Restriction map for
pFN21AB0031
(Linear) (Six-base) MAPSORT of: KIBB0031.seq Check: 3042 from: 1 to: 2745
KIBB0031.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AclI AA'CG_TT
Cuts at: 0 2581 2745
Size: 2581 164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1429 1711 2119 2745
Size: 925 504 282 408 626
Fragments arranged by size:
925 626 504 408 282
AflII C'TTAA_G
Cuts at: 0 964 2745
Size: 964 1781
AflIII A'CryG_T
Cuts at: 0 1906 2745
Size: 1906 839
AhdI GACnn_n'nnGTC
Cuts at: 0 1479 2745
Size: 1479 1266
AleI CACnn'nnGTG
Cuts at: 0 559 2745
Size: 559 2186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2599 2745
Size: 2599 146
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 311 343 2745
Size: 311 32 2402
Fragments arranged by size:
2402 311 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1094 2745
Size: 1094 1651
ApoI r'AATT_y
Cuts at: 0 65 388 497 510 969 1652 1878 1891
Size: 65 323 109 13 459 683 226 13
Cuts at: 1891 2590 2745
Size: 699 155
Fragments arranged by size:
699 683 459 323 226 155 109 65
13 13
AseI AT'TA_AT
Cuts at: 0 1070 2745
Size: 1070 1675
AvaI C'yCGr_G
Cuts at: 0 2507 2745
Size: 2507 238
BanII G_rGCy'C
Cuts at: 0 13 2745
Size: 13 2732
BbsI GAAGACnn'nnnn_
Cuts at: 0 534 572 2068 2137 2423 2745
Size: 534 38 1496 69 286 322
Fragments arranged by size:
1496 534 322 286 69 38
BbvCI CC'TCA_GC
Cuts at: 0 276 2745
Size: 276 2469
BglII A'GATC_T
Cuts at: 0 1404 2745
Size: 1404 1341
BlpI GC'TnA_GC
Cuts at: 0 1493 2745
Size: 1493 1252
BmgBI CAC'GTC
Cuts at: 0 1909 2745
Size: 1909 836
BmrI ACTGGGnnnn_n'
Cuts at: 0 1170 2745
Size: 1170 1575
BmtI G_CTAG'C
Cuts at: 0 1115 2258 2745
Size: 1115 1143 487
Fragments arranged by size:
1143 1115 487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1505 1535 2745
Size: 184 1321 30 1210
Fragments arranged by size:
1321 1210 184 30
Bpu10I CC'TnA_GC
Cuts at: 0 276 2695 2745
Size: 276 2419 50
Fragments arranged by size:
2419 276 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 350 1258 2677 2745
Size: 45 305 908 1419 68
Fragments arranged by size:
1419 908 305 68 45
BsaAI yAC'GTr
Cuts at: 0 1915 2745
Size: 1915 830
BsaBI GATnn'nnATC
Cuts at: 0 959 2280 2745
Size: 959 1321 465
Fragments arranged by size:
1321 959 465
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 230 260 1880 1910 2745
Size: 22 30 178 30 1620 30 835
Fragments arranged by size:
1620 835 178 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 2674 2745
Size: 3 2671 71
Fragments arranged by size:
2671 71 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 2318 2745
Size: 782 1536 427
Fragments arranged by size:
1536 782 427
BsiHKAI G_wGCw'C
Cuts at: 0 13 2745
Size: 13 2732
BsmI GAATG_Cn'
Cuts at: 0 1326 2631 2745
Size: 1326 1305 114
Fragments arranged by size:
1326 1305 114
Bsp1286I G_dGCh'C
Cuts at: 0 13 2745
Size: 13 2732
BspHI T'CATG_A
Cuts at: 0 489 957 2745
Size: 489 468 1788
Fragments arranged by size:
1788 489 468
BsrFI r'CCGG_y
Cuts at: 0 2175 2745
Size: 2175 570
BstBI TT'CG_AA
Cuts at: 0 1656 2745
Size: 1656 1089
BstEII G'GTnAC_C
Cuts at: 0 2178 2745
Size: 2178 567
BstYI r'GATC_y
Cuts at: 0 1033 1404 2275 2487 2745
Size: 1033 371 871 212 258
Fragments arranged by size:
1033 871 371 258 212
BtgI C'CryG_G
Cuts at: 0 1212 2745
Size: 1212 1533
BtsI GCAGTG_nn'
Cuts at: 0 1212 2745
Size: 1212 1533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1088 1123 2745
Size: 1088 35 1622
Fragments arranged by size:
1622 1088 35
DraIII CAC_nnn'GTG
Cuts at: 0 135 2745
Size: 135 2610
EarI CTCTTCn'nnn_
Cuts at: 0 1 236 638 707 749 1433 1532 1950
Size: 1 235 402 69 42 684 99 418
Cuts at: 1950 1961 2114 2138 2745
Size: 11 153 24 607
Fragments arranged by size:
684 607 418 402 235 153 99 69
42 24 11 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 2745
Size: 115 2630
EcoICRI GAG'CTC
Cuts at: 0 11 2745
Size: 11 2734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 925 1429 1505 1535 1711 2119 2745
Size: 184 741 504 76 30 176 408 626
Fragments arranged by size:
741 626 504 408 184 176 76 30
EcoRI G'AATT_C
Cuts at: 0 65 1652 2745
Size: 65 1587 1093
Fragments arranged by size:
1587 1093 65
EcoRV GAT'ATC
Cuts at: 0 2088 2745
Size: 2088 657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2044 2149 2710 2745
Size: 2044 105 561 35
Fragments arranged by size:
2044 561 105 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 643 675 1261 1293 1465 1497
Size: 259 32 352 32 586 32 172 32
Cuts at: 1497 1612 1644 2091 2123 2243 2275 2745
Size: 115 32 447 32 120 32 470
Fragments arranged by size:
586 470 447 352 259 172 120 115
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2164 2745
Size: 2164 581
HindIII A'AGCT_T
Cuts at: 0 1935 2126 2692 2745
Size: 1935 191 566 53
Fragments arranged by size:
1935 566 191 53
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1598 2745
Size: 1598 1147
MslI CAynn'nnrTG
Cuts at: 0 559 1022 1961 2745
Size: 559 463 939 784
Fragments arranged by size:
939 784 559 463
MspA1I CmG'CkG
Cuts at: 0 236 916 1497 1713 2745
Size: 236 680 581 216 1032
Fragments arranged by size:
1032 680 581 236 216
NheI G'CTAG_C
Cuts at: 0 1111 2254 2745
Size: 1111 1143 491
Fragments arranged by size:
1143 1111 491
NspI r_CATG'y
Cuts at: 0 783 2745
Size: 783 1962
PflMI CCAn_nnn'nTGG
Cuts at: 0 135 1510 2745
Size: 135 1375 1235
Fragments arranged by size:
1375 1235 135
PfoI T'CCnGG_A
Cuts at: 0 818 2745
Size: 818 1927
PsiI TTA'TAA
Cuts at: 0 374 2716 2745
Size: 374 2342 29
Fragments arranged by size:
2342 374 29
PstI C_TGCA'G
Cuts at: 0 86 2418 2745
Size: 86 2332 327
Fragments arranged by size:
2332 327 86
PvuII CAG'CTG
Cuts at: 0 916 1497 1713 2745
Size: 916 581 216 1032
Fragments arranged by size:
1032 916 581 216
SacI G_AGCT'C
Cuts at: 0 13 2745
Size: 13 2732
SapI GCTCTTCn'nnn_
Cuts at: 0 1 1433 1950 2745
Size: 1 1432 517 795
Fragments arranged by size:
1432 795 517 1
SfcI C'TryA_G
Cuts at: 0 82 884 1181 1788 2414 2745
Size: 82 802 297 607 626 331
Fragments arranged by size:
802 626 607 331 297 82
SmlI C'TyrA_G
Cuts at: 0 24 365 964 1237 2507 2656 2745
Size: 24 341 599 273 1270 149 89
Fragments arranged by size:
1270 599 341 273 149 89 24
SspI AAT'ATT
Cuts at: 0 59 1252 2745
Size: 59 1193 1493
Fragments arranged by size:
1493 1193 59
StyI C'CwwG_G
Cuts at: 0 1532 1985 2183 2271 2745
Size: 1532 453 198 88 474
Fragments arranged by size:
1532 474 453 198 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 865 2745
Size: 865 1880
TatI w'GTAC_w
Cuts at: 0 2418 2663 2672 2745
Size: 2418 245 9 73
Fragments arranged by size:
2418 245 73 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 1366 1398 2162 2194 2745
Size: 230 32 1104 32 764 32 551
Fragments arranged by size:
1104 764 551 230 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1950 2745
Size: 1950 795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2109 2745
Size: 2109 636
XhoI C'TCGA_G
Cuts at: 0 2507 2745
Size: 2507 238
XmnI GAAnn'nnTTC
Cuts at: 0 746 1013 2036 2745
Size: 746 267 1023 709
Fragments arranged by size:
1023 746 709 267
Enzymes that do cut:
AclI AcuI AflII AflIII AhdI AleI AlfI AloI
AlwNI ApoI AseI AvaI BanII BbsI BbvCI BglII
BlpI BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrFI BstBI BstEII BstYI BtgI BtsI CspCI DraIII
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI Hin4I
HincII HindIII MmeI MslI MspA1I NheI NspI PflMI
PfoI PsiI PstI PvuII SacI SapI SfcI SmlI
SspI StyI TaqII TatI TstI XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AgeI ApaI ApaLI
AscI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BclI BfrBI BglI Bme1580I BplI BsaI BsaHI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I
BtgZI ClaI DraI DrdI EaeI EagI EcoNI EcoO109I
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XmaI ZraI