(Circular) (Six-base) MAPSORT of: pFN21AB0044.seq Check: 3716 from: 1 to: 8479 pFN21AB0044 8479 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6289 278 Size: 53 83 186 936 4753 2468 Fragments arranged by size: 4753 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5822 1937 Size: 3885 4594 Acc65I G'GTAC_C Cuts at: 3641 5800 3641 Size: 2159 6320 AclI AA'CG_TT Cuts at: 1563 6606 6979 1563 Size: 5043 373 3063 Fragments arranged by size: 5043 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1998 2481 2801 3068 4430 6542 7926 8194 Size: 366 483 320 267 1362 2112 1384 268 Cuts at: 8194 1632 Size: 1917 Fragments arranged by size: 2112 1917 1384 1362 483 366 320 268 267 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8282 AflIII A'CryG_T Cuts at: 1203 3883 7378 8355 1203 Size: 2680 3495 977 1327 Fragments arranged by size: 3495 2680 1327 977 AgeI A'CCGG_T Cuts at: 7310 7310 Size: 8479 AhdI GACnn_n'nnGTC Cuts at: 7208 7208 Size: 8479 AleI CACnn'nnGTG Cuts at: 1166 4219 1166 Size: 3053 5426 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2849 3671 3861 4677 1184 Size: 1665 822 190 816 4986 Fragments arranged by size: 4986 1665 822 816 190 AlwNI CAG_nnn'CTG Cuts at: 4342 4472 6222 7794 4342 Size: 130 1750 1572 5027 Fragments arranged by size: 5027 1750 1572 130 ApaLI G'TGCA_C Cuts at: 6536 7692 6536 Size: 1156 7323 ApoI r'AATT_y Cuts at: 1493 2465 2522 2543 4752 5030 5087 5152 5648 Size: 972 57 21 2209 278 57 65 496 Cuts at: 5648 5788 6014 6166 8275 1493 Size: 140 226 152 2109 1697 Fragments arranged by size: 2209 2109 1697 972 496 278 226 152 140 65 57 57 21 AseI AT'TA_AT Cuts at: 160 1032 4421 7033 160 Size: 872 3389 2612 1606 Fragments arranged by size: 3389 2612 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8479 AvaI C'yCGr_G Cuts at: 1870 1943 5804 1870 Size: 73 3861 4545 Fragments arranged by size: 4545 3861 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4973 5006 4973 Size: 33 8446 BamHI G'GATC_C Cuts at: 5809 6176 5809 Size: 367 8112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3641 5800 7255 618 Size: 325 217 627 1854 2159 1455 1842 Fragments arranged by size: 2159 1854 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3479 5592 5798 1391 Size: 2088 2113 206 4072 Fragments arranged by size: 4072 2113 2088 206 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3243 4032 4172 5499 928 Size: 971 1344 789 140 1327 3908 Fragments arranged by size: 3908 1344 1327 971 789 140 BbvCI CC'TCA_GC Cuts at: 3615 3615 Size: 8479 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5543 5577 6670 6704 1903 Size: 34 3606 34 1093 34 3678 Fragments arranged by size: 3678 3606 1093 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5001 6371 7581 5001 Size: 1370 1210 5899 Fragments arranged by size: 5899 1370 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8479 BfrBI ATG'CAT Cuts at: 4654 4654 Size: 8479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7090 136 Size: 107 122 71 631 605 5418 1525 Fragments arranged by size: 5418 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5463 8474 5463 Size: 3011 5468 BlpI GC'TnA_GC Cuts at: 5586 5706 5896 5586 Size: 120 190 8169 Fragments arranged by size: 8169 190 120 Bme1580I G_kGCm'C Cuts at: 1790 6540 7696 1790 Size: 4750 1156 2573 Fragments arranged by size: 4750 2573 1156 BmgBI CAC'GTC Cuts at: 1318 4874 1318 Size: 3556 4923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2291 3508 5175 Size: 416 53 83 399 24 874 1217 1667 Cuts at: 5175 6210 7168 442 Size: 1035 958 1753 Fragments arranged by size: 1753 1667 1217 1035 958 874 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8479 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2148 3318 3612 4344 5565 7139 1239 Size: 909 1170 294 732 1221 1574 2579 Fragments arranged by size: 2579 1574 1221 1170 909 732 294 Bpu10I CC'TnA_GC Cuts at: 3615 5711 3615 Size: 2096 6383 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3465 6604 7469 7767 3465 Size: 3139 865 298 4177 Fragments arranged by size: 4177 3139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5388 7142 882 Size: 617 3889 1754 2219 Fragments arranged by size: 3889 2219 1754 617 BsaAI yAC'GTr Cuts at: 493 1204 3886 6270 493 Size: 711 2682 2384 2702 Fragments arranged by size: 2702 2682 2384 711 BsaBI GATnn'nnATC Cuts at: 5462 8473 5462 Size: 3011 5468 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6286 6668 7303 275 Size: 53 83 186 936 4753 382 635 1451 Fragments arranged by size: 4753 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6912 7310 7584 7731 8142 6912 Size: 398 274 147 411 7249 Fragments arranged by size: 7249 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8254 Fragments arranged by size: 8254 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2214 3379 4481 4547 1186 Size: 1028 1165 1102 66 5118 Fragments arranged by size: 5118 1165 1102 1028 66 BseYI C'CCAG_C Cuts at: 7682 8178 7682 Size: 496 7983 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7906 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5929 6690 7718 1540 Size: 275 186 3928 761 1028 2301 Fragments arranged by size: 3928 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3479 3577 6540 6625 7696 3479 Size: 98 2963 85 1071 4262 Fragments arranged by size: 4262 2963 1071 98 85 BsmI GAATG_Cn' Cuts at: 2440 5772 5990 6083 2440 Size: 3332 218 93 4836 Fragments arranged by size: 4836 3332 218 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3479 3577 5592 5798 6540 6625 7696 Size: 399 1689 98 2015 206 742 85 1071 Cuts at: 7696 1391 Size: 2174 Fragments arranged by size: 2174 2015 1689 1071 742 399 206 98 85 BspEI T'CCGG_A Cuts at: 8142 8142 Size: 8479 BspHI T'CATG_A Cuts at: 2691 3022 6368 8098 2691 Size: 331 3346 1730 3072 Fragments arranged by size: 3346 3072 1730 331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5834 844 Size: 4990 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6366 1394 Size: 429 4543 3507 Fragments arranged by size: 4543 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2301 3558 6974 7148 65 Size: 1183 1053 1257 3416 174 1396 Fragments arranged by size: 3416 1396 1257 1183 1053 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2127 3644 7123 7310 1700 Size: 110 317 1517 3479 187 2869 Fragments arranged by size: 3479 2869 1517 317 187 110 BsrGI T'GTAC_A Cuts at: 96 3596 96 Size: 3500 4979 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8479 BssSI C'ACGA_G Cuts at: 4914 6539 7551 4914 Size: 1625 1012 5842 Fragments arranged by size: 5842 1625 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4216 794 Size: 3422 5057 BstBI TT'CG_AA Cuts at: 2422 7283 2422 Size: 4861 3618 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8479 BstXI CCAn_nnnn'nTGG Cuts at: 4853 4853 Size: 8479 BstYI r'GATC_y Cuts at: 1972 4135 5463 5809 6176 6561 6578 8030 8116 Size: 2163 1328 346 367 385 17 1452 86 Cuts at: 8116 8128 8474 1972 Size: 12 346 1977 Fragments arranged by size: 2163 1977 1452 1328 385 367 346 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2209 2304 5388 5661 513 Size: 552 1144 95 3084 273 3331 Fragments arranged by size: 3331 3084 1144 552 273 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2332 3260 487 Size: 683 56 1106 928 5706 Fragments arranged by size: 5706 1106 928 683 56 BtsI GCAGTG_nn' Cuts at: 3943 4214 4226 4343 5995 6789 6809 3943 Size: 271 12 117 1652 794 20 5613 Fragments arranged by size: 5613 1652 794 271 117 20 12 ClaI AT'CG_AT Cuts at: 1554 4006 1554 Size: 2452 6027 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8444 DraI TTT'AAA Cuts at: 4963 5783 4963 Size: 820 7659 DraIII CAC_nnn'GTG Cuts at: 3262 3262 Size: 8479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2632 2645 7486 809 Size: 507 1316 13 4841 1802 Fragments arranged by size: 4841 1802 1316 507 13 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 5926 6815 Size: 54 1425 50 275 18 295 3801 889 Cuts at: 6815 8444 8 Size: 1629 43 Fragments arranged by size: 3801 1629 1425 889 295 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5926 1537 Size: 275 4114 4090 Fragments arranged by size: 4114 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2168 2209 2780 3024 6409 1353 Size: 815 41 571 244 3385 3423 Fragments arranged by size: 3423 3385 815 571 244 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7066 7440 7586 387 Size: 1064 138 5477 374 146 1280 Fragments arranged by size: 5477 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3477 3477 Size: 8479 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1998 2148 2481 2801 3068 3318 3612 Size: 393 366 150 333 320 267 250 294 Cuts at: 3612 4344 4430 5565 6542 7139 7926 8194 1239 Size: 732 86 1135 977 597 787 268 1524 Fragments arranged by size: 1524 1135 977 787 732 597 393 366 333 320 294 268 267 250 150 86 EcoNI CCTnn'n_nnAGG Cuts at: 3537 3537 Size: 8479 EcoO109I rG'GnC_Cy Cuts at: 4441 4820 4441 Size: 379 8100 EcoRI G'AATT_C Cuts at: 5788 6166 5788 Size: 378 8101 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3242 3242 Size: 8479 FseI GG_CCGG'CC Cuts at: 2131 2131 Size: 8479 FspI TGC'GCA Cuts at: 6985 6985 Size: 8479 HaeII r_GCGC'y Cuts at: 1678 7626 1678 Size: 5948 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4823 4855 5171 5203 5311 Size: 32 135 32 3251 32 316 32 108 Cuts at: 5311 5343 7120 7152 7194 7226 1373 Size: 32 1777 32 42 32 2626 Fragments arranged by size: 3251 2626 1777 316 135 108 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3967 4098 4648 5823 6069 669 Size: 988 281 2029 131 550 1175 246 3079 Fragments arranged by size: 3079 2029 1175 988 550 281 246 131 HindIII A'AGCT_T Cuts at: 748 748 Size: 8479 HpaI GTT'AAC Cuts at: 4098 6069 4098 Size: 1971 6508 KpnI G_GTAC'C Cuts at: 3645 5804 3645 Size: 2159 6320 MfeI C'AATT_G Cuts at: 6078 6078 Size: 8479 MluI A'CGCG_T Cuts at: 8355 8355 Size: 8479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2784 7593 7777 1305 Size: 1479 4809 184 2007 Fragments arranged by size: 4809 2007 1479 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6657 Fragments arranged by size: 6657 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 3213 3401 3441 3843 4219 4851 Size: 648 898 1149 188 40 402 376 632 Cuts at: 4851 4941 5534 6437 6796 6955 518 Size: 90 593 903 359 159 2042 Fragments arranged by size: 2042 1149 903 898 648 632 593 402 376 359 188 159 90 40 MspA1I CmG'CkG Cuts at: 1606 3296 3985 4661 5895 6572 7720 7965 1606 Size: 1690 689 676 1234 677 1148 245 2120 Fragments arranged by size: 2120 1690 1234 1148 689 677 676 245 NaeI GCC'GGC Cuts at: 2129 2129 Size: 8479 NcoI C'CATG_G Cuts at: 513 1065 5388 5661 513 Size: 552 4323 273 3331 Fragments arranged by size: 4323 3331 552 273 NdeI CA'TA_TG Cuts at: 387 387 Size: 8479 NgoMIV G'CCGG_C Cuts at: 2127 2127 Size: 8479 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8479 NotI GC'GGCC_GC Cuts at: 5926 5926 Size: 8479 NsiI A_TGCA'T Cuts at: 4656 4656 Size: 8479 NspI r_CATG'y Cuts at: 1132 1229 3446 4654 4658 5558 5837 7382 1132 Size: 97 2217 1208 4 900 279 1545 2229 Fragments arranged by size: 2229 2217 1545 1208 900 279 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8479 PciI A'CATG_T Cuts at: 7378 7378 Size: 8479 PflMI CCAn_nnn'nTGG Cuts at: 1108 4472 4546 1108 Size: 3364 74 5041 Fragments arranged by size: 5041 3364 74 PmeI GTTT'AAAC Cuts at: 5783 5783 Size: 8479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6548 6580 8061 8093 6548 Size: 32 1481 32 6934 Fragments arranged by size: 6934 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4820 4820 Size: 8479 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8479 PsiI TTA'TAA Cuts at: 6049 6049 Size: 8479 PstI C_TGCA'G Cuts at: 830 3069 3757 3914 4221 4666 5737 5831 830 Size: 2239 688 157 307 445 1071 94 3478 Fragments arranged by size: 3478 2239 1071 688 445 307 157 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8479 PvuII CAG'CTG Cuts at: 3296 3985 4661 3296 Size: 689 676 7114 Fragments arranged by size: 7114 689 676 SacI G_AGCT'C Cuts at: 3479 3479 Size: 8479 SalI G'TCGA_C Cuts at: 1936 5821 1936 Size: 3885 4594 SapI GCTCTTCn'nnn_ Cuts at: 2168 2209 2168 Size: 41 8438 SbfI CC_TGCA'GG Cuts at: 5831 5831 Size: 8479 ScaI AGT'ACT Cuts at: 5022 6727 8439 5022 Size: 1705 1712 5062 Fragments arranged by size: 5062 1712 1705 SfcI C'TryA_G Cuts at: 826 1045 3065 3753 3814 3910 3922 4153 4217 Size: 219 2020 688 61 96 12 231 64 Cuts at: 4217 4662 5146 5733 5827 6962 7643 7834 826 Size: 445 484 587 94 1135 681 191 1471 Fragments arranged by size: 2020 1471 1135 688 681 587 484 445 231 219 191 96 94 64 61 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8479 SmaI CCC'GGG Cuts at: 1872 5806 1872 Size: 3934 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3480 6583 7484 7746 820 Size: 197 926 1537 3103 901 262 1553 Fragments arranged by size: 3103 1553 1537 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6270 493 Size: 5777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8479 SphI G_CATG'C Cuts at: 1132 4658 5558 5837 1132 Size: 3526 900 279 3774 Fragments arranged by size: 3774 3526 900 279 SspI AAT'ATT Cuts at: 5 52 6403 5 Size: 47 6351 2081 Fragments arranged by size: 6351 2081 47 StuI AGG'CCT Cuts at: 1517 4586 1517 Size: 3069 5410 StyI C'CwwG_G Cuts at: 513 1065 5388 5661 5918 513 Size: 552 4323 273 257 3074 Fragments arranged by size: 4323 3074 552 273 257 SwaI ATTT'AAAT Cuts at: 4963 4963 Size: 8479 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6506 6523 6676 6861 7306 325 Size: 1255 4926 17 153 185 445 1498 Fragments arranged by size: 4926 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3264 3596 5020 Size: 274 80 33 51 1443 1287 332 1424 Cuts at: 5020 5365 6725 8437 96 Size: 345 1360 1712 138 Fragments arranged by size: 1712 1443 1424 1360 1287 345 332 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8439 8471 8439 Size: 32 8447 Tth111I GACn'n_nGTC Cuts at: 1316 2568 1316 Size: 1252 7227 XbaI T'CTAG_A Cuts at: 3901 5815 3901 Size: 1914 6565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2066 2800 2066 Size: 734 7745 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8479 XmaI C'CCGG_G Cuts at: 1870 5804 1870 Size: 3934 4545 XmnI GAAnn'nnTTC Cuts at: 3183 3534 4806 6608 3183 Size: 351 1272 1802 5054 Fragments arranged by size: 5054 1802 1272 351 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6287 276 Size: 53 83 186 936 4753 2468 Fragments arranged by size: 4753 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI