Restriction map for
pFN21AB0031
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BcnI BglI BlnI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstUI BstXI
BstZ17I Bsu36I CfoI ClaI CpoI
DraI DraII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AclI 2580 AACGTT AA^CGTT
AflII 964 CTTAAG C^TTAAG
AflIII 1906 ACACGT A^C[GA][CT]GT
AhdI 1474 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1089 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1069 ATTAAT AT^TAAT
AsnI 1069 ATTAAT AT^TAAT
AspEI 1474 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 9 GAGCTC G[AT]GC[AT]^C
AvaI 2507 CTCGAG C^[CT]CG[GA]G
AvaII 532 GGTCC G^G[AT]CC
BanII 9 GAGCTC G[GA]GC[CT]^C
BbvCI 275 CCTCAGC CCTCAGC(-5/-2)
BfrI 964 CTTAAG C^TTAAG
BglII 1404 AGATCT A^GATCT
BlpI 1492 GCTCAGC GC^T[ACGT]AGC
BmyI 9 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 1492 GCTCAGC GC^T[ACGT]AGC
BsaAI 1913 CACGTA [CT]AC^GT[GA]
BsiHKAI 9 GAGCTC G[AT]GC[AT]^C
BsoBI 2507 CTCGAG C^[CT]CG[GA]G
Bsp1286I 9 GAGCTC G[AGT]GC[ACT]^C
BsrFI 2175 GCCGGT [GA]^CCGG[CT]
BstBI 1655 TTCGAA TT^CGAA
BstEII 2178 GGTGACC G^GT[ACGT]ACC
BstPI 2178 GGTGACC G^GT[ACGT]ACC
CelII 1492 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2175 GCCGGT [GA]^CCGG[CT]
Cfr13I 532 GGTCC G^G[ACGT]CC
DraIII 130 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1212 CCGTGG C^C[GA][CT]GG
Eam1105I 1474 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2178 GGTGACC G^GT[ACGT]ACC
EcoRV 2086 GATATC GAT^ATC
FauI 2586 GCGGG CCCGC(4/6)
HaeIII 2174 GGCC GG^CC
HapII 2176 CCGG C^CGG
HincII 2162 GTCAAC GT[CT]^[GA]AC
HindII 2162 GTCAAC GT[CT]^[GA]AC
HpaII 2176 CCGG C^CGG
MspI 2176 CCGG C^CGG
MwoI 472 GCCAATGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 779 ACATGC [GA]CATG^[CT]
NspV 1655 TTCGAA TT^CGAA
PaeR7I 2507 CTCGAG C^TCGAG
PshBI 1069 ATTAAT AT^TAAT
Psp1406I 2580 AACGTT AA^CGTT
SacI 9 GAGCTC GAGCT^C
Sau96I 532 GGTCC G^G[ACGT]CC
SfuI 1655 TTCGAA TT^CGAA
XbaI 1950 TCTAGA T^CTAGA
XcmI 2102 CCAAAGATCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2507 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BsaBI 955 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2276 GATCTCCATC
BseRI 12 CTCCTC GAGGAG(10/8)
2659 GAGGAG
BsgI 2333 CTGCAC GTGCAG(16/14)
761 GTGCAG
BsmI 1320 GAATGC GAATGC(1/-1)
2625 GAATGC
BspHI 489 TCATGA T^CATGA
957 TCATGA
EcoRI 65 GAATTC G^AATTC
1652 GAATTC
MamI 955 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2276 GATCTCCATC
NheI 1111 GCTAGC G^CTAGC
2254 GCTAGC
NlaIV 111 GGTTCC GG[ACGT]^[ACGT]CC
407 GGTTCC
PflMI 129 CCACGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1504 CCACAAGCTGG
PleI 1474 GACTC GAGTC(4/5)
1480 GAGTC
PstI 82 CTGCAG CTGCA^G
2414 CTGCAG
RcaI 489 TCATGA T^CATGA
957 TCATGA
SspI 57 AATATT AAT^ATT
1250 AATATT
TaqI 1656 TCGA T^CGA
2508 TCGA
TthHB8I 1656 TCGA T^CGA
2508 TCGA
Van91I 129 CCACGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1504 CCACAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbsI BbvI BfaI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
NdeII NlaIII PvuII RsaI SapI
Sau3AI ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI