Restriction map for
pFN21AA1927
(Circular) (Six-base) MAPSORT of: pFN21AA1927.seq Check: 6360 from: 1 to: 8482
pFN21AA1927 8482 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6292 278
Size: 53 83 186 936 4756 2468
Fragments arranged by size:
4756 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3974 4529 5825 1937
Size: 2037 555 1296 4594
Fragments arranged by size:
4594 2037 1296 555
Acc65I G'GTAC_C
Cuts at: 5803 5803
Size: 8482
AclI AA'CG_TT
Cuts at: 1563 6609 6982 1563
Size: 5046 373 3063
Fragments arranged by size:
5046 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 2565 3560 3561 3713 3891 4356 4707
Size: 753 180 995 1 152 178 465 351
Cuts at: 4707 5033 5267 5568 5601 6545 7929 8197 1632
Size: 326 234 301 33 944 1384 268 1917
Fragments arranged by size:
1917 1384 995 944 753 465 351 326
301 268 234 180 178 152 33 1
AfeI AGC'GCT
Cuts at: 5170 5170
Size: 8482
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8285
AflIII A'CryG_T
Cuts at: 1203 7381 8358 1203
Size: 6178 977 1327
Fragments arranged by size:
6178 1327 977
AgeI A'CCGG_T
Cuts at: 7313 7313
Size: 8482
AhdI GACnn_n'nnGTC
Cuts at: 7211 7211
Size: 8482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5121 1184
Size: 3937 4545
AlwNI CAG_nnn'CTG
Cuts at: 2103 3110 5056 5219 5315 5498 5624 6225 7797
Size: 1007 1946 163 96 183 126 601 1572
Cuts at: 7797 2103
Size: 2788
Fragments arranged by size:
2788 1946 1572 1007 601 183 163 126
96
ApaLI G'TGCA_C
Cuts at: 3348 6539 7695 3348
Size: 3191 1156 4135
Fragments arranged by size:
4135 3191 1156
ApoI r'AATT_y
Cuts at: 1493 2467 2550 2601 2911 3138 3962 4909 5029
Size: 974 83 51 310 227 824 947 120
Cuts at: 5029 5289 5791 6017 6169 8278 1493
Size: 260 502 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 947 824 502 310 260
227 226 152 120 83 51
AseI AT'TA_AT
Cuts at: 160 1032 7036 160
Size: 872 6004 1606
Fragments arranged by size:
6004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8482
AvaI C'yCGr_G
Cuts at: 1870 1943 5807 1870
Size: 73 3864 4545
Fragments arranged by size:
4545 3864 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5373 5406 5373
Size: 33 8449
BamHI G'GATC_C
Cuts at: 5812 6179 5812
Size: 367 8115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4453 5803 7258 618
Size: 325 217 627 2666 1350 1455 1842
Fragments arranged by size:
2666 1842 1455 1350 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4037 4099 4244 4887 5557 5801 1391
Size: 2646 62 145 643 670 244 4072
Fragments arranged by size:
4072 2646 670 643 244 145 62
BbeI G_GCGC'C
Cuts at: 4457 4457
Size: 8482
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3663 928
Size: 971 1764 5747
Fragments arranged by size:
5747 1764 971
BbvCI CC'TCA_GC
Cuts at: 5625 5625
Size: 8482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6673 6707 1903
Size: 34 4736 34 3678
Fragments arranged by size:
4736 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6374 7584 6374
Size: 1210 7272
BclI T'GATC_A
Cuts at: 1548 5008 1548
Size: 3460 5022
BfrBI ATG'CAT
Cuts at: 5635 5635
Size: 8482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5085 7093 136
Size: 107 122 71 631 605 3413 2008 1525
Fragments arranged by size:
3413 2008 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8477 8477
Size: 8482
BlpI GC'TnA_GC
Cuts at: 5899 5899
Size: 8482
Bme1580I G_kGCm'C
Cuts at: 1790 2226 3352 6543 7699 1790
Size: 436 1126 3191 1156 2573
Fragments arranged by size:
3191 2573 1156 1126 436
BmgBI CAC'GTC
Cuts at: 1318 2576 1318
Size: 1258 7224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3230 4059 4238
Size: 416 53 83 399 24 1813 829 179
Cuts at: 4238 6213 7171 442
Size: 1975 958 1753
Fragments arranged by size:
1975 1813 1753 958 829 416 399 179
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 4266 4871 5000 5090 5111 5319 5406
Size: 830 2197 605 129 90 21 208 87
Cuts at: 5406 5496 7142 1239
Size: 90 1646 2579
Fragments arranged by size:
2579 2197 1646 830 605 208 129 90
90 87 21
Bpu10I CC'TnA_GC
Cuts at: 5625 5625
Size: 8482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2291 2763 6607 7472 7770 2234
Size: 57 472 3844 865 298 2946
Fragments arranged by size:
3844 2946 865 472 298 57
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4218 4286 7145 882
Size: 617 2719 68 2859 2219
Fragments arranged by size:
2859 2719 2219 617 68
BsaAI yAC'GTr
Cuts at: 493 1204 3503 6273 493
Size: 711 2299 2770 2702
Fragments arranged by size:
2770 2702 2299 711
BsaBI GATnn'nnATC
Cuts at: 8476 8476
Size: 8482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2792 4454 6289 6671
Size: 53 83 186 936 1259 1662 1835 382
Cuts at: 6671 7306 275
Size: 635 1451
Fragments arranged by size:
1835 1662 1451 1259 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6915 7313 7587 7734 8145 6915
Size: 398 274 147 411 7252
Fragments arranged by size:
7252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 4039 4069 4982
Size: 30 165 30 711 30 1895 30 913
Cuts at: 4982 5012 1178
Size: 30 4648
Fragments arranged by size:
4648 1895 913 711 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2171 2174 2183 2186 3432 3722 3725
Size: 868 117 3 9 3 1246 290 3
Cuts at: 3725 4355 5003 5006 5087 5108 5125 1186
Size: 630 648 3 81 21 17 4543
Fragments arranged by size:
4543 1246 868 648 630 290 117 81
21 17 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 5083 7685 8181 5083
Size: 2602 496 5384
Fragments arranged by size:
5384 2602 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5522 1163
Size: 573 3786 4123
Fragments arranged by size:
4123 3786 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5932 6693 7721 1540
Size: 275 186 3931 761 1028 2301
Fragments arranged by size:
3931 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2671 3352 4037 4099 4587 4604 4709 4887 6543
Size: 681 685 62 488 17 105 178 1656
Cuts at: 6543 6628 7699 2671
Size: 85 1071 3454
Fragments arranged by size:
3454 1656 1071 685 681 488 178 105
85 62 17
BsmI GAATG_Cn'
Cuts at: 3127 3517 4475 5993 6086 3127
Size: 390 958 1518 93 5523
Fragments arranged by size:
5523 1518 958 390 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2111 2111
Size: 8482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2671 3352 4037 4099 4244 4587
Size: 399 436 445 681 685 62 145 343
Cuts at: 4587 4604 4709 4887 5557 5801 6543 6628 7699
Size: 17 105 178 670 244 742 85 1071
Cuts at: 7699 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 685 681 670 445 436
399 343 244 178 145 105 85 62
17
BspEI T'CCGG_A
Cuts at: 8145 8145
Size: 8482
BspHI T'CATG_A
Cuts at: 3208 6371 8101 3208
Size: 3163 1730 3589
Fragments arranged by size:
3589 3163 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5837 844
Size: 4993 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5133 6369 1394
Size: 429 3310 1236 3507
Fragments arranged by size:
3507 3310 1236 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4953 6977 7151 65
Size: 1183 3705 2024 174 1396
Fragments arranged by size:
3705 2024 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 2197 7126 7313 1700
Size: 110 200 187 4929 187 2869
Fragments arranged by size:
4929 2869 200 187 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8482
BssHII G'CGCG_C
Cuts at: 1925 2187 1925
Size: 262 8220
BssSI C'ACGA_G
Cuts at: 6542 7554 6542
Size: 1012 7470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3758 794
Size: 2964 5518
BstBI TT'CG_AA
Cuts at: 3405 7286 3405
Size: 3881 4601
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8482
BstYI r'GATC_y
Cuts at: 1972 2125 2477 2539 5812 6179 6564 6581 8033
Size: 153 352 62 3273 367 385 17 1452
Cuts at: 8033 8119 8131 8477 1972
Size: 86 12 346 1977
Fragments arranged by size:
3273 1977 1452 385 367 352 346 153
86 62 17 12
BstZ17I GTA'TAC
Cuts at: 3975 3975
Size: 8482
BtgI C'CryG_G
Cuts at: 513 1065 2004 4065 4457 4965 513
Size: 552 939 2061 392 508 4030
Fragments arranged by size:
4030 2061 939 552 508 392
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7743
Fragments arranged by size:
7743 683 56
BtsI GCAGTG_nn'
Cuts at: 2874 3307 3535 3958 4030 4772 5998 6792 6812
Size: 433 228 423 72 742 1226 794 20
Cuts at: 6812 2874
Size: 4544
Fragments arranged by size:
4544 1226 794 742 433 423 228 72
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8447
DraI TTT'AAA
Cuts at: 5786 5786
Size: 8482
DraIII CAC_nnn'GTG
Cuts at: 3653 3653
Size: 8482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7489 809
Size: 507 6173 1802
Fragments arranged by size:
6173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2421 2751 5929
Size: 54 1425 50 275 18 591 330 3178
Cuts at: 5929 6818 8447 8
Size: 889 1629 43
Fragments arranged by size:
3178 1629 1425 889 591 330 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5929 1537
Size: 275 4117 4090
Fragments arranged by size:
4117 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2206 3408 3732 3873 4066 4332 4741 6412
Size: 853 1202 324 141 193 266 409 1671
Cuts at: 6412 1353
Size: 3423
Fragments arranged by size:
3423 1671 1202 853 409 324 266 193
141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 2051 4915 7069 7443 7589
Size: 1064 138 456 6 2864 2154 374 146
Cuts at: 7589 387
Size: 1280
Fragments arranged by size:
2864 2154 1280 1064 456 374 146 138
6
EcoICRI GAG'CTC
Cuts at: 4035 4097 4885 4035
Size: 62 788 7632
Fragments arranged by size:
7632 788 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2385 2565 3560 3561 3713 3891
Size: 393 437 316 180 995 1 152 178
Cuts at: 3891 4266 4356 4707 4871 5000 5033 5090 5111
Size: 375 90 351 164 129 33 57 21
Cuts at: 5111 5267 5319 5406 5496 5568 5601 6545 7142
Size: 156 52 87 90 72 33 944 597
Cuts at: 7142 7929 8197 1239
Size: 787 268 1524
Fragments arranged by size:
1524 995 944 787 597 437 393 375
351 316 268 180 178 164 156 152
129 90 90 87 72 57 52 33
33 21 1
EcoO109I rG'GnC_Cy
Cuts at: 2176 4572 4808 5053 5570 2176
Size: 2396 236 245 517 5088
Fragments arranged by size:
5088 2396 517 245 236
EcoRI G'AATT_C
Cuts at: 2467 5791 6169 2467
Size: 3324 378 4780
Fragments arranged by size:
4780 3324 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2570 3418 2570
Size: 848 7634
FspI TGC'GCA
Cuts at: 5114 6988 5114
Size: 1874 6608
FspAI rTGC'GCAy
Cuts at: 5114 5114
Size: 8482
HaeII r_GCGC'y
Cuts at: 1678 4457 5080 5172 7629 1678
Size: 2779 623 92 2457 2531
Fragments arranged by size:
2779 2531 2457 623 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2114 2142 2146 2336
Size: 32 135 32 538 4 28 4 190
Cuts at: 2336 2368 3014 3046 3545 3577 3698 3730 4019
Size: 32 646 32 499 32 121 32 289
Cuts at: 4019 4051 4367 4399 5165 5197 7123 7155 7197
Size: 32 316 32 766 32 1926 32 42
Cuts at: 7197 7229 1373
Size: 32 2626
Fragments arranged by size:
2626 1926 766 646 538 499 316 289
190 135 121 42 32 32 32 32
32 32 32 32 32 32 32 28
4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 5826 6072 669
Size: 988 281 3888 246 3079
Fragments arranged by size:
3888 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8482
HpaI GTT'AAC
Cuts at: 6072 6072
Size: 8482
KasI G'GCGC_C
Cuts at: 4453 4453
Size: 8482
KpnI G_GTAC'C
Cuts at: 5807 5807
Size: 8482
MfeI C'AATT_G
Cuts at: 6081 6081
Size: 8482
MluI A'CGCG_T
Cuts at: 8358 8358
Size: 8482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2502 7596 7780 1305
Size: 789 408 5094 184 2007
Fragments arranged by size:
5094 2007 789 408 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2423 2753 10
Size: 54 1425 343 591 330 5739
Fragments arranged by size:
5739 1425 591 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2379 2385 4680 4733 5150 5640 6440
Size: 648 1213 6 2295 53 417 490 800
Cuts at: 6440 6799 6958 518
Size: 359 159 2042
Fragments arranged by size:
2295 2042 1213 800 648 490 417 359
159 53 6
MspA1I CmG'CkG
Cuts at: 1606 2194 2204 2966 3110 4459 5068 5546 5621
Size: 588 10 762 144 1349 609 478 75
Cuts at: 5621 5898 6575 7723 7968 1606
Size: 277 677 1148 245 2120
Fragments arranged by size:
2120 1349 1148 762 677 609 588 478
277 245 144 75 10
NaeI GCC'GGC
Cuts at: 2199 2199
Size: 8482
NarI GG'CG_CC
Cuts at: 4454 4454
Size: 8482
NcoI C'CATG_G
Cuts at: 513 1065 2004 4065 4965 513
Size: 552 939 2061 900 4030
Fragments arranged by size:
4030 2061 939 900 552
NdeI CA'TA_TG
Cuts at: 387 4980 387
Size: 4593 3889
NgoMIV G'CCGG_C
Cuts at: 2197 2197
Size: 8482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8482
NotI GC'GGCC_GC
Cuts at: 5929 5929
Size: 8482
NruI TCG'CGA
Cuts at: 2240 2240
Size: 8482
NsiI A_TGCA'T
Cuts at: 5637 5637
Size: 8482
NspI r_CATG'y
Cuts at: 1132 1229 4685 5119 5840 7385 1132
Size: 97 3456 434 721 1545 2229
Fragments arranged by size:
3456 2229 1545 721 434 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8482
PciI A'CATG_T
Cuts at: 7381 7381
Size: 8482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8482
PfoI T'CCnGG_A
Cuts at: 2786 4282 5296 2786
Size: 1496 1014 5972
Fragments arranged by size:
5972 1496 1014
PmeI GTTT'AAAC
Cuts at: 5786 5786
Size: 8482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6551 6583 8064 8096 6551
Size: 32 1481 32 6937
Fragments arranged by size:
6937 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 4808 5053 5570 2176
Size: 2632 245 517 5088
Fragments arranged by size:
5088 2632 517 245
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8482
PsiI TTA'TAA
Cuts at: 2559 6052 2559
Size: 3493 4989
PstI C_TGCA'G
Cuts at: 830 2802 4779 5067 5834 830
Size: 1972 1977 288 767 3478
Fragments arranged by size:
3478 1977 1972 767 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8482
PvuII CAG'CTG
Cuts at: 2194 2966 3110 5068 5546 5621 2194
Size: 772 144 1958 478 75 5055
Fragments arranged by size:
5055 1958 772 478 144 75
SacI G_AGCT'C
Cuts at: 4037 4099 4887 4037
Size: 62 788 7632
Fragments arranged by size:
7632 788 62
SacII CC_GC'GG
Cuts at: 4460 4460
Size: 8482
SalI G'TCGA_C
Cuts at: 1936 5824 1936
Size: 3888 4594
SbfI CC_TGCA'GG
Cuts at: 5834 5834
Size: 8482
ScaI AGT'ACT
Cuts at: 4837 6730 8442 4837
Size: 1893 1712 4877
Fragments arranged by size:
4877 1893 1712
SfcI C'TryA_G
Cuts at: 826 1045 2798 3075 3549 3838 4775 5063 5830
Size: 219 1753 277 474 289 937 288 767
Cuts at: 5830 6965 7646 7837 826
Size: 1135 681 191 1471
Fragments arranged by size:
1753 1471 1135 937 767 681 474 289
288 277 219 191
SfoI GGC'GCC
Cuts at: 4455 4455
Size: 8482
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8482
SmaI CCC'GGG
Cuts at: 1872 5809 1872
Size: 3937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 2306 2778 6586 7487 7749
Size: 197 926 306 57 472 3808 901 262
Cuts at: 7749 820
Size: 1553
Fragments arranged by size:
3808 1553 926 901 472 306 262 197
57
SnaBI TAC'GTA
Cuts at: 493 3503 6273 493
Size: 3010 2770 2702
Fragments arranged by size:
3010 2770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8482
SphI G_CATG'C
Cuts at: 1132 5119 5840 1132
Size: 3987 721 3774
Fragments arranged by size:
3987 3774 721
SspI AAT'ATT
Cuts at: 5 52 6406 5
Size: 47 6354 2081
Fragments arranged by size:
6354 2081 47
StuI AGG'CCT
Cuts at: 1517 2081 1517
Size: 564 7918
StyI C'CwwG_G
Cuts at: 513 1065 2004 3169 4065 4965 5921 513
Size: 552 939 1165 896 900 956 3074
Fragments arranged by size:
3074 1165 956 939 900 896 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3212 6509 6526 6679 6864 7309 325
Size: 1255 1632 3297 17 153 185 445 1498
Fragments arranged by size:
3297 1632 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2300 4326 4835
Size: 274 80 33 51 1443 323 2026 509
Cuts at: 4835 6728 8440 96
Size: 1893 1712 138
Fragments arranged by size:
2026 1893 1712 1443 509 323 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2476 2508 3826 3858 8442 8474 2036
Size: 32 408 32 1318 32 4584 32 2044
Fragments arranged by size:
4584 2044 1318 408 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8482
XbaI T'CTAG_A
Cuts at: 5486 5818 5486
Size: 332 8150
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8482
XmaI C'CCGG_G
Cuts at: 1870 5807 1870
Size: 3937 4545
XmnI GAAnn'nnTTC
Cuts at: 2550 3417 5447 6611 2550
Size: 867 2030 1164 4421
Fragments arranged by size:
4421 2030 1164 867
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6290 276
Size: 53 83 186 936 4756 2468
Fragments arranged by size:
4756 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BplI BsiWI BstXI
Bsu36I EcoNI EcoRV FseI PacI PmlI PspOMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI
XcmI