Restriction map for
pFN21AB0207
(Linear) (Six-base) MAPSORT of: KIBB0207.seq Check: 3825 from: 1 to: 3069
KIBB0207.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2760 3069
Size: 2760 309
AatII G_ACGT'C
Cuts at: 0 3053 3069
Size: 3053 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1214 2014 2845 3069
Size: 1214 800 831 224
Fragments arranged by size:
1214 831 800 224
AfeI AGC'GCT
Cuts at: 0 829 3069
Size: 829 2240
AflIII A'CryG_T
Cuts at: 0 1454 3069
Size: 1454 1615
AleI CACnn'nnGTG
Cuts at: 0 1387 3069
Size: 1387 1682
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1247 2344 3069
Size: 1247 1097 725
Fragments arranged by size:
1247 1097 725
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 652 684 3069
Size: 652 32 2385
Fragments arranged by size:
2385 652 32
AlwNI CAG_nnn'CTG
Cuts at: 0 336 2538 2974 3069
Size: 336 2202 436 95
Fragments arranged by size:
2202 436 336 95
ApaLI G'TGCA_C
Cuts at: 0 1312 3069
Size: 1312 1757
ApoI r'AATT_y
Cuts at: 0 1673 3069
Size: 1673 1396
AvaI C'yCGr_G
Cuts at: 0 1394 1438 2289 2596 2738 3069
Size: 1394 44 851 307 142 331
Fragments arranged by size:
1394 851 331 307 142 44
BamHI G'GATC_C
Cuts at: 0 698 3069
Size: 698 2371
BanI G'GyrC_C
Cuts at: 0 422 1306 2864 3069
Size: 422 884 1558 205
Fragments arranged by size:
1558 884 422 205
BanII G_rGCy'C
Cuts at: 0 143 261 1030 1556 1962 3069
Size: 143 118 769 526 406 1107
Fragments arranged by size:
1107 769 526 406 143 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 546 1924 3004 3045 3069
Size: 546 1378 1080 41 24
Fragments arranged by size:
1378 1080 546 41 24
BbvCI CC'TCA_GC
Cuts at: 0 1276 3069
Size: 1276 1793
BclI T'GATC_A
Cuts at: 0 914 3069
Size: 914 2155
BglII A'GATC_T
Cuts at: 0 983 1691 1714 1730 3069
Size: 983 708 23 16 1339
Fragments arranged by size:
1339 983 708 23 16
BlpI GC'TnA_GC
Cuts at: 0 137 1622 1903 2017 2852 3069
Size: 137 1485 281 114 835 217
Fragments arranged by size:
1485 835 281 217 137 114
Bme1580I G_kGCm'C
Cuts at: 0 271 425 485 1316 2044 3069
Size: 271 154 60 831 728 1025
Fragments arranged by size:
1025 831 728 271 154 60
BmgBI CAC'GTC
Cuts at: 0 1389 1500 2031 3069
Size: 1389 111 531 1038
Fragments arranged by size:
1389 1038 531 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 1965 2256 3069
Size: 1965 291 813
Fragments arranged by size:
1965 813 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 703 1030 2963 3069
Size: 649 54 327 1933 106
Fragments arranged by size:
1933 649 327 106 54
Bpu10I CC'TnA_GC
Cuts at: 0 295 1276 3069
Size: 295 981 1793
Fragments arranged by size:
1793 981 295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1150 3069
Size: 853 297 1919
Fragments arranged by size:
1919 853 297
BsaI GGTCTCn'nnnn_
Cuts at: 0 709 1258 3069
Size: 709 549 1811
Fragments arranged by size:
1811 709 549
BsaAI yAC'GTr
Cuts at: 0 912 1317 1422 1455 2520 2904 3069
Size: 912 405 105 33 1065 384 165
Fragments arranged by size:
1065 912 405 384 165 105 33
BsaBI GATnn'nnATC
Cuts at: 0 464 3069
Size: 464 2605
BsaHI Gr'CG_yC
Cuts at: 0 2529 3050 3069
Size: 2529 521 19
Fragments arranged by size:
2529 521 19
BsaWI w'CCGG_w
Cuts at: 0 1030 1943 2549 3069
Size: 1030 913 606 520
Fragments arranged by size:
1030 913 606 520
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 652 682 3069
Size: 166 30 456 30 2387
Fragments arranged by size:
2387 456 166 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 465 760 808 1359 1494 1681 2887
Size: 190 275 295 48 551 135 187 1206
Cuts at: 2887 3069
Size: 182
Fragments arranged by size:
1206 551 295 275 190 187 182 135
48
BseYI C'CCAG_C
Cuts at: 0 2175 2187 2967 3069
Size: 2175 12 780 102
Fragments arranged by size:
2175 780 102 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 409 2694 2854 3069
Size: 107 302 2285 160 215
