(Circular) (Six-base) MAPSORT of: pFN21AA2001.seq Check: 7666 from: 1 to: 6412 pFN21AA2001 6412 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 4222 278 Size: 53 83 186 936 2107 579 2468 Fragments arranged by size: 2468 2107 936 579 186 83 53 AccI GT'mk_AC Cuts at: 1937 2487 3755 1937 Size: 550 1268 4594 Fragments arranged by size: 4594 1268 550 Acc65I G'GTAC_C Cuts at: 3733 3733 Size: 6412 AclI AA'CG_TT Cuts at: 1563 4539 4912 1563 Size: 2976 373 3063 Fragments arranged by size: 3063 2976 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4475 5859 6127 1632 Size: 2843 1384 268 1917 Fragments arranged by size: 2843 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6215 AflIII A'CryG_T Cuts at: 1203 2707 3432 5311 6288 1203 Size: 1504 725 1879 977 1327 Fragments arranged by size: 1879 1504 1327 977 725 AgeI A'CCGG_T Cuts at: 5243 5243 Size: 6412 AhdI GACnn_n'nnGTC Cuts at: 5141 5141 Size: 6412 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6412 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6412 AlwNI CAG_nnn'CTG Cuts at: 2785 3335 4155 5727 2785 Size: 550 820 1572 3470 Fragments arranged by size: 3470 1572 820 550 ApaI G_GGCC'C Cuts at: 2355 2355 Size: 6412 ApaLI G'TGCA_C Cuts at: 4469 5625 4469 Size: 1156 5256 ApoI r'AATT_y Cuts at: 1493 2498 2684 2849 3702 3721 3947 4099 6208 Size: 1005 186 165 853 19 226 152 2109 Cuts at: 6208 1493 Size: 1697 Fragments arranged by size: 2109 1697 1005 853 226 186 165 152 19 AseI AT'TA_AT Cuts at: 160 1032 4966 160 Size: 872 3934 1606 Fragments arranged by size: 3934 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2243 2549 3737 1870 Size: 73 168 132 306 1188 4545 Fragments arranged by size: 4545 1188 306 168 132 73 BamHI G'GATC_C Cuts at: 3325 3742 4109 3325 Size: 417 367 5628 Fragments arranged by size: 5628 417 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2440 2555 3563 3733 5188 Size: 325 217 627 653 115 1008 170 1455 Cuts at: 5188 618 Size: 1842 Fragments arranged by size: 1842 1455 1008 653 627 325 217 170 115 BanII G_rGCy'C Cuts at: 1391 2122 2292 2355 2469 2868 3731 1391 Size: 731 170 63 114 399 863 4072 Fragments arranged by size: 4072 863 731 399 170 114 63 BbeI G_GCGC'C Cuts at: 2559 2559 Size: 6412 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3054 3643 928 Size: 971 1155 589 3697 Fragments arranged by size: 3697 1155 971 589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4603 4637 1903 Size: 34 2666 34 3678 Fragments arranged by size: 3678 2666 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4304 5514 4304 Size: 1210 5202 BclI T'GATC_A Cuts at: 1548 1548 Size: 6412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3561 5023 136 Size: 107 122 71 631 605 1889 1462 1525 Fragments arranged by size: 1889 1525 1462 631 605 122 107 71 BglII A'GATC_T Cuts at: 6407 6407 Size: 6412 BlpI GC'TnA_GC Cuts at: 3829 3829 Size: 6412 Bme1580I G_kGCm'C Cuts at: 1790 2190 2355 4473 5629 1790 Size: 400 165 2118 1156 2573 Fragments arranged by size: 2573 2118 1156 400 165 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6412 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2477 2617 4143 Size: 416 53 83 399 24 1060 140 1526 Cuts at: 4143 5101 442 Size: 958 1753 Fragments arranged by size: 1753 1526 1060 958 416 399 140 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6412 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2140 2468 2985 5072 1239 Size: 851 50 328 517 2087 2579 Fragments arranged by size: 2579 2087 851 517 328 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2720 4537 5402 5700 2720 Size: 1817 865 298 3432 Fragments arranged by size: 3432 1817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2472 2510 3335 5075 882 Size: 617 973 38 825 1740 2219 Fragments arranged by size: 2219 1740 973 825 617 38 BsaAI yAC'GTr Cuts at: 493 1204 2708 4203 493 Size: 711 1504 1495 2702 Fragments arranged by size: 2702 1504 1495 711 BsaBI GATnn'nnATC Cuts at: 6406 6406 Size: 6412 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2556 3640 4219 Size: 53 83 186 936 482 541 1084 579 Cuts at: 4219 4601 5236 275 Size: 382 635 1451 Fragments arranged by size: 1451 1084 936 635 579 541 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4845 5243 5517 5664 6075 4845 Size: 398 274 147 411 5182 Fragments arranged by size: 5182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2469 2499 1178 Size: 30 165 30 1066 30 5091 Fragments arranged by size: 5091 1066 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 3149 3152 3155 3200 3203 3206 3233 Size: 1030 933 3 3 45 3 3 27 Cuts at: 3233 3236 3239 3242 3263 3353 3374 3377 1186 Size: 3 3 3 21 90 21 3 4221 Fragments arranged by size: 4221 1030 933 90 45 27 21 21 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 5615 6111 5615 Size: 496 5916 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5839 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3862 4623 5651 1540 Size: 275 186 1861 761 1028 2301 Fragments arranged by size: 2301 1861 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2868 4473 4558 5629 2868 Size: 1605 85 1071 3651 Fragments arranged by size: 3651 1605 1071 85 BsmI GAATG_Cn' Cuts at: 2095 3923 4016 2095 Size: 1828 93 4491 Fragments arranged by size: 4491 1828 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2190 2292 2355 2469 2868 3731 Size: 399 332 68 102 63 114 399 863 Cuts at: 3731 4473 4558 5629 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 863 742 399 399 332 114 102 85 68 63 BspEI T'CCGG_A Cuts at: 6075 6075 Size: 6412 BspHI T'CATG_A Cuts at: 4301 6031 4301 Size: 1730 4682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3767 844 Size: 2923 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2452 2563 2959 4299 1394 Size: 429 629 111 396 1340 3507 Fragments arranged by size: 3507 1340 629 429 396 111 BsrDI GCAATG_nn' Cuts at: 65 1248 2155 4907 5081 65 Size: 1183 907 2752 174 1396 Fragments arranged by size: 2752 1396 1183 907 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2268 2389 5056 5243 1700 Size: 110 458 121 2667 187 2869 Fragments arranged by size: 2869 2667 458 187 121 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6412 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6412 BssSI C'ACGA_G Cuts at: 3693 4472 5484 3693 Size: 779 1012 4621 Fragments arranged by size: 4621 1012 779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6412 BstBI TT'CG_AA Cuts at: 2971 3634 5216 2971 Size: 663 1582 4167 Fragments arranged by size: 4167 1582 663 BstEII G'GTnAC_C Cuts at: 1184 2620 3464 1184 Size: 1436 844 4132 Fragments arranged by size: 4132 1436 844 BstXI CCAn_nnnn'nTGG Cuts at: 3449 3449 Size: 6412 BstYI r'GATC_y Cuts at: 1972 3325 3742 4109 4494 4511 5963 6049 6061 Size: 1353 417 367 385 17 1452 86 12 Cuts at: 6061 6407 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1353 417 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5860 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5673 Fragments arranged by size: 5673 683 56 BtsI GCAGTG_nn' Cuts at: 3928 4722 4742 3928 Size: 794 20 5598 Fragments arranged by size: 5598 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6412 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6377 DraI TTT'AAA Cuts at: 3716 3716 Size: 6412 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5419 809 Size: 507 4103 1802 Fragments arranged by size: 4103 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2171 2886 Size: 54 1425 50 275 18 294 47 715 Cuts at: 2886 3697 3859 4748 6377 8 Size: 811 162 889 1629 43 Fragments arranged by size: 1629 1425 889 811 715 294 275 162 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3859 1537 Size: 275 2047 4090 Fragments arranged by size: 4090 2047 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3021 3171 3192 4342 1353 Size: 1668 150 21 1150 3423 Fragments arranged by size: 3423 1668 1150 150 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2391 3647 4999 5373 5519 387 Size: 1064 138 802 1256 1352 374 146 1280 Fragments arranged by size: 1352 1280 1256 1064 802 374 146 138 EcoICRI GAG'CTC Cuts at: 2866 2866 Size: 6412 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2140 2468 2985 4475 5072 5859 Size: 393 458 50 328 517 1490 597 787 Cuts at: 5859 6127 1239 Size: 268 1524 Fragments arranged by size: 1524 1490 787 597 517 458 393 328 268 50 EcoNI CCTnn'n_nnAGG Cuts at: 2126 2198 2126 Size: 72 6340 EcoRI G'AATT_C Cuts at: 2498 2684 2849 3702 3721 4099 2498 Size: 186 165 853 19 378 4811 Fragments arranged by size: 4811 853 378 186 165 19 FspI TGC'GCA Cuts at: 4918 4918 Size: 6412 HaeII r_GCGC'y Cuts at: 1678 2396 2559 5559 1678 Size: 718 163 3000 2531 Fragments arranged by size: 3000 2531 718 163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2349 2469 2501 Size: 32 135 32 713 32 32 120 32 Cuts at: 2501 5053 5085 5127 5159 1373 Size: 2552 32 42 32 2626 Fragments arranged by size: 2626 2552 713 135 120 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3756 4002 669 Size: 988 281 939 879 246 3079 Fragments arranged by size: 3079 988 939 879 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6412 HpaI GTT'AAC Cuts at: 4002 4002 Size: 6412 KasI G'GCGC_C Cuts at: 2555 2555 Size: 6412 KpnI G_GTAC'C Cuts at: 3737 3737 Size: 6412 MfeI C'AATT_G Cuts at: 4011 4011 Size: 6412 MluI A'CGCG_T Cuts at: 3432 6288 3432 Size: 2856 3556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2826 5526 5710 1305 Size: 1521 2700 184 2007 Fragments arranged by size: 2700 2007 1521 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2173 10 Size: 54 1425 343 341 4249 Fragments arranged by size: 4249 1425 343 341 54 MslI CAynn'nnrTG Cuts at: 518 1166 4370 4729 4888 518 Size: 648 3204 359 159 2042 Fragments arranged by size: 3204 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2169 2357 2446 3828 4505 5653 5898 1606 Size: 563 188 89 1382 677 1148 245 2120 Fragments arranged by size: 2120 1382 1148 677 563 245 188 89 NaeI GCC'GGC Cuts at: 2270 2391 2270 Size: 121 6291 NarI GG'CG_CC Cuts at: 2556 2556 Size: 6412 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5860 NdeI CA'TA_TG Cuts at: 387 387 Size: 6412 NgoMIV G'CCGG_C Cuts at: 2268 2389 2268 Size: 121 6291 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6412 NotI GC'GGCC_GC Cuts at: 3859 3859 Size: 6412 NspI r_CATG'y Cuts at: 1132 1229 2648 3770 5315 1132 Size: 97 1419 1122 1545 2229 Fragments arranged by size: 2229 1545 1419 1122 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6412 PciI A'CATG_T Cuts at: 5311 5311 Size: 6412 PflMI CCAn_nnn'nTGG Cuts at: 1108 2607 1108 Size: 1499 4913 PfoI T'CCnGG_A Cuts at: 2328 2664 2328 Size: 336 6076 PmeI GTTT'AAAC Cuts at: 3716 3716 Size: 6412 PmlI CAC'GTG Cuts at: 2708 2708 Size: 6412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4481 4513 5994 6026 4481 Size: 32 1481 32 4867 Fragments arranged by size: 4867 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6412 PsiI TTA'TAA Cuts at: 3982 3982 Size: 6412 PspOMI G'GGCC_C Cuts at: 2351 2351 Size: 6412 PstI C_TGCA'G Cuts at: 830 3764 830 Size: 2934 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6412 SacI G_AGCT'C Cuts at: 2868 2868 Size: 6412 SalI G'TCGA_C Cuts at: 1936 3754 1936 Size: 1818 4594 SbfI CC_TGCA'GG Cuts at: 3764 3764 Size: 6412 ScaI AGT'ACT Cuts at: 3032 4660 6372 3032 Size: 1628 1712 3072 Fragments arranged by size: 3072 1712 1628 SfcI C'TryA_G Cuts at: 826 1045 3034 3580 3760 4895 5576 5767 826 Size: 219 1989 546 180 1135 681 191 1471 Fragments arranged by size: 1989 1471 1135 681 546 219 191 180 SfoI GGC'GCC Cuts at: 2557 2557 Size: 6412 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 SgrAI Cr'CCGG_yG Cuts at: 1700 2389 1700 Size: 689 5723 SmaI CCC'GGG Cuts at: 1872 2245 2551 3739 1872 Size: 373 306 1188 4545 Fragments arranged by size: 4545 1188 373 306 SmlI C'TyrA_G Cuts at: 820 1017 1943 2735 4516 5417 5679 820 Size: 197 926 792 1781 901 262 1553 Fragments arranged by size: 1781 1553 926 901 792 262 197 SnaBI TAC'GTA Cuts at: 493 4203 493 Size: 3710 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6412 SphI G_CATG'C Cuts at: 1132 2648 3770 1132 Size: 1516 1122 3774 Fragments arranged by size: 3774 1516 1122 SspI AAT'ATT Cuts at: 5 52 4336 5 Size: 47 4284 2081 Fragments arranged by size: 4284 2081 47 StuI AGG'CCT Cuts at: 1517 2841 3555 1517 Size: 1324 714 4374 Fragments arranged by size: 4374 1324 714 StyI C'CwwG_G Cuts at: 513 1065 2601 3081 3851 513 Size: 552 1536 480 770 3074 Fragments arranged by size: 3074 1536 770 552 480 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2608 2639 3476 4439 4456 4609 4794 Size: 1255 1028 31 837 963 17 153 185 Cuts at: 4794 5239 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1028 963 837 445 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3030 4658 6370 Size: 274 80 33 51 1443 1053 1628 1712 Cuts at: 6370 96 Size: 138 Fragments arranged by size: 1712 1628 1443 1053 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3422 3454 6372 6404 3422 Size: 32 2918 32 3430 Fragments arranged by size: 3430 2918 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2483 1316 Size: 1167 5245 XbaI T'CTAG_A Cuts at: 3748 3748 Size: 6412 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6412 XmaI C'CCGG_G Cuts at: 1870 2243 2549 3737 1870 Size: 373 306 1188 4545 Fragments arranged by size: 4545 1188 373 306 XmnI GAAnn'nnTTC Cuts at: 2238 3555 4541 2238 Size: 1317 986 4109 Fragments arranged by size: 4109 1317 986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 4220 276 Size: 53 83 186 936 2107 579 2468 Fragments arranged by size: 2468 2107 936 579 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI