(Circular) (Six-base) MAPSORT of: pFN21AB1326.seq Check: 8273 from: 1 to: 7531 pFN21AB1326 7531 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3436 4393 4657 3436 Size: 957 264 6310 Fragments arranged by size: 6310 957 264 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2277 5341 278 Size: 53 83 186 936 741 3064 2468 Fragments arranged by size: 3064 2468 936 741 186 83 53 AccI GT'mk_AC Cuts at: 1937 4874 1937 Size: 2937 4594 Acc65I G'GTAC_C Cuts at: 3987 4852 3987 Size: 865 6666 AclI AA'CG_TT Cuts at: 1563 5658 6031 1563 Size: 4095 373 3063 Fragments arranged by size: 4095 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2755 2924 3221 3701 5594 6978 7246 Size: 695 428 169 297 480 1893 1384 268 Cuts at: 7246 1632 Size: 1917 Fragments arranged by size: 1917 1893 1384 695 480 428 297 268 169 AfeI AGC'GCT Cuts at: 2845 2845 Size: 7531 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7334 AflIII A'CryG_T Cuts at: 1203 3240 6430 7407 1203 Size: 2037 3190 977 1327 Fragments arranged by size: 3190 2037 1327 977 AgeI A'CCGG_T Cuts at: 6362 6362 Size: 7531 AhdI GACnn_n'nnGTC Cuts at: 6260 6260 Size: 7531 AleI CACnn'nnGTG Cuts at: 1166 2333 3590 4661 1166 Size: 1167 1257 1071 4036 Fragments arranged by size: 4036 1257 1167 1071 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2102 3575 1184 Size: 918 1473 5140 Fragments arranged by size: 5140 1473 918 AlwNI CAG_nnn'CTG Cuts at: 2505 3403 4721 5274 6846 2505 Size: 898 1318 553 1572 3190 Fragments arranged by size: 3190 1572 1318 898 553 ApaI G_GGCC'C Cuts at: 2032 2168 3010 3190 2032 Size: 136 842 180 6373 Fragments arranged by size: 6373 842 180 136 ApaLI G'TGCA_C Cuts at: 2326 4733 5588 6744 2326 Size: 2407 855 1156 3113 Fragments arranged by size: 3113 2407 1156 855 ApoI r'AATT_y Cuts at: 1493 4840 5066 5218 7327 1493 Size: 3347 226 152 2109 1697 Fragments arranged by size: 3347 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6085 160 Size: 872 5053 1606 Fragments arranged by size: 5053 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7531 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2113 2138 2161 2382 2581 2699 Size: 73 81 89 25 23 221 199 118 Cuts at: 2699 3020 3134 3326 3539 3887 4474 4696 4791 Size: 321 114 192 213 348 587 222 95 Cuts at: 4791 4856 1870 Size: 65 4545 Fragments arranged by size: 4545 587 348 321 222 221 213 199 192 118 114 95 89 81 73 65 25 23 AvrII C'CTAG_G Cuts at: 4826 4826 Size: 7531 BamHI G'GATC_C Cuts at: 2319 4861 5228 2319 Size: 2542 367 4622 Fragments arranged by size: 4622 2542 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2338 2434 2529 2831 2975 Size: 325 217 627 551 96 95 302 144 Cuts at: 2975 3910 3968 3987 4852 6307 618 Size: 935 58 19 865 1455 1842 Fragments arranged by size: 1842 1455 935 865 627 551 325 302 217 144 96 95 58 19 BanII G_rGCy'C Cuts at: 1391 2025 2032 2096 2161 2168 2391 3010 3178 Size: 634 7 64 65 7 223 619 168 Cuts at: 3178 3190 3894 4212 4475 4496 4683 4850 1391 Size: 12 704 318 263 21 187 167 4072 Fragments arranged by size: 4072 704 634 619 318 263 223 187 168 167 65 64 21 12 7 7 BbeI G_GCGC'C Cuts at: 2835 3972 2835 Size: 1137 6394 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2615 3850 928 Size: 971 716 1235 4609 Fragments arranged by size: 4609 1235 971 716 BbvCI CC'TCA_GC Cuts at: 4742 4742 Size: 7531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3981 4015 4234 4268 5722 5756 1903 Size: 34 2044 34 219 34 1454 34 3678 Fragments arranged by size: 3678 2044 1454 219 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5423 6633 5423 Size: 1210 6321 BclI T'GATC_A Cuts at: 1548 4707 1548 Size: 3159 4372 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3807 4172 Size: 107 122 71 631 605 337 1798 365 Cuts at: 4172 6142 136 Size: 1970 1525 Fragments arranged by size: 1970 1798 1525 631 605 365 337 122 107 71 BglII A'GATC_T Cuts at: 2907 3204 7526 2907 Size: 297 4322 2912 Fragments arranged by size: 4322 2912 297 BlpI GC'TnA_GC Cuts at: 4265 4948 4265 Size: 683 6848 Bme1580I G_kGCm'C Cuts at: 1790 2032 2168 2330 2490 2532 3010 3190 3915 Size: 242 136 162 160 42 478 180 725 Cuts at: 3915 4037 4704 4737 5592 6748 1790 Size: 122 667 33 855 1156 2573 Fragments arranged by size: 2573 1156 855 725 667 478 242 180 162 160 136 122 42 33 BmgBI CAC'GTC Cuts at: 1318 2613 2629 3370 3499 1318 Size: 1295 16 741 129 5350 Fragments arranged by size: 5350 1295 741 129 16 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4425 5262 6220 Size: 416 53 83 399 24 3008 837 958 Cuts at: 6220 442 Size: 1753 Fragments arranged by size: 3008 1753 958 837 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7531 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 4661 3836 Size: 825 6706 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 3011 3059 3308 3995 4565 6191 1239 Size: 1211 561 48 249 687 570 1626 2579 Fragments arranged by size: 2579 1626 1211 687 570 561 249 48 Bpu10I CC'TnA_GC Cuts at: 2095 4742 2095 Size: 2647 4884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3206 3788 4634 5656 6521 6819 3206 Size: 582 846 1022 865 298 3918 Fragments arranged by size: 3918 1022 865 846 582 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3807 6194 882 Size: 617 2308 2387 2219 Fragments arranged by size: 2387 2308 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2314 3241 3469 4342 5322 493 Size: 711 1110 927 228 873 980 2702 Fragments arranged by size: 2702 1110 980 927 873 711 228 BsaBI GATnn'nnATC Cuts at: 7525 7525 Size: 7531 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 2274 2716 2832 Size: 53 83 186 936 618 123 442 116 Cuts at: 2832 3078 3969 4128 4566 5338 5720 6355 275 Size: 246 891 159 438 772 382 635 1451 Fragments arranged by size: 1451 936 891 772 635 618 442 438 382 246 186 159 123 116 83 53 BsaWI w'CCGG_w Cuts at: 2193 2881 3831 4604 4611 5964 6362 6636 6783 Size: 688 950 773 7 1353 398 274 147 Cuts at: 6783 7194 2193 Size: 411 2530 Fragments arranged by size: 2530 1353 950 773 688 411 398 274 147 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 2427 2457 3596 Size: 30 165 30 816 30 178 30 1139 Cuts at: 3596 3626 3887 3917 3971 4001 4082 4112 1178 Size: 30 261 30 54 30 81 30 4597 Fragments arranged by size: 4597 1139 816 261 178 165 81 54 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2611 2996 3134 3557 3604 3992 4310 4637 Size: 1425 385 138 423 47 388 318 327 Cuts at: 4637 4780 1186 Size: 143 3937 Fragments arranged by size: 3937 1425 423 388 385 327 318 143 138 47 BseYI C'CCAG_C Cuts at: 2041 2089 2342 3086 3098 3957 4065 4463 4467 Size: 48 253 744 12 859 108 398 4 Cuts at: 4467 6734 7230 2041 Size: 2267 496 2342 Fragments arranged by size: 2342 2267 859 744 496 398 253 108 48 12 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2450 3722 4203 4244 4307 4367 4685 Size: 573 714 1272 481 41 63 60 318 Cuts at: 4685 1163 Size: 4009 Fragments arranged by size: 4009 1272 714 573 481 318 63 60 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 3310 4981 5742 6770 1540 Size: 275 186 18 1291 1671 761 1028 2301 Fragments arranged by size: 2301 1671 1291 1028 761 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2330 3269 3726 4137 4737 5592 5677 6748 2330 Size: 939 457 411 600 855 85 1071 3113 Fragments arranged by size: 3113 1071 939 855 600 457 411 85 BsmI GAATG_Cn' Cuts at: 5042 5135 5042 Size: 93 7438 BsmBI CGTCTCn'nnnn_ Cuts at: 3505 3505 Size: 7531 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2032 2096 2161 2168 2330 2391 Size: 399 235 7 64 65 7 162 61 Cuts at: 2391 2490 2532 3010 3178 3190 3269 3726 3894 Size: 99 42 478 168 12 79 457 168 Cuts at: 3894 3915 4037 4137 4212 4475 4496 4683 4704 Size: 21 122 100 75 263 21 187 21 Cuts at: 4704 4737 4850 5592 5677 6748 1391 Size: 33 113 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 457 399 263 235 187 168 168 162 122 113 100 99 85 79 75 65 64 61 42 33 21 21 21 12 7 7 BspEI T'CCGG_A Cuts at: 2193 2881 3831 4604 4611 7194 2193 Size: 688 950 773 7 2583 2530 Fragments arranged by size: 2583 2530 950 773 688 7 BspHI T'CATG_A Cuts at: 2293 5420 7150 2293 Size: 3127 1730 2674 Fragments arranged by size: 3127 2674 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2583 3436 3741 3825 4393 4657 4886 844 Size: 1739 853 305 84 568 264 229 3489 Fragments arranged by size: 3489 1739 853 568 305 264 229 84 BsrBI CCG'CTC Cuts at: 1394 1823 2143 3276 3664 4411 5418 1394 Size: 429 320 1133 388 747 1007 3507 Fragments arranged by size: 3507 1133 1007 747 429 388 320 BsrDI GCAATG_nn' Cuts at: 65 1248 6026 6200 65 Size: 1183 4778 174 1396 Fragments arranged by size: 4778 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2258 2437 2636 2972 3048 3363 3411 Size: 110 448 179 199 336 76 315 48 Cuts at: 3411 3702 6175 6362 1700 Size: 291 2473 187 2869 Fragments arranged by size: 2869 2473 448 336 315 291 199 187 179 110 76 48 BsrGI T'GTAC_A Cuts at: 96 3669 4572 96 Size: 3573 903 3055 Fragments arranged by size: 3573 3055 903 BssHII G'CGCG_C Cuts at: 1925 2106 2108 2588 1925 Size: 181 2 480 6868 Fragments arranged by size: 6868 480 181 2 BssSI C'ACGA_G Cuts at: 2329 4169 5591 6603 2329 Size: 1840 1422 1012 3257 Fragments arranged by size: 3257 1840 1422 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2505 794 Size: 1711 5820 BstBI TT'CG_AA Cuts at: 6335 6335 Size: 7531 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7531 BstXI CCAn_nnnn'nTGG Cuts at: 2525 3162 4159 4175 2525 Size: 637 997 16 5881 Fragments arranged by size: 5881 997 637 16 BstYI r'GATC_y Cuts at: 1972 2319 2907 3204 4392 4861 5228 5613 5630 Size: 347 588 297 1188 469 367 385 17 Cuts at: 5630 7082 7168 7180 7526 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1188 588 469 385 367 347 346 297 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4728 4728 Size: 7531 BtgI C'CryG_G Cuts at: 513 1065 2004 2685 2805 2850 3182 3588 513 Size: 552 939 681 120 45 332 406 4456 Fragments arranged by size: 4456 939 681 552 406 332 120 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3368 3957 487 Size: 683 56 2142 589 4061 Fragments arranged by size: 4061 2142 683 589 56 BtsI GCAGTG_nn' Cuts at: 2368 2446 2506 2999 3711 4186 5047 5841 5861 Size: 78 60 493 712 475 861 794 20 Cuts at: 5861 2368 Size: 4038 Fragments arranged by size: 4038 861 794 712 493 475 78 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7531 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7496 DraI TTT'AAA Cuts at: 4835 4835 Size: 7531 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6538 809 Size: 507 5222 1802 Fragments arranged by size: 5222 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2256 2634 Size: 54 1425 50 275 18 186 240 378 Cuts at: 2634 2970 3031 3307 4017 4978 5867 7496 8 Size: 336 61 276 710 961 889 1629 43 Fragments arranged by size: 1629 1425 961 889 710 378 336 276 275 240 186 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 3307 4978 1537 Size: 275 204 1291 1671 4090 Fragments arranged by size: 4090 1671 1291 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 3257 3888 4287 5461 1353 Size: 1904 631 399 1174 3423 Fragments arranged by size: 3423 1904 1174 631 399 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2936 4811 6118 6492 6638 387 Size: 1064 138 1347 1875 1307 374 146 1280 Fragments arranged by size: 1875 1347 1307 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2450 2755 2924 3011 3059 3221 Size: 393 695 123 305 169 87 48 162 Cuts at: 3221 3308 3701 3995 4565 5594 6191 6978 7246 Size: 87 393 294 570 1029 597 787 268 Cuts at: 7246 1239 Size: 1524 Fragments arranged by size: 1524 1029 787 695 597 570 393 393 305 294 268 169 162 123 87 87 48 EcoO109I rG'GnC_Cy Cuts at: 2028 2135 2165 2425 3025 3196 3292 3878 4090 Size: 107 30 260 600 171 96 586 212 Cuts at: 4090 4693 2028 Size: 603 4866 Fragments arranged by size: 4866 603 600 586 260 212 171 107 96 30 EcoRI G'AATT_C Cuts at: 4840 5218 4840 Size: 378 7153 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 7531 FspI TGC'GCA Cuts at: 6037 6037 Size: 7531 HaeII r_GCGC'y Cuts at: 1678 2444 2693 2835 2847 3972 6678 1678 Size: 766 249 142 12 1125 2706 2531 Fragments arranged by size: 2706 2531 1125 766 249 142 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4082 4114 6172 6204 6246 Size: 32 135 32 2510 32 2058 32 42 Cuts at: 6246 6278 1373 Size: 32 2626 Fragments arranged by size: 2626 2510 2058 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4875 5121 669 Size: 988 281 2937 246 3079 Fragments arranged by size: 3079 2937 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7531 HpaI GTT'AAC Cuts at: 5121 5121 Size: 7531 KasI G'GCGC_C Cuts at: 2831 3968 2831 Size: 1137 6394 KpnI G_GTAC'C Cuts at: 3991 4856 3991 Size: 865 6666 MfeI C'AATT_G Cuts at: 5130 5130 Size: 7531 MluI A'CGCG_T Cuts at: 7407 7407 Size: 7531 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2262 3615 4521 6645 6829 1305 Size: 957 1353 906 2124 184 2007 Fragments arranged by size: 2124 2007 1353 957 906 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3033 4019 10 Size: 54 1425 343 1201 986 3522 Fragments arranged by size: 3522 1425 1201 986 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2523 3590 3692 4661 5489 5848 Size: 648 1167 190 1067 102 969 828 359 Cuts at: 5848 6007 518 Size: 159 2042 Fragments arranged by size: 2042 1167 1067 969 828 648 359 190 159 102 MspA1I CmG'CkG Cuts at: 1606 2171 2687 2807 3184 3301 3340 3376 3762 Size: 565 516 120 377 117 39 36 386 Cuts at: 3762 4065 4083 4146 4467 4718 4947 5624 6772 Size: 303 18 63 321 251 229 677 1148 Cuts at: 6772 7017 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 565 516 386 377 321 303 251 245 229 120 117 63 39 36 18 NaeI GCC'GGC Cuts at: 2260 2638 3050 3365 3704 2260 Size: 378 412 315 339 6087 Fragments arranged by size: 6087 412 378 339 315 NarI GG'CG_CC Cuts at: 2832 3969 2832 Size: 1137 6394 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6040 Fragments arranged by size: 6040 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7531 NgoMIV G'CCGG_C Cuts at: 2258 2636 3048 3363 3702 2258 Size: 378 412 315 339 6087 Fragments arranged by size: 6087 412 378 339 315 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7531 NotI GC'GGCC_GC Cuts at: 4978 4978 Size: 7531 NspI r_CATG'y Cuts at: 1132 1229 3676 4741 4889 6434 1132 Size: 97 2447 1065 148 1545 2229 Fragments arranged by size: 2447 2229 1545 1065 148 97 PasI CC'CwG_GG Cuts at: 1348 4030 4621 4683 1348 Size: 2682 591 62 4196 Fragments arranged by size: 4196 2682 591 62 PciI A'CATG_T Cuts at: 6430 6430 Size: 7531 PflMI CCAn_nnn'nTGG Cuts at: 1108 3343 1108 Size: 2235 5296 PfoI T'CCnGG_A Cuts at: 2427 2888 2427 Size: 461 7070 PmeI GTTT'AAAC Cuts at: 4835 4835 Size: 7531 PmlI CAC'GTG Cuts at: 2314 2314 Size: 7531 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2564 2596 4079 4111 5600 5632 7113 7145 2564 Size: 32 1483 32 1489 32 1481 32 2950 Fragments arranged by size: 2950 1489 1483 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2135 2425 3025 3196 3292 3878 4090 2135 Size: 290 600 171 96 586 212 5576 Fragments arranged by size: 5576 600 586 290 212 171 96 PshAI GACnn'nnGTC Cuts at: 1613 3762 4050 4565 1613 Size: 2149 288 515 4579 Fragments arranged by size: 4579 2149 515 288 PsiI TTA'TAA Cuts at: 5101 5101 Size: 7531 PspOMI G'GGCC_C Cuts at: 2028 2164 3006 3186 2028 Size: 136 842 180 6373 Fragments arranged by size: 6373 842 180 136 PstI C_TGCA'G Cuts at: 830 2081 2501 2606 3827 4359 4404 4883 830 Size: 1251 420 105 1221 532 45 479 3478 Fragments arranged by size: 3478 1251 1221 532 479 420 105 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7531 PvuII CAG'CTG Cuts at: 3340 3762 4467 4718 3340 Size: 422 705 251 6153 Fragments arranged by size: 6153 705 422 251 SacII CC_GC'GG Cuts at: 2688 2808 3185 2688 Size: 120 377 7034 Fragments arranged by size: 7034 377 120 SalI G'TCGA_C Cuts at: 1936 4873 1936 Size: 2937 4594 SanDI GG'GwC_CC Cuts at: 2135 2135 Size: 7531 SapI GCTCTTCn'nnn_ Cuts at: 3257 3257 Size: 7531 SbfI CC_TGCA'GG Cuts at: 4883 4883 Size: 7531 ScaI AGT'ACT Cuts at: 5779 7491 5779 Size: 1712 5819 SexAI A'CCwGG_T Cuts at: 4508 4508 Size: 7531 SfcI C'TryA_G Cuts at: 826 1045 2077 2361 2497 2602 3416 3823 4078 Size: 219 1032 284 136 105 814 407 255 Cuts at: 4078 4355 4400 4879 6014 6695 6886 826 Size: 277 45 479 1135 681 191 1471 Fragments arranged by size: 1471 1135 1032 814 681 479 407 284 277 255 219 191 136 105 45 SfiI GGCCn_nnn'nGGCC Cuts at: 3807 3807 Size: 7531 SfoI GGC'GCC Cuts at: 2833 3970 2833 Size: 1137 6394 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7531 SgrAI Cr'CCGG_yG Cuts at: 1700 2437 1700 Size: 737 6794 SmaI CCC'GGG Cuts at: 1872 2026 2115 2163 2384 3136 3889 4698 4793 Size: 154 89 48 221 752 753 809 95 Cuts at: 4793 4858 1872 Size: 65 4545 Fragments arranged by size: 4545 809 753 752 221 154 95 89 65 48 SmlI C'TyrA_G Cuts at: 820 1017 1943 3221 3803 4649 5635 6536 6798 Size: 197 926 1278 582 846 986 901 262 Cuts at: 6798 820 Size: 1553 Fragments arranged by size: 1553 1278 986 926 901 846 582 262 197 SnaBI TAC'GTA Cuts at: 493 5322 493 Size: 4829 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7531 SphI G_CATG'C Cuts at: 1132 4889 1132 Size: 3757 3774 SrfI GCCC'GGGC Cuts at: 4793 4793 Size: 7531 SspI AAT'ATT Cuts at: 5 52 5455 5 Size: 47 5403 2081 Fragments arranged by size: 5403 2081 47 StuI AGG'CCT Cuts at: 1517 3071 3161 3801 4443 1517 Size: 1554 90 640 642 4605 Fragments arranged by size: 4605 1554 642 640 90 StyI C'CwwG_G Cuts at: 513 1065 2004 3619 4048 4826 4970 513 Size: 552 939 1615 429 778 144 3074 Fragments arranged by size: 3074 1615 939 778 552 429 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2356 2490 2561 3949 5558 5575 5728 Size: 1255 776 134 71 1388 1609 17 153 Cuts at: 5728 5913 6358 325 Size: 185 445 1498 Fragments arranged by size: 1609 1498 1388 1255 776 445 185 153 134 71 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3546 3669 4572 Size: 274 80 33 51 1443 1569 123 903 Cuts at: 4572 5777 7489 96 Size: 1205 1712 138 Fragments arranged by size: 1712 1569 1443 1205 903 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2219 2251 2427 2459 3596 3628 3887 3919 7491 Size: 32 176 32 1137 32 259 32 3572 Cuts at: 7491 7523 2219 Size: 32 2227 Fragments arranged by size: 3572 2227 1137 259 176 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7531 XbaI T'CTAG_A Cuts at: 4867 4867 Size: 7531 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3586 4027 4589 3586 Size: 441 562 6528 Fragments arranged by size: 6528 562 441 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7531 XmaI C'CCGG_G Cuts at: 1870 2024 2113 2161 2382 3134 3887 4696 4791 Size: 154 89 48 221 752 753 809 95 Cuts at: 4791 4856 1870 Size: 65 4545 Fragments arranged by size: 4545 809 753 752 221 154 95 89 65 48 XmnI GAAnn'nnTTC Cuts at: 5660 5660 Size: 7531 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2275 5339 276 Size: 53 83 186 936 741 3064 2468 Fragments arranged by size: 3064 2468 936 741 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SwaI