(Circular) (Six-base) MAPSORT of: pFN21AB0053.seq Check: 8099 from: 1 to: 8641 pFN21AB0053 8641 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6451 278 Size: 53 83 186 936 4915 2468 Fragments arranged by size: 4915 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3099 5984 1937 Size: 1162 2885 4594 Fragments arranged by size: 4594 2885 1162 Acc65I G'GTAC_C Cuts at: 5962 5962 Size: 8641 AclI AA'CG_TT Cuts at: 1563 6768 7141 1563 Size: 5205 373 3063 Fragments arranged by size: 5205 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2658 2927 4167 4572 4944 5274 5781 6704 Size: 1026 269 1240 405 372 330 507 923 Cuts at: 6704 8088 8356 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1240 1026 923 507 405 372 330 269 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8444 AflIII A'CryG_T Cuts at: 1203 7540 8517 1203 Size: 6337 977 1327 Fragments arranged by size: 6337 1327 977 AgeI A'CCGG_T Cuts at: 7472 7472 Size: 8641 AhdI GACnn_n'nnGTC Cuts at: 5248 7370 5248 Size: 2122 6519 AleI CACnn'nnGTG Cuts at: 1166 2048 1166 Size: 882 7759 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8641 AlwNI CAG_nnn'CTG Cuts at: 4844 5105 5870 6384 7956 4844 Size: 261 765 514 1572 5529 Fragments arranged by size: 5529 1572 765 514 261 ApaI G_GGCC'C Cuts at: 2180 2180 Size: 8641 ApaLI G'TGCA_C Cuts at: 6698 7854 6698 Size: 1156 7485 ApoI r'AATT_y Cuts at: 1493 2666 2765 2957 3013 3127 3212 3424 5033 Size: 1173 99 192 56 114 85 212 1609 Cuts at: 5033 5950 6176 6328 8437 1493 Size: 917 226 152 2109 1697 Fragments arranged by size: 2109 1697 1609 1173 917 226 212 192 152 114 99 85 56 AseI AT'TA_AT Cuts at: 160 1032 7195 160 Size: 872 6163 1606 Fragments arranged by size: 6163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8641 AvaI C'yCGr_G Cuts at: 1870 1943 5966 1870 Size: 73 4023 4545 Fragments arranged by size: 4545 4023 73 BamHI G'GATC_C Cuts at: 5605 5971 6338 5605 Size: 366 367 7908 Fragments arranged by size: 7908 367 366 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5962 7417 618 Size: 325 217 627 4175 1455 1842 Fragments arranged by size: 4175 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2180 5238 5297 5960 1391 Size: 789 3058 59 663 4072 Fragments arranged by size: 4072 3058 789 663 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2306 2457 2807 3495 4155 5124 928 Size: 971 407 151 350 688 660 969 4445 Fragments arranged by size: 4445 971 969 688 660 407 350 151 BbvCI CC'TCA_GC Cuts at: 3564 3564 Size: 8641 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6832 6866 1903 Size: 34 4895 34 3678 Fragments arranged by size: 4895 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3134 4051 6533 7743 3134 Size: 917 2482 1210 4032 Fragments arranged by size: 4032 2482 1210 917 BclI T'GATC_A Cuts at: 1548 3454 4219 1548 Size: 1906 765 5970 Fragments arranged by size: 5970 1906 765 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 7252 136 Size: 107 122 71 631 605 337 5243 1525 Fragments arranged by size: 5243 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2438 3106 8636 2438 Size: 668 5530 2443 Fragments arranged by size: 5530 2443 668 BlpI GC'TnA_GC Cuts at: 6058 6058 Size: 8641 Bme1580I G_kGCm'C Cuts at: 1790 2045 2180 6702 7858 1790 Size: 255 135 4522 1156 2573 Fragments arranged by size: 4522 2573 1156 255 135 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4938 5619 6372 Size: 416 53 83 399 24 3521 681 753 Cuts at: 6372 7330 442 Size: 958 1753 Fragments arranged by size: 3521 1753 958 753 681 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8641 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4194 4194 Size: 8641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 2461 3606 4323 4953 5231 5559 7301 Size: 1149 73 1145 717 630 278 328 1742 Cuts at: 7301 1239 Size: 2579 Fragments arranged by size: 2579 1742 1149 1145 717 630 328 278 73 Bpu10I CC'TnA_GC Cuts at: 2388 3564 5370 2388 Size: 1176 1806 5659 Fragments arranged by size: 5659 1806 1176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4164 5281 5600 6766 7631 7929 4164 Size: 1117 319 1166 865 298 4876 Fragments arranged by size: 4876 1166 1117 865 319 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7304 882 Size: 617 5805 2219 Fragments arranged by size: 5805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6432 493 Size: 711 5228 2702 Fragments arranged by size: 5228 2702 711 BsaBI GATnn'nnATC Cuts at: 5604 5610 8635 5604 Size: 6 3025 5610 Fragments arranged by size: 5610 3025 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6448 6830 7465 275 Size: 53 83 186 936 4915 382 635 1451 Fragments arranged by size: 4915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7074 7472 7746 7893 8304 7074 Size: 398 274 147 411 7411 Fragments arranged by size: 7411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2184 2214 3689 3719 5539 Size: 30 165 30 781 30 1475 30 1820 Cuts at: 5539 5557 5569 5587 5776 5806 1178 Size: 18 12 18 189 30 4013 Fragments arranged by size: 4013 1820 1475 781 189 165 30 30 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2082 2864 2867 3200 3344 3347 3650 3653 Size: 896 782 3 333 144 3 303 3 Cuts at: 3653 3656 3659 3710 4901 4916 4943 5391 5797 Size: 3 3 51 1191 15 27 448 406 Cuts at: 5797 5827 1186 Size: 30 4000 Fragments arranged by size: 4000 1191 896 782 448 406 333 303 144 51 30 27 15 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 5228 7844 8340 5228 Size: 2616 496 5529 Fragments arranged by size: 5529 2616 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2700 5956 1163 Size: 573 964 3256 3848 Fragments arranged by size: 3848 3256 964 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6091 6852 7880 1540 Size: 275 186 4090 761 1028 2301 Fragments arranged by size: 4090 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4180 5238 5297 6702 6787 7858 4180 Size: 1058 59 1405 85 1071 4963 Fragments arranged by size: 4963 1405 1071 1058 85 59 BsmI GAATG_Cn' Cuts at: 6152 6245 6152 Size: 93 8548 BsmBI CGTCTCn'nnnn_ Cuts at: 2302 2302 Size: 8641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2180 4180 5238 5297 5960 6702 Size: 399 255 135 2000 1058 59 663 742 Cuts at: 6702 6787 7858 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 2000 1071 1058 742 663 399 255 135 85 59 BspEI T'CCGG_A Cuts at: 8304 8304 Size: 8641 BspHI T'CATG_A Cuts at: 6530 8260 6530 Size: 1730 6911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3892 5264 5996 844 Size: 3048 1372 732 3489 Fragments arranged by size: 3489 3048 1372 732 BsrBI CCG'CTC Cuts at: 1394 1823 6528 1394 Size: 429 4705 3507 Fragments arranged by size: 4705 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3224 3257 7136 7310 65 Size: 1183 1976 33 3879 174 1396 Fragments arranged by size: 3879 1976 1396 1183 174 33 BsrFI r'CCGG_y Cuts at: 1700 1810 5121 7285 7472 1700 Size: 110 3311 2164 187 2869 Fragments arranged by size: 3311 2869 2164 187 110 BsrGI T'GTAC_A Cuts at: 96 2952 3043 5342 96 Size: 2856 91 2299 3395 Fragments arranged by size: 3395 2856 2299 91 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8641 BssSI C'ACGA_G Cuts at: 6701 7713 6701 Size: 1012 7629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8641 BstBI TT'CG_AA Cuts at: 5179 7445 5179 Size: 2266 6375 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8641 BstXI CCAn_nnnn'nTGG Cuts at: 3110 3110 Size: 8641 BstYI r'GATC_y Cuts at: 1972 2438 3106 4051 5605 5971 6338 6723 6740 Size: 466 668 945 1554 366 367 385 17 Cuts at: 6740 8192 8278 8290 8636 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1554 1452 945 668 466 385 367 366 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8089 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7902 Fragments arranged by size: 7902 683 56 BtsI GCAGTG_nn' Cuts at: 2712 2836 2896 4907 6157 6951 6971 2712 Size: 124 60 2011 1250 794 20 4382 Fragments arranged by size: 4382 2011 1250 794 124 60 20 ClaI AT'CG_AT Cuts at: 1554 5632 1554 Size: 4078 4563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5196 5231 529 Size: 35 4632 35 3939 Fragments arranged by size: 4632 3939 35 35 DraI TTT'AAA Cuts at: 2739 4731 5796 5945 2739 Size: 1992 1065 149 5435 Fragments arranged by size: 5435 1992 1065 149 DraIII CAC_nnn'GTG Cuts at: 4946 4946 Size: 8641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7648 809 Size: 507 6332 1802 Fragments arranged by size: 6332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6088 6977 8606 Size: 54 1425 50 275 18 4258 889 1629 Cuts at: 8606 8 Size: 43 Fragments arranged by size: 4258 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6088 1537 Size: 275 4276 4090 Fragments arranged by size: 4276 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 2841 3627 3654 3687 4491 4527 4591 Size: 1101 387 786 27 33 804 36 64 Cuts at: 4591 4920 5166 5845 6571 1353 Size: 329 246 679 726 3423 Fragments arranged by size: 3423 1101 804 786 726 679 387 329 246 64 36 33 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7228 7602 7748 387 Size: 1064 138 5639 374 146 1280 Fragments arranged by size: 5639 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5236 5295 5236 Size: 59 8582 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2461 2658 2927 3606 4167 4323 Size: 393 756 73 197 269 679 561 156 Cuts at: 4323 4572 4944 4953 5231 5274 5559 5781 6704 Size: 249 372 9 278 43 285 222 923 Cuts at: 6704 7301 8088 8356 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 923 787 756 679 597 561 393 372 285 278 269 268 249 222 197 156 73 43 9 EcoO109I rG'GnC_Cy Cuts at: 2011 2176 3808 2011 Size: 165 1632 6844 Fragments arranged by size: 6844 1632 165 EcoRI G'AATT_C Cuts at: 5950 6328 5950 Size: 378 8263 EcoRV GAT'ATC Cuts at: 3008 3008 Size: 8641 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2764 4574 5738 2764 Size: 1810 1164 5667 Fragments arranged by size: 5667 1810 1164 FspI TGC'GCA Cuts at: 7147 7147 Size: 8641 HaeII r_GCGC'y Cuts at: 1678 3997 7788 1678 Size: 2319 3791 2531 Fragments arranged by size: 3791 2531 2319 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2447 2453 2479 2485 3898 Size: 32 135 32 875 6 26 6 1413 Cuts at: 3898 3930 4772 4804 5234 5266 5557 5589 7282 Size: 32 842 32 430 32 291 32 1693 Cuts at: 7282 7314 7356 7388 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1693 1413 875 842 430 291 135 42 32 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3244 3472 5880 5985 6231 669 Size: 988 281 1306 228 2408 105 246 3079 Fragments arranged by size: 3079 2408 1306 988 281 246 228 105 HindIII A'AGCT_T Cuts at: 748 4128 748 Size: 3380 5261 HpaI GTT'AAC Cuts at: 3244 3472 5880 6231 3244 Size: 228 2408 351 5654 Fragments arranged by size: 5654 2408 351 228 KpnI G_GTAC'C Cuts at: 5966 5966 Size: 8641 MfeI C'AATT_G Cuts at: 2948 5208 6240 2948 Size: 2260 1032 5349 Fragments arranged by size: 5349 2260 1032 MluI A'CGCG_T Cuts at: 8517 8517 Size: 8641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5649 7755 7939 1305 Size: 4344 2106 184 2007 Fragments arranged by size: 4344 2106 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6819 Fragments arranged by size: 6819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2048 5531 6599 6958 7117 518 Size: 648 882 3483 1068 359 159 2042 Fragments arranged by size: 3483 2042 1068 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3134 5483 5867 6057 6734 7882 8127 1606 Size: 1528 2349 384 190 677 1148 245 2120 Fragments arranged by size: 2349 2120 1528 1148 677 384 245 190 NaeI GCC'GGC Cuts at: 5123 5123 Size: 8641 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8089 NdeI CA'TA_TG Cuts at: 387 387 Size: 8641 NgoMIV G'CCGG_C Cuts at: 5121 5121 Size: 8641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8641 NotI GC'GGCC_GC Cuts at: 6088 6088 Size: 8641 NspI r_CATG'y Cuts at: 1132 1229 5999 7544 1132 Size: 97 4770 1545 2229 Fragments arranged by size: 4770 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8641 PciI A'CATG_T Cuts at: 7540 7540 Size: 8641 PflMI CCAn_nnn'nTGG Cuts at: 1108 5045 1108 Size: 3937 4704 PfoI T'CCnGG_A Cuts at: 5575 5575 Size: 8641 PmeI GTTT'AAAC Cuts at: 5945 5945 Size: 8641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6710 6742 8223 8255 6710 Size: 32 1481 32 7096 Fragments arranged by size: 7096 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3808 3808 Size: 8641 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8641 PsiI TTA'TAA Cuts at: 3162 6211 3162 Size: 3049 5592 PspOMI G'GGCC_C Cuts at: 2176 2176 Size: 8641 PstI C_TGCA'G Cuts at: 830 3139 4861 4914 5993 830 Size: 2309 1722 53 1079 3478 Fragments arranged by size: 3478 2309 1722 1079 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8641 PvuII CAG'CTG Cuts at: 3134 5483 5867 3134 Size: 2349 384 5908 Fragments arranged by size: 5908 2349 384 SacI G_AGCT'C Cuts at: 5238 5297 5238 Size: 59 8582 SalI G'TCGA_C Cuts at: 1936 5983 1936 Size: 4047 4594 SapI GCTCTTCn'nnn_ Cuts at: 4591 4591 Size: 8641 SbfI CC_TGCA'GG Cuts at: 5993 5993 Size: 8641 ScaI AGT'ACT Cuts at: 3191 6889 8601 3191 Size: 3698 1712 3231 Fragments arranged by size: 3698 3231 1712 SfcI C'TryA_G Cuts at: 826 1045 2274 3135 3193 4857 4910 5989 7124 Size: 219 1229 861 58 1664 53 1079 1135 Cuts at: 7124 7805 7996 826 Size: 681 191 1471 Fragments arranged by size: 1664 1471 1229 1135 1079 861 681 219 191 58 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8641 SmaI CCC'GGG Cuts at: 1872 5968 1872 Size: 4096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4179 5296 5615 6745 7646 7908 Size: 197 926 2236 1117 319 1130 901 262 Cuts at: 7908 820 Size: 1553 Fragments arranged by size: 2236 1553 1130 1117 926 901 319 262 197 SnaBI TAC'GTA Cuts at: 493 6432 493 Size: 5939 2702 SpeI A'CTAG_T Cuts at: 152 2705 152 Size: 2553 6088 SphI G_CATG'C Cuts at: 1132 5999 1132 Size: 4867 3774 SspI AAT'ATT Cuts at: 5 52 2638 3070 3397 6565 5 Size: 47 2586 432 327 3168 2081 Fragments arranged by size: 3168 2586 2081 432 327 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8641 StyI C'CwwG_G Cuts at: 513 1065 6080 513 Size: 552 5015 3074 Fragments arranged by size: 5015 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4957 6668 6685 6838 7023 7468 325 Size: 1255 3377 1711 17 153 185 445 1498 Fragments arranged by size: 3377 1711 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2952 2998 3043 Size: 274 80 33 51 1443 975 46 45 Cuts at: 3043 3189 5342 6887 8599 96 Size: 146 2153 1545 1712 138 Fragments arranged by size: 2153 1712 1545 1443 975 274 146 138 80 51 46 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 3689 3721 3825 3857 8601 8633 2184 Size: 32 1473 32 104 32 4744 32 2192 Fragments arranged by size: 4744 2192 1473 104 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5569 1316 Size: 4253 4388 XbaI T'CTAG_A Cuts at: 5977 5977 Size: 8641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3737 3737 Size: 8641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8641 XmaI C'CCGG_G Cuts at: 1870 5966 1870 Size: 4096 4545 XmnI GAAnn'nnTTC Cuts at: 2916 4749 5175 5721 6770 2916 Size: 1833 426 546 1049 4787 Fragments arranged by size: 4787 1833 1049 546 426 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6449 276 Size: 53 83 186 936 4915 2468 Fragments arranged by size: 4915 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI