(Circular) (Six-base) MAPSORT of: pFN21AB1393.seq Check: 2628 from: 1 to: 7135 pFN21AB1393 7135 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2134 2134 Size: 7135 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4945 278 Size: 53 83 186 936 3409 2468 Fragments arranged by size: 3409 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4359 4478 1937 Size: 2422 119 4594 Fragments arranged by size: 4594 2422 119 Acc65I G'GTAC_C Cuts at: 4456 4456 Size: 7135 AclI AA'CG_TT Cuts at: 1563 5262 5635 1563 Size: 3699 373 3063 Fragments arranged by size: 3699 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 2262 2331 2345 2508 2841 3927 5198 Size: 434 196 69 14 163 333 1086 1271 Cuts at: 5198 6582 6850 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1271 1086 434 333 268 196 163 69 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6938 AflIII A'CryG_T Cuts at: 1203 6034 7011 1203 Size: 4831 977 1327 Fragments arranged by size: 4831 1327 977 AgeI A'CCGG_T Cuts at: 5966 5966 Size: 7135 AhdI GACnn_n'nnGTC Cuts at: 5864 5864 Size: 7135 AleI CACnn'nnGTG Cuts at: 1166 2070 3212 1166 Size: 904 1142 5089 Fragments arranged by size: 5089 1142 904 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7135 AlwNI CAG_nnn'CTG Cuts at: 2263 4878 6450 2263 Size: 2615 1572 2948 Fragments arranged by size: 2948 2615 1572 ApaLI G'TGCA_C Cuts at: 5192 6348 5192 Size: 1156 5979 ApoI r'AATT_y Cuts at: 1493 2607 3346 4444 4670 4822 6931 1493 Size: 1114 739 1098 226 152 2109 1697 Fragments arranged by size: 2109 1697 1114 1098 739 226 152 AseI AT'TA_AT Cuts at: 160 1032 5689 160 Size: 872 4657 1606 Fragments arranged by size: 4657 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 AvaI C'yCGr_G Cuts at: 1870 1943 4460 1870 Size: 73 2517 4545 Fragments arranged by size: 4545 2517 73 BamHI G'GATC_C Cuts at: 4465 4832 4465 Size: 367 6768 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2412 4128 4456 5911 618 Size: 325 217 627 625 1716 328 1455 1842 Fragments arranged by size: 1842 1716 1455 627 625 328 325 217 BanII G_rGCy'C Cuts at: 1391 2409 2914 3396 4329 4454 1391 Size: 1018 505 482 933 125 4072 Fragments arranged by size: 4072 1018 933 505 482 125 BbeI G_GCGC'C Cuts at: 4132 4132 Size: 7135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2946 928 Size: 971 1047 5117 Fragments arranged by size: 5117 1047 971 BbvCI CC'TCA_GC Cuts at: 3140 4379 3140 Size: 1239 5896 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5326 5360 1903 Size: 34 3389 34 3678 Fragments arranged by size: 3678 3389 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5027 6237 5027 Size: 1210 5925 BclI T'GATC_A Cuts at: 1548 1548 Size: 7135 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2483 5746 136 Size: 107 122 71 631 605 811 3263 1525 Fragments arranged by size: 3263 1525 811 631 605 122 107 71 BglII A'GATC_T Cuts at: 7130 7130 Size: 7135 BlpI GC'TnA_GC Cuts at: 2267 4552 2267 Size: 2285 4850 Bme1580I G_kGCm'C Cuts at: 1790 3172 3248 5196 6352 1790 Size: 1382 76 1948 1156 2573 Fragments arranged by size: 2573 1948 1382 1156 76 BmgBI CAC'GTC Cuts at: 1318 2111 1318 Size: 793 6342 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2987 3469 3736 Size: 416 53 83 399 24 1570 482 267 Cuts at: 3736 4083 4323 4866 5824 442 Size: 347 240 543 958 1753 Fragments arranged by size: 1753 1570 958 543 482 416 399 347 267 240 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7135 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3410 3410 Size: 7135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3527 4079 5795 1239 Size: 2288 552 1716 2579 Fragments arranged by size: 2579 2288 1716 552 Bpu10I CC'TnA_GC Cuts at: 2408 3140 4379 2408 Size: 732 1239 5164 Fragments arranged by size: 5164 1239 732 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2303 2315 2718 2965 5260 6125 6423 2303 Size: 12 403 247 2295 865 298 3015 Fragments arranged by size: 3015 2295 865 403 298 247 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2187 2279 2592 5798 882 Size: 617 688 92 313 3206 2219 Fragments arranged by size: 3206 2219 688 617 313 92 BsaAI yAC'GTr Cuts at: 493 1204 3871 4926 493 Size: 711 2667 1055 2702 Fragments arranged by size: 2702 2667 1055 711 BsaBI GATnn'nnATC Cuts at: 7129 7129 Size: 7135 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3120 3456 4129 4942 Size: 53 83 186 936 1587 336 673 813 Cuts at: 4942 5324 5959 275 Size: 382 635 1451 Fragments arranged by size: 1587 1451 936 813 673 635 382 336 186 83 53 BsaWI w'CCGG_w Cuts at: 2703 2776 4165 5568 5966 6240 6387 6798 2703 Size: 73 1389 1403 398 274 147 411 3040 Fragments arranged by size: 3040 1403 1389 411 398 274 147 73 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3151 3181 3668 3698 1178 Size: 30 165 30 1748 30 487 30 4615 Fragments arranged by size: 4615 1748 487 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2384 2405 2690 3087 3258 3692 4112 1186 Size: 1198 21 285 397 171 434 420 4209 Fragments arranged by size: 4209 1198 434 420 397 285 171 21 BseYI C'CCAG_C Cuts at: 2496 3198 3364 6338 6834 2496 Size: 702 166 2974 496 2797 Fragments arranged by size: 2974 2797 702 496 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 3672 3890 1163 Size: 573 792 1144 218 4408 Fragments arranged by size: 4408 1144 792 573 218 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4585 5346 6374 1540 Size: 275 186 2584 761 1028 2301 Fragments arranged by size: 2584 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 3033 3485 5196 5281 6352 2409 Size: 624 452 1711 85 1071 3192 Fragments arranged by size: 3192 1711 1071 624 452 85 BsmI GAATG_Cn' Cuts at: 3789 4646 4739 3789 Size: 857 93 6185 Fragments arranged by size: 6185 857 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2409 2914 3033 3172 3248 3396 3485 Size: 399 619 505 119 139 76 148 89 Cuts at: 3485 4329 4454 5196 5281 6352 1391 Size: 844 125 742 85 1071 2174 Fragments arranged by size: 2174 1071 844 742 619 505 399 148 139 125 119 89 85 76 BspEI T'CCGG_A Cuts at: 4165 6798 4165 Size: 2633 4502 BspHI T'CATG_A Cuts at: 3760 5024 6754 3760 Size: 1264 1730 4141 Fragments arranged by size: 4141 1730 1264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2134 4490 844 Size: 1290 2356 3489 Fragments arranged by size: 3489 2356 1290 BsrBI CCG'CTC Cuts at: 1394 1823 5022 1394 Size: 429 3199 3507 Fragments arranged by size: 3507 3199 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2882 5630 5804 65 Size: 1183 1634 2748 174 1396 Fragments arranged by size: 2748 1634 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3909 5779 5966 1700 Size: 110 2099 1870 187 2869 Fragments arranged by size: 2869 2099 1870 187 110 BsrGI T'GTAC_A Cuts at: 96 3952 96 Size: 3856 3279 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7135 BssSI C'ACGA_G Cuts at: 2038 5195 6207 2038 Size: 3157 1012 2966 Fragments arranged by size: 3157 2966 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4010 794 Size: 3216 3919 BstBI TT'CG_AA Cuts at: 5939 5939 Size: 7135 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7135 BstYI r'GATC_y Cuts at: 1972 4465 4832 5217 5234 6686 6772 6784 7130 Size: 2493 367 385 17 1452 86 12 346 Cuts at: 7130 1972 Size: 1977 Fragments arranged by size: 2493 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3588 4124 3588 Size: 536 6599 BtgI C'CryG_G Cuts at: 513 1065 2004 3123 4039 513 Size: 552 939 1119 916 3609 Fragments arranged by size: 3609 1119 939 916 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6396 Fragments arranged by size: 6396 683 56 BtsI GCAGTG_nn' Cuts at: 3207 3554 3657 3689 3898 4651 5445 5465 3207 Size: 347 103 32 209 753 794 20 4877 Fragments arranged by size: 4877 794 753 347 209 103 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7135 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2865 2900 529 Size: 35 2301 35 4764 Fragments arranged by size: 4764 2301 35 35 DraI TTT'AAA Cuts at: 2833 4439 2833 Size: 1606 5529 DraIII CAC_nnn'GTG Cuts at: 2224 3973 2224 Size: 1749 5386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2314 6142 809 Size: 507 998 3828 1802 Fragments arranged by size: 3828 1802 998 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3154 3562 3914 Size: 54 1425 50 275 18 1324 408 352 Cuts at: 3914 4241 4582 5471 7100 8 Size: 327 341 889 1629 43 Fragments arranged by size: 1629 1425 1324 889 408 352 341 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4582 1537 Size: 275 2770 4090 Fragments arranged by size: 4090 2770 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2238 2427 3821 5065 1353 Size: 689 196 189 1394 1244 3423 Fragments arranged by size: 3423 1394 1244 689 196 189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3041 5722 6096 6242 387 Size: 1064 138 1452 2681 374 146 1280 Fragments arranged by size: 2681 1452 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2407 2407 Size: 7135 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2262 2331 2345 2508 2841 3527 Size: 393 434 196 69 14 163 333 686 Cuts at: 3527 3927 4079 5198 5795 6582 6850 1239 Size: 400 152 1119 597 787 268 1524 Fragments arranged by size: 1524 1119 787 686 597 434 400 393 333 268 196 163 152 69 14 EcoO109I rG'GnC_Cy Cuts at: 2052 2738 2815 3257 4375 2052 Size: 686 77 442 1118 4812 Fragments arranged by size: 4812 1118 686 442 77 EcoRI G'AATT_C Cuts at: 4444 4822 4444 Size: 378 6757 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2267 2267 Size: 7135 FspI TGC'GCA Cuts at: 5641 5641 Size: 7135 HaeII r_GCGC'y Cuts at: 1678 4132 6282 1678 Size: 2454 2150 2531 Fragments arranged by size: 2531 2454 2150 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2554 2586 2716 Size: 32 135 32 542 32 408 32 130 Cuts at: 2716 2748 3133 3165 3215 3247 3668 3700 5776 Size: 32 385 32 50 32 421 32 2076 Cuts at: 5776 5808 5850 5882 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2076 542 421 408 385 135 130 50 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3775 3883 4479 4725 669 Size: 988 281 1837 108 596 246 3079 Fragments arranged by size: 3079 1837 988 596 281 246 108 HindIII A'AGCT_T Cuts at: 748 3056 3638 748 Size: 2308 582 4245 Fragments arranged by size: 4245 2308 582 HpaI GTT'AAC Cuts at: 4725 4725 Size: 7135 KasI G'GCGC_C Cuts at: 4128 4128 Size: 7135 KpnI G_GTAC'C Cuts at: 4460 4460 Size: 7135 MfeI C'AATT_G Cuts at: 4734 4734 Size: 7135 MluI A'CGCG_T Cuts at: 7011 7011 Size: 7135 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2418 2648 3987 6249 6433 1305 Size: 1113 230 1339 2262 184 2007 Fragments arranged by size: 2262 2007 1339 1113 230 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3564 10 Size: 54 1425 343 1732 3581 Fragments arranged by size: 3581 1732 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2070 3212 3707 3869 5093 5452 5611 Size: 648 904 1142 495 162 1224 359 159 Cuts at: 5611 518 Size: 2042 Fragments arranged by size: 2042 1224 1142 904 648 495 359 162 159 MspA1I CmG'CkG Cuts at: 1606 2500 3920 4551 5228 6376 6621 1606 Size: 894 1420 631 677 1148 245 2120 Fragments arranged by size: 2120 1420 1148 894 677 631 245 NarI GG'CG_CC Cuts at: 4129 4129 Size: 7135 NcoI C'CATG_G Cuts at: 513 1065 2004 4039 513 Size: 552 939 2035 3609 Fragments arranged by size: 3609 2035 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7135 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7135 NotI GC'GGCC_GC Cuts at: 4582 4582 Size: 7135 NspI r_CATG'y Cuts at: 1132 1229 3881 4493 6038 1132 Size: 97 2652 612 1545 2229 Fragments arranged by size: 2652 2229 1545 612 97 PasI CC'CwG_GG Cuts at: 1348 2176 1348 Size: 828 6307 PciI A'CATG_T Cuts at: 6034 6034 Size: 7135 PflMI CCAn_nnn'nTGG Cuts at: 1108 3549 1108 Size: 2441 4694 PfoI T'CCnGG_A Cuts at: 2558 4056 2558 Size: 1498 5637 PmeI GTTT'AAAC Cuts at: 4439 4439 Size: 7135 PmlI CAC'GTG Cuts at: 3871 3871 Size: 7135 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5204 5236 6717 6749 5204 Size: 32 1481 32 5590 Fragments arranged by size: 5590 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2052 2815 3257 2052 Size: 763 442 5930 Fragments arranged by size: 5930 763 442 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7135 PsiI TTA'TAA Cuts at: 4705 4705 Size: 7135 PstI C_TGCA'G Cuts at: 830 4487 830 Size: 3657 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7135 PvuII CAG'CTG Cuts at: 2500 3920 2500 Size: 1420 5715 SacI G_AGCT'C Cuts at: 2409 2409 Size: 7135 SalI G'TCGA_C Cuts at: 1936 4477 1936 Size: 2541 4594 SbfI CC_TGCA'GG Cuts at: 4487 4487 Size: 7135 ScaI AGT'ACT Cuts at: 5383 7095 5383 Size: 1712 5423 SexAI A'CCwGG_T Cuts at: 2220 4416 2220 Size: 2196 4939 SfcI C'TryA_G Cuts at: 826 1045 2865 4360 4483 5618 6299 6490 826 Size: 219 1820 1495 123 1135 681 191 1471 Fragments arranged by size: 1820 1495 1471 1135 681 219 191 123 SfoI GGC'GCC Cuts at: 4130 4130 Size: 7135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 SgrAI Cr'CCGG_yG Cuts at: 1700 3909 1700 Size: 2209 4926 SmaI CCC'GGG Cuts at: 1872 4462 1872 Size: 2590 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 2330 2733 2944 5239 6140 Size: 197 926 339 48 403 211 2295 901 Cuts at: 6140 6402 820 Size: 262 1553 Fragments arranged by size: 2295 1553 926 901 403 339 262 211 197 48 SnaBI TAC'GTA Cuts at: 493 4926 493 Size: 4433 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7135 SphI G_CATG'C Cuts at: 1132 4493 1132 Size: 3361 3774 SspI AAT'ATT Cuts at: 5 52 5059 5 Size: 47 5007 2081 Fragments arranged by size: 5007 2081 47 StuI AGG'CCT Cuts at: 1517 4047 1517 Size: 2530 4605 StyI C'CwwG_G Cuts at: 513 1065 2004 3006 3438 4039 4244 4574 513 Size: 552 939 1002 432 601 205 330 3074 Fragments arranged by size: 3074 1002 939 601 552 432 330 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3636 3697 4030 5162 5179 5332 5517 Size: 1255 2056 61 333 1132 17 153 185 Cuts at: 5517 5962 325 Size: 445 1498 Fragments arranged by size: 2056 1498 1255 1132 445 333 185 153 61 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3744 3952 5381 Size: 274 80 33 51 1443 1767 208 1429 Cuts at: 5381 7093 96 Size: 1712 138 Fragments arranged by size: 1767 1712 1443 1429 274 208 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2208 2240 3151 3183 7095 7127 2208 Size: 32 911 32 3912 32 2216 Fragments arranged by size: 3912 2216 911 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4337 1316 Size: 3021 4114 XbaI T'CTAG_A Cuts at: 2113 2935 3767 4471 2113 Size: 822 832 704 4777 Fragments arranged by size: 4777 832 822 704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7135 XmaI C'CCGG_G Cuts at: 1870 4460 1870 Size: 2590 4545 XmnI GAAnn'nnTTC Cuts at: 2187 5264 2187 Size: 3077 4058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4943 276 Size: 53 83 186 936 3409 2468 Fragments arranged by size: 3409 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI