(Circular) (Six-base) MAPSORT of: pFN21AB0031.seq Check: 1402 from: 1 to: 7450 pFN21AB0031 7450 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5260 278 Size: 53 83 186 936 3724 2468 Fragments arranged by size: 3724 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4793 1937 Size: 2856 4594 Acc65I G'GTAC_C Cuts at: 4771 4771 Size: 7450 AclI AA'CG_TT Cuts at: 1563 4586 5577 5950 1563 Size: 3023 991 373 3063 Fragments arranged by size: 3063 3023 991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2930 3434 3716 4124 5513 6897 7165 1632 Size: 1298 504 282 408 1389 1384 268 1917 Fragments arranged by size: 1917 1389 1384 1298 504 408 282 268 AflII C'TTAA_G Cuts at: 820 1017 2969 820 Size: 197 1952 5301 Fragments arranged by size: 5301 1952 197 AflIII A'CryG_T Cuts at: 1203 3911 6349 7326 1203 Size: 2708 2438 977 1327 Fragments arranged by size: 2708 2438 1327 977 AgeI A'CCGG_T Cuts at: 6281 6281 Size: 7450 AhdI GACnn_n'nnGTC Cuts at: 3484 6179 3484 Size: 2695 4755 AleI CACnn'nnGTG Cuts at: 1166 2564 1166 Size: 1398 6052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4604 1184 Size: 3420 4030 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2316 2348 2316 Size: 32 7418 AlwNI CAG_nnn'CTG Cuts at: 3099 5193 6765 3099 Size: 2094 1572 3784 Fragments arranged by size: 3784 2094 1572 ApaLI G'TGCA_C Cuts at: 5507 6663 5507 Size: 1156 6294 ApoI r'AATT_y Cuts at: 1493 2070 2393 2502 2515 2974 3657 3883 3896 Size: 577 323 109 13 459 683 226 13 Cuts at: 3896 4595 4759 4985 5137 7246 1493 Size: 699 164 226 152 2109 1697 Fragments arranged by size: 2109 1697 699 683 577 459 323 226 226 164 152 109 13 13 AseI AT'TA_AT Cuts at: 160 1032 3075 6004 160 Size: 872 2043 2929 1606 Fragments arranged by size: 2929 2043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7450 AvaI C'yCGr_G Cuts at: 1870 1943 4512 4775 1870 Size: 73 2569 263 4545 Fragments arranged by size: 4545 2569 263 73 BamHI G'GATC_C Cuts at: 4780 5147 4780 Size: 367 7083 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4771 6226 618 Size: 325 217 627 2984 1455 1842 Fragments arranged by size: 2984 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2018 4769 1391 Size: 627 2751 4072 Fragments arranged by size: 4072 2751 627 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2539 2577 4073 4142 4428 928 Size: 971 640 38 1496 69 286 3950 Fragments arranged by size: 3950 1496 971 640 286 69 38 BbvCI CC'TCA_GC Cuts at: 2281 2281 Size: 7450 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5641 5675 1903 Size: 34 3704 34 3678 Fragments arranged by size: 3704 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5342 6552 5342 Size: 1210 6240 BclI T'GATC_A Cuts at: 1548 1548 Size: 7450 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6061 136 Size: 107 122 71 631 605 4389 1525 Fragments arranged by size: 4389 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3409 7445 3409 Size: 4036 3414 BlpI GC'TnA_GC Cuts at: 3498 4867 3498 Size: 1369 6081 Bme1580I G_kGCm'C Cuts at: 1790 5511 6667 1790 Size: 3721 1156 2573 Fragments arranged by size: 3721 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3914 1318 Size: 2596 4854 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3175 5181 6139 Size: 416 53 83 399 24 1758 2006 958 Cuts at: 6139 442 Size: 1753 Fragments arranged by size: 2006 1758 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3120 4263 1056 Size: 2064 1143 4243 Fragments arranged by size: 4243 2064 1143 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 3510 3540 6110 1239 Size: 950 1321 30 2570 2579 Fragments arranged by size: 2579 2570 1321 950 30 Bpu10I CC'TnA_GC Cuts at: 2281 4700 2281 Size: 2419 5031 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2050 2355 3263 4682 5575 6440 6738 2050 Size: 305 908 1419 893 865 298 2762 Fragments arranged by size: 2762 1419 908 893 865 305 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6113 882 Size: 617 4614 2219 Fragments arranged by size: 4614 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3920 5241 493 Size: 711 2716 1321 2702 Fragments arranged by size: 2716 2702 1321 711 BsaBI GATnn'nnATC Cuts at: 2964 4285 7444 2964 Size: 1321 3159 2970 Fragments arranged by size: 3159 2970 1321 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5257 5639 6274 275 Size: 53 83 186 936 3724 382 635 1451 Fragments arranged by size: 3724 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5883 6281 6555 6702 7113 5883 Size: 398 274 147 411 6220 Fragments arranged by size: 6220 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2235 2265 3885 Size: 30 165 30 624 30 178 30 1620 Cuts at: 3885 3915 1178 Size: 30 4713 Fragments arranged by size: 4713 1620 624 178 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2008 4679 1186 Size: 822 2671 3957 Fragments arranged by size: 3957 2671 822 BseYI C'CCAG_C Cuts at: 6653 7149 6653 Size: 496 6954 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2787 4323 1163 Size: 573 1051 1536 4290 Fragments arranged by size: 4290 1536 1051 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4900 5661 6689 1540 Size: 275 186 2899 761 1028 2301 Fragments arranged by size: 2899 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2018 5511 5596 6667 2018 Size: 3493 85 1071 2801 Fragments arranged by size: 3493 2801 1071 85 BsmI GAATG_Cn' Cuts at: 3331 4636 4961 5054 3331 Size: 1305 325 93 5727 Fragments arranged by size: 5727 1305 325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 4769 5511 5596 6667 1391 Size: 399 228 2751 742 85 1071 2174 Fragments arranged by size: 2751 2174 1071 742 399 228 85 BspEI T'CCGG_A Cuts at: 7113 7113 Size: 7450 BspHI T'CATG_A Cuts at: 2494 2962 5339 7069 2494 Size: 468 2377 1730 2875 Fragments arranged by size: 2875 2377 1730 468 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4805 844 Size: 3961 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5337 1394 Size: 429 3514 3507 Fragments arranged by size: 3514 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5945 6119 65 Size: 1183 4697 174 1396 Fragments arranged by size: 4697 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4180 6094 6281 1700 Size: 110 2370 1914 187 2869 Fragments arranged by size: 2869 2370 1914 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7450 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7450 BssSI C'ACGA_G Cuts at: 5510 6522 5510 Size: 1012 6438 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7450 BstBI TT'CG_AA Cuts at: 3661 6254 3661 Size: 2593 4857 BstEII G'GTnAC_C Cuts at: 1184 4183 1184 Size: 2999 4451 BstYI r'GATC_y Cuts at: 1972 3038 3409 4280 4492 4780 5147 5532 5549 Size: 1066 371 871 212 288 367 385 17 Cuts at: 5549 7001 7087 7099 7445 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1066 871 385 371 367 346 288 212 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3217 513 Size: 552 2152 4746 Fragments arranged by size: 4746 2152 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6711 Fragments arranged by size: 6711 683 56 BtsI GCAGTG_nn' Cuts at: 3217 4966 5760 5780 3217 Size: 1749 794 20 4887 Fragments arranged by size: 4887 1749 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7450 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3093 3128 529 Size: 35 2529 35 4851 Fragments arranged by size: 4851 2529 35 35 DraI TTT'AAA Cuts at: 4754 4754 Size: 7450 DraIII CAC_nnn'GTG Cuts at: 2140 2140 Size: 7450 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6457 809 Size: 507 5141 1802 Fragments arranged by size: 5141 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4897 5786 7415 Size: 54 1425 50 275 18 3067 889 1629 Cuts at: 7415 8 Size: 43 Fragments arranged by size: 3067 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4897 1537 Size: 275 3085 4090 Fragments arranged by size: 4090 3085 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 2241 2643 2712 2754 3438 3537 3955 Size: 653 235 402 69 42 684 99 418 Cuts at: 3955 3966 4119 4143 5380 1353 Size: 11 153 24 1237 3423 Fragments arranged by size: 3423 1237 684 653 418 402 235 153 99 69 42 24 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2120 6037 6411 6557 387 Size: 1064 138 531 3917 374 146 1280 Fragments arranged by size: 3917 1280 1064 531 374 146 138 EcoICRI GAG'CTC Cuts at: 2016 2016 Size: 7450 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2930 3434 3510 3540 3716 4124 Size: 393 557 741 504 76 30 176 408 Cuts at: 4124 5513 6110 6897 7165 1239 Size: 1389 597 787 268 1524 Fragments arranged by size: 1524 1389 787 741 597 557 504 408 393 268 176 76 30 EcoRI G'AATT_C Cuts at: 2070 3657 4759 5137 2070 Size: 1587 1102 378 4383 Fragments arranged by size: 4383 1587 1102 378 EcoRV GAT'ATC Cuts at: 4093 4093 Size: 7450 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4049 4154 4715 4049 Size: 105 561 6784 Fragments arranged by size: 6784 561 105 FspI TGC'GCA Cuts at: 5956 5956 Size: 7450 HaeII r_GCGC'y Cuts at: 1678 6597 1678 Size: 4919 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 2648 2680 3266 Size: 32 135 32 692 32 352 32 586 Cuts at: 3266 3298 3470 3502 3617 3649 4096 4128 4248 Size: 32 172 32 115 32 447 32 120 Cuts at: 4248 4280 6091 6123 6165 6197 1373 Size: 32 1811 32 42 32 2626 Fragments arranged by size: 2626 1811 692 586 447 352 172 135 120 115 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4169 4794 5040 669 Size: 988 281 2231 625 246 3079 Fragments arranged by size: 3079 2231 988 625 281 246 HindIII A'AGCT_T Cuts at: 748 3940 4131 4697 748 Size: 3192 191 566 3501 Fragments arranged by size: 3501 3192 566 191 HpaI GTT'AAC Cuts at: 5040 5040 Size: 7450 KpnI G_GTAC'C Cuts at: 4775 4775 Size: 7450 MfeI C'AATT_G Cuts at: 5049 5049 Size: 7450 MluI A'CGCG_T Cuts at: 7326 7326 Size: 7450 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3603 6564 6748 1305 Size: 2298 2961 184 2007 Fragments arranged by size: 2961 2298 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5628 Fragments arranged by size: 5628 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 3027 3966 5408 5767 5926 518 Size: 648 1398 463 939 1442 359 159 2042 Fragments arranged by size: 2042 1442 1398 939 648 463 359 159 MspA1I CmG'CkG Cuts at: 1606 2241 2921 3502 3718 4866 5543 6691 6936 Size: 635 680 581 216 1148 677 1148 245 Cuts at: 6936 1606 Size: 2120 Fragments arranged by size: 2120 1148 1148 680 677 635 581 245 216 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6898 NdeI CA'TA_TG Cuts at: 387 387 Size: 7450 NheI G'CTAG_C Cuts at: 1052 3116 4259 1052 Size: 2064 1143 4243 Fragments arranged by size: 4243 2064 1143 NotI GC'GGCC_GC Cuts at: 4897 4897 Size: 7450 NspI r_CATG'y Cuts at: 1132 1229 2788 4808 6353 1132 Size: 97 1559 2020 1545 2229 Fragments arranged by size: 2229 2020 1559 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7450 PciI A'CATG_T Cuts at: 6349 6349 Size: 7450 PflMI CCAn_nnn'nTGG Cuts at: 1108 2140 3515 1108 Size: 1032 1375 5043 Fragments arranged by size: 5043 1375 1032 PfoI T'CCnGG_A Cuts at: 2823 2823 Size: 7450 PmeI GTTT'AAAC Cuts at: 4754 4754 Size: 7450 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5519 5551 7032 7064 5519 Size: 32 1481 32 5905 Fragments arranged by size: 5905 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7450 PsiI TTA'TAA Cuts at: 2379 4721 5020 2379 Size: 2342 299 4809 Fragments arranged by size: 4809 2342 299 PstI C_TGCA'G Cuts at: 830 2091 4423 4802 830 Size: 1261 2332 379 3478 Fragments arranged by size: 3478 2332 1261 379 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7450 PvuII CAG'CTG Cuts at: 2921 3502 3718 2921 Size: 581 216 6653 Fragments arranged by size: 6653 581 216 SacI G_AGCT'C Cuts at: 2018 2018 Size: 7450 SalI G'TCGA_C Cuts at: 1936 4792 1936 Size: 2856 4594 SapI GCTCTTCn'nnn_ Cuts at: 2006 3438 3955 2006 Size: 1432 517 5501 Fragments arranged by size: 5501 1432 517 SbfI CC_TGCA'GG Cuts at: 4802 4802 Size: 7450 ScaI AGT'ACT Cuts at: 5698 7410 5698 Size: 1712 5738 SfcI C'TryA_G Cuts at: 826 1045 2087 2889 3186 3793 4419 4798 5933 Size: 219 1042 802 297 607 626 379 1135 Cuts at: 5933 6614 6805 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1042 802 681 626 607 379 297 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7450 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7450 SmaI CCC'GGG Cuts at: 1872 4777 1872 Size: 2905 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2029 2370 2969 3242 4512 4661 Size: 197 926 86 341 599 273 1270 149 Cuts at: 4661 5554 6455 6717 820 Size: 893 901 262 1553 Fragments arranged by size: 1553 1270 926 901 893 599 341 273 262 197 149 86 SnaBI TAC'GTA Cuts at: 493 5241 493 Size: 4748 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7450 SphI G_CATG'C Cuts at: 1132 4808 1132 Size: 3676 3774 SspI AAT'ATT Cuts at: 5 52 2064 3257 5374 5 Size: 47 2012 1193 2117 2081 Fragments arranged by size: 2117 2081 2012 1193 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7450 StyI C'CwwG_G Cuts at: 513 1065 3537 3990 4188 4276 4889 513 Size: 552 2472 453 198 88 613 3074 Fragments arranged by size: 3074 2472 613 552 453 198 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2870 5477 5494 5647 5832 6277 325 Size: 1255 1290 2607 17 153 185 445 1498 Fragments arranged by size: 2607 1498 1290 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4423 4668 4677 Size: 274 80 33 51 1443 2446 245 9 Cuts at: 4677 5696 7408 96 Size: 1019 1712 138 Fragments arranged by size: 2446 1712 1443 1019 274 245 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2235 2267 3371 3403 4167 4199 7410 7442 2235 Size: 32 1104 32 764 32 3211 32 2243 Fragments arranged by size: 3211 2243 1104 764 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7450 XbaI T'CTAG_A Cuts at: 3955 4786 3955 Size: 831 6619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4114 4114 Size: 7450 XhoI C'TCGA_G Cuts at: 1943 4512 1943 Size: 2569 4881 XmaI C'CCGG_G Cuts at: 1870 4775 1870 Size: 2905 4545 XmnI GAAnn'nnTTC Cuts at: 2751 3018 4041 5579 2751 Size: 267 1023 1538 4622 Fragments arranged by size: 4622 1538 1023 267 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5258 276 Size: 53 83 186 936 3724 2468 Fragments arranged by size: 3724 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI