Restriction map for
pFN21AB0100
(Linear) (Six-base) MAPSORT of: KIBB0100.seq Check: 1823 from: 1 to: 3063
KIBB0100.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 376 700 3063
Size: 376 324 2363
Fragments arranged by size:
2363 376 324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 65 2913 3010 3063
Size: 29 36 2848 97 53
Fragments arranged by size:
2848 97 53 36 29
AflIII A'CryG_T
Cuts at: 0 1356 3063
Size: 1356 1707
AleI CACnn'nnGTG
Cuts at: 0 784 3063
Size: 784 2279
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2281 2313 3063
Size: 2281 32 750
Fragments arranged by size:
2281 750 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2109 2355 3063
Size: 2109 246 708
Fragments arranged by size:
2109 708 246
ApaI G_GGCC'C
Cuts at: 0 240 300 1686 2461 3063
Size: 240 60 1386 775 602
Fragments arranged by size:
1386 775 602 240 60
ApaLI G'TGCA_C
Cuts at: 0 1480 3063
Size: 1480 1583
AvaI C'yCGr_G
Cuts at: 0 1175 1918 1985 3063
Size: 1175 743 67 1078
Fragments arranged by size:
1175 1078 743 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2536 2569 3063
Size: 2536 33 494
Fragments arranged by size:
2536 494 33
BanI G'GyrC_C
Cuts at: 0 247 364 440 778 1166 2122 3063
Size: 247 117 76 338 388 956 941
Fragments arranged by size:
956 941 388 338 247 117 76
BanII G_rGCy'C
Cuts at: 0 240 300 413 962 1406 1654 1686 2461
Size: 240 60 113 549 444 248 32 775
Cuts at: 2461 3052 3063
Size: 591 11
Fragments arranged by size:
775 591 549 444 248 240 113 60
32 11
BbeI G_GCGC'C
Cuts at: 0 1170 2126 3063
Size: 1170 956 937
Fragments arranged by size:
1170 956 937
BbsI GAAGACnn'nnnn_
Cuts at: 0 71 962 1595 1748 1845 2963 3063
Size: 71 891 633 153 97 1118 100
Fragments arranged by size:
1118 891 633 153 100 97 71
BbvCI CC'TCA_GC
Cuts at: 0 404 3063
Size: 404 2659
BciVI GTATCCnnnnn_n'
Cuts at: 0 904 2537 3063
Size: 904 1633 526
Fragments arranged by size:
1633 904 526
BglI GCCn_nnn'nGGC
Cuts at: 0 304 658 745 1055 1313 2104 2113 3063
Size: 304 354 87 310 258 791 9 950
Fragments arranged by size:
950 791 354 310 304 258 87 9
BlpI GC'TnA_GC
Cuts at: 0 1146 1405 1648 2942 2947 3063
Size: 1146 259 243 1294 5 116
Fragments arranged by size:
1294 1146 259 243 116 5
Bme1580I G_kGCm'C
Cuts at: 0 240 300 781 1267 1356 1463 1484 1686
Size: 240 60 481 486 89 107 21 202
Cuts at: 1686 2461 2709 3063
Size: 775 248 354
Fragments arranged by size:
775 486 481 354 248 240 202 107
89 60 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 15 2595 3063
Size: 15 2580 468
Fragments arranged by size:
2580 468 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 327 1072 2221 2553 2633 2905 3063
Size: 227 100 745 1149 332 80 272 158
Fragments arranged by size:
1149 745 332 272 227 158 100 80
Bpu10I CC'TnA_GC
Cuts at: 0 404 3063
Size: 404 2659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1727 3063
Size: 1727 1336
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 1615 3063
Size: 370 1245 1448
Fragments arranged by size:
1448 1245 370
BsaAI yAC'GTr
Cuts at: 0 630 1359 3063
Size: 630 729 1704
Fragments arranged by size:
1704 729 630
BsaHI Gr'CG_yC
Cuts at: 0 198 1167 2123 3063
Size: 198 969 956 940
Fragments arranged by size:
969 956 940 198
BsaWI w'CCGG_w
Cuts at: 0 118 1767 3063
Size: 118 1649 1296
Fragments arranged by size:
1649 1296 118
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 856 886 1089 1114 1119 1144 2281 2311
Size: 856 30 203 25 5 25 1137 30
Cuts at: 2311 2890 2920 3063
Size: 579 30 143
Fragments arranged by size:
1137 856 579 203 143 30 30 30
25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 486 489 492 495 498 501 504
Size: 421 65 3 3 3 3 3 3
Cuts at: 504 820 941 1409 2032 3063
Size: 316 121 468 623 1031
Fragments arranged by size:
1031 623 468 421 316 121 65 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 556 748 976 1026 1182 1760 2166 2477
Size: 556 192 228 50 156 578 406 311
Cuts at: 2477 3063
Size: 586
Fragments arranged by size:
586 578 556 406 311 228 192 156
50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1080 1399 2992 3063
Size: 835 245 319 1593 71
Fragments arranged by size:
1593 835 319 245 71
BsiEI CG_ry'CG
Cuts at: 0 1850 3063
Size: 1850 1213
BsiHKAI G_wGCw'C
Cuts at: 0 707 1406 1484 3063
Size: 707 699 78 1579
Fragments arranged by size:
1579 707 699 78
BsmI GAATG_Cn'
Cuts at: 0 987 3063
Size: 987 2076
BsmBI CGTCTCn'nnnn_
Cuts at: 0 724 3063
Size: 724 2339
Bsp1286I G_dGCh'C
Cuts at: 0 240 300 413 707 781 962 1267 1356
Size: 240 60 113 294 74 181 305 89
Cuts at: 1356 1406 1463 1484 1654 1686 2461 2709 3052
Size: 50 57 21 170 32 775 248 343
Cuts at: 3052 3063
Size: 11
Fragments arranged by size:
775 343 305 294 248 240 181 170
113 89 74 60 57 50 32 21
11
BspEI T'CCGG_A
Cuts at: 0 1767 3063
Size: 1767 1296
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 376 700 1208 1254 2969 3063
Size: 376 324 508 46 1715 94
Fragments arranged by size:
1715 508 376 324 94 46
BsrBI CCG'CTC
Cuts at: 0 288 359 594 3063
Size: 288 71 235 2469
Fragments arranged by size:
2469 288 235 71
BsrDI GCAATG_nn'
Cuts at: 0 2495 3063
Size: 2495 568
BsrFI r'CCGG_y
Cuts at: 0 1696 3063
Size: 1696 1367
BssSI C'ACGA_G
Cuts at: 0 923 3063
Size: 923 2140
BstEII G'GTnAC_C
Cuts at: 0 2211 3063
Size: 2211 852
BstXI CCAn_nnnn'nTGG
Cuts at: 0 164 556 1543 1666 2883 3063
Size: 164 392 987 123 1217 180
Fragments arranged by size:
1217 987 392 180 164 123
Bsu36I CC'TnA_GG
Cuts at: 0 1421 2278 2453 3037 3063
Size: 1421 857 175 584 26
Fragments arranged by size:
1421 857 584 175 26
BtgI C'CryG_G
Cuts at: 0 549 952 1701 2071 3063
Size: 549 403 749 370 992
Fragments arranged by size:
992 749 549 403 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2362 3063
Size: 2362 701
BtsI GCAGTG_nn'
Cuts at: 0 852 1628 2168 3063
Size: 852 776 540 895
Fragments arranged by size:
895 852 776 540
DraIII CAC_nnn'GTG
Cuts at: 0 282 1488 2400 2421 3063
Size: 282 1206 912 21 642
Fragments arranged by size:
1206 912 642 282 21
EaeI y'GGCC_r
Cuts at: 0 1522 1656 1698 2096 2318 2442 2988 3063
Size: 1522 134 42 398 222 124 546 75
Fragments arranged by size:
1522 546 398 222 134 124 75 42
EarI CTCTTCn'nnn_
Cuts at: 0 92 522 685 1210 3063
Size: 92 430 163 525 1853
Fragments arranged by size:
1853 525 430 163 92
EcoICRI GAG'CTC
Cuts at: 0 1404 3063
Size: 1404 1659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 65 227 327 1072 2221 2553 2633
Size: 29 36 162 100 745 1149 332 80
Cuts at: 2633 2905 2913 3010 3063
Size: 272 8 97 53
Fragments arranged by size:
1149 745 332 272 162 100 97 80
53 36 29 8
EcoO109I rG'GnC_Cy
Cuts at: 0 236 237 296 544 650 755 1089 1197
Size: 236 1 59 248 106 105 334 108
Cuts at: 1197 1956 2106 2457 2787 2811 3063
Size: 759 150 351 330 24 252
Fragments arranged by size:
759 351 334 330 252 248 236 150
108 106 105 59 24 1
HaeII r_GCGC'y
Cuts at: 0 1170 2126 3063
Size: 1170 956 937
Fragments arranged by size:
1170 956 937
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1725 1757 2890 2922 3063
Size: 1725 32 1133 32 141
Fragments arranged by size:
1725 1133 141 32 32
KasI G'GCGC_C
Cuts at: 0 1166 2122 3063
Size: 1166 956 941
Fragments arranged by size:
1166 956 941
MfeI C'AATT_G
Cuts at: 0 261 470 3063
Size: 261 209 2593
Fragments arranged by size:
2593 261 209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1526 2663 3063
Size: 1100 426 1137 400
Fragments arranged by size:
1137 1100 426 400
MscI TGG'CCA
Cuts at: 0 1658 2098 2320 2444 2990 3063
Size: 1658 440 222 124 546 73
Fragments arranged by size:
1658 546 440 222 124 73
MslI CAynn'nnrTG
Cuts at: 0 784 1513 1664 1837 3063
Size: 784 729 151 173 1226
Fragments arranged by size:
1226 784 729 173 151
MspA1I CmG'CkG
Cuts at: 0 564 772 899 1253 1277 1647 1860 1893
Size: 564 208 127 354 24 370 213 33
Cuts at: 1893 2286 2313 2328 2730 3063
Size: 393 27 15 402 333
Fragments arranged by size:
564 402 393 370 354 333 213 208
127 33 27 24 15
NaeI GCC'GGC
Cuts at: 0 1698 3063
Size: 1698 1365
NarI GG'CG_CC
Cuts at: 0 1167 2123 3063
Size: 1167 956 940
Fragments arranged by size:
1167 956 940
NgoMIV G'CCGG_C
Cuts at: 0 1696 3063
Size: 1696 1367
NspI r_CATG'y
Cuts at: 0 224 341 3063
Size: 224 117 2722
Fragments arranged by size:
2722 224 117
PasI CC'CwG_GG
Cuts at: 0 2791 2970 3063
Size: 2791 179 93
Fragments arranged by size:
2791 179 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 2733 3063
Size: 2733 330
PfoI T'CCnGG_A
Cuts at: 0 638 1553 2661 3063
Size: 638 915 1108 402
Fragments arranged by size:
1108 915 638 402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2281 2313 2614 2646 3063
Size: 2281 32 301 32 417
Fragments arranged by size:
2281 417 301 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 544 1197 1956 2787 3063
Size: 544 653 759 831 276
Fragments arranged by size:
831 759 653 544 276
PshAI GACnn'nnGTC
Cuts at: 0 1961 3063
Size: 1961 1102
PspOMI G'GGCC_C
Cuts at: 0 236 296 1682 2457 3063
Size: 236 60 1386 775 606
Fragments arranged by size:
1386 775 606 236 60
PstI C_TGCA'G
Cuts at: 0 531 1282 1431 1898 2069 2138 2312 2777
Size: 531 751 149 467 171 69 174 465
Cuts at: 2777 3063
Size: 286
Fragments arranged by size:
751 531 467 465 286 174 171 149
69
PvuII CAG'CTG
Cuts at: 0 772 1647 1893 2313 2730 3063
Size: 772 875 246 420 417 333
Fragments arranged by size:
875 772 420 417 333 246
SacI G_AGCT'C
Cuts at: 0 1406 3063
Size: 1406 1657
SanDI GG'GwC_CC
Cuts at: 0 544 2787 3063
Size: 544 2243 276
Fragments arranged by size:
2243 544 276
SapI GCTCTTCn'nnn_
Cuts at: 0 685 1210 3063
Size: 685 525 1853
Fragments arranged by size:
1853 685 525
SbfI CC_TGCA'GG
Cuts at: 0 2777 3063
Size: 2777 286
SexAI A'CCwGG_T
Cuts at: 0 1484 3063
Size: 1484 1579
SfcI C'TryA_G
Cuts at: 0 527 1278 1427 1894 2065 2134 2308 2773
Size: 527 751 149 467 171 69 174 465
Cuts at: 2773 3063
Size: 290
Fragments arranged by size:
751 527 467 465 290 174 171 149
69
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 658 2104 2113 3063
Size: 658 1446 9 950
Fragments arranged by size:
1446 950 658 9
SfoI GGC'GCC
Cuts at: 0 1168 2124 3063
Size: 1168 956 939
Fragments arranged by size:
1168 956 939
SmaI CCC'GGG
Cuts at: 0 1987 3063
Size: 1987 1076
SmlI C'TyrA_G
Cuts at: 0 1742 3063
Size: 1742 1321
SphI G_CATG'C
Cuts at: 0 341 3063
Size: 341 2722
StuI AGG'CCT
Cuts at: 0 937 1316 2802 2863 3063
Size: 937 379 1486 61 200
Fragments arranged by size:
1486 937 379 200 61
StyI C'CwwG_G
Cuts at: 0 458 1781 3063
Size: 458 1323 1282
Fragments arranged by size:
1323 1282 458
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 51 340 628 3063
Size: 51 289 288 2435
Fragments arranged by size:
2435 289 288 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 856 888 1089 1121 1854 1886 1907 1939
Size: 856 32 201 32 733 32 21 32
Cuts at: 1939 3063
Size: 1124
Fragments arranged by size:
1124 856 733 201 32 32 32 32
21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 161 1839 3063
Size: 161 1678 1224
Fragments arranged by size:
1678 1224 161
XmaI C'CCGG_G
Cuts at: 0 1985 3063
Size: 1985 1078
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApaLI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BciVI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssSI BstEII BstXI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PshAI
PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SmaI SmlI SphI StuI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApoI AscI AseI AsiSI AvrII BamHI BcgI
BclI BfrBI BglII BmgBI BmtI BplI BsaBI BsiWI
BspHI BsrGI BssHII BstAPI BstBI BstYI BstZ17I ClaI
CspCI DraI DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacII SalI ScaI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI