(Circular) (Six-base) MAPSORT of: pFN21AB0056.seq Check: 9301 from: 1 to: 9304 pFN21AB0056 9304 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3547 3577 4333 5275 6220 6250 3547 Size: 30 756 942 945 30 6601 Fragments arranged by size: 6601 945 942 756 30 30 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7114 278 Size: 53 83 186 936 5578 2468 Fragments arranged by size: 5578 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6084 6450 6647 1937 Size: 4147 366 197 4594 Fragments arranged by size: 4594 4147 366 197 Acc65I G'GTAC_C Cuts at: 6625 6625 Size: 9304 AclI AA'CG_TT Cuts at: 1563 7431 7804 1563 Size: 5868 373 3063 Fragments arranged by size: 5868 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2700 3138 3351 3663 3992 5948 6030 7367 Size: 1068 438 213 312 329 1956 82 1337 Cuts at: 7367 8751 9019 1632 Size: 1384 268 1917 Fragments arranged by size: 1956 1917 1384 1337 1068 438 329 312 268 213 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9107 AflIII A'CryG_T Cuts at: 1203 8203 9180 1203 Size: 7000 977 1327 Fragments arranged by size: 7000 1327 977 AgeI A'CCGG_T Cuts at: 4354 8135 4354 Size: 3781 5523 AhdI GACnn_n'nnGTC Cuts at: 8033 8033 Size: 9304 AleI CACnn'nnGTG Cuts at: 1166 3710 1166 Size: 2544 6760 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9304 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6098 6130 6098 Size: 32 9272 AlwNI CAG_nnn'CTG Cuts at: 2320 2335 3098 3329 3527 3970 4570 5314 5372 Size: 15 763 231 198 443 600 744 58 Cuts at: 5372 5501 5626 5836 5980 7047 8619 2320 Size: 129 125 210 144 1067 1572 3005 Fragments arranged by size: 3005 1572 1067 763 744 600 443 231 210 198 144 129 125 58 15 ApaI G_GGCC'C Cuts at: 2589 2666 2841 2867 2992 3260 3278 3406 4661 Size: 77 175 26 125 268 18 128 1255 Cuts at: 4661 4907 6173 6540 2589 Size: 246 1266 367 5353 Fragments arranged by size: 5353 1266 1255 367 268 246 175 128 125 77 26 18 ApaLI G'TGCA_C Cuts at: 7361 8517 7361 Size: 1156 8148 ApoI r'AATT_y Cuts at: 1493 2510 2611 6613 6839 6991 9100 1493 Size: 1017 101 4002 226 152 2109 1697 Fragments arranged by size: 4002 2109 1697 1017 226 152 101 AseI AT'TA_AT Cuts at: 160 1032 7858 160 Size: 872 6826 1606 Fragments arranged by size: 6826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9304 AvaI C'yCGr_G Cuts at: 1870 1943 2017 2144 2390 2502 2697 2868 4269 Size: 73 74 127 246 112 195 171 1401 Cuts at: 4269 4773 5301 6225 6528 6567 6629 1870 Size: 504 528 924 303 39 62 4545 Fragments arranged by size: 4545 1401 924 528 504 303 246 195 171 127 112 74 73 62 39 AvrII C'CTAG_G Cuts at: 4667 5252 4667 Size: 585 8719 BamHI G'GATC_C Cuts at: 4360 6634 7001 4360 Size: 2274 367 6663 Fragments arranged by size: 6663 2274 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3499 6625 8080 618 Size: 325 217 627 1712 3126 1455 1842 Fragments arranged by size: 3126 1842 1712 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2589 2666 2841 2867 2992 3260 3278 3406 Size: 1198 77 175 26 125 268 18 128 Cuts at: 3406 4016 4295 4661 4692 4892 4907 5034 5607 Size: 610 279 366 31 200 15 127 573 Cuts at: 5607 6055 6173 6540 6623 1391 Size: 448 118 367 83 4072 Fragments arranged by size: 4072 1198 610 573 448 367 366 279 268 200 175 128 127 125 118 83 77 31 26 18 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4381 4510 6424 928 Size: 971 2482 129 1914 3808 Fragments arranged by size: 3808 2482 1914 971 129 BbvCI CC'TCA_GC Cuts at: 3069 5797 3069 Size: 2728 6576 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7495 7529 1903 Size: 34 5558 34 3678 Fragments arranged by size: 5558 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2778 5640 6486 7196 8406 2778 Size: 2862 846 710 1210 3676 Fragments arranged by size: 3676 2862 1210 846 710 BclI T'GATC_A Cuts at: 1548 2950 1548 Size: 1402 7902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2871 5331 6033 Size: 107 122 71 631 605 1199 2460 702 Cuts at: 6033 7915 136 Size: 1882 1525 Fragments arranged by size: 2460 1882 1525 1199 702 631 605 122 107 71 BglII A'GATC_T Cuts at: 5673 9299 5673 Size: 3626 5678 BlpI GC'TnA_GC Cuts at: 2313 5559 6721 2313 Size: 3246 1162 4896 Fragments arranged by size: 4896 3246 1162 Bme1580I G_kGCm'C Cuts at: 1790 2434 2589 2666 2841 2867 2992 3260 3278 Size: 644 155 77 175 26 125 268 18 Cuts at: 3278 3406 3502 4038 4349 4661 4907 5592 6173 Size: 128 96 536 311 312 246 685 581 Cuts at: 6173 6540 7365 8521 1790 Size: 367 825 1156 2573 Fragments arranged by size: 2573 1156 825 685 644 581 536 367 312 311 268 246 175 155 128 125 96 77 26 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2722 3280 3761 Size: 416 53 83 399 24 1305 558 481 Cuts at: 3761 3833 4025 5402 5535 5536 5931 7035 7993 Size: 72 192 1377 133 1 395 1104 958 Cuts at: 7993 442 Size: 1753 Fragments arranged by size: 1753 1377 1305 1104 958 558 481 416 399 395 192 133 83 72 53 24 1 BmtI G_CTAG'C Cuts at: 1056 6264 1056 Size: 5208 4096 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4494 5626 5909 4494 Size: 1132 283 7889 Fragments arranged by size: 7889 1132 283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2163 2678 3183 3582 3594 3669 4193 4467 Size: 924 515 505 399 12 75 524 274 Cuts at: 4467 5214 5904 7964 1239 Size: 747 690 2060 2579 Fragments arranged by size: 2579 2060 924 747 690 524 515 505 399 274 75 12 Bpu10I CC'TnA_GC Cuts at: 2875 3069 5797 6498 2875 Size: 194 2728 701 5681 Fragments arranged by size: 5681 2728 701 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3005 3458 4279 5144 5807 6386 7429 8294 8592 Size: 453 821 865 663 579 1043 865 298 Cuts at: 8592 3005 Size: 3717 Fragments arranged by size: 3717 1043 865 865 821 663 579 453 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5251 7967 882 Size: 617 3752 2716 2219 Fragments arranged by size: 3752 2716 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2079 7095 493 Size: 711 875 5016 2702 Fragments arranged by size: 5016 2702 875 711 BsaBI GATnn'nnATC Cuts at: 9298 9298 Size: 9304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2351 7111 7493 8128 Size: 53 83 186 936 818 4760 382 635 Cuts at: 8128 275 Size: 1451 Fragments arranged by size: 4760 1451 936 818 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4354 7737 8135 8409 8556 8967 4354 Size: 3383 398 274 147 411 4691 Fragments arranged by size: 4691 3383 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2583 2613 3544 3574 3667 Size: 30 165 30 1180 30 931 30 93 Cuts at: 3667 3697 6098 6128 6548 6572 6578 6602 1178 Size: 30 2401 30 420 24 6 24 3880 Fragments arranged by size: 3880 2401 1180 931 420 165 93 30 30 30 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3074 3440 3813 3927 4196 4470 4483 4748 Size: 1888 366 373 114 269 274 13 265 Cuts at: 4748 4761 4857 5191 5602 5901 6000 6569 1186 Size: 13 96 334 411 299 99 569 3921 Fragments arranged by size: 3921 1888 569 411 373 366 334 299 274 269 265 114 99 96 13 13 BseYI C'CCAG_C Cuts at: 2380 2840 3322 3556 4502 4575 5006 5606 5654 Size: 460 482 234 946 73 431 600 48 Cuts at: 5654 5785 5866 6060 6188 6441 8507 9003 2380 Size: 131 81 194 128 253 2066 496 2681 Fragments arranged by size: 2681 2066 946 600 496 482 460 431 253 234 194 131 128 81 73 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3576 4590 5729 1163 Size: 573 1840 1014 1139 4738 Fragments arranged by size: 4738 1840 1139 1014 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2014 6754 7515 8543 1540 Size: 275 186 13 4740 761 1028 2301 Fragments arranged by size: 4740 2301 1028 761 275 186 13 BsiHKAI G_wGCw'C Cuts at: 2458 4126 7365 7450 8521 2458 Size: 1668 3239 85 1071 3241 Fragments arranged by size: 3241 3239 1668 1071 85 BsmI GAATG_Cn' Cuts at: 5218 6815 6908 5218 Size: 1597 93 7614 Fragments arranged by size: 7614 1597 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6404 6404 Size: 9304 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2434 2458 2589 2666 2841 2867 2992 Size: 399 644 24 131 77 175 26 125 Cuts at: 2992 3260 3278 3406 3502 4016 4038 4126 4295 Size: 268 18 128 96 514 22 88 169 Cuts at: 4295 4349 4661 4692 4892 4907 5034 5592 5607 Size: 54 312 31 200 15 127 558 15 Cuts at: 5607 6055 6173 6540 6623 7365 7450 8521 1391 Size: 448 118 367 83 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 558 514 448 399 367 312 268 200 175 169 131 128 127 125 118 96 88 85 83 77 54 31 26 24 22 18 15 15 BspEI T'CCGG_A Cuts at: 8967 8967 Size: 9304 BspHI T'CATG_A Cuts at: 2689 7193 8923 2689 Size: 4504 1730 3070 Fragments arranged by size: 4504 3070 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3547 3577 4333 5275 6220 6250 6659 844 Size: 2703 30 756 942 945 30 409 3489 Fragments arranged by size: 3489 2703 945 942 756 409 30 30 BsrBI CCG'CTC Cuts at: 1394 1823 2312 3400 3430 4819 6511 7191 1394 Size: 429 489 1088 30 1389 1692 680 3507 Fragments arranged by size: 3507 1692 1389 1088 680 489 429 30 BsrDI GCAATG_nn' Cuts at: 65 1248 2157 2224 7799 7973 65 Size: 1183 909 67 5575 174 1396 Fragments arranged by size: 5575 1396 1183 909 174 67 BsrFI r'CCGG_y Cuts at: 1700 1810 2338 4131 4354 5001 5878 7948 8135 Size: 110 528 1793 223 647 877 2070 187 Cuts at: 8135 1700 Size: 2869 Fragments arranged by size: 2869 2070 1793 877 647 528 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9304 BssSI C'ACGA_G Cuts at: 5520 7364 8376 5520 Size: 1844 1012 6448 Fragments arranged by size: 6448 1844 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2335 3461 3527 5314 794 Size: 1541 1126 66 1787 4784 Fragments arranged by size: 4784 1787 1541 1126 66 BstBI TT'CG_AA Cuts at: 3756 8108 3756 Size: 4352 4952 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9304 BstXI CCAn_nnnn'nTGG Cuts at: 3769 5014 5399 5578 3769 Size: 1245 385 179 7495 Fragments arranged by size: 7495 1245 385 179 BstYI r'GATC_y Cuts at: 1972 2100 4360 5673 5977 6634 7001 7386 7403 Size: 128 2260 1313 304 657 367 385 17 Cuts at: 7403 8855 8941 8953 9299 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2260 1977 1452 1313 657 385 367 346 304 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4653 4899 4914 5614 5634 4653 Size: 246 15 700 20 8323 Fragments arranged by size: 8323 700 246 20 15 BtgI C'CryG_G Cuts at: 513 1065 2128 2449 2743 5013 5688 6078 513 Size: 552 1063 321 294 2270 675 390 3739 Fragments arranged by size: 3739 2270 1063 675 552 390 321 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3045 487 Size: 683 56 1819 6746 Fragments arranged by size: 6746 1819 683 56 BtsI GCAGTG_nn' Cuts at: 2333 3399 3751 5656 5741 5801 6820 7614 7634 Size: 1066 352 1905 85 60 1019 794 20 Cuts at: 7634 2333 Size: 4003 Fragments arranged by size: 4003 1905 1066 1019 794 352 85 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9269 DraI TTT'AAA Cuts at: 6608 6608 Size: 9304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2228 8311 809 Size: 507 912 6083 1802 Fragments arranged by size: 6083 1802 912 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5384 6025 6751 Size: 54 1425 50 275 18 3554 641 726 Cuts at: 6751 7640 9269 8 Size: 889 1629 43 Fragments arranged by size: 3554 1629 1425 889 726 641 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6751 1537 Size: 275 4939 4090 Fragments arranged by size: 4939 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2991 3417 3438 3639 3807 7234 1353 Size: 1638 426 21 201 168 3427 3423 Fragments arranged by size: 3427 3423 1638 426 201 168 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2591 2781 4511 5714 7891 8265 Size: 1064 138 1002 190 1730 1203 2177 374 Cuts at: 8265 8411 387 Size: 146 1280 Fragments arranged by size: 2177 1730 1280 1203 1064 1002 374 190 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2678 2700 3138 3183 3351 3582 Size: 393 531 515 22 438 45 168 231 Cuts at: 3582 3594 3663 3669 3992 4193 4467 5214 5904 Size: 12 69 6 323 201 274 747 690 Cuts at: 5904 5948 6030 7367 7964 8751 9019 1239 Size: 44 82 1337 597 787 268 1524 Fragments arranged by size: 1524 1337 787 747 690 597 531 515 438 393 323 274 268 231 201 168 82 69 45 44 22 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 3684 4666 5151 6531 3684 Size: 982 485 1380 6457 Fragments arranged by size: 6457 1380 982 485 EcoO109I rG'GnC_Cy Cuts at: 2662 2810 2864 2988 3215 3256 3257 3274 3275 Size: 148 54 124 227 41 1 17 1 Cuts at: 3275 3305 3658 3797 4108 4657 4658 4671 4903 Size: 30 353 139 311 549 1 13 232 Cuts at: 4903 4910 4945 5123 5177 6170 6249 6341 6375 Size: 7 35 178 54 993 79 92 34 Cuts at: 6375 6536 6537 6564 2662 Size: 161 1 27 5402 Fragments arranged by size: 5402 993 549 353 311 232 227 178 161 148 139 124 92 79 54 54 41 35 34 30 27 17 13 7 1 1 1 1 EcoRI G'AATT_C Cuts at: 6613 6991 6613 Size: 378 8926 FseI GG_CCGG'CC Cuts at: 4135 4135 Size: 9304 FspI TGC'GCA Cuts at: 7810 7810 Size: 9304 HaeII r_GCGC'y Cuts at: 1678 3451 5626 8451 1678 Size: 1773 2175 2825 2531 Fragments arranged by size: 2825 2531 2175 1773 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2583 2615 3074 3106 4168 Size: 32 135 32 1011 32 459 32 1062 Cuts at: 4168 4200 5420 5452 5552 5584 5969 6001 6548 Size: 32 1220 32 100 32 385 32 547 Cuts at: 6548 6580 7945 7977 8019 8051 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 1220 1062 1011 547 459 385 135 100 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3004 4702 6451 6648 6894 669 Size: 988 281 1066 1698 1749 197 246 3079 Fragments arranged by size: 3079 1749 1698 1066 988 281 246 197 HindIII A'AGCT_T Cuts at: 748 748 Size: 9304 HpaI GTT'AAC Cuts at: 6894 6894 Size: 9304 KpnI G_GTAC'C Cuts at: 6629 6629 Size: 9304 MfeI C'AATT_G Cuts at: 6903 6903 Size: 9304 MluI A'CGCG_T Cuts at: 9180 9180 Size: 9304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2307 2385 2540 2937 4585 8418 8602 1305 Size: 1002 78 155 397 1648 3833 184 2007 Fragments arranged by size: 3833 2007 1648 1002 397 184 155 78 MscI TGG'CCA Cuts at: 10 64 1489 1832 6027 10 Size: 54 1425 343 4195 3287 Fragments arranged by size: 4195 3287 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2463 2688 3710 5066 7262 7621 7780 Size: 648 1297 225 1022 1356 2196 359 159 Cuts at: 7780 518 Size: 2042 Fragments arranged by size: 2196 2042 1356 1297 1022 648 359 225 159 MspA1I CmG'CkG Cuts at: 1606 2114 2320 2745 3949 4406 4707 6094 6720 Size: 508 206 425 1204 457 301 1387 626 Cuts at: 6720 7397 8545 8790 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1387 1204 1148 677 626 508 457 425 301 245 206 NaeI GCC'GGC Cuts at: 2340 4133 5880 2340 Size: 1793 1747 5764 Fragments arranged by size: 5764 1793 1747 NcoI C'CATG_G Cuts at: 513 1065 2128 2449 5013 5688 6078 513 Size: 552 1063 321 2564 675 390 3739 Fragments arranged by size: 3739 2564 1063 675 552 390 321 NdeI CA'TA_TG Cuts at: 387 387 Size: 9304 NgoMIV G'CCGG_C Cuts at: 2338 4131 5878 2338 Size: 1793 1747 5764 Fragments arranged by size: 5764 1793 1747 NheI G'CTAG_C Cuts at: 1052 6260 1052 Size: 5208 4096 NotI GC'GGCC_GC Cuts at: 6751 6751 Size: 9304 NspI r_CATG'y Cuts at: 1132 1229 3439 6662 8207 1132 Size: 97 2210 3223 1545 2229 Fragments arranged by size: 3223 2229 2210 1545 97 PasI CC'CwG_GG Cuts at: 1348 2806 3281 4063 5729 1348 Size: 1458 475 782 1666 4923 Fragments arranged by size: 4923 1666 1458 782 475 PciI A'CATG_T Cuts at: 8203 8203 Size: 9304 PflMI CCAn_nnn'nTGG Cuts at: 1108 3299 5714 5836 1108 Size: 2191 2415 122 4576 Fragments arranged by size: 4576 2415 2191 122 PfoI T'CCnGG_A Cuts at: 5598 6306 5598 Size: 708 8596 PmeI GTTT'AAAC Cuts at: 6608 6608 Size: 9304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6098 6130 7373 7405 8886 8918 6098 Size: 32 1243 32 1481 32 6484 Fragments arranged by size: 6484 1481 1243 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3215 3305 3797 5123 5177 6341 6375 6564 3215 Size: 90 492 1326 54 1164 34 189 5955 Fragments arranged by size: 5955 1326 1164 492 189 90 54 34 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9304 PsiI TTA'TAA Cuts at: 6874 6874 Size: 9304 PspOMI G'GGCC_C Cuts at: 2585 2662 2837 2863 2988 3256 3274 3402 4657 Size: 77 175 26 125 268 18 128 1255 Cuts at: 4657 4903 6169 6536 2585 Size: 246 1266 367 5353 Fragments arranged by size: 5353 1266 1255 367 268 246 175 128 125 77 26 18 PstI C_TGCA'G Cuts at: 830 2139 2526 3574 5132 6018 6183 6656 830 Size: 1309 387 1048 1558 886 165 473 3478 Fragments arranged by size: 3478 1558 1309 1048 886 473 387 165 PvuI CG_AT'CG Cuts at: 2001 2014 2001 Size: 13 9291 PvuII CAG'CTG Cuts at: 6094 6094 Size: 9304 SacII CC_GC'GG Cuts at: 2746 2746 Size: 9304 SalI G'TCGA_C Cuts at: 1936 6449 6646 1936 Size: 4513 197 4594 Fragments arranged by size: 4594 4513 197 SanDI GG'GwC_CC Cuts at: 5177 6564 5177 Size: 1387 7917 SapI GCTCTTCn'nnn_ Cuts at: 3438 3639 3438 Size: 201 9103 SbfI CC_TGCA'GG Cuts at: 2526 6656 2526 Size: 4130 5174 ScaI AGT'ACT Cuts at: 7552 9264 7552 Size: 1712 7592 SexAI A'CCwGG_T Cuts at: 5398 5398 Size: 9304 SfcI C'TryA_G Cuts at: 826 1045 2135 2522 2708 3570 4844 5128 5859 Size: 219 1090 387 186 862 1274 284 731 Cuts at: 5859 6014 6179 6421 6652 7787 8468 8659 826 Size: 155 165 242 231 1135 681 191 1471 Fragments arranged by size: 1471 1274 1135 1090 862 731 681 387 284 242 231 219 191 186 165 155 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9304 SgrAI Cr'CCGG_yG Cuts at: 1700 4354 1700 Size: 2654 6650 SmaI CCC'GGG Cuts at: 1872 2699 5303 6631 1872 Size: 827 2604 1328 4545 Fragments arranged by size: 4545 2604 1328 827 SmlI C'TyrA_G Cuts at: 820 1017 1943 2502 3020 3473 4294 5159 5822 Size: 197 926 559 518 453 821 865 663 Cuts at: 5822 6401 6528 7408 8309 8571 820 Size: 579 127 880 901 262 1553 Fragments arranged by size: 1553 926 901 880 865 821 663 579 559 518 453 262 197 127 SnaBI TAC'GTA Cuts at: 493 2079 7095 493 Size: 1586 5016 2702 Fragments arranged by size: 5016 2702 1586 SpeI A'CTAG_T Cuts at: 152 2236 5423 152 Size: 2084 3187 4033 Fragments arranged by size: 4033 3187 2084 SphI G_CATG'C Cuts at: 1132 3439 6662 1132 Size: 2307 3223 3774 Fragments arranged by size: 3774 3223 2307 SspI AAT'ATT Cuts at: 5 52 7228 5 Size: 47 7176 2081 Fragments arranged by size: 7176 2081 47 StuI AGG'CCT Cuts at: 1517 5191 6467 1517 Size: 3674 1276 4354 Fragments arranged by size: 4354 3674 1276 StyI C'CwwG_G Cuts at: 513 1065 2031 2128 2449 2553 3858 4104 4622 Size: 552 966 97 321 104 1305 246 518 Cuts at: 4622 4667 5013 5021 5252 5688 6078 6334 6503 Size: 45 346 8 231 436 390 256 169 Cuts at: 6503 6743 513 Size: 240 3074 Fragments arranged by size: 3074 1305 966 552 518 436 390 346 321 256 246 240 231 169 104 97 45 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2462 4156 4751 5373 6455 7331 7348 Size: 1255 882 1694 595 622 1082 876 17 Cuts at: 7348 7501 7686 8131 325 Size: 153 185 445 1498 Fragments arranged by size: 1694 1498 1255 1082 882 876 622 595 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7550 9262 96 Size: 274 80 33 51 1443 5573 1712 138 Fragments arranged by size: 5573 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2715 2747 3068 3100 3558 3590 5895 5927 9264 Size: 32 321 32 458 32 2305 32 3337 Cuts at: 9264 9296 2715 Size: 32 2723 Fragments arranged by size: 3337 2723 2305 458 321 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5400 1316 Size: 4084 5220 XbaI T'CTAG_A Cuts at: 4225 6640 4225 Size: 2415 6889 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2456 3095 4371 2456 Size: 639 1276 7389 Fragments arranged by size: 7389 1276 639 XhoI C'TCGA_G Cuts at: 1943 2502 6528 1943 Size: 559 4026 4719 Fragments arranged by size: 4719 4026 559 XmaI C'CCGG_G Cuts at: 1870 2697 5301 6629 1870 Size: 827 2604 1328 4545 Fragments arranged by size: 4545 2604 1328 827 XmnI GAAnn'nnTTC Cuts at: 3816 7433 3816 Size: 3617 5687 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7112 276 Size: 53 83 186 936 5578 2468 Fragments arranged by size: 5578 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SfiI SfoI SrfI SwaI