuts at: 2987 3079 3159 3180 3623 3686 3696 3751 3782 Size: 92 80 21 443 63 10 55 31 Cuts at: 3782 4167 4409 4586 4679 4937 5028 5639 5646 Size: 385 242 177 93 258 91 611 7 Cuts at: 5646 5692 5987 6729 6814 7885 1391 Size: 46 295 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 611 443 399 385 351 338 295 258 242 224 177 148 93 92 91 85 80 67 63 55 46 35 34 31 21 10 7 BspEI T'CCGG_A Cuts at: 8331 8331 Size: 8668 BspHI T'CATG_A Cuts at: 6557 8287 6557 Size: 1730 6938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5000 6023 844 Size: 4156 1023 3489 Fragments arranged by size: 4156 3489 1023 BsrBI CCG'CTC Cuts at: 1394 1823 5361 6555 1394 Size: 429 3538 1194 3507 Fragments arranged by size: 3538 3507 1194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2568 5743 5791 7163 7337 65 Size: 1183 1320 3175 48 1372 174 1396 Fragments arranged by size: 3175 1396 1372 1320 1183 174 48 BsrFI r'CCGG_y Cuts at: 1700 1810 2825 2970 3029 4507 4780 7312 7499 Size: 110 1015 145 59 1478 273 2532 187 Cuts at: 7499 1700 Size: 2869 Fragments arranged by size: 2869 2532 1478 1015 273 187 145 110 59 BsrGI T'GTAC_A Cuts at: 96 4359 96 Size: 4263 4405 BssHII G'CGCG_C Cuts at: 1925 2829 1925 Size: 904 7764 BssSI C'ACGA_G Cuts at: 6728 7740 6728 Size: 1012 7656 BstAPI GCAn_nnn'nTGC Cuts at: 794 2053 3389 794 Size: 1259 1336 6073 Fragments arranged by size: 6073 1336 1259 BstBI TT'CG_AA Cuts at: 4911 7472 4911 Size: 2561 6107 BstEII G'GTnAC_C Cuts at: 1184 3202 1184 Size: 2018 6650 BstXI CCAn_nnnn'nTGG Cuts at: 2287 3646 3696 2287 Size: 1359 50 7259 Fragments arranged by size: 7259 1359 50 BstYI r'GATC_y Cuts at: 1972 3889 4544 5032 5998 6365 6750 6767 8219 Size: 1917 655 488 966 367 385 17 1452 Cuts at: 8219 8305 8317 8663 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1917 1452 966 655 488 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4253 4587 4840 4253 Size: 334 253 8081 Fragments arranged by size: 8081 334 253 BtgI C'CryG_G Cuts at: 513 1065 2004 2198 3579 3714 3999 4181 4488 Size: 552 939 194 1381 135 285 182 307 Cuts at: 4488 4859 5272 513 Size: 371 413 3909 Fragments arranged by size: 3909 1381 939 552 413 371 307 285 194 182 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7929 Fragments arranged by size: 7929 683 56 BtsI GCAGTG_nn' Cuts at: 2339 3387 3558 3610 5670 6184 6978 6998 2339 Size: 1048 171 52 2060 514 794 20 4009 Fragments arranged by size: 4009 2060 1048 794 514 171 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8633 DraI TTT'AAA Cuts at: 5972 5972 Size: 8668 DraIII CAC_nnn'GTG Cuts at: 3986 5761 3986 Size: 1775 6893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7675 809 Size: 507 6359 1802 Fragments arranged by size: 6359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2201 2289 2313 Size: 54 1425 50 275 18 371 88 24 Cuts at: 2313 2823 2931 3576 3607 3876 3903 5365 5763 Size: 510 108 645 31 269 27 1462 398 Cuts at: 5763 6115 7004 8633 8 Size: 352 889 1629 43 Fragments arranged by size: 1629 1462 1425 889 645 510 398 371 352 275 269 108 88 54 50 43 31 27 24 18 EagI C'GGCC_G Cuts at: 1537 1812 6115 1537 Size: 275 4303 4090 Fragments arranged by size: 4303 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3189 6598 1353 Size: 1836 3409 3423 Fragments arranged by size: 3423 3409 1836 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 7255 7629 7775 387 Size: 1064 138 585 5081 374 146 1280 Fragments arranged by size: 5081 1280 1064 585 374 146 138 EcoICRI GAG'CTC Cuts at: 3077 3684 3077 Size: 607 8061 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3194 3206 3678 3779 4102 4153 4159 Size: 393 1562 12 472 101 323 51 6 Cuts at: 4159 4431 4652 4728 4855 4937 6731 7328 8115 Size: 272 221 76 127 82 1794 597 787 Cuts at: 8115 8383 1239 Size: 268 1524 Fragments arranged by size: 1794 1562 1524 787 597 472 393 323 272 268 221 127 101 82 76 51 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 3188 4634 3188 Size: 1446 7222 EcoO109I rG'GnC_Cy Cuts at: 2010 2011 2455 2902 2983 3155 3156 3307 3747 Size: 1 444 447 81 172 1 151 440 Cuts at: 3747 3748 3778 3779 4342 4472 4864 4921 4995 Size: 1 30 1 563 130 392 57 74 Cuts at: 4995 5802 2010 Size: 807 4876 Fragments arranged by size: 4876 807 563 447 444 440 392 172 151 130 81 74 57 30 1 1 1 1 EcoRI G'AATT_C Cuts at: 5977 6355 5977 Size: 378 8290 EcoRV GAT'ATC Cuts at: 2089 2089 Size: 8668 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4323 4323 Size: 8668 FspI TGC'GCA Cuts at: 3002 7174 3002 Size: 4172 4496 FspAI rTGC'GCAy Cuts at: 3002 3002 Size: 8668 HaeII r_GCGC'y Cuts at: 1678 2362 2977 3055 3451 3472 3528 5587 7815 Size: 684 615 78 396 21 56 2059 2228 Cuts at: 7815 1678 Size: 2531 Fragments arranged by size: 2531 2228 2059 684 615 396 78 56 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3101 3128 3133 3160 7309 Size: 32 135 32 1529 27 5 27 4149 Cuts at: 7309 7341 7383 7415 1373 Size: 32 42 32 2626 Fragments arranged by size: 4149 2626 1529 135 42 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 4153 6012 6258 669 Size: 988 281 2215 1859 246 3079 Fragments arranged by size: 3079 2215 1859 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8668 HpaI GTT'AAC Cuts at: 6258 6258 Size: 8668 KasI G'GCGC_C Cuts at: 2358 2358 Size: 8668 KpnI G_GTAC'C Cuts at: 4427 4859 5731 5993 4427 Size: 432 872 262 7102 Fragments arranged by size: 7102 872 432 262 MfeI C'AATT_G Cuts at: 6267 6267 Size: 8668 MluI A'CGCG_T Cuts at: 8544 8544 Size: 8668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4630 7782 7966 1305 Size: 3325 3152 184 2007 Fragments arranged by size: 3325 3152 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2291 2315 3578 3609 3878 Size: 54 1425 343 459 24 1263 31 269 Cuts at: 3878 3905 5765 10 Size: 27 1860 2913 Fragments arranged by size: 2913 1860 1425 1263 459 343 269 54 31 27 24 MslI CAynn'nnrTG Cuts at: 518 1166 2285 3410 3593 6626 6985 7144 518 Size: 648 1119 1125 183 3033 359 159 2042 Fragments arranged by size: 3033 2042 1125 1119 648 359 183 159 MspA1I CmG'CkG Cuts at: 1606 2421 2881 5274 6084 6761 7909 8154 1606 Size: 815 460 2393 810 677 1148 245 2120 Fragments arranged by size: 2393 2120 1148 815 810 677 460 245 NaeI GCC'GGC Cuts at: 2827 2972 4509 2827 Size: 145 1537 6986 Fragments arranged by size: 6986 1537 145 NarI GG'CG_CC Cuts at: 2359 2359 Size: 8668 NcoI C'CATG_G Cuts at: 513 1065 2004 3579 3714 4181 4488 513 Size: 552 939 1575 135 467 307 4693 Fragments arranged by size: 4693 1575 939 552 467 307 135 NdeI CA'TA_TG Cuts at: 387 387 Size: 8668 NgoMIV G'CCGG_C Cuts at: 2825 2970 4507 2825 Size: 145 1537 6986 Fragments arranged by size: 6986 1537 145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8668 NotI GC'GGCC_GC Cuts at: 6115 6115 Size: 8668 NspI r_CATG'y Cuts at: 1132 1229 2122 5073 6026 7571 1132 Size: 97 893 2951 953 1545 2229 Fragments arranged by size: 2951 2229 1545 953 893 97 PasI CC'CwG_GG Cuts at: 1348 3302 3624 3858 4107 4399 4726 4894 1348 Size: 1954 322 234 249 292 327 168 5122 Fragments arranged by size: 5122 1954 327 322 292 249 234 168 PciI A'CATG_T Cuts at: 5069 7567 5069 Size: 2498 6170 PflMI CCAn_nnn'nTGG Cuts at: 1108 2322 3122 3927 4182 1108 Size: 1214 800 805 255 5594 Fragments arranged by size: 5594 1214 805 800 255 PfoI T'CCnGG_A Cuts at: 3216 3756 3216 Size: 540 8128 PmeI GTTT'AAAC Cuts at: 5972 5972 Size: 8668 PmlI CAC'GTG Cuts at: 5433 5433 Size: 8668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6737 6769 8250 8282 6737 Size: 32 1481 32 7123 Fragments arranged by size: 7123 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2455 3307 4342 4472 4864 4921 4995 2455 Size: 852 1035 130 392 57 74 6128 Fragments arranged by size: 6128 1035 852 392 130 74 57 PshAI GACnn'nnGTC Cuts at: 1613 2335 5378 1613 Size: 722 3043 4903 Fragments arranged by size: 4903 3043 722 PsiI TTA'TAA Cuts at: 6238 6238 Size: 8668 PspOMI G'GGCC_C Cuts at: 2010 2800 2983 3155 3747 3778 2010 Size: 790 183 172 592 31 6900 Fragments arranged by size: 6900 790 592 183 172 31 PstI C_TGCA'G Cuts at: 830 2049 2196 3394 3553 4024 4810 5162 6020 Size: 1219 147 1198 159 471 786 352 858 Cuts at: 6020 830 Size: 3478 Fragments arranged by size: 3478 1219 1198 858 786 471 352 159 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8668 PvuII CAG'CTG Cuts at: 2881 2881 Size: 8668 RsrII CG'GwC_CG Cuts at: 3027 3027 Size: 8668 SacI G_AGCT'C Cuts at: 3079 3686 3079 Size: 607 8061 SacII CC_GC'GG Cuts at: 5275 5275 Size: 8668 SalI G'TCGA_C Cuts at: 1936 6010 1936 Size: 4074 4594 SanDI GG'GwC_CC Cuts at: 3307 4864 4995 3307 Size: 1557 131 6980 Fragments arranged by size: 6980 1557 131 SbfI CC_TGCA'GG Cuts at: 6020 6020 Size: 8668 ScaI AGT'ACT Cuts at: 6916 8628 6916 Size: 1712 6956 SexAI A'CCwGG_T Cuts at: 2457 2457 Size: 8668 SfcI C'TryA_G Cuts at: 826 1045 2045 2192 2588 3390 3549 4020 4209 Size: 219 1000 147 396 802 159 471 189 Cuts at: 4209 4326 4461 4806 5098 5158 6016 7151 7832 Size: 117 135 345 292 60 858 1135 681 Cuts at: 7832 8023 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1000 858 802 681 471 396 345 292 219 191 189 159 147 135 117 60 SfiI GGCCn_nnn'nGGCC Cuts at: 2543 2543 Size: 8668 SfoI GGC'GCC Cuts at: 2360 2360 Size: 8668 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8668 SgrAI Cr'CCGG_yG Cuts at: 1700 2970 1700 Size: 1270 7398 SmaI CCC'GGG Cuts at: 1872 2507 2771 2922 2994 4266 4938 5995 1872 Size: 635 264 151 72 1272 672 1057 4545 Fragments arranged by size: 4545 1272 1057 672 635 264 151 72 SmlI C'TyrA_G Cuts at: 820 1017 1943 4579 4681 6772 7673 7935 820 Size: 197 926 2636 102 2091 901 262 1553 Fragments arranged by size: 2636 2091 1553 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 6459 493 Size: 5966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8668 SphI G_CATG'C Cuts at: 1132 6026 1132 Size: 4894 3774 SspI AAT'ATT Cuts at: 5 52 6592 5 Size: 47 6540 2081 Fragments arranged by size: 6540 2081 47 StuI AGG'CCT Cuts at: 1517 2546 1517 Size: 1029 7639 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2241 2292 2466 3434 3579 Size: 552 939 24 213 51 174 968 145 Cuts at: 3579 3714 3822 4029 4181 4451 4488 4771 6107 Size: 135 108 207 152 270 37 283 1336 Cuts at: 6107 513 Size: 3074 Fragments arranged by size: 3074 1336 968 939 552 283 270 213 207 174 152 145 135 108 51 37 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 3739 4713 5943 6695 6712 6865 Size: 1255 1101 1058 974 1230 752 17 153 Cuts at: 6865 7050 7495 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1230 1101 1058 974 752 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4359 5703 6914 Size: 274 80 33 51 1443 2382 1344 1211 Cuts at: 6914 8626 96 Size: 1712 138 Fragments arranged by size: 2382 1712 1443 1344 1211 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4157 4189 4464 4468 4496 4500 4536 4568 4731 Size: 32 275 4 28 4 36 32 163 Cuts at: 4731 4763 4924 4956 5879 5911 8628 8660 4157 Size: 32 161 32 923 32 2717 32 4165 Fragments arranged by size: 4165 2717 923 275 163 161 36 32 32 32 32 32 32 28 4 4 Tth111I GACn'n_nGTC Cuts at: 1316 2254 2300 1316 Size: 938 46 7684 Fragments arranged by size: 7684 938 46 XbaI T'CTAG_A Cuts at: 6004 6004 Size: 8668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 2675 3810 4669 5937 2380 Size: 295 1135 859 1268 5111 Fragments arranged by size: 5111 1268 1135 859 295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8668 XmaI C'CCGG_G Cuts at: 1870 2505 2769 2920 2992 4264 4936 5993 1870 Size: 635 264 151 72 1272 672 1057 4545 Fragments arranged by size: 4545 1272 1057 672 635 264 151 72 XmnI GAAnn'nnTTC Cuts at: 6797 6797 Size: 8668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6476 276 Size: 53 83 186 936 4942 2468 Fragments arranged by size: 4942 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I FseI NruI NsiI PacI PspXI PsrI SapI SrfI SwaI