(Circular) (Six-base) MAPSORT of: pFN21AB0207.seq Check: 5477 from: 1 to: 7774 pFN21AB0207 7774 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4765 4765 Size: 7774 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5058 5584 278 Size: 53 83 186 936 3522 526 2468 Fragments arranged by size: 3522 2468 936 526 186 83 53 AccI GT'mk_AC Cuts at: 1937 5117 1937 Size: 3180 4594 Acc65I G'GTAC_C Cuts at: 5095 5095 Size: 7774 AclI AA'CG_TT Cuts at: 1563 5901 6274 1563 Size: 4338 373 3063 Fragments arranged by size: 4338 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3219 4019 4850 5837 7221 7489 1632 Size: 1587 800 831 987 1384 268 1917 Fragments arranged by size: 1917 1587 1384 987 831 800 268 AfeI AGC'GCT Cuts at: 2834 2834 Size: 7774 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7577 AflIII A'CryG_T Cuts at: 1203 3459 6673 7650 1203 Size: 2256 3214 977 1327 Fragments arranged by size: 3214 2256 1327 977 AgeI A'CCGG_T Cuts at: 6605 6605 Size: 7774 AhdI GACnn_n'nnGTC Cuts at: 6503 6503 Size: 7774 AleI CACnn'nnGTG Cuts at: 1166 3392 1166 Size: 2226 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3252 4349 1184 Size: 2068 1097 4609 Fragments arranged by size: 4609 2068 1097 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2657 2689 2657 Size: 32 7742 AlwNI CAG_nnn'CTG Cuts at: 2341 4543 4979 5517 7089 2341 Size: 2202 436 538 1572 3026 Fragments arranged by size: 3026 2202 1572 538 436 ApaLI G'TGCA_C Cuts at: 3317 5831 6987 3317 Size: 2514 1156 4104 Fragments arranged by size: 4104 2514 1156 ApoI r'AATT_y Cuts at: 1493 3678 5083 5309 5461 7570 1493 Size: 2185 1405 226 152 2109 1697 Fragments arranged by size: 2185 2109 1697 1405 226 152 AseI AT'TA_AT Cuts at: 160 1032 6328 160 Size: 872 5296 1606 Fragments arranged by size: 5296 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7774 AvaI C'yCGr_G Cuts at: 1870 1943 3399 3443 4294 4601 4743 5099 1870 Size: 73 1456 44 851 307 142 356 4545 Fragments arranged by size: 4545 1456 851 356 307 142 73 44 BamHI G'GATC_C Cuts at: 2703 5104 5471 2703 Size: 2401 367 5006 Fragments arranged by size: 5006 2401 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2427 3311 4869 5095 6550 Size: 325 217 627 640 884 1558 226 1455 Cuts at: 6550 618 Size: 1842 Fragments arranged by size: 1842 1558 1455 884 640 627 325 226 217 BanII G_rGCy'C Cuts at: 1391 2148 2266 3035 3561 3967 5093 1391 Size: 757 118 769 526 406 1126 4072 Fragments arranged by size: 4072 1126 769 757 526 406 118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2551 3929 5009 5050 928 Size: 971 652 1378 1080 41 3652 Fragments arranged by size: 3652 1378 1080 971 652 41 BbvCI CC'TCA_GC Cuts at: 3281 3281 Size: 7774 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5965 5999 1903 Size: 34 4028 34 3678 Fragments arranged by size: 4028 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5666 6876 5666 Size: 1210 6564 BclI T'GATC_A Cuts at: 1548 2919 1548 Size: 1371 6403 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6385 136 Size: 107 122 71 631 605 4713 1525 Fragments arranged by size: 4713 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2988 3696 3719 3735 7769 2988 Size: 708 23 16 4034 2993 Fragments arranged by size: 4034 2993 708 23 16 BlpI GC'TnA_GC Cuts at: 2142 3627 3908 4022 4857 5191 2142 Size: 1485 281 114 835 334 4725 Fragments arranged by size: 4725 1485 835 334 281 114 Bme1580I G_kGCm'C Cuts at: 1790 2276 2430 2490 3321 4049 5835 6991 1790 Size: 486 154 60 831 728 1786 1156 2573 Fragments arranged by size: 2573 1786 1156 831 728 486 154 60 BmgBI CAC'GTC Cuts at: 1318 3394 3505 4036 1318 Size: 2076 111 531 5056 Fragments arranged by size: 5056 2076 531 111 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3970 4261 5505 Size: 416 53 83 399 24 2553 291 1244 Cuts at: 5505 6463 442 Size: 958 1753 Fragments arranged by size: 2553 1753 1244 958 416 399 291 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7774 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2654 2708 3035 4968 6434 1239 Size: 1415 54 327 1933 1466 2579 Fragments arranged by size: 2579 1933 1466 1415 327 54 Bpu10I CC'TnA_GC Cuts at: 2300 3281 2300 Size: 981 6793 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2858 3155 5899 6764 7062 2858 Size: 297 2744 865 298 3570 Fragments arranged by size: 3570 2744 865 298 297 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2714 3263 6437 882 Size: 617 1215 549 3174 2219 Fragments arranged by size: 3174 2219 1215 617 549 BsaAI yAC'GTr Cuts at: 493 1204 2917 3322 3427 3460 4525 4909 5565 Size: 711 1713 405 105 33 1065 384 656 Cuts at: 5565 493 Size: 2702 Fragments arranged by size: 2702 1713 1065 711 656 405 384 105 33 BsaBI GATnn'nnATC Cuts at: 2469 7768 2469 Size: 5299 2475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4534 5055 5581 5963 Size: 53 83 186 936 3001 521 526 382 Cuts at: 5963 6598 275 Size: 635 1451 Fragments arranged by size: 3001 1451 936 635 526 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3035 3948 4554 6207 6605 6879 7026 7437 3035 Size: 913 606 1653 398 274 147 411 3372 Fragments arranged by size: 3372 1653 913 606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 2657 2687 1178 Size: 30 165 30 768 30 456 30 6265 Fragments arranged by size: 6265 768 456 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2470 2765 2813 3364 3499 3686 4892 Size: 1009 275 295 48 551 135 187 1206 Cuts at: 4892 1186 Size: 4068 Fragments arranged by size: 4068 1206 1009 551 295 275 187 135 48 BseYI C'CCAG_C Cuts at: 4180 4192 4972 6977 7473 4180 Size: 12 780 2005 496 4481 Fragments arranged by size: 4481 2005 780 496 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2112 2414 4699 4859 1163 Size: 573 376 302 2285 160 4078 Fragments arranged by size: 4078 2285 573 376 302 160 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3477 4966 5224 5985 7013 1540 Size: 275 186 1476 1489 258 761 1028 2301 Fragments arranged by size: 2301 1489 1476 1028 761 275 258 186 BsiHKAI G_wGCw'C Cuts at: 3321 3491 4074 4884 5835 5920 6991 3321 Size: 170 583 810 951 85 1071 4104 Fragments arranged by size: 4104 1071 951 810 583 170 85 BsmI GAATG_Cn' Cuts at: 2260 3415 5285 5378 2260 Size: 1155 1870 93 4656 Fragments arranged by size: 4656 1870 1155 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2266 2276 2430 2490 3035 3321 Size: 399 358 118 10 154 60 545 286 Cuts at: 3321 3491 3561 3967 4049 4074 4884 5093 5835 Size: 170 70 406 82 25 810 209 742 Cuts at: 5835 5920 6991 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 810 742 545 406 399 358 286 209 170 154 118 85 82 70 60 25 10 BspEI T'CCGG_A Cuts at: 3035 4554 7437 3035 Size: 1519 2883 3372 Fragments arranged by size: 3372 2883 1519 BspHI T'CATG_A Cuts at: 5663 7393 5663 Size: 1730 6044 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3297 4109 4765 5129 844 Size: 2453 812 656 364 3489 Fragments arranged by size: 3489 2453 812 656 364 BsrBI CCG'CTC Cuts at: 1394 1823 3372 3577 4026 4042 4393 5661 1394 Size: 429 1549 205 449 16 351 1268 3507 Fragments arranged by size: 3507 1549 1268 449 429 351 205 16 BsrDI GCAATG_nn' Cuts at: 65 1248 6269 6443 65 Size: 1183 5021 174 1396 Fragments arranged by size: 5021 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3528 4718 4961 4991 6418 6605 1700 Size: 110 1718 1190 243 30 1427 187 2869 Fragments arranged by size: 2869 1718 1427 1190 243 187 110 30 BsrGI T'GTAC_A Cuts at: 96 2218 3582 96 Size: 2122 1364 4288 Fragments arranged by size: 4288 2122 1364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7774 BssSI C'ACGA_G Cuts at: 2372 3927 5834 6846 2372 Size: 1555 1907 1012 3300 Fragments arranged by size: 3300 1907 1555 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2135 3813 794 Size: 1341 1678 4755 Fragments arranged by size: 4755 1678 1341 BstBI TT'CG_AA Cuts at: 2168 6578 2168 Size: 4410 3364 BstEII G'GTnAC_C Cuts at: 1184 3437 1184 Size: 2253 5521 BstXI CCAn_nnnn'nTGG Cuts at: 2657 3394 2657 Size: 737 7037 BstYI r'GATC_y Cuts at: 1972 2703 2988 3591 3696 3719 3735 3915 4539 Size: 731 285 603 105 23 16 180 624 Cuts at: 4539 4610 5104 5471 5856 5873 7325 7411 7423 Size: 71 494 367 385 17 1452 86 12 Cuts at: 7423 7769 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 731 624 603 494 385 367 346 285 180 105 86 71 23 17 16 12 BtgI C'CryG_G Cuts at: 513 1065 3150 3822 4203 4345 513 Size: 552 2085 672 381 142 3942 Fragments arranged by size: 3942 2085 672 552 381 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7035 Fragments arranged by size: 7035 683 56 BtsI GCAGTG_nn' Cuts at: 2274 2397 2699 5290 6084 6104 2274 Size: 123 302 2591 794 20 3944 Fragments arranged by size: 3944 2591 794 302 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7774 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2776 2811 4289 4324 529 Size: 35 2212 35 1478 35 3979 Fragments arranged by size: 3979 2212 1478 35 35 35 DraI TTT'AAA Cuts at: 5078 5078 Size: 7774 DraIII CAC_nnn'GTG Cuts at: 4069 4069 Size: 7774 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3509 4424 5054 6781 809 Size: 507 2193 915 630 1727 1802 Fragments arranged by size: 2193 1802 1727 915 630 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2769 3117 3250 Size: 54 1425 50 275 18 939 348 133 Cuts at: 3250 3474 3538 4027 4206 4779 4963 5221 6110 Size: 224 64 489 179 573 184 258 889 Cuts at: 6110 7739 8 Size: 1629 43 Fragments arranged by size: 1629 1425 939 889 573 489 348 275 258 224 184 179 133 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3474 4963 5221 1537 Size: 275 1662 1489 258 4090 Fragments arranged by size: 4090 1662 1489 275 258 EarI CTCTTCn'nnn_ Cuts at: 1353 4715 5704 1353 Size: 3362 989 3423 Fragments arranged by size: 3423 3362 989 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 6361 6735 6881 387 Size: 1064 138 2780 1992 374 146 1280 Fragments arranged by size: 2780 1992 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2654 2708 3035 3219 4019 4850 4968 Size: 393 1022 54 327 184 800 831 118 Cuts at: 4968 5837 6434 7221 7489 1239 Size: 869 597 787 268 1524 Fragments arranged by size: 1524 1022 869 831 800 787 597 393 327 268 184 118 54 EcoNI CCTnn'n_nnAGG Cuts at: 2552 4001 4829 2552 Size: 1449 828 5497 Fragments arranged by size: 5497 1449 828 EcoO109I rG'GnC_Cy Cuts at: 4135 4969 4135 Size: 834 6940 EcoRI G'AATT_C Cuts at: 5083 5461 5083 Size: 378 7396 FspI TGC'GCA Cuts at: 6280 6280 Size: 7774 HaeII r_GCGC'y Cuts at: 1678 2836 5029 6921 1678 Size: 1158 2193 1892 2531 Fragments arranged by size: 2531 2193 1892 1158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2486 2494 2518 2611 Size: 32 135 32 890 24 8 24 93 Cuts at: 2611 2643 2980 3012 3017 3049 3278 3310 4524 Size: 32 337 32 5 32 229 32 1214 Cuts at: 4524 4556 4595 4627 4787 4819 6415 6447 6489 Size: 32 39 32 160 32 1596 32 42 Cuts at: 6489 6521 1373 Size: 32 2626 Fragments arranged by size: 2626 1596 1214 890 337 229 160 135 93 42 39 32 32 32 32 32 32 32 32 32 32 32 24 24 8 5 HincII GTy'rAC Cuts at: 669 1657 1938 3331 3655 4495 5118 5364 669 Size: 988 281 1393 324 840 623 246 3079 Fragments arranged by size: 3079 1393 988 840 623 324 281 246 HindIII A'AGCT_T Cuts at: 748 3708 748 Size: 2960 4814 HpaI GTT'AAC Cuts at: 5364 5364 Size: 7774 KpnI G_GTAC'C Cuts at: 5099 5099 Size: 7774 MfeI C'AATT_G Cuts at: 4480 5373 4480 Size: 893 6881 MluI A'CGCG_T Cuts at: 7650 7650 Size: 7774 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6888 7072 1305 Size: 5583 184 2007 Fragments arranged by size: 5583 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2771 3119 3252 4208 4781 Size: 54 1425 343 939 348 133 956 573 Cuts at: 4781 10 Size: 3003 Fragments arranged by size: 3003 1425 956 939 573 348 343 133 54 MslI CAynn'nnrTG Cuts at: 518 1166 2927 3234 3392 3458 4109 5732 6091 Size: 648 1761 307 158 66 651 1623 359 Cuts at: 6091 6250 518 Size: 159 2042 Fragments arranged by size: 2042 1761 1623 651 648 359 307 159 158 66 MspA1I CmG'CkG Cuts at: 1606 2443 2492 2812 3023 3143 4651 4801 4976 Size: 837 49 320 211 120 1508 150 175 Cuts at: 4976 5190 5867 7015 7260 1606 Size: 214 677 1148 245 2120 Fragments arranged by size: 2120 1508 1148 837 677 320 245 214 211 175 150 120 49 NaeI GCC'GGC Cuts at: 4963 4963 Size: 7774 NcoI C'CATG_G Cuts at: 513 1065 4203 4345 513 Size: 552 3138 142 3942 Fragments arranged by size: 3942 3138 552 142 NdeI CA'TA_TG Cuts at: 387 387 Size: 7774 NgoMIV G'CCGG_C Cuts at: 4961 4961 Size: 7774 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7774 NotI GC'GGCC_GC Cuts at: 5221 5221 Size: 7774 NspI r_CATG'y Cuts at: 1132 1229 2932 3064 3811 5132 6677 1132 Size: 97 1703 132 747 1321 1545 2229 Fragments arranged by size: 2229 1703 1545 1321 747 132 97 PasI CC'CwG_GG Cuts at: 1348 2452 2726 1348 Size: 1104 274 6396 Fragments arranged by size: 6396 1104 274 PciI A'CATG_T Cuts at: 6673 6673 Size: 7774 PflMI CCAn_nnn'nTGG Cuts at: 1108 4036 4087 4351 4667 1108 Size: 2928 51 264 316 4215 Fragments arranged by size: 4215 2928 316 264 51 PfoI T'CCnGG_A Cuts at: 4633 5031 4633 Size: 398 7376 PmeI GTTT'AAAC Cuts at: 5078 5078 Size: 7774 PmlI CAC'GTG Cuts at: 3322 3427 4525 3322 Size: 105 1098 6571 Fragments arranged by size: 6571 1098 105 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2657 2689 3548 3580 5843 5875 7356 7388 2657 Size: 32 859 32 2263 32 1481 32 3043 Fragments arranged by size: 3043 2263 1481 859 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4969 4969 Size: 7774 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7774 PsiI TTA'TAA Cuts at: 5344 5344 Size: 7774 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4880 4912 4880 Size: 32 7742 PstI C_TGCA'G Cuts at: 830 4678 5025 5126 830 Size: 3848 347 101 3478 Fragments arranged by size: 3848 3478 347 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7774 PvuII CAG'CTG Cuts at: 2443 3023 4801 2443 Size: 580 1778 5416 Fragments arranged by size: 5416 1778 580 SalI G'TCGA_C Cuts at: 1936 5116 1936 Size: 3180 4594 SanDI GG'GwC_CC Cuts at: 4969 4969 Size: 7774 SbfI CC_TGCA'GG Cuts at: 5126 5126 Size: 7774 ScaI AGT'ACT Cuts at: 6022 7734 6022 Size: 1712 6062 SexAI A'CCwGG_T Cuts at: 2789 2789 Size: 7774 SfcI C'TryA_G Cuts at: 826 1045 2807 4674 5021 5122 6257 6938 7129 Size: 219 1762 1867 347 101 1135 681 191 Cuts at: 7129 826 Size: 1471 Fragments arranged by size: 1867 1762 1471 1135 681 347 219 191 101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7774 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7774 SmaI CCC'GGG Cuts at: 1872 4296 4745 5101 1872 Size: 2424 449 356 4545 Fragments arranged by size: 4545 2424 449 356 SmlI C'TyrA_G Cuts at: 820 1017 1943 2873 3134 5878 6779 7041 820 Size: 197 926 930 261 2744 901 262 1553 Fragments arranged by size: 2744 1553 930 926 901 262 261 197 SnaBI TAC'GTA Cuts at: 493 5565 493 Size: 5072 2702 SpeI A'CTAG_T Cuts at: 152 2162 152 Size: 2010 5764 SphI G_CATG'C Cuts at: 1132 2932 5132 1132 Size: 1800 2200 3774 Fragments arranged by size: 3774 2200 1800 SspI AAT'ATT Cuts at: 5 52 5698 5 Size: 47 5646 2081 Fragments arranged by size: 5646 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7774 StyI C'CwwG_G Cuts at: 513 1065 3111 4203 4345 4891 5213 513 Size: 552 2046 1092 142 546 322 3074 Fragments arranged by size: 3074 2046 1092 552 546 322 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4300 5801 5818 5971 6156 6601 325 Size: 1255 2720 1501 17 153 185 445 1498 Fragments arranged by size: 2720 1501 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 2218 3582 Size: 274 80 33 51 1443 210 31 1364 Cuts at: 3582 3975 6020 7732 96 Size: 393 2045 1712 138 Fragments arranged by size: 2045 1712 1443 1364 393 274 210 138 80 51 33 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3067 3099 7734 7766 3067 Size: 32 4635 32 3075 Fragments arranged by size: 4635 3075 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2555 1316 Size: 1239 6535 XbaI T'CTAG_A Cuts at: 2842 5110 2842 Size: 2268 5506 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4858 4858 Size: 7774 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7774 XmaI C'CCGG_G Cuts at: 1870 4294 4743 5099 1870 Size: 2424 449 356 4545 Fragments arranged by size: 4545 2424 449 356 XmnI GAAnn'nnTTC Cuts at: 5903 5903 Size: 7774 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5056 5582 276 Size: 53 83 186 936 3522 526 2468 Fragments arranged by size: 3522 2468 936 526 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI