Restriction map for
pFN21AB0150
(Linear) (Six-base) MAPSORT of: KIBB0150.seq Check: 3447 from: 1 to: 4392
KIBB0150.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 2029 4392
Size: 2029 2363
AclI AA'CG_TT
Cuts at: 0 404 4392
Size: 404 3988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1594 1799 2989 3562 4392
Size: 1594 205 1190 573 830
Fragments arranged by size:
1594 1190 830 573 205
AhdI GACnn_n'nnGTC
Cuts at: 0 3135 4392
Size: 3135 1257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2659 4392
Size: 2659 1733
AlwNI CAG_nnn'CTG
Cuts at: 0 271 1221 1932 2170 2323 3702 4392
Size: 271 950 711 238 153 1379 690
Fragments arranged by size:
1379 950 711 690 271 238 153
ApoI r'AATT_y
Cuts at: 0 165 236 508 1269 2063 2591 2655 2790
Size: 165 71 272 761 794 528 64 135
Cuts at: 2790 3264 4392
Size: 474 1128
Fragments arranged by size:
1128 794 761 528 474 272 165 135
71 64
AseI AT'TA_AT
Cuts at: 0 2341 3689 4392
Size: 2341 1348 703
Fragments arranged by size:
2341 1348 703
AvaI C'yCGr_G
Cuts at: 0 4285 4392
Size: 4285 107
BanI G'GyrC_C
Cuts at: 0 2482 2862 3387 3681 4392
Size: 2482 380 525 294 711
Fragments arranged by size:
2482 711 525 380 294
BanII G_rGCy'C
Cuts at: 0 1480 1775 1961 2209 2574 3234 3970 4392
Size: 1480 295 186 248 365 660 736 422
Fragments arranged by size:
1480 736 660 422 365 295 248 186
BbeI G_GCGC'C
Cuts at: 0 2486 3391 4392
Size: 2486 905 1001
Fragments arranged by size:
2486 1001 905
BbvCI CC'TCA_GC
Cuts at: 0 1618 2744 4392
Size: 1618 1126 1648
Fragments arranged by size:
1648 1618 1126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 319 353 4392
Size: 319 34 4039
Fragments arranged by size:
4039 319 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 315 843 1980 4392
Size: 315 528 1137 2412
Fragments arranged by size:
2412 1137 528 315
BclI T'GATC_A
Cuts at: 0 1947 4392
Size: 1947 2445
BfrBI ATG'CAT
Cuts at: 0 3412 3756 4392
Size: 3412 344 636
Fragments arranged by size:
3412 636 344
BglI GCCn_nnn'nGGC
Cuts at: 0 1216 2987 4042 4392
Size: 1216 1771 1055 350
Fragments arranged by size:
1771 1216 1055 350
Bme1580I G_kGCm'C
Cuts at: 0 1631 2865 4392
Size: 1631 1234 1527
Fragments arranged by size:
1631 1527 1234
BmgBI CAC'GTC
Cuts at: 0 842 4392
Size: 842 3550
BmrI ACTGGGnnnn_n'
Cuts at: 0 1505 1690 2641 3956 4392
Size: 1505 185 951 1315 436
Fragments arranged by size:
1505 1315 951 436 185
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3881 4392
Size: 3881 511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 1502 1838 2873 3161 3298 3397 3686
Size: 18 1484 336 1035 288 137 99 289
Cuts at: 3686 4392
Size: 706
Fragments arranged by size:
1484 1035 706 336 289 288 137 99
18
Bpu10I CC'TnA_GC
Cuts at: 0 1618 1968 2143 2744 4392
Size: 1618 350 175 601 1648
Fragments arranged by size:
1648 1618 601 350 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 2032 2317 3336 4392
Size: 379 1653 285 1019 1056
Fragments arranged by size:
1653 1056 1019 379 285
BsaI GGTCTCn'nnnn_
Cuts at: 0 1641 4392
Size: 1641 2751
BsaHI Gr'CG_yC
Cuts at: 0 2483 3136 3388 4392
Size: 2483 653 252 1004
Fragments arranged by size:
2483 1004 653 252
BsaWI w'CCGG_w
Cuts at: 0 1149 4392
Size: 1149 3243
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 929 959 1984 2014 4392
Size: 929 30 1025 30 2378
Fragments arranged by size:
2378 1025 929 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 1361 2380 2434 3074 3295 4392
Size: 649 712 1019 54 640 221 1097
Fragments arranged by size:
1097 1019 712 649 640 221 54
BseYI C'CCAG_C
Cuts at: 0 82 342 884 2949 2974 4032 4103 4392
Size: 82 260 542 2065 25 1058 71 289
Fragments arranged by size:
2065 1058 542 289 260 82 71 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1028 1245 3565 4392
Size: 310 718 217 2320 827
Fragments arranged by size:
2320 827 718 310 217
BsiEI CG_ry'CG
Cuts at: 0 1859 4392
Size: 1859 2533
BsiHKAI G_wGCw'C
Cuts at: 0 2605 3234 4392
Size: 2605 629 1158
Fragments arranged by size:
2605 1158 629
BsmI GAATG_Cn'
Cuts at: 0 994 1539 2229 4392
Size: 994 545 690 2163
Fragments arranged by size:
2163 994 690 545
BsmBI CGTCTCn'nnnn_
Cuts at: 0 848 4392
Size: 848 3544
Bsp1286I G_dGCh'C
Cuts at: 0 1480 1631 1775 1961 2209 2574 2605 2865
Size: 1480 151 144 186 248 365 31 260
Cuts at: 2865 3234 3970 4392
Size: 369 736 422
Fragments arranged by size:
1480 736 422 369 365 260 248 186
151 144 31
BspEI T'CCGG_A
Cuts at: 0 1149 4392
Size: 1149 3243
BspHI T'CATG_A
Cuts at: 0 642 969 3017 4392
Size: 642 327 2048 1375
Fragments arranged by size:
2048 1375 642 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 530 1307 1856 2627 4281 4392
Size: 530 777 549 771 1654 111
Fragments arranged by size:
1654 777 771 549 530 111
BsrDI GCAATG_nn'
Cuts at: 0 2354 2674 3639 4392
Size: 2354 320 965 753
Fragments arranged by size:
2354 965 753 320
BsrFI r'CCGG_y
Cuts at: 0 2199 2918 4392
Size: 2199 719 1474
Fragments arranged by size:
2199 1474 719
BsrGI T'GTAC_A
Cuts at: 0 4148 4392
Size: 4148 244
BssSI C'ACGA_G
Cuts at: 0 2472 4392
Size: 2472 1920
BstAPI GCAn_nnn'nTGC
Cuts at: 0 271 4392
Size: 271 4121
BstEII G'GTnAC_C
Cuts at: 0 2386 4392
Size: 2386 2006
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4342 4392
Size: 4342 50
BstYI r'GATC_y
Cuts at: 0 550 822 1152 2319 3336 3456 4392
Size: 550 272 330 1167 1017 120 936
Fragments arranged by size:
1167 1017 936 550 330 272 120
Bsu36I CC'TnA_GG
Cuts at: 0 1373 3297 3526 4392
Size: 1373 1924 229 866
Fragments arranged by size:
1924 1373 866 229
BtgI C'CryG_G
Cuts at: 0 3103 3473 3533 4178 4277 4392
Size: 3103 370 60 645 99 115
Fragments arranged by size:
3103 645 370 115 99 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1453 3568 4392
Size: 1453 2115 824
Fragments arranged by size:
2115 1453 824
BtsI GCAGTG_nn'
Cuts at: 0 688 1233 1872 2406 2584 4392
Size: 688 545 639 534 178 1808
Fragments arranged by size:
1808 688 639 545 534 178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3809 3844 4176 4211 4392
Size: 3809 35 332 35 181
Fragments arranged by size:
3809 332 181 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 3006 4392
Size: 3006 1386
EaeI y'GGCC_r
Cuts at: 0 1856 2108 2920 2988 4392
Size: 1856 252 812 68 1404
Fragments arranged by size:
1856 1404 812 252 68
EagI C'GGCC_G
Cuts at: 0 1856 4392
Size: 1856 2536
EarI CTCTTCn'nnn_
Cuts at: 0 449 626 756 1048 4392
Size: 449 177 130 292 3344
Fragments arranged by size:
3344 449 292 177 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 591 4100 4392
Size: 591 3509 292
Fragments arranged by size:
3509 591 292
EcoICRI GAG'CTC
Cuts at: 0 3232 4392
Size: 3232 1160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 1502 1594 1799 1838 2873 2989 3161
Size: 18 1484 92 205 39 1035 116 172
Cuts at: 3161 3298 3397 3562 3686 4392
Size: 137 99 165 124 706
Fragments arranged by size:
1484 1035 706 205 172 165 137 124
116 99 92 39 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1621 2146 2747 3935 4262 4392
Size: 1621 525 601 1188 327 130
Fragments arranged by size:
1621 1188 601 525 327 130
EcoO109I rG'GnC_Cy
Cuts at: 0 346 363 891 1218 1614 3058 4044 4282
Size: 346 17 528 327 396 1444 986 238
Cuts at: 4282 4392
Size: 110
Fragments arranged by size:
1444 986 528 396 346 327 238 110
17
FspI TGC'GCA
Cuts at: 0 926 4392
Size: 926 3466
HaeII r_GCGC'y
Cuts at: 0 2486 3391 4392
Size: 2486 905 1001
Fragments arranged by size:
2486 1001 905
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 420 452 721 753 828 860
Size: 292 32 96 32 269 32 75 32
Cuts at: 860 951 983 2748 2780 3121 3153 3509 3541
Size: 91 32 1765 32 341 32 356 32
Cuts at: 3541 4392
Size: 851
Fragments arranged by size:
1765 851 356 341 292 269 96 91
75 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2117 2502 4392
Size: 2117 385 1890
Fragments arranged by size:
2117 1890 385
HindIII A'AGCT_T
Cuts at: 0 1533 1734 4392
Size: 1533 201 2658
Fragments arranged by size:
2658 1533 201
HpaI GTT'AAC
Cuts at: 0 2117 4392
Size: 2117 2275
KasI G'GCGC_C
Cuts at: 0 2482 3387 4392
Size: 2482 905 1005
Fragments arranged by size:
2482 1005 905
MfeI C'AATT_G
Cuts at: 0 594 4392
Size: 594 3798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1964 2547 3389 4076 4392
Size: 580 1384 583 842 687 316
Fragments arranged by size:
1384 842 687 583 580 316
MscI TGG'CCA
Cuts at: 0 2110 2990 4392
Size: 2110 880 1402
Fragments arranged by size:
2110 1402 880
MslI CAynn'nnrTG
Cuts at: 0 968 1450 2812 2965 4144 4171 4392
Size: 968 482 1362 153 1179 27 221
Fragments arranged by size:
1362 1179 968 482 221 153 27
MspA1I CmG'CkG
Cuts at: 0 271 1808 1929 2542 3208 3246 3483 4338
Size: 271 1537 121 613 666 38 237 855
Cuts at: 4338 4392
Size: 54
Fragments arranged by size:
1537 855 666 613 271 237 121 54
38
NarI GG'CG_CC
Cuts at: 0 2483 3388 4392
Size: 2483 905 1004
Fragments arranged by size:
2483 1004 905
NcoI C'CATG_G
Cuts at: 0 3103 3473 4178 4277 4392
Size: 3103 370 705 99 115
Fragments arranged by size:
3103 705 370 115 99
NdeI CA'TA_TG
Cuts at: 0 1112 4392
Size: 1112 3280
NsiI A_TGCA'T
Cuts at: 0 3414 3758 4392
Size: 3414 344 634
Fragments arranged by size:
3414 634 344
NspI r_CATG'y
Cuts at: 0 493 1308 1359 4098 4149 4392
Size: 493 815 51 2739 51 243
Fragments arranged by size:
2739 815 493 243 51 51
PasI CC'CwG_GG
Cuts at: 0 3432 4392
Size: 3432 960
PflMI CCAn_nnn'nTGG
Cuts at: 0 4123 4392
Size: 4123 269
PfoI T'CCnGG_A
Cuts at: 0 1434 1815 2169 3236 4392
Size: 1434 381 354 1067 1156
Fragments arranged by size:
1434 1156 1067 381 354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 4392
Size: 1393 32 2967
Fragments arranged by size:
2967 1393 32
PpuMI rG'GwC_Cy
Cuts at: 0 891 3058 4282 4392
Size: 891 2167 1224 110
Fragments arranged by size:
2167 1224 891 110
PstI C_TGCA'G
Cuts at: 0 1530 1928 3207 4392
Size: 1530 398 1279 1185
Fragments arranged by size:
1530 1279 1185 398
PvuII CAG'CTG
Cuts at: 0 271 1808 1929 2542 3208 3246 4338 4392
Size: 271 1537 121 613 666 38 1092 54
Fragments arranged by size:
1537 1092 666 613 271 121 54 38
SacI G_AGCT'C
Cuts at: 0 3234 4392
Size: 3234 1158
SanDI GG'GwC_CC
Cuts at: 0 4282 4392
Size: 4282 110
SapI GCTCTTCn'nnn_
Cuts at: 0 449 1048 4392
Size: 449 599 3344
Fragments arranged by size:
3344 599 449
SbfI CC_TGCA'GG
Cuts at: 0 1530 4392
Size: 1530 2862
ScaI AGT'ACT
Cuts at: 0 977 4392
Size: 977 3415
SexAI A'CCwGG_T
Cuts at: 0 893 4392
Size: 893 3499
SfcI C'TryA_G
Cuts at: 0 311 536 1526 1924 3125 3203 4392
Size: 311 225 990 398 1201 78 1189
Fragments arranged by size:
1201 1189 990 398 311 225 78
SfoI GGC'GCC
Cuts at: 0 2484 3389 4392
Size: 2484 905 1003
Fragments arranged by size:
2484 1003 905
SmlI C'TyrA_G
Cuts at: 0 358 2047 2296 3351 4392
Size: 358 1689 249 1055 1041
Fragments arranged by size:
1689 1055 1041 358 249
SphI G_CATG'C
Cuts at: 0 1308 1359 4392
Size: 1308 51 3033
Fragments arranged by size:
3033 1308 51
SspI AAT'ATT
Cuts at: 0 4387 4392
Size: 4387 5
StuI AGG'CCT
Cuts at: 0 3663 4392
Size: 3663 729
StyI C'CwwG_G
Cuts at: 0 828 1462 2784 2928 3103 3473 4178 4277
Size: 828 634 1322 144 175 370 705 99
Cuts at: 4277 4392
Size: 115
Fragments arranged by size:
1322 828 705 634 370 175 144 115
99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1524 4392
Size: 1524 2868
TatI w'GTAC_w
Cuts at: 0 975 4148 4392
Size: 975 3173 244
Fragments arranged by size:
3173 975 244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 929 961 2165 2197 2447 2479 4392
Size: 929 32 1204 32 250 32 1913
Fragments arranged by size:
1913 1204 929 250 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2855 3372 4392
Size: 2855 517 1020
Fragments arranged by size:
2855 1020 517
Enzymes that do cut:
AccI AclI AcuI AhdI AlfI AlwNI ApoI AseI
AvaI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI
MslI MspA1I NarI NcoI NdeI NsiI NspI PasI
PflMI PfoI PpiI PpuMI PstI PvuII SacI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SmlI SphI
SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbsI BglII BlpI BmtI BsaAI BsaBI BsiWI BsrBI
BssHII BstBI BstZ17I ClaI DraI DraIII EcoRI EcoRV
FalI FseI FspAI KpnI MluI NaeI NgoMIV NheI
NotI NruI PacI PciI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI