Restriction map for
pFN21AB0100
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BclI BfrI BglII
BlnI BsaBI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstYI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MflI MluI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccIII 1767 TCCGGA T^CCGGA
AflIII 1356 ACACGT A^C[GA][CT]GT
Alw44I 1480 GTGCAC G^TGCAC
ApaLI 1480 GTGCAC G^TGCAC
BbvCI 403 GCTGAGG CCTCAGC(-5/-2)
BfaI 2953 CTAG C^TAG
BseAI 1767 TCCGGA T^CCGGA
BsiEI 1847 CGGTCG CG[GA][CT]^CG
BsmBI 730 GAGACG CGTCTC(1/5)
BsmI 981 GAATGC GAATGC(1/-1)
BspEI 1767 TCCGGA T^CCGGA
BsrDI 2496 CATTGC GCAATG(2/0)
BsrFI 1696 GCCGGC [GA]^CCGG[CT]
BssSI 923 CACGAG CACGAG(-5/-1)
BstEII 2211 GGTCACC G^GT[ACGT]ACC
BstPI 2211 GGTCACC G^GT[ACGT]ACC
Cfr10I 1696 GCCGGC [GA]^CCGG[CT]
EcoO65I 2211 GGTCACC G^GT[ACGT]ACC
MaeI 2953 CTAG C^TAG
MroI 1767 TCCGGA T^CCGGA
MseI 1388 TTAA T^TAA
NaeI 1696 GCCGGC GCC^GGC
NgoMIV 1696 GCCGGC G^CCGGC
PflMI 2727 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1957 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1402 GAGCTC GAGCT^C
SbfI 2772 CCTGCAGG CCTGCA^GG
SexAI 1484 ACCAGGT A^CC[AT]GGT
SmaI 1985 CCCGGG CCC^GGG
SmlI 1742 CTCAAG C^T[CT][GA]AG
SphI 337 GCATGC GCATG^C
Sse8387I 2772 CCTGCAGG CCTGCA^GG
TfiI 1594 GAATC G^A[AT]TC
Tru9I 1388 TTAA T^TAA
Van91I 2727 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1985 CCCGGG C^CCGGG
XmaI 1985 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 292 CGCG CG^CG
619 CGCG
AlwNI 2104 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2350 CAGTACCTG
BbeI 1166 GGCGCC GGCGC^C
2122 GGCGCC
BciVI 910 GGATAC GTATCC(6/5)
2543 GGATAC
BsaAI 628 TACGTG [CT]AC^GT[GA]
1357 CACGTA
BsaI 376 GAGACC GGTCTC(1/5)
1621 GAGACC
BsaWI 118 ACCGGA [AT]^CCGG[AT]
1767 TCCGGA
BstUI 292 CGCG CG^CG
619 CGCG
EcoT14I 458 CCAAGG C^C[AT][AT]GG
1781 CCTTGG
HaeII 1166 GGCGCC [GA]GCGC^[CT]
2122 GGCGCC
HgaI 197 GACGC GACGC(5/10)
732 GACGC
KasI 1166 GGCGCC G^GCGCC
2122 GGCGCC
MfeI 261 CAATTG C^AATTG
470 CAATTG
MunI 261 CAATTG C^AATTG
470 CAATTG
MvnI 292 CGCG CG^CG
619 CGCG
NarI 1166 GGCGCC GG^CGCC
2122 GGCGCC
NspI 220 GCATGT [GA]CATG^[CT]
337 GCATGC
SapI 678 GCTCTTC GCTCTTC(1/4)
1203 GCTCTTC
SfoI 1166 GGCGCC GGC^GCC
2122 GGCGCC
StyI 458 CCAAGG C^C[AT][AT]GG
1781 CCTTGG
Tsp509I 262 AATT ^AATT
471 AATT
XcmI 154 CCACCAGGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1832 CCATCACCGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
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AciI AcyI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
StuI TaqI TseI Tsp45I TspRI
TthHB8I