(Circular) (Six-base) MAPSORT of: pFN21AA1901.seq Check: 140 from: 1 to: 8479 pFN21AA1901 8479 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6289 278 Size: 53 83 186 936 4753 2468 Fragments arranged by size: 4753 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5822 1937 Size: 3885 4594 Acc65I G'GTAC_C Cuts at: 5800 5800 Size: 8479 AclI AA'CG_TT Cuts at: 1563 6606 6979 1563 Size: 5043 373 3063 Fragments arranged by size: 5043 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2663 2871 3792 3848 4020 4074 4454 4866 Size: 1031 208 921 56 172 54 380 412 Cuts at: 4866 5307 6542 7926 8194 1632 Size: 441 1235 1384 268 1917 Fragments arranged by size: 1917 1384 1235 1031 921 441 412 380 268 208 172 56 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8282 AflIII A'CryG_T Cuts at: 1203 2356 5153 7378 8355 1203 Size: 1153 2797 2225 977 1327 Fragments arranged by size: 2797 2225 1327 1153 977 AgeI A'CCGG_T Cuts at: 7310 7310 Size: 8479 AhdI GACnn_n'nnGTC Cuts at: 2747 4623 7208 2747 Size: 1876 2585 4018 Fragments arranged by size: 4018 2585 1876 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5736 1184 Size: 4552 3927 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4947 4979 4947 Size: 32 8447 AlwNI CAG_nnn'CTG Cuts at: 4433 6222 7794 4433 Size: 1789 1572 5118 Fragments arranged by size: 5118 1789 1572 ApaLI G'TGCA_C Cuts at: 6536 7692 6536 Size: 1156 7323 ApoI r'AATT_y Cuts at: 1493 2373 2825 3110 3438 3706 4675 4707 5089 Size: 880 452 285 328 268 969 32 382 Cuts at: 5089 5606 5667 5788 6014 6166 8275 1493 Size: 517 61 121 226 152 2109 1697 Fragments arranged by size: 2109 1697 969 880 517 452 382 328 285 268 226 152 121 61 32 AseI AT'TA_AT Cuts at: 160 1032 7033 160 Size: 872 6001 1606 Fragments arranged by size: 6001 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8479 AvaI C'yCGr_G Cuts at: 1870 1943 3321 3651 4080 4628 4858 5804 1870 Size: 73 1378 330 429 548 230 946 4545 Fragments arranged by size: 4545 1378 946 548 429 330 230 73 AvrII C'CTAG_G Cuts at: 2732 2732 Size: 8479 BamHI G'GATC_C Cuts at: 5809 6176 5809 Size: 367 8112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5800 7255 618 Size: 325 217 627 4013 1455 1842 Fragments arranged by size: 4013 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2569 5798 1391 Size: 1178 3229 4072 Fragments arranged by size: 4072 3229 1178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3416 5295 928 Size: 971 1517 1879 4112 Fragments arranged by size: 4112 1879 1517 971 BbvCI CC'TCA_GC Cuts at: 2808 2808 Size: 8479 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 6670 6704 1903 Size: 34 1862 34 2837 34 3678 Fragments arranged by size: 3678 2837 1862 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6371 7581 6371 Size: 1210 7269 BclI T'GATC_A Cuts at: 1548 1548 Size: 8479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7090 136 Size: 107 122 71 631 605 5418 1525 Fragments arranged by size: 5418 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4016 5167 8474 4016 Size: 1151 3307 4021 Fragments arranged by size: 4021 3307 1151 BlpI GC'TnA_GC Cuts at: 5896 5896 Size: 8479 Bme1580I G_kGCm'C Cuts at: 1790 5482 6540 7696 1790 Size: 3692 1058 1156 2573 Fragments arranged by size: 3692 2573 1156 1058 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8479 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6210 7168 442 Size: 416 53 83 399 24 4793 958 1753 Fragments arranged by size: 4793 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3584 1056 Size: 2528 5951 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3021 3078 3468 4569 5603 7139 1239 Size: 1782 57 390 1101 1034 1536 2579 Fragments arranged by size: 2579 1782 1536 1101 1034 390 57 Bpu10I CC'TnA_GC Cuts at: 2808 2808 Size: 8479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2101 4155 4989 6604 7469 7767 2101 Size: 2054 834 1615 865 298 2813 Fragments arranged by size: 2813 2054 1615 865 834 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7142 882 Size: 617 5643 2219 Fragments arranged by size: 5643 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3848 6270 493 Size: 711 2644 2422 2702 Fragments arranged by size: 2702 2644 2422 711 BsaBI GATnn'nnATC Cuts at: 5460 8473 5460 Size: 3013 5466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6286 6668 7303 275 Size: 53 83 186 936 4753 382 635 1451 Fragments arranged by size: 4753 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4951 6912 7310 7584 7731 8142 4951 Size: 1961 398 274 147 411 5288 Fragments arranged by size: 5288 1961 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3286 3316 1178 Size: 30 165 30 1883 30 6341 Fragments arranged by size: 6341 1883 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2796 1186 Size: 1610 6869 BseYI C'CCAG_C Cuts at: 5465 7682 8178 5465 Size: 2217 496 5766 Fragments arranged by size: 5766 2217 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7906 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5929 6690 7718 1540 Size: 275 186 3928 761 1028 2301 Fragments arranged by size: 3928 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6540 6625 7696 6540 Size: 85 1071 7323 Fragments arranged by size: 7323 1071 85 BsmI GAATG_Cn' Cuts at: 5543 5990 6083 5543 Size: 447 93 7939 Fragments arranged by size: 7939 447 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2569 5482 5798 6540 6625 7696 1391 Size: 399 779 2913 316 742 85 1071 2174 Fragments arranged by size: 2913 2174 1071 779 742 399 316 85 BspEI T'CCGG_A Cuts at: 8142 8142 Size: 8479 BspHI T'CATG_A Cuts at: 4225 5641 6368 8098 4225 Size: 1416 727 1730 4606 Fragments arranged by size: 4606 1730 1416 727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3470 5834 844 Size: 2626 2364 3489 Fragments arranged by size: 3489 2626 2364 BsrBI CCG'CTC Cuts at: 1394 1823 6366 1394 Size: 429 4543 3507 Fragments arranged by size: 4543 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6974 7148 65 Size: 1183 5726 174 1396 Fragments arranged by size: 5726 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7123 7310 1700 Size: 110 5313 187 2869 Fragments arranged by size: 5313 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2332 2359 2594 96 Size: 2236 27 235 5981 Fragments arranged by size: 5981 2236 235 27 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8479 BssSI C'ACGA_G Cuts at: 6539 7551 6539 Size: 1012 7467 BstAPI GCAn_nnn'nTGC Cuts at: 794 3851 794 Size: 3057 5422 BstBI TT'CG_AA Cuts at: 2846 7283 2846 Size: 4437 4042 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8479 BstYI r'GATC_y Cuts at: 1972 2263 2657 4016 4394 5167 5809 6176 6561 Size: 291 394 1359 378 773 642 367 385 Cuts at: 6561 6578 8030 8116 8128 8474 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1359 773 642 394 385 378 367 346 291 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6988 Fragments arranged by size: 6988 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7740 Fragments arranged by size: 7740 683 56 BtsI GCAGTG_nn' Cuts at: 3005 3292 5476 5995 6789 6809 3005 Size: 287 2184 519 794 20 4675 Fragments arranged by size: 4675 2184 794 519 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3724 3759 529 Size: 35 3160 35 5249 Fragments arranged by size: 5249 3160 35 35 DraI TTT'AAA Cuts at: 2722 5783 2722 Size: 3061 5418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7486 809 Size: 507 6170 1802 Fragments arranged by size: 6170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5926 6815 8444 Size: 54 1425 50 275 18 4096 889 1629 Cuts at: 8444 8 Size: 43 Fragments arranged by size: 4096 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5926 1537 Size: 275 4114 4090 Fragments arranged by size: 4114 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3038 3603 3786 3915 4089 4399 5385 6409 Size: 1685 565 183 129 174 310 986 1024 Cuts at: 6409 1353 Size: 3423 Fragments arranged by size: 3423 1685 1024 986 565 310 183 174 129 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7066 7440 7586 387 Size: 1064 138 5477 374 146 1280 Fragments arranged by size: 5477 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2663 2871 3021 3078 3468 3792 3848 Size: 393 1031 208 150 57 390 324 56 Cuts at: 3848 4020 4074 4454 4569 4866 5307 5603 6542 Size: 172 54 380 115 297 441 296 939 Cuts at: 6542 7139 7926 8194 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1031 939 787 597 441 393 390 380 324 297 296 268 208 172 150 115 57 56 54 EcoO109I rG'GnC_Cy Cuts at: 2499 4385 5273 2499 Size: 1886 888 5705 Fragments arranged by size: 5705 1886 888 EcoRI G'AATT_C Cuts at: 3438 5089 5788 6166 3438 Size: 1651 699 378 5751 Fragments arranged by size: 5751 1651 699 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2078 2901 3755 5110 5249 2078 Size: 823 854 1355 139 5308 Fragments arranged by size: 5308 1355 854 823 139 FspI TGC'GCA Cuts at: 6985 6985 Size: 8479 HaeII r_GCGC'y Cuts at: 1678 3721 7626 1678 Size: 2043 3905 2531 Fragments arranged by size: 3905 2531 2043 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 2729 2733 2761 Size: 32 135 32 806 32 319 4 28 Cuts at: 2761 2765 3286 3318 4609 4641 4841 4873 5563 Size: 4 521 32 1291 32 200 32 690 Cuts at: 5563 5595 7120 7152 7194 7226 1373 Size: 32 1525 32 42 32 2626 Fragments arranged by size: 2626 1525 1291 806 690 521 319 200 135 42 32 32 32 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2752 4298 4323 5823 6069 669 Size: 988 281 814 1546 25 1500 246 3079 Fragments arranged by size: 3079 1546 1500 988 814 281 246 25 HindIII A'AGCT_T Cuts at: 748 748 Size: 8479 HpaI GTT'AAC Cuts at: 4323 6069 4323 Size: 1746 6733 KpnI G_GTAC'C Cuts at: 5804 5804 Size: 8479 MfeI C'AATT_G Cuts at: 6078 6078 Size: 8479 MluI A'CGCG_T Cuts at: 8355 8355 Size: 8479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3177 4448 7593 7777 1305 Size: 1872 1271 3145 184 2007 Fragments arranged by size: 3145 2007 1872 1271 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6657 Fragments arranged by size: 6657 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 2361 3342 3840 4230 5646 5718 Size: 648 1074 121 981 498 390 1416 72 Cuts at: 5718 6437 6796 6955 518 Size: 719 359 159 2042 Fragments arranged by size: 2042 1416 1074 981 719 648 498 390 359 159 121 72 MspA1I CmG'CkG Cuts at: 1606 5895 6572 7720 7965 1606 Size: 4289 677 1148 245 2120 Fragments arranged by size: 4289 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6988 Fragments arranged by size: 6988 939 552 NdeI CA'TA_TG Cuts at: 387 3666 387 Size: 3279 5200 NheI G'CTAG_C Cuts at: 1052 3580 1052 Size: 2528 5951 NotI GC'GGCC_GC Cuts at: 5926 5926 Size: 8479 NspI r_CATG'y Cuts at: 1132 1229 2360 2609 5157 5837 7382 1132 Size: 97 1131 249 2548 680 1545 2229 Fragments arranged by size: 2548 2229 1545 1131 680 249 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8479 PciI A'CATG_T Cuts at: 2356 5153 7378 2356 Size: 2797 2225 3457 Fragments arranged by size: 3457 2797 2225 PflMI CCAn_nnn'nTGG Cuts at: 1108 4972 1108 Size: 3864 4615 PfoI T'CCnGG_A Cuts at: 3457 3457 Size: 8479 PmeI GTTT'AAAC Cuts at: 5783 5783 Size: 8479 PmlI CAC'GTG Cuts at: 3848 3848 Size: 8479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6548 6580 8061 8093 6548 Size: 32 1481 32 6934 Fragments arranged by size: 6934 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2499 4385 5273 2499 Size: 1886 888 5705 Fragments arranged by size: 5705 1886 888 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8479 PsiI TTA'TAA Cuts at: 6049 6049 Size: 8479 PspXI vC'TCGA_Gb Cuts at: 3651 3651 Size: 8479 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5202 5234 5202 Size: 32 8447 PstI C_TGCA'G Cuts at: 830 3481 3621 5334 5831 830 Size: 2651 140 1713 497 3478 Fragments arranged by size: 3478 2651 1713 497 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8479 SalI G'TCGA_C Cuts at: 1936 5821 1936 Size: 3885 4594 SanDI GG'GwC_CC Cuts at: 2499 2499 Size: 8479 SapI GCTCTTCn'nnn_ Cuts at: 3915 4399 3915 Size: 484 7995 SbfI CC_TGCA'GG Cuts at: 5831 5831 Size: 8479 ScaI AGT'ACT Cuts at: 2470 4864 6727 8439 2470 Size: 2394 1863 1712 2510 Fragments arranged by size: 2510 2394 1863 1712 SexAI A'CCwGG_T Cuts at: 2634 5365 2634 Size: 2731 5748 SfcI C'TryA_G Cuts at: 826 1045 2024 2073 2472 3297 3477 3585 3617 Size: 219 979 49 399 825 180 108 32 Cuts at: 3617 3701 5330 5827 6962 7643 7834 826 Size: 84 1629 497 1135 681 191 1471 Fragments arranged by size: 1629 1471 1135 979 825 681 497 399 219 191 180 108 84 49 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8479 SmaI CCC'GGG Cuts at: 1872 4082 5806 1872 Size: 2210 1724 4545 Fragments arranged by size: 4545 2210 1724 SmlI C'TyrA_G Cuts at: 820 1017 1943 2116 3321 3651 4170 4858 5004 Size: 197 926 173 1205 330 519 688 146 Cuts at: 5004 6583 7484 7746 820 Size: 1579 901 262 1553 Fragments arranged by size: 1579 1553 1205 926 901 688 519 330 262 197 173 146 SnaBI TAC'GTA Cuts at: 493 6270 493 Size: 5777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8479 SphI G_CATG'C Cuts at: 1132 5837 1132 Size: 4705 3774 SspI AAT'ATT Cuts at: 5 52 2191 5662 5692 6403 5 Size: 47 2139 3471 30 711 2081 Fragments arranged by size: 3471 2139 2081 711 47 30 StuI AGG'CCT Cuts at: 1517 5336 1517 Size: 3819 4660 StyI C'CwwG_G Cuts at: 513 1065 2004 2732 5918 513 Size: 552 939 728 3186 3074 Fragments arranged by size: 3186 3074 939 728 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6506 6523 6676 6861 7306 325 Size: 1255 4926 17 153 185 445 1498 Fragments arranged by size: 4926 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2332 2359 2428 Size: 274 80 33 51 1443 355 27 69 Cuts at: 2428 2468 2594 3856 4530 4561 4862 6725 8437 Size: 40 126 1262 674 31 301 1863 1712 Cuts at: 8437 96 Size: 138 Fragments arranged by size: 1863 1712 1443 1262 674 355 301 274 138 126 80 69 51 40 33 31 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8439 8471 8439 Size: 32 8447 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8479 XbaI T'CTAG_A Cuts at: 5815 5815 Size: 8479 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4407 4407 Size: 8479 XhoI C'TCGA_G Cuts at: 1943 3321 3651 4858 1943 Size: 1378 330 1207 5564 Fragments arranged by size: 5564 1378 1207 330 XmaI C'CCGG_G Cuts at: 1870 4080 5804 1870 Size: 2210 1724 4545 Fragments arranged by size: 4545 2210 1724 XmnI GAAnn'nnTTC Cuts at: 2796 4140 6608 2796 Size: 1344 2468 4667 Fragments arranged by size: 4667 2468 1344 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6287 276 Size: 53 83 186 936 4753 2468 Fragments arranged by size: 4753 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI