Restriction map for
pFN21AB0207
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbeI BcgI BciVI BfrI
BglI BlnI BsiWI BsmBI BspDI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstZ17I Bsu36I ClaI CpoI DraI
Eam1105I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
KspI MluI NarI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SnaBI Sse8387I SspI
StuI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 3049 GACGTC GACGT^C
AcsI 1673 AAATTT [GA]^AATT[CT]
AflIII 1454 ACGTGT A^C[GA][CT]GT
Alw44I 1312 GTGCAC G^TGCAC
Aor51HI 827 AGCGCT AGC^GCT
ApaLI 1312 GTGCAC G^TGCAC
ApoI 1673 AAATTT [GA]^AATT[CT]
AspI 547 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 698 GGATCC G^GATCC
BbvCI 1275 CCTCAGC CCTCAGC(-5/-2)
BclI 914 TGATCA T^GATCA
BsaBI 460 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstBI 162 TTCGAA TT^CGAA
BstEII 1432 GGTGACC G^GT[ACGT]ACC
BstPI 1432 GGTGACC G^GT[ACGT]ACC
DraIII 2059 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2704 CTCTTC CTCTTC(1/4)
Eco47III 827 AGCGCT AGC^GCT
EcoO65I 1432 GGTGACC G^GT[ACGT]ACC
FbaI 914 TGATCA T^GATCA
HgaI 2528 GACGC GACGC(5/10)
HindIII 1703 AAGCTT A^AGCTT
Ksp632I 2704 CTCTTC CTCTTC(1/4)
MamI 460 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2475 CAATTG C^AATTG
MunI 2475 CAATTG C^AATTG
NaeI 2956 GCCGGC GCC^GGC
NgoMIV 2956 GCCGGC G^CCGGC
NspV 162 TTCGAA TT^CGAA
PflFI 547 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2963 GGGACCC [GA]G^G[AT]CC[CT]
SexAI 784 ACCAGGT A^CC[AT]GGT
SfuI 162 TTCGAA TT^CGAA
SpeI 157 ACTAGT A^CTAGT
SphI 923 GCATGC GCATG^C
Tth111I 547 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 837 TCTAGA T^CTAGA
XcmI 2846 CCAATGCTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 2755 CGCG CG^CG
2935 CGCG
AccIII 1030 TCCGGA T^CCGGA
2549 TCCGGA
AcyI 2528 GACGCC G[GA]^CG[CT]C
3049 GACGTC
BsaHI 2528 GACGCC G[GA]^CG[CT]C
3049 GACGTC
BsaI 715 GAGACC GGTCTC(1/5)
1264 GAGACC
BseAI 1030 TCCGGA T^CCGGA
2549 TCCGGA
BsiEI 1469 CGGCCG CG[GA][CT]^CG
2958 CGGCCG
BsmAI 715 GAGAC GTCTC(1/5)
1264 GAGAC
BsmI 249 GAATGC GAATGC(1/-1)
1404 GAATGC
Bsp1407I 213 TGTACA T^GTACA
1577 TGTACA
BspEI 1030 TCCGGA T^CCGGA
2549 TCCGGA
BsrGI 213 TGTACA T^GTACA
1577 TGTACA
BssSI 367 CTCGTG CACGAG(-5/-1)
1922 CTCGTG
BstAPI 124 GCACCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1802 GCATGTCCTGC
BstUI 2755 CGCG CG^CG
2935 CGCG
BstXI 645 CCAGCATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1382 CCACTGACGTGG
DraII 2129 AGGCCCC [GA]G^G[ACGT]CC[CT]
2963 GGGACCC
EagI 1469 CGGCCG C^GGCCG
2958 CGGCCG
EclXI 1469 CGGCCG C^GGCCG
2958 CGGCCG
Eco52I 1469 CGGCCG C^GGCCG
2958 CGGCCG
EcoO109I 2129 AGGCCCC [GA]G^G[ACGT]CC[CT]
2963 GGGACCC
HaeII 827 AGCGCT [GA]GCGC^[CT]
3020 AGCGCC
Hin1I 2528 GACGCC G[GA]^CG[CT]C
3049 GACGTC
MroI 1030 TCCGGA T^CCGGA
2549 TCCGGA
MvnI 2755 CGCG CG^CG
2935 CGCG
NcoI 2198 CCATGG C^CATGG
2340 CCATGG
PstI 2669 CTGCAG CTGCA^G
3016 CTGCAG
SmaI 2289 CCCGGG CCC^GGG
2738 CCCGGG
SmlI 868 CTCAAG C^T[CT][GA]AG
1129 CTTGAG
SspBI 213 TGTACA T^GTACA
1577 TGTACA
XmaCI 2289 CCCGGG C^CCGGG
2738 CCCGGG
XmaI 2289 CCCGGG C^CCGGG
2738 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DrdI DsaI
EaeI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII