Restriction map for
pFN21AB1325
(Circular) (Six-base) MAPSORT of: pFN21AB1325.seq Check: 3790 from: 1 to: 6967
pFN21AB1325 6967 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2083 2620 2083
Size: 537 6430
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4777 278
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2012 2496 4310 1937
Size: 75 484 1814 4594
Fragments arranged by size:
4594 1814 484 75
Acc65I G'GTAC_C
Cuts at: 4288 4288
Size: 6967
AclI AA'CG_TT
Cuts at: 1563 5094 5467 1563
Size: 3531 373 3063
Fragments arranged by size:
3531 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2317 2386 3422 5030 6414 6682 1632
Size: 685 69 1036 1608 1384 268 1917
Fragments arranged by size:
1917 1608 1384 1036 685 268 69
AfeI AGC'GCT
Cuts at: 3182 3182
Size: 6967
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6770
AflIII A'CryG_T
Cuts at: 1203 5866 6843 1203
Size: 4663 977 1327
Fragments arranged by size:
4663 1327 977
AgeI A'CCGG_T
Cuts at: 5798 5798
Size: 6967
AhdI GACnn_n'nnGTC
Cuts at: 5696 5696
Size: 6967
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6967
AlwNI CAG_nnn'CTG
Cuts at: 2375 4710 6282 2375
Size: 2335 1572 3060
Fragments arranged by size:
3060 2335 1572
ApaI G_GGCC'C
Cuts at: 3302 3302
Size: 6967
ApaLI G'TGCA_C
Cuts at: 5024 6180 5024
Size: 1156 5811
ApoI r'AATT_y
Cuts at: 1493 4276 4502 4654 6763 1493
Size: 2783 226 152 2109 1697
Fragments arranged by size:
2783 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5521 160
Size: 872 4489 1606
Fragments arranged by size:
4489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
AvaI C'yCGr_G
Cuts at: 1870 1943 2573 2746 4292 1870
Size: 73 630 173 1546 4545
Fragments arranged by size:
4545 1546 630 173 73
BamHI G'GATC_C
Cuts at: 3490 3699 4297 4664 3490
Size: 209 598 367 5793
Fragments arranged by size:
5793 598 367 209
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 3709 4288 5743 618
Size: 325 217 627 1196 726 579 1455 1842
Fragments arranged by size:
1842 1455 1196 726 627 579 325 217
BanII G_rGCy'C
Cuts at: 1391 2297 2538 2943 3302 4124 4286 1391
Size: 906 241 405 359 822 162 4072
Fragments arranged by size:
4072 906 822 405 359 241 162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3233 3239 928
Size: 971 1334 6 4656
Fragments arranged by size:
4656 1334 971 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 5158 5192 1903
Size: 34 1808 34 1379 34 3678
Fragments arranged by size:
3678 1808 1379 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3464 4859 6069 3464
Size: 1395 1210 4362
Fragments arranged by size:
4362 1395 1210
BclI T'GATC_A
Cuts at: 1548 3799 1548
Size: 2251 4716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 3995 4227
Size: 107 122 71 631 605 535 1788 232
Cuts at: 4227 5578 136
Size: 1351 1525
Fragments arranged by size:
1788 1525 1351 631 605 535 232 122
107 71
BglII A'GATC_T
Cuts at: 2874 3087 6962 2874
Size: 213 3875 2879
Fragments arranged by size:
3875 2879 213
BlpI GC'TnA_GC
Cuts at: 4029 4384 4029
Size: 355 6612
Bme1580I G_kGCm'C
Cuts at: 1790 3302 5028 6184 1790
Size: 1512 1726 1156 2573
Fragments arranged by size:
2573 1726 1512 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6967
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2225 2363 4018
Size: 416 53 83 399 24 808 138 1655
Cuts at: 4018 4698 5656 442
Size: 680 958 1753
Fragments arranged by size:
1753 1655 958 808 680 416 399 138
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6967
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 3106
Size: 6967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3383 5627 1239
Size: 2144 2244 2579
Fragments arranged by size:
2579 2244 2144
Bpu10I CC'TnA_GC
Cuts at: 2255 2537 3392 3602 2255
Size: 282 855 210 5620
Fragments arranged by size:
5620 855 282 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2311 3101 3965 5092 5957 6255 2311
Size: 790 864 1127 865 298 3023
Fragments arranged by size:
3023 1127 865 864 790 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2513 3983 5630 882
Size: 617 1014 1470 1647 2219
Fragments arranged by size:
2219 1647 1470 1014 617
BsaAI yAC'GTr
Cuts at: 493 1204 4758 493
Size: 711 3554 2702
Fragments arranged by size:
3554 2702 711
BsaBI GATnn'nnATC
Cuts at: 6961 6961
Size: 6967
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3192 4774 5156 5791
Size: 53 83 186 936 1659 1582 382 635
Cuts at: 5791 275
Size: 1451
Fragments arranged by size:
1659 1582 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2792 5400 5798 6072 6219 6630 2792
Size: 2608 398 274 147 411 3129
Fragments arranged by size:
3129 2608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 3710 3740 1178
Size: 30 165 30 642 30 1635 30 4405
Fragments arranged by size:
4405 1635 642 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2435 2546 2861 2951 3718 1186
Size: 868 381 111 315 90 767 4435
Fragments arranged by size:
4435 868 767 381 315 111 90
BseYI C'CCAG_C
Cuts at: 2130 2391 2818 2830 6170 6666 2130
Size: 261 427 12 3340 496 2431
Fragments arranged by size:
3340 2431 496 427 261 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2121 1163
Size: 573 385 6009
Fragments arranged by size:
6009 573 385
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3696 4417 5178 6206 1540
Size: 275 186 1695 721 761 1028 2301
Fragments arranged by size:
2301 1695 1028 761 721 275 186
BsiHKAI G_wGCw'C
Cuts at: 2297 2538 2943 3398 4035 4103 4124 5028 5113
Size: 241 405 455 637 68 21 904 85
Cuts at: 5113 6184 2297
Size: 1071 3080
Fragments arranged by size:
3080 1071 904 637 455 405 241 85
68 21
BsmI GAATG_Cn'
Cuts at: 4478 4571 4478
Size: 93 6874
BsmBI CGTCTCn'nnnn_
Cuts at: 2882 2882
Size: 6967
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2297 2538 2943 3302 3398 4035 4103
Size: 399 507 241 405 359 96 637 68
Cuts at: 4103 4124 4286 5028 5113 6184 1391
Size: 21 162 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 637 507 405 399 359
241 162 96 85 68 21
BspEI T'CCGG_A
Cuts at: 6630 6630
Size: 6967
BspHI T'CATG_A
Cuts at: 4856 6586 4856
Size: 1730 5237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 2620 4322 844
Size: 1239 537 1702 3489
Fragments arranged by size:
3489 1702 1239 537
BsrBI CCG'CTC
Cuts at: 1394 1823 4854 1394
Size: 429 3031 3507
Fragments arranged by size:
3507 3031 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5462 5636 65
Size: 1183 4214 174 1396
Fragments arranged by size:
4214 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3928 5611 5798 1700
Size: 110 2118 1683 187 2869
Fragments arranged by size:
2869 2118 1683 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6967
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6967
BssSI C'ACGA_G
Cuts at: 5027 6039 5027
Size: 1012 5955
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6967
BstBI TT'CG_AA
Cuts at: 5771 5771
Size: 6967
BstEII G'GTnAC_C
Cuts at: 1184 2635 1184
Size: 1451 5516
BstXI CCAn_nnnn'nTGG
Cuts at: 3502 3502
Size: 6967
BstYI r'GATC_y
Cuts at: 1972 2237 2874 3087 3490 3699 4297 4664 5049
Size: 265 637 213 403 209 598 367 385
Cuts at: 5049 5066 6518 6604 6616 6962 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 637 598 403 385 367 346
265 213 209 86 17 12
BstZ17I GTA'TAC
Cuts at: 2497 2497
Size: 6967
BtgI C'CryG_G
Cuts at: 513 1065 2298 2457 3030 3645 3839 3858 513
Size: 552 1233 159 573 615 194 19 3622
Fragments arranged by size:
3622 1233 615 573 552 194 159 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3946 487
Size: 683 56 2720 3508
Fragments arranged by size:
3508 2720 683 56
BtsI GCAGTG_nn'
Cuts at: 3525 3588 4483 5277 5297 3525
Size: 63 895 794 20 5195
Fragments arranged by size:
5195 895 794 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6967
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6932
DraI TTT'AAA
Cuts at: 4271 4271
Size: 6967
DraIII CAC_nnn'GTG
Cuts at: 2956 2956
Size: 6967
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5974 809
Size: 507 4658 1802
Fragments arranged by size:
4658 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 2380 2460
Size: 54 1425 50 275 18 289 261 80
Cuts at: 2460 2898 3693 3836 4414 5303 6932 8
Size: 438 795 143 578 889 1629 43
Fragments arranged by size:
1629 1425 889 795 578 438 289 275
261 143 80 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3693 4414 1537
Size: 275 1881 721 4090
Fragments arranged by size:
4090 1881 721 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4076 4897 1353
Size: 2723 821 3423
Fragments arranged by size:
3423 2723 821
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5554 5928 6074 387
Size: 1064 138 3965 374 146 1280
Fragments arranged by size:
3965 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2295 2536 2941 4122 2295
Size: 241 405 1181 5140
Fragments arranged by size:
5140 1181 405 241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2386 3383 3422 5030 5627 6414
Size: 393 685 69 997 39 1608 597 787
Cuts at: 6414 6682 1239
Size: 268 1524
Fragments arranged by size:
1608 1524 997 787 685 597 393 268
69 39
EcoO109I rG'GnC_Cy
Cuts at: 2044 2397 2594 3299 2044
Size: 353 197 705 5712
Fragments arranged by size:
5712 705 353 197
EcoRI G'AATT_C
Cuts at: 4276 4654 4276
Size: 378 6589
EcoRV GAT'ATC
Cuts at: 2798 2798
Size: 6967
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2259 2629 2259
Size: 370 6597
FspI TGC'GCA
Cuts at: 5473 5473
Size: 6967
HaeII r_GCGC'y
Cuts at: 1678 2929 3184 6114 1678
Size: 1251 255 2930 2531
Fragments arranged by size:
2930 2531 1251 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2369 2401 2782
Size: 32 135 32 473 32 292 32 381
Cuts at: 2782 2814 3079 3108 3111 3140 5608 5640 5682
Size: 32 265 29 3 29 2468 32 42
Cuts at: 5682 5714 1373
Size: 32 2626
Fragments arranged by size:
2626 2468 473 381 292 265 135 42
32 32 32 32 32 32 32 29
29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2013 4311 4557 669
Size: 988 281 75 2298 246 3079
Fragments arranged by size:
3079 2298 988 281 246 75
HindIII A'AGCT_T
Cuts at: 748 2262 748
Size: 1514 5453
HpaI GTT'AAC
Cuts at: 4557 4557
Size: 6967
KpnI G_GTAC'C
Cuts at: 4292 4292
Size: 6967
MfeI C'AATT_G
Cuts at: 4566 4566
Size: 6967
MluI A'CGCG_T
Cuts at: 6843 6843
Size: 6967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2724 6081 6265 1305
Size: 1419 3357 184 2007
Fragments arranged by size:
3357 2007 1419 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2121 2382 2900 10
Size: 54 1425 343 289 261 518 4077
Fragments arranged by size:
4077 1425 518 343 289 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4925 5284 5443 518
Size: 648 3759 359 159 2042
Fragments arranged by size:
3759 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2818 4218 4383 5060 6208 6453 1606
Size: 1212 1400 165 677 1148 245 2120
Fragments arranged by size:
2120 1400 1212 1148 677 245 165
NcoI C'CATG_G
Cuts at: 513 1065 2298 3645 3839 513
Size: 552 1233 1347 194 3641
Fragments arranged by size:
3641 1347 1233 552 194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6967
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6967
NotI GC'GGCC_GC
Cuts at: 4414 4414
Size: 6967
NspI r_CATG'y
Cuts at: 1132 1229 4325 5870 1132
Size: 97 3096 1545 2229
Fragments arranged by size:
3096 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2598 3949 4048 1348
Size: 1250 1351 99 4267
Fragments arranged by size:
4267 1351 1250 99
PciI A'CATG_T
Cuts at: 5866 5866
Size: 6967
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2997 1108
Size: 1889 5078
PfoI T'CCnGG_A
Cuts at: 2616 3360 2616
Size: 744 6223
PmeI GTTT'AAAC
Cuts at: 4271 4271
Size: 6967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 6549 6581 5036
Size: 32 1481 32 5422
Fragments arranged by size:
5422 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2397 2594 2044
Size: 353 197 6417
Fragments arranged by size:
6417 353 197
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6967
PsiI TTA'TAA
Cuts at: 4537 4537
Size: 6967
PspOMI G'GGCC_C
Cuts at: 3298 3298
Size: 6967
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 6935
PstI C_TGCA'G
Cuts at: 830 2094 2631 2976 3532 3708 4319 830
Size: 1264 537 345 556 176 611 3478
Fragments arranged by size:
3478 1264 611 556 537 345 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6967
PvuII CAG'CTG
Cuts at: 2818 2818
Size: 6967
SacI G_AGCT'C
Cuts at: 2297 2538 2943 4124 2297
Size: 241 405 1181 5140
Fragments arranged by size:
5140 1181 405 241
SalI G'TCGA_C
Cuts at: 1936 2011 4309 1936
Size: 75 2298 4594
Fragments arranged by size:
4594 2298 75
SapI GCTCTTCn'nnn_
Cuts at: 4076 4076
Size: 6967
SbfI CC_TGCA'GG
Cuts at: 2094 2631 3708 4319 2094
Size: 537 1077 611 4742
Fragments arranged by size:
4742 1077 611 537
ScaI AGT'ACT
Cuts at: 5215 6927 5215
Size: 1712 5255
SexAI A'CCwGG_T
Cuts at: 2046 3147 2046
Size: 1101 5866
SfcI C'TryA_G
Cuts at: 826 1045 2090 2627 2972 3528 3704 3910 4315
Size: 219 1045 537 345 556 176 206 405
Cuts at: 4315 5450 6131 6322 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 681 556 537 405 345
219 206 191 176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6967
SmaI CCC'GGG
Cuts at: 1872 4294 1872
Size: 2422 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2290 2746 3080 3980 5071 5972
Size: 197 926 347 456 334 900 1091 901
Cuts at: 5972 6234 820
Size: 262 1553
Fragments arranged by size:
1553 1091 926 901 900 456 347 334
262 197
SnaBI TAC'GTA
Cuts at: 493 4758 493
Size: 4265 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6967
SphI G_CATG'C
Cuts at: 1132 4325 1132
Size: 3193 3774
SspI AAT'ATT
Cuts at: 5 52 4891 5
Size: 47 4839 2081
Fragments arranged by size:
4839 2081 47
StuI AGG'CCT
Cuts at: 1517 3740 3866 4134 1517
Size: 2223 126 268 4350
Fragments arranged by size:
4350 2223 268 126
StyI C'CwwG_G
Cuts at: 513 1065 2298 2604 2979 3435 3495 3645 3735
Size: 552 1233 306 375 456 60 150 90
Cuts at: 3735 3839 4194 4406 513
Size: 104 355 212 3074
Fragments arranged by size:
3074 1233 552 456 375 355 306 212
150 104 90 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3748 3896 4000 4249 4994 5011 5164
Size: 1255 2168 148 104 249 745 17 153
Cuts at: 5164 5349 5794 325
Size: 185 445 1498
Fragments arranged by size:
2168 1498 1255 745 445 249 185 153
148 104 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3900 5213 6925
Size: 274 80 33 51 1443 1923 1313 1712
Cuts at: 6925 96
Size: 138
Fragments arranged by size:
1923 1712 1443 1313 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 3517 3549 6927 6959 2205
Size: 32 1280 32 3378 32 2213
Fragments arranged by size:
3378 2213 1280 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6967
XbaI T'CTAG_A
Cuts at: 4303 4303
Size: 6967
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2551 3484 2551
Size: 933 6034
XhoI C'TCGA_G
Cuts at: 1943 2746 1943
Size: 803 6164
XmaI C'CCGG_G
Cuts at: 1870 4292 1870
Size: 2422 4545
XmnI GAAnn'nnTTC
Cuts at: 5096 5096
Size: 6967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4775 276
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI RsrII SacII SanDI
SfiI SfoI SrfI SwaI