Fragments arranged by size:
2285 302 215 160 107
BsiEI CG_ry'CG
Cuts at: 0 1472 2961 3069
Size: 1472 1489 108
Fragments arranged by size:
1489 1472 108
BsiHKAI G_wGCw'C
Cuts at: 0 1316 1486 2069 2879 3069
Size: 1316 170 583 810 190
Fragments arranged by size:
1316 810 583 190 170
BsmI GAATG_Cn'
Cuts at: 0 255 1410 3069
Size: 255 1155 1659
Fragments arranged by size:
1659 1155 255
Bsp1286I G_dGCh'C
Cuts at: 0 143 261 271 425 485 1030 1316 1486
Size: 143 118 10 154 60 545 286 170
Cuts at: 1486 1556 1962 2044 2069 2879 3069
Size: 70 406 82 25 810 190
Fragments arranged by size:
810 545 406 286 190 170 154 143
118 82 70 60 25 10
BspEI T'CCGG_A
Cuts at: 0 1030 2549 3069
Size: 1030 1519 520
Fragments arranged by size:
1519 1030 520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1292 2104 2760 3069
Size: 1292 812 656 309
Fragments arranged by size:
1292 812 656 309
BsrBI CCG'CTC
Cuts at: 0 1367 1572 2021 2037 2388 3069
Size: 1367 205 449 16 351 681
Fragments arranged by size:
1367 681 449 351 205 16
BsrFI r'CCGG_y
Cuts at: 0 1523 2713 2956 2986 3069
Size: 1523 1190 243 30 83
Fragments arranged by size:
1523 1190 243 83 30
BsrGI T'GTAC_A
Cuts at: 0 213 1577 3069
Size: 213 1364 1492
Fragments arranged by size:
1492 1364 213
BssSI C'ACGA_G
Cuts at: 0 367 1922 3069
Size: 367 1555 1147
Fragments arranged by size:
1555 1147 367
BstAPI GCAn_nnn'nTGC
Cuts at: 0 130 1808 3069
Size: 130 1678 1261
Fragments arranged by size:
1678 1261 130
BstBI TT'CG_AA
Cuts at: 0 163 3069
Size: 163 2906
BstEII G'GTnAC_C
Cuts at: 0 1432 3069
Size: 1432 1637
BstXI CCAn_nnnn'nTGG
Cuts at: 0 652 1389 3069
Size: 652 737 1680
Fragments arranged by size:
1680 737 652
BstYI r'GATC_y
Cuts at: 0 698 983 1586 1691 1714 1730 1910 2534
Size: 698 285 603 105 23 16 180 624
Cuts at: 2534 2605 3069
Size: 71 464
Fragments arranged by size:
698 624 603 464 285 180 105 71
23 16
BtgI C'CryG_G
Cuts at: 0 1145 1817 2198 2340 3069
Size: 1145 672 381 142 729
Fragments arranged by size:
1145 729 672 381 142
BtsI GCAGTG_nn'
Cuts at: 0 269 392 694 3069
Size: 269 123 302 2375
Fragments arranged by size:
2375 302 269 123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 771 806 2284 2319 3069
Size: 771 35 1478 35 750
Fragments arranged by size:
1478 771 750 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2064 3069
Size: 2064 1005
DrdI GACnn_nn'nnGTC
Cuts at: 0 1504 2419 3049 3069
Size: 1504 915 630 20
Fragments arranged by size:
1504 915 630 20
EaeI y'GGCC_r
Cuts at: 0 764 1112 1245 1469 1533 2022 2201 2774
Size: 764 348 133 224 64 489 179 573
Cuts at: 2774 2958 3069
Size: 184 111
Fragments arranged by size:
764 573 489 348 224 184 179 133
111 64
EagI C'GGCC_G
Cuts at: 0 1469 2958 3069
Size: 1469 1489 111
Fragments arranged by size:
1489 1469 111
EarI CTCTTCn'nnn_
Cuts at: 0 2710 3069
Size: 2710 359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2364 3069
Size: 2364 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 703 1030 1214 2014 2845 2963 3069
Size: 649 54 327 184 800 831 118 106
Fragments arranged by size:
831 800 649 327 184 118 106 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 1996 2824 3069
Size: 547 1449 828 245
Fragments arranged by size:
1449 828 547 245
EcoO109I rG'GnC_Cy
Cuts at: 0 2130 2964 3069
Size: 2130 834 105
Fragments arranged by size:
2130 834 105
HaeII r_GCGC'y
Cuts at: 0 831 3024 3069
Size: 831 2193 45
Fragments arranged by size:
2193 831 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 481 489 513 606 638 975 1007
Size: 457 24 8 24 93 32 337 32
Cuts at: 1007 1012 1044 1273 1305 2519 2551 2590 2622
Size: 5 32 229 32 1214 32 39 32
Cuts at: 2622 2782 2814 3069
Size: 160 32 255
Fragments arranged by size:
1214 457 337 255 229 160 93 39
32 32 32 32 32 32 32 24
24 8 5
HincII GTy'rAC
Cuts at: 0 1326 1650 2490 3069
Size: 1326 324 840 579
Fragments arranged by size:
1326 840 579 324
HindIII A'AGCT_T
Cuts at: 0 1703 3069
Size: 1703 1366
MfeI C'AATT_G
Cuts at: 0 2475 3069
Size: 2475 594
MscI TGG'CCA
Cuts at: 0 766 1114 1247 2203 2776 3069
Size: 766 348 133 956 573 293
Fragments arranged by size:
956 766 573 348 293 133
MslI CAynn'nnrTG
Cuts at: 0 922 1229 1387 1453 2104 3069
Size: 922 307 158 66 651 965
Fragments arranged by size:
965 922 651 307 158 66
MspA1I CmG'CkG
Cuts at: 0 438 487 807 1018 1138 2646 2796 2971
Size: 438 49 320 211 120 1508 150 175
Cuts at: 2971 3069
Size: 98
Fragments arranged by size:
1508 438 320 211 175 150 120 98
49
NaeI GCC'GGC
Cuts at: 0 2958 3069
Size: 2958 111
NcoI C'CATG_G
Cuts at: 0 2198 2340 3069
Size: 2198 142 729
Fragments arranged by size:
2198 729 142
NgoMIV G'CCGG_C
Cuts at: 0 2956 3069
Size: 2956 113
NspI r_CATG'y
Cuts at: 0 927 1059 1806 3069
Size: 927 132 747 1263
Fragments arranged by size:
1263 927 747 132
PasI CC'CwG_GG
Cuts at: 0 447 721 3069
Size: 447 274 2348
Fragments arranged by size:
2348 447 274
PflMI CCAn_nnn'nTGG
Cuts at: 0 2031 2082 2346 2662 3069
Size: 2031 51 264 316 407
Fragments arranged by size:
2031 407 316 264 51
PfoI T'CCnGG_A
Cuts at: 0 2628 3026 3069
Size: 2628 398 43
Fragments arranged by size:
2628 398 43
PmlI CAC'GTG
Cuts at: 0 1317 1422 2520 3069
Size: 1317 105 1098 549
Fragments arranged by size:
1317 1098 549 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 652 684 1543 1575 3069
Size: 652 32 859 32 1494
Fragments arranged by size:
1494 859 652 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2964 3069
Size: 2964 105
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2875 2907 3069
Size: 2875 32 162
Fragments arranged by size:
2875 162 32
PstI C_TGCA'G
Cuts at: 0 2673 3020 3069
Size: 2673 347 49
Fragments arranged by size:
2673 347 49
PvuII CAG'CTG
Cuts at: 0 438 1018 2796 3069
Size: 438 580 1778 273
Fragments arranged by size:
1778 580 438 273
SanDI GG'GwC_CC
Cuts at: 0 2964 3069
Size: 2964 105
SexAI A'CCwGG_T
Cuts at: 0 784 3069
Size: 784 2285
SfcI C'TryA_G
Cuts at: 0 802 2669 3016 3069
Size: 802 1867 347 53
Fragments arranged by size:
1867 802 347 53
SmaI CCC'GGG
Cuts at: 0 2291 2740 3069
Size: 2291 449 329
Fragments arranged by size:
2291 449 329
SmlI C'TyrA_G
Cuts at: 0 868 1129 3069
Size: 868 261 1940
Fragments arranged by size:
1940 868 261
SpeI A'CTAG_T
Cuts at: 0 157 3069
Size: 157 2912
SphI G_CATG'C
Cuts at: 0 927 3069
Size: 927 2142
StyI C'CwwG_G
Cuts at: 0 1106 2198 2340 2886 3069
Size: 1106 1092 142 546 183
Fragments arranged by size:
1106 1092 546 183 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2295 3069
Size: 2295 774
TatI w'GTAC_w
Cuts at: 0 182 213 1577 1970 3069
Size: 182 31 1364 393 1099
Fragments arranged by size:
1364 1099 393 182 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1062 1094 3069
Size: 1062 32 1975
Fragments arranged by size:
1975 1062 32
Tth111I GACn'n_nGTC
Cuts at: 0 550 3069
Size: 550 2519
XbaI T'CTAG_A
Cuts at: 0 837 3069
Size: 837 2232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2853 3069
Size: 2853 216
XmaI C'CCGG_G
Cuts at: 0 2289 2738 3069
Size: 2289 449 331
Fragments arranged by size:
2289 449 331
ZraI GAC'GTC
Cuts at: 0 3051 3069
Size: 3051 18
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BclI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII MfeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI
PsrI PstI PvuII SanDI SexAI SfcI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI ApaI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI
BglI BmtI BplI BsiWI BsmBI BspHI BsrDI BssHII
BstZ17I Bsu36I BtgZI ClaI DraI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MluI
MmeI NarI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI