(Circular) (Six-base) MAPSORT of: pFN21AB0356.seq Check: 241 from: 1 to: 7909 pFN21AB0356 7909 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5719 278 Size: 53 83 186 936 4183 2468 Fragments arranged by size: 4183 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2624 5252 1937 Size: 687 2628 4594 Fragments arranged by size: 4594 2628 687 Acc65I G'GTAC_C Cuts at: 5230 5230 Size: 7909 AclI AA'CG_TT Cuts at: 1563 2439 5208 6036 6409 1563 Size: 876 2769 828 373 3063 Fragments arranged by size: 3063 2769 876 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2691 3986 5972 7356 7624 1632 Size: 1059 1295 1986 1384 268 1917 Fragments arranged by size: 1986 1917 1384 1295 1059 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7712 AflIII A'CryG_T Cuts at: 1203 4697 4855 6808 7785 1203 Size: 3494 158 1953 977 1327 Fragments arranged by size: 3494 1953 1327 977 158 AgeI A'CCGG_T Cuts at: 6740 6740 Size: 7909 AhdI GACnn_n'nnGTC Cuts at: 4369 6638 4369 Size: 2269 5640 AleI CACnn'nnGTG Cuts at: 1166 3675 1166 Size: 2509 5400 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7909 AlwNI CAG_nnn'CTG Cuts at: 5652 7224 5652 Size: 1572 6337 ApaLI G'TGCA_C Cuts at: 3000 4608 5966 7122 3000 Size: 1608 1358 1156 3787 Fragments arranged by size: 3787 1608 1358 1156 ApoI r'AATT_y Cuts at: 1493 2246 2384 3015 3693 3727 4561 4581 4835 Size: 753 138 631 678 34 834 20 254 Cuts at: 4835 4931 5218 5444 5596 7705 1493 Size: 96 287 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 753 678 631 287 254 226 152 138 96 34 20 AseI AT'TA_AT Cuts at: 160 1032 2129 6463 160 Size: 872 1097 4334 1606 Fragments arranged by size: 4334 1606 1097 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 AvaI C'yCGr_G Cuts at: 1870 1943 3507 5234 1870 Size: 73 1564 1727 4545 Fragments arranged by size: 4545 1727 1564 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3297 3330 3297 Size: 33 7876 BamHI G'GATC_C Cuts at: 5239 5606 5239 Size: 367 7542 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5230 6685 618 Size: 325 217 627 3443 1455 1842 Fragments arranged by size: 3443 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4909 5228 1391 Size: 3518 319 4072 Fragments arranged by size: 4072 3518 319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6938 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6100 6134 1903 Size: 34 50 34 4079 34 3678 Fragments arranged by size: 4079 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3514 4706 5801 7011 3514 Size: 1192 1095 1210 4412 Fragments arranged by size: 4412 1210 1192 1095 BclI T'GATC_A Cuts at: 1548 1548 Size: 7909 BfrBI ATG'CAT Cuts at: 5201 5201 Size: 7909 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6520 136 Size: 107 122 71 631 605 4848 1525 Fragments arranged by size: 4848 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3063 7904 3063 Size: 4841 3068 BlpI GC'TnA_GC Cuts at: 5326 5326 Size: 7909 Bme1580I G_kGCm'C Cuts at: 1790 3004 3999 4612 5970 7126 1790 Size: 1214 995 613 1358 1156 2573 Fragments arranged by size: 2573 1358 1214 1156 995 613 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5640 6598 442 Size: 416 53 83 399 24 4223 958 1753 Fragments arranged by size: 4223 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7909 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3638 Size: 7909 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6569 1239 Size: 5330 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2211 6034 6899 7197 2211 Size: 3823 865 298 2923 Fragments arranged by size: 3823 2923 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6572 882 Size: 617 5073 2219 Fragments arranged by size: 5073 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3034 5700 493 Size: 711 1830 2666 2702 Fragments arranged by size: 2702 2666 1830 711 BsaBI GATnn'nnATC Cuts at: 5142 7903 5142 Size: 2761 5148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5716 6098 6733 275 Size: 53 83 186 936 4183 382 635 1451 Fragments arranged by size: 4183 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6342 6740 7014 7161 7572 6342 Size: 398 274 147 411 6679 Fragments arranged by size: 6679 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7684 Fragments arranged by size: 7684 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3108 4254 1186 Size: 1922 1146 4841 Fragments arranged by size: 4841 1922 1146 BseYI C'CCAG_C Cuts at: 7112 7608 7112 Size: 496 7413 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5359 6120 7148 1540 Size: 275 186 3358 761 1028 2301 Fragments arranged by size: 3358 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3004 4612 5970 6055 7126 3004 Size: 1608 1358 85 1071 3787 Fragments arranged by size: 3787 1608 1358 1071 85 BsmI GAATG_Cn' Cuts at: 2114 4919 5037 5420 5513 2114 Size: 2805 118 383 93 4510 Fragments arranged by size: 4510 2805 383 118 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3004 3999 4612 4909 5228 5970 6055 Size: 399 1214 995 613 297 319 742 85 Cuts at: 6055 7126 1391 Size: 1071 2174 Fragments arranged by size: 2174 1214 1071 995 742 613 399 319 297 85 BspEI T'CCGG_A Cuts at: 7572 7572 Size: 7909 BspHI T'CATG_A Cuts at: 5121 5798 7528 5121 Size: 677 1730 5502 Fragments arranged by size: 5502 1730 677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5264 844 Size: 4420 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5796 1394 Size: 429 3973 3507 Fragments arranged by size: 3973 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2499 4134 5058 6404 6578 65 Size: 1183 1251 1635 924 1346 174 1396 Fragments arranged by size: 1635 1396 1346 1251 1183 924 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6553 6740 1700 Size: 110 4743 187 2869 Fragments arranged by size: 4743 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7909 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7909 BssSI C'ACGA_G Cuts at: 3195 3612 5969 6981 3195 Size: 417 2357 1012 4123 Fragments arranged by size: 4123 2357 1012 417 BstAPI GCAn_nnn'nTGC Cuts at: 794 4435 794 Size: 3641 4268 BstBI TT'CG_AA Cuts at: 3073 4498 5071 6713 3073 Size: 1425 573 1642 4269 Fragments arranged by size: 4269 1642 1425 573 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7909 BstXI CCAn_nnnn'nTGG Cuts at: 3273 4055 3273 Size: 782 7127 BstYI r'GATC_y Cuts at: 1972 2337 2401 2458 3063 3616 3826 5175 5239 Size: 365 64 57 605 553 210 1349 64 Cuts at: 5239 5606 5991 6008 7460 7546 7558 7904 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1349 605 553 385 367 365 346 210 86 64 64 57 17 12 BstZ17I GTA'TAC Cuts at: 2625 2625 Size: 7909 Bsu36I CC'TnA_GG Cuts at: 4295 4295 Size: 7909 BtgI C'CryG_G Cuts at: 513 1065 3088 513 Size: 552 2023 5334 Fragments arranged by size: 5334 2023 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7170 Fragments arranged by size: 7170 683 56 BtsI GCAGTG_nn' Cuts at: 3454 4610 5425 6219 6239 3454 Size: 1156 815 794 20 5124 Fragments arranged by size: 5124 1156 815 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7909 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4326 4361 529 Size: 35 3762 35 4077 Fragments arranged by size: 4077 3762 35 35 DraI TTT'AAA Cuts at: 2155 2301 2760 4017 4408 5213 2155 Size: 146 459 1257 391 805 4851 Fragments arranged by size: 4851 1257 805 459 391 146 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6916 809 Size: 507 5600 1802 Fragments arranged by size: 5600 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3341 5356 6245 Size: 54 1425 50 275 18 1511 2015 889 Cuts at: 6245 7874 8 Size: 1629 43 Fragments arranged by size: 2015 1629 1511 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5356 1537 Size: 275 3544 4090 Fragments arranged by size: 4090 3544 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3477 4299 5000 5839 1353 Size: 2124 822 701 839 3423 Fragments arranged by size: 3423 2124 839 822 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6496 6870 7016 387 Size: 1064 138 4907 374 146 1280 Fragments arranged by size: 4907 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2691 3986 5972 6569 7356 7624 1239 Size: 393 1059 1295 1986 597 787 268 1524 Fragments arranged by size: 1986 1524 1295 1059 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2117 2117 Size: 7909 EcoO109I rG'GnC_Cy Cuts at: 2467 3916 2467 Size: 1449 6460 EcoRI G'AATT_C Cuts at: 3727 5218 5596 3727 Size: 1491 378 6040 Fragments arranged by size: 6040 1491 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2897 3150 2897 Size: 253 7656 FspI TGC'GCA Cuts at: 6415 6415 Size: 7909 HaeII r_GCGC'y Cuts at: 1678 7056 1678 Size: 5378 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2329 2361 3608 3640 4355 Size: 32 135 32 757 32 1247 32 715 Cuts at: 4355 4387 6550 6582 6624 6656 1373 Size: 32 2163 32 42 32 2626 Fragments arranged by size: 2626 2163 1247 757 715 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5253 5499 669 Size: 988 281 3315 246 3079 Fragments arranged by size: 3315 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7909 HpaI GTT'AAC Cuts at: 5499 5499 Size: 7909 KpnI G_GTAC'C Cuts at: 5234 5234 Size: 7909 MfeI C'AATT_G Cuts at: 3493 5508 3493 Size: 2015 5894 MluI A'CGCG_T Cuts at: 7785 7785 Size: 7909 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3408 7023 7207 1305 Size: 2103 3615 184 2007 Fragments arranged by size: 3615 2103 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3343 10 Size: 54 1425 343 1511 4576 Fragments arranged by size: 4576 1511 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2375 2646 3271 3675 4574 4702 4769 Size: 648 1209 271 625 404 899 128 67 Cuts at: 4769 5148 5867 6226 6385 518 Size: 379 719 359 159 2042 Fragments arranged by size: 2042 1209 899 719 648 625 404 379 359 271 159 128 67 MspA1I CmG'CkG Cuts at: 1606 5325 6002 7150 7395 1606 Size: 3719 677 1148 245 2120 Fragments arranged by size: 3719 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3088 513 Size: 552 2023 5334 Fragments arranged by size: 5334 2023 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7909 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7909 NotI GC'GGCC_GC Cuts at: 5356 5356 Size: 7909 NsiI A_TGCA'T Cuts at: 5203 5203 Size: 7909 NspI r_CATG'y Cuts at: 1132 1229 2374 3491 4089 4988 5201 5267 6812 Size: 97 1145 1117 598 899 213 66 1545 Cuts at: 6812 1132 Size: 2229 Fragments arranged by size: 2229 1545 1145 1117 899 598 213 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7909 PciI A'CATG_T Cuts at: 6808 6808 Size: 7909 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7909 PmeI GTTT'AAAC Cuts at: 5213 5213 Size: 7909 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5978 6010 7491 7523 5978 Size: 32 1481 32 6364 Fragments arranged by size: 6364 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 3916 2467 Size: 1449 6460 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7909 PsiI TTA'TAA Cuts at: 2993 4845 5479 2993 Size: 1852 634 5423 Fragments arranged by size: 5423 1852 634 PstI C_TGCA'G Cuts at: 830 4617 5261 830 Size: 3787 644 3478 Fragments arranged by size: 3787 3478 644 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7909 SalI G'TCGA_C Cuts at: 1936 5251 1936 Size: 3315 4594 SapI GCTCTTCn'nnn_ Cuts at: 3477 3477 Size: 7909 SbfI CC_TGCA'GG Cuts at: 5261 5261 Size: 7909 ScaI AGT'ACT Cuts at: 6157 7869 6157 Size: 1712 6197 SfcI C'TryA_G Cuts at: 826 1045 4247 4613 5257 6392 7073 7264 826 Size: 219 3202 366 644 1135 681 191 1471 Fragments arranged by size: 3202 1471 1135 681 644 366 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7909 SmaI CCC'GGG Cuts at: 1872 5236 1872 Size: 3364 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2226 6013 6914 7176 820 Size: 197 926 283 3787 901 262 1553 Fragments arranged by size: 3787 1553 926 901 283 262 197 SnaBI TAC'GTA Cuts at: 493 5700 493 Size: 5207 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7909 SphI G_CATG'C Cuts at: 1132 2374 3491 4988 5201 5267 1132 Size: 1242 1117 1497 213 66 3774 Fragments arranged by size: 3774 1497 1242 1117 213 66 SspI AAT'ATT Cuts at: 5 52 2431 3046 3869 3935 5833 5 Size: 47 2379 615 823 66 1898 2081 Fragments arranged by size: 2379 2081 1898 823 615 66 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7909 StyI C'CwwG_G Cuts at: 513 1065 3088 5348 513 Size: 552 2023 2260 3074 Fragments arranged by size: 3074 2260 2023 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5936 5953 6106 6291 6736 325 Size: 1255 4356 17 153 185 445 1498 Fragments arranged by size: 4356 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2410 3910 6155 Size: 274 80 33 51 1443 433 1500 2245 Cuts at: 6155 7867 96 Size: 1712 138 Fragments arranged by size: 2245 1712 1500 1443 433 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2082 2114 7869 7901 2082 Size: 32 5755 32 2090 Fragments arranged by size: 5755 2090 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7909 XbaI T'CTAG_A Cuts at: 3731 5245 3731 Size: 1514 6395 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7909 XmaI C'CCGG_G Cuts at: 1870 5234 1870 Size: 3364 4545 XmnI GAAnn'nnTTC Cuts at: 6038 6038 Size: 7909 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5717 276 Size: 53 83 186 936 4183 2468 Fragments arranged by size: 4183 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